Labshake search
Citations for Thermo Fisher :
2951 - 3000 of 6840 citations for H D Glu amc oh since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cancer Biology 2022Quote: ... Glioma stem cells were pulsed every 72 h with EGF/FGF and then disassociated with Accutase (ThermoFisher) at room temperature for 10 min ...
-
bioRxiv - Cancer Biology 2023Quote: Cells were seeded on 6-well plates and transfected 24 h later using Lipofectamine 3000 (Invitrogen, L3000150), according to the manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2023Quote: ... Goat anti-mouse IgG (H+L) Alexa Fluor 647 (1:500, Thermo Fisher Scientific, A-21235, RRID:AB_2535804), Goat anti-rabbit IgG (H+L ...
-
bioRxiv - Molecular Biology 2023Quote: ... click chemistry reactions were performed for 1.5 h in the dark (10 μM Biotin-azide [Thermo Fisher, B10184] ...
-
bioRxiv - Bioengineering 2023Quote: ... the brain sections were incubated for 1 h with goat anti-human IgG Alexa Fluor 647 (Invitrogen) and goat anti-mouse IgG Alexa Fluor 488 (1:500 ...
-
bioRxiv - Cell Biology 2023Quote: ... we used the Maxima H Minus First Strand cDNA Synthesis Kit including DNA digestion (Thermo Fisher Scientific) and oligo (dT)18 primer and random hexamer primer ...
-
bioRxiv - Cell Biology 2022Quote: ... Alexa Fluor 350 donkey anti-rabbit IgG (H+L) (1:250 dilution, A-10039, Thermo Fisher Scientific).
-
bioRxiv - Molecular Biology 2023Quote: siRNA transfections were performed on HEK293T cells at 16 h post-culture using Lipofectamine RNAiMAX (Life Technologies) according to the manufactureŕs instructions ...
-
bioRxiv - Immunology 2023Quote: ... 12μl RNA was reverse transcribed with Maxima H Minus First Strand cDNA Synthesis Kit (Thermo Fisher Scientific) and oligo(dT ...
-
bioRxiv - Cell Biology 2023Quote: ... Secondary antibodies used for IF were: donkey anti-mouse IgG (H+L) Alexa Fluor 488 (ThermoFisher, A21202), goat anti-rabbit Alexa Fluor 633 (ThermoFisher ...
-
bioRxiv - Cell Biology 2023Quote: ... anti-rabbit IgG (H+L) cross-adsorbed Alexa Fluor 405 goat polyclonal (Invitrogen, #A-31556, 1:250) and anti-mouse IgG (H+L ...
-
bioRxiv - Biochemistry 2023Quote: ... for 1 h at room temperature and imaged after incubation with NBT/BCIP substrate solution (Thermo Scientific). Membranes were washed after detection ...
-
bioRxiv - Molecular Biology 2023Quote: cDNA was synthesized using the Revert Aid H minus First Strand cDNA synthesis kit (Thermo Fisher Scientific) according to the manufacturers’ instructions ...
-
bioRxiv - Molecular Biology 2023Quote: ... The transfection mixtures were replaced at 6 h post-transfection by 293 SFM II expression medium (Gibco), supplemented with sodium bicarbonate (3.7 g/L) ...
-
bioRxiv - Developmental Biology 2023Quote: ... After an additional 24 h the cells were detached with Trypsin EDTA 0.25% (Cat# 25200056, Thermo Scientific) and subjected to three washed with PBS (Cat #14190094 ...
-
bioRxiv - Neuroscience 2023Quote: ... The sections were then incubated in alexa fluor 488 ex anti-rabbit IgG (H+L) (A11008, Invitrogen) diluted 1:1000 in PBST in order to detect the primary antibody for 1 h at room temperature before being mounted on glass slides and coverslipped with Fluoromount-G (0100-01 ...
-
bioRxiv - Neuroscience 2023Quote: ... PDGFRα panning plates were coated with goat-anti-rat IgG (H+L) (7.5 µg/mL; ThermoFisher Scientific) in PBS with 0.2% BSA and rat anti-PDGFRα (1.5 µg/mL in PBS ...
-
bioRxiv - Microbiology 2023Quote: ... Secreted antibodies were detected with 1µg/mL goat anti-human IgG (H+L)-Alexa Fluor 594 (Invitrogen), which was added to the cell culture media used to resuspend the antigen-coated beads.
-
Linear ubiquitination at damaged lysosomes induces local NF-κB activation and controls cell survivalbioRxiv - Cell Biology 2023Quote: ... Secondary antibodies for immunofluorescence were goat anti-mouse IgG (H+L) Alexa Fluor™ 555 (Thermo Fisher Scientific Cat# A-21422 ...
-
bioRxiv - Cell Biology 2023Quote: ... mRNA was transcribed to cDNA using the RevertAid H Minus Reverse Transcriptase kit (#EP0451, Thermo Fisher Scientific) according to manufacturer’s instructions ...
-
bioRxiv - Physiology 2023Quote: CD4+ T cells were harvested after 72 h of anti-CD3 activation on cytoslides (Thermo scientific, 5991056) using a Shandon cytospin centrifuge (400 rpm for 4 min at RT) ...
-
bioRxiv - Microbiology 2023Quote: ... The membrane was blocked for 1 h at RT with nucleic acid detection blocking buffer (Life Technologies), then probed for 1 h at RT with streptavidin-HRP 1:5,000 (Genescript ...
-
bioRxiv - Neuroscience 2024Quote: ... followed by one hour incubation with donkey anti-mouse IgG [H+L]/ AF 550 (SA5-10167, Invitrogen) and one hour incubation with mouse anti-Beta-Amyloid MOAB-2/AF 750 (NBP2-13075AF750 ...
-
bioRxiv - Cell Biology 2024Quote: ... and goat anti-guinea pig IgG (H+L) Alexa Fluor 555 (Invitrogen, A21435; U-ExM, 1:250).
-
bioRxiv - Cell Biology 2024Quote: ... followed by 1-h incubation with Alexa 488 and/or Alexa 546-labeled secondary antibodies (Molecular Probes). Nuclei were stained with 4,6-diamino-2-phenylindole (DAPI ...
-
bioRxiv - Cancer Biology 2024Quote: ... 60 or 72 h before adding 50 µL of a solution of 1 mg/mL XTT (ThermoFisher) and continuing the incubation for 4 h ...
-
bioRxiv - Cell Biology 2024Quote: ... and goat anti-rabbit IgG (H + L) highly cross-adsorbed secondary antibody Alexa Fluor 594 (Invitrogen/ThermoFisher). Nuclei were stained with DAPI before mounting (Sigma ...
-
bioRxiv - Microbiology 2024Quote: ... This was followed by incubation of 1 h with goat anti-mouse DyeLight 800 (1:5000; Invitrogen) secondary antibody conjugated with a near fluorescent dye in 5% (w/v ...
-
bioRxiv - Immunology 2024Quote: ... at 1.5x106 cells per well for 1 h at 37°C in warm RPMI-1640 medium (GIBCO). The medium was then supplemented to a final concentration of 10% FBS (Sigma-Aldrich ...
-
bioRxiv - Molecular Biology 2024Quote: ... Samples were stained with Alexa-Fluor 647 anti-rabbit (H+L) secondary antibody (ThermoFisher Scientific #A-31571) for 45 min at 4°C ...
-
bioRxiv - Biochemistry 2024Quote: ... the linear amplicon was ligated using the T4 Ligase enzyme for 4 h at 37 ℃ (Thermo Scientific) and then followed by heat-inactivation at 65 ℃ for 10 min ...
-
bioRxiv - Biochemistry 2024Quote: ... cells were incubated with secondary antibodies (Alexa-conjugated IgG H+L series, Thermofisher, diluted to 1:500) and 1 μg/mL DAPI (Sigma ...
-
bioRxiv - Systems Biology 2024Quote: ... 3.7 U/μl DNA polymerase I and 0.2 U/μl Ribonuclease H (all from ThermoFisher Scientific, USA)) for 2h at 16°C ...
-
bioRxiv - Immunology 2024Quote: ... followed by anti-IgG (H+L) highly Cross-Adsorbed secondary antibody (Alexa Fluor 555) (Thermo Fisher Scientific). After one hr of incubation in the dark ...
-
bioRxiv - Genetics 2024Quote: ... cDNA was synthesized from RNA using a Maxima H-First Strand cDNA Synthesis Kit (Thermo Scientific™). Quantitative PCRs were performed on three independent replicates in duplicate using 10 ng/µL cDNA template ...
-
bioRxiv - Immunology 2024Quote: ... and Goat anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody Alexa Fluor 647 (Thermo Fisher Scientific) to assess CM purity as described by Zhang et al.39 Flow cytometry was performed on a Cytoflex Flow Cytometer (Beckman Coulter ...
-
bioRxiv - Cell Biology 2024Quote: ... Postnuclear supernatants were incubated for 2 h with anti-HA magnetic beads (PierceTM, Thermo Fisher Scientific #88837), or anti-FLAG magnetic beads (Sigma-Aldrich #M8823 ...
-
bioRxiv - Cell Biology 2024Quote: ... and goat anti-rabbit IgG (H + L) highly cross-adsorbed secondary antibody Alexa Fluor 594 (Invitrogen/ThermoFisher). Nuclei were stained with DAPI before mounting (Sigma ...
-
bioRxiv - Molecular Biology 2024Quote: ... Total RNA (500 ng) was reverse transcribed by Maxima H Minus Reverse Transcriptase (Thermo Scientific, Carlsbad, CA). Plus-strand genomic RNA ...
-
bioRxiv - Microbiology 2024Quote: ... neurons were incubated for 1□h in Alexa Fluor 488-conjugated secondary antibodies for multicolor imaging (Invitrogen). Nuclei were stained with Hoechst 33258 (Life Technologies) ...
-
bioRxiv - Microbiology 2024Quote: ... 7E6 BMDMs were seeded and treated for 24 h with 10 U/ml murine IFNγ (Gibco PMC4031) or left untreated prior to infection ...
-
bioRxiv - Neuroscience 2024Quote: ... Secondary antibodies included donkey anti-mouse IgG(H+L) Alexa Fluor 594 (Invitrogen, #A- 11058, 1:500), donkey anti-goat IgG(H+L ...
-
bioRxiv - Neuroscience 2024Quote: ... total RNA was reverse-transcribed using Maxima H Minus First Strand cDNA Synthesis Kit (Thermo Fisher K1652). The resultant cDNA was mixed with primers (Integrated DNA Technology ...
-
bioRxiv - Molecular Biology 2024Quote: ... and goat anti-mouse IgG (H+L) Cross-Adsorbed Secondary Antibody DyLight™ 680 (Invitrogen, Cat # 35519) (1:10,000) ...
-
bioRxiv - Cell Biology 2024Quote: ... for 2 h and counterstaining with 2 μg/ml Hoechst 33342 (H1399, Thermo Fisher Scientific, MA, USA) for 15 min ...
-
bioRxiv - Immunology 2024Quote: ... and incubated with a goat anti-human IgG (H+L) AF647 secondary antibody (Thermo Fisher A- 21445) for 1 hour at RT in the dark ...
-
bioRxiv - Physiology 2024Quote: ... we reverse-transcribed 500 ng RNA with the Maxima™ H Master Mix 5x (Thermo Fisher Scientific) in a total volume of 10 μl according to manufacturer’s instructions (10 min 25 °C ...
-
bioRxiv - Plant Biology 2024Quote: ... migrated 1 h at 190 V in Novex™ Nupage™ MOPS or tris-acetate (Invitrogen™) buffer according to protein molecular weight and transferred to nitrocellulose membrane using semidry transfer technique ...
-
bioRxiv - Immunology 2024Quote: ... and the secondary antibody was an AF555-labeled goat anti-rabbit IgG (H + L) antibody (Invitrogen, US). The fluorescence was imaged using a laser confocal microscope (Leica-STELLARIS ...
-
bioRxiv - Microbiology 2024Quote: ... Alexa fluor 546-Goat anti-Guinea Pig IgG (H+L) (Thermo Fisher Scientific, Cat. no. A-11074), and Alexa fluor 488-conjugated Donkey anti-Sheep IgG (H+L ...