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Citations for Thermo Fisher :
3101 - 3150 of 5129 citations for MRPL54 Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... Peptides were analysed by on-line nanoflow LC using the Ultimate 3000 nano system (Dionex/Thermo Fisher Scientific). Samples were loaded onto a trap column (Acclaim PepMap 100 ...
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bioRxiv - Molecular Biology 2023Quote: ... Peptides were separated on an in-house 50 cm column (360 μm od, 75 μm id, Fisher Scientific) packed with ReproSil-Pur C18 (120 Å pore size ...
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bioRxiv - Cell Biology 2023Quote: ... peptide samples were desalted and subjected to analysis using an Orbitrap Fusion Lumos mass spectrometer (Thermo Fisher Scientific). For perturbation experiments (either coRIC or total proteome) ...
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bioRxiv - Neuroscience 2023Quote: ... Peptides were separated by reverse-phase chromatography on an 25-cm Easy Spray column (Thermo Fisher Scientific, USA) connected to an EASY-nLC 1200 instrument (Thermo Fisher Scientific) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Aliquots of 30 µg peptides were labelled using TMTpro 18-plex isobaric mass tagging reagents (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... Peptides were loaded onto a trap column (PepMap 100 C18, 5 μm, 5 × 0.3 mm, Thermo Fisher Scientific) at a flow rate of 10 μL/min using 0.1% TFA as loading buffer ...
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bioRxiv - Systems Biology 2024Quote: ... 100 μg peptide mixture for each sample was labeled using TMT reagent according to the instructions (Thermo Scientific). The labeled peptides were separated by High pH Reversed-Phase Peptide Fractionation Kit (Thermo Scientific ...
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bioRxiv - Bioengineering 2024Quote: ... The membrane permeability of the peptide was determined by using the LIVE/DEAD BacLight Bacterial Viability Kit (Invitrogen). S ...
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bioRxiv - Cell Biology 2024Quote: ... but peptides were gradient eluted from the column directly to an Orbitrap Q Exactive mass spectrometer (Thermo Fisher) using a 155 min acetonitrile gradient from 5 to 35 % B in 120 min followed by a ramp to 45% B in 10 min and 100% B in another 10 min with a hold at 100% B for 10 min (A=2% acetonitrile in 0.5% acetic acid ...
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bioRxiv - Systems Biology 2024Quote: ... Approximately 0.6 μg of peptides were loaded on a C18 precolumn (PepMap100, 5 μm, 300 Å, ThermoFisher Scientific) and separated on a PepMap RSLC C18 column (50 cm × 75 μm ...
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bioRxiv - Microbiology 2023Quote: ... Peptides were trapped on a µPrecolumn C18 PepMap100 (5μm, 100 Å, 300 μm×5mm, ThermoFisher Scientific, Reinach, Switzerland) and separated by backflush on a C18 column (5 μm ...
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bioRxiv - Microbiology 2023Quote: ... Peptide separation was carried out using an Acclaim PepMap trap column (75 μm × 2 cm; Thermo Fisher Scientific) and an EASY-Spray ES902 column (75 μm × 250 mm ...
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bioRxiv - Cell Biology 2023Quote: Desalted SILAC-labeled peptides were analyzed on a Fusion Lumos mass spectrometer (Thermo Fisher Scientific, San Jose, CA) equipped with a Thermo EASY-nLC 1200 LC system (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... The resulting tryptic peptides in 10 μL solution was loaded onto a 2-cm trap column (Thermo Scientific) and separated on a 50-cm EASY-Spray analytical column (Thermo Scientific ...
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bioRxiv - Plant Biology 2023Quote: Peptides were analyzed with 5 different MS methods on a Fusion Lumos Tribrid mass spectrometer (Thermo Fisher Scientific), summarized in Supplementary Dataset S7 ...
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bioRxiv - Cell Biology 2023Quote: ... The peptides were analyzed with an Orbitrap Fusion Tribrid mass spectrometer (Thermo Fisher Scientific, San Jose, CA, USA) in data-dependent mode ...
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bioRxiv - Microbiology 2023Quote: ... biotinylated Pol II CTD mimic peptide (20 µg) were immobilised on 10 µl streptavidin agarose resin (Thermo Scientific) for 2 hours at 4 °C in binding buffer (10 mM HEPES [PAA catalogue no ...
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bioRxiv - Systems Biology 2024Quote: ... peptide mixtures were separated by a reversed-phase chromatography (Dionex Ultimate 3000 NCS-3500RS Nano, Thermo Fisher Scientific) coupled on-line to an Orbitrap Exploris 480 mass spectrometer (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2024Quote: Peptide samples from a 24-hour experiment were injected on a Dionex Ultimate 3000 RSLC (Thermo Fisher Scientific) connected to an Q Exactive Hybrid Quadrupole-Orbitrap mass spectrometer (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2023Quote: ... Peptides were trapped on a 2 cm nanoViper precolumn (i.d. 75 μm; C18, Acclaim PepMapTM 100, Thermo Scientific) with buffer A (2:98 MeCN:H2O in 0.1% formic acid ...
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bioRxiv - Microbiology 2023Quote: Peptides were separated on a C18 nano-column (PepMap 100 C18 series. Thermo Fisher Scientific. Waltham, MA, USA) using an Ultimate 3000 nano-HPLC ...
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bioRxiv - Microbiology 2024Quote: TMT-labelled tryptic peptides were subjected to HpRP fractionation using an Ultimate 3000 RSLC UHPLC system (Thermo Fisher) equipped with a 2.1 mm internal diameter x 25 cm long ...
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bioRxiv - Cancer Biology 2024Quote: ... Acidified eluted peptides were analyzed on a Q Exactive HF mass spectrometer (Thermo Fisher Scientific, Waltham, MA, USA) in the data-dependent mode ...
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bioRxiv - Cancer Biology 2024Quote: ... Peptide extraction was performed using the EasyPep™ MS Sample Prep Kits (Thermo Fisher Scientific Inc, Massachusetts, USA), following the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... Peptides were gradient eluted with a flowrate of 200nL/min into an Orbitrap Eclipse Tribrid (Thermo Fisher Scientific) mass spectrometer ...
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bioRxiv - Developmental Biology 2024Quote: ... On-line peptide separation was performed on a 15-cm EASY-Spray™ C18 column (ES75150PN, Thermo Fisher) by applying a linear gradient of increasing ACN concentration at a flowrate of 200 nL/min ...
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bioRxiv - Cell Biology 2024Quote: ... The resulting peptides were extracted from gel fragments and analyzed using Orbitrap Fusion Lumos mass spectrometer (Thermo Scientific) combined with UltiMate 3000 RSLC nano-flow HPLC (Thermo Scientific) ...
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bioRxiv - Plant Biology 2024Quote: ... Peptides were separated on a 25-cm PepMap C18 column packed with 2-μm particles (Thermo Fisher Scientific) using the mobile buffer consisted of 0.1% FA in ultra-pure water with an eluting buffer of 0.1% FA in 100% ACN with a 60 min linear gradient of 5 to 25% ACN/0.1% FA at a flow rate of 300 nl/min.
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bioRxiv - Molecular Biology 2024Quote: ... Peptides were separated using an Ultimate 3000 nano rapid separation liquid chromatography system (Dionex, ThermoFisher Scientific, MA, USA) equipped with a trapping cartridge (Precolumn C18 PepMap100 ...
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bioRxiv - Molecular Biology 2024Quote: ... 4 µl of the reconstituted peptides were separated on an EASY-nLC™ 1200 chromatography system (Thermo Scientific) coupled to a Q Exactive HF-X Orbitrap LC-MS/MS System (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2024Quote: ... Peptides were separated on a 25-cm PepMap C18 column packed with 2-μm particles (Thermo Fisher Scientific) using the mobile buffer consisted of 0.1% FA in ultra-pure water with an eluting buffer of 0.1% FA in 100% ACN with a 90 min linear gradient of 11 to 37% ACN/0.1% FA at a flow rate of 300 nl/min.
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bioRxiv - Physiology 2024Quote: ... Peptides (360 ng) were loaded (Acclaim PepMap100 C18 3µm beads with 100 Å pore-size, Thermo Fisher Scientific) and separated (1.9-µm particle size C18 ...
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bioRxiv - Neuroscience 2024Quote: ... 700 fmol of standard synthetic AQUA peptides per mg of protein amount (Technologies, Thermo Fisher Scientific, Waltham, USA) were added to the endogenous peptide mixture and peptides were dried in a centrifugal Savant SpeedVac (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2024Quote: ... After purification using SepPak C18 columns (Waters; WAT054955) the peptides were TMT-labeled (Thermo Fisher Scientific; 90061;TH266884) and fractionated with the High pH Reversed-phase Fractionation Kit (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2024Quote: ... The peptides were analyzed by an Orbitrap Fusion Lumos tribrid mass spectrometer with an ESI source (ThermoFisher Scientific) coupled online to the nano-LC and operated in positive polarity ...
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bioRxiv - Cancer Biology 2024Quote: ... suspended in Formic Acid (FA) 0.1% and dosed with the quantitative fluorometric peptide assay (ref. 23290, Thermo Scientific).
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bioRxiv - Microbiology 2024Quote: ... Peptide sequences were determined by matching protein databases with the acquired fragmentation pattern by Sequest (Thermo Fisher Scientific). All databases include a reversed version of all the sequences ...
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bioRxiv - Microbiology 2024Quote: ... Peptides were separated by reversed-phase chromatography using a 50-cm MicroPac Nano C18 column (Thermo Fisher Scientific) with mobile phases of 0.1% formic acid in water and 0.1% formic acid in acetonitrile ...
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bioRxiv - Synthetic Biology 2024Quote: LC-MS/MS analyses of the digested peptides were performed on an EASY-nLC 1200 (Thermo Fisher Scientific) coupled to a Q Exactive HF hybrid quadrupole-Orbitrap mass spectrometer (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2024Quote: ... peptides directly sprayed into an Orbitrap Fusion Lumos mass spectrometer equipped with a nanoFlex ion source (ThermoFisher Scientific). Full scan MS spectra (350-1400 m/z ...
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bioRxiv - Molecular Biology 2024Quote: ... Tagged ribosomes were then eluted by incubating the beads with 200 µg/mL HA peptide (Thermo Fisher Scientific) for 15 minutes at 30°C with constant agitation ...
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bioRxiv - Microbiology 2024Quote: ... Peptides were analysed by liquid chromatography coupled to mass spectrometry (LC-MS) using a Easy1200 nLC (Thermo Scientific) coupled to a tribrid Orbitrap Eclipse with FAIMSpro mass spectrometer (Thermo Scientific).
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bioRxiv - Cell Biology 2024Quote: The purified peptides were analyzed using the reverse phase liquid chromatography system Easy-nLC 1200 UHPLC (Thermo Scientific) coupled to an Orbitrap Fusion Lumos Tribrid Mass Spectrometer (Thermo Scientific ...
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bioRxiv - Plant Biology 2024Quote: ... Peptides were analysed by nano HPLC-MS/MS with a Ultimate 3000 nanoRSLC (Thermo Fisher Scientific, Darmstadt, Germany) operated in a two- column setup (2 cm PepMap C18 trap ...
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bioRxiv - Cell Biology 2020Quote: ... in PBS before staining with primary antibodies diluted in blocking buffer and a mixture of 1:1000 of Alexa Fluor 488-conjugated goat anti-mouse secondary antibody (Invitrogen), 1:5000 Dapi (Sigma-Aldrich) ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were washed X3 with PBS and incubated in the dark for 60 minutes at RT with Alexa Fluor 594 goat anti-rabbit IgG (H+L) (1:500 dilution in blocking buffer) (Invitrogen). Coverslips were washed X3 with PBS ...
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bioRxiv - Developmental Biology 2021Quote: ... 50 μm thick vibratome sections were postfixed with 4% PFA for 15 min at RT followed by blocking in 10% normal horse serum (Gibco) and 0.2% Triton in PBS overnight at 4°C ...
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bioRxiv - Developmental Biology 2021Quote: ... we incubated the samples in blocking solution with fluorescent dye-conjugated secondary antibodies and the nuclear counterstain DAPI (ThermoFisher Scientific) if applicable at 4 °C overnight ...
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bioRxiv - Developmental Biology 2020Quote: ... Developmental Studies Hybridoma Bank) was used at 1:30 in blocking solution and goat α-mouse Alexa568 secondary antibody (Invitrogen) at 1:1,000 in blocking solution ...
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bioRxiv - Biochemistry 2022Quote: ... Plates were washed three times with 0.05% PBST and loaded with a blocking solution of 0.5% BSA (Fisher Scientific, BP9706100) in 0.1% PBST for 1 hour at room temperature ...