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Citations for Thermo Fisher :
2951 - 3000 of 5129 citations for MRPL54 Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... The eluted peptides were sprayed directly by electrospray ionization into an Orbitrap Exploris 480 mass spectrometer (Thermo Scientific). Mass spectrometry measurement was conducted in data-dependent acquisition mode using a top15 method with one full scan (resolution ...
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bioRxiv - Biochemistry 2021Quote: ... The resulting cyclic peptide-mRNA:cDNA library was counter-selected 3 times against His Pull-Down Dynabeads (Thermo Fisher), and affinity selected against 200 nM bead-immobilised Retromer for 30 min ...
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bioRxiv - Bioengineering 2021Quote: ... Celsus Laboratories) was reacted with peptide-hydrazides using 1-ethyl-3-[3-dimethylaminopropyl] carbodiimide hydrochloride (EDC, ThermoFisher Scientific) in 0.1 M MES [2-(N-morpholino)ethanesulfonic acid] buffer with 8 M urea (Sigma ...
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bioRxiv - Molecular Biology 2022Quote: ... Shotgun proteomic analyses of the digested peptides were performed by LTQ-Orbitrap Velos mass spectrometer (Thermo Fisher Scientific) coupled with Dina-2A nanoflow liquid chromatography system (KYA Technologies) ...
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bioRxiv - Neuroscience 2022Quote: ... before nanoLC-ms/ms analysis of the peptides using an Orbitrap Fusion Lumos mass spectrometer (Thermo Fisher Scientific). Sub-cortical white matter lesions from five individuals with relapsing remitting multiple sclerosis (RRMS ...
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bioRxiv - Molecular Biology 2022Quote: ... IPs were washed 8X with NET2 and bound proteins eluted with 3XFLAG peptides and extracted with Trizol (Thermofisher). Eluted RNA was reverse transcribed and amplified with spliceosomal RNA specific primers (Supplemental Table 4)
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bioRxiv - Cancer Biology 2022Quote: ... The separated peptides were analyzed with an online coupled Q Exactive HF-X Mass Spectrometer (Thermo Fisher Scientific) using the data-dependent Top 10 method ...
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bioRxiv - Microbiology 2022Quote: ... Eluting peptides were analyzed in a QExactive Plus hybrid quadrupole-Orbitrap mass spectrometer (Thermo Fisher Scientific, CA, USA).
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bioRxiv - Microbiology 2022Quote: ... Peptide separation was performed using a reverse-phase analytical column (Acclaim PepMap RSLC, 0.075 × 250 mm, Thermo Scientific) with a linear gradient of 4 %-36 % solvent B (0.1 % FA in 98 % ACN ...
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bioRxiv - Microbiology 2022Quote: ... Samples were separated into eight fractions using a High-pH Reversed-Phase Peptide Fractionation Kit (Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Systems Biology 2022Quote: SILAC peptide mixtures (500 ng) were subjected to nLC-MS/MS on a EASY-nLC 1200 (Thermo Fisher) coupled to a Orbitrap Eclipse Tribrid mass spectrometer (Thermo Fisher) ...
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bioRxiv - Genetics 2022Quote: Desalted peptide samples were analysed by a reversed-phase capillary nano liquid chromatography system (Ultimate 3000, Thermo Scientific) connected to a Q Exactive HF mass spectrometer (Thermo Scientific) ...
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bioRxiv - Cancer Biology 2022Quote: ... Peptides were separated on a 50 cm 75 μm inner diameter EasySpray ES903 microcapillary column (Thermo Fisher Scientific). Peptides were separated over a 190 min gradient of 6 - 27% acetonitrile in 1.0% formic acid with a flow rate of 300 nL/min.
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bioRxiv - Genetics 2022Quote: ... Desalted peptides were injected in an EASY-SprayTM PepMapTM RSLC C18 50cm X 75cm ID column (Thermo Scientific) connected to the Orbitrap FusionTM TribridTM ...
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bioRxiv - Microbiology 2019Quote: ... Eluting peptides were directly analyzed by electrospray in an orbitrap mass spectrometer (Q-Exactive Plus, Thermo Fisher Scientific) by scanning a window of 400-2000 m/z with resolution of 70,000 at m/z 400 ...
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bioRxiv - Systems Biology 2019Quote: Peptides were analysed by on-line nanoflow LC using the Ultimate 3000 nano system (Dionex/Thermo Fisher Scientific) system coupled to a Q-Exactive HF mass spectrometer (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2019Quote: ... The resultant peptides were run for 30 minutes on a Dionex LC and Orbitrap Fusion 1 (Thermo Scientific) for LC-MS/MS ...
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bioRxiv - Developmental Biology 2020Quote: ... peptides were separated on C18 material on an Ultimate 3000 rapid separation liquid chromatography system (Thermo Fisher Scientific) using a one-hour gradient and injected into a QExactive plus mass spectrometer (Thermo Fisher Scientific ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Peptides were labelled with 0.8 mg of TMT label (TMT10plex™ Isobaric Label Reagent Set, Thermo Fisher Scientific). Labelled samples were pooled ...
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bioRxiv - Cell Biology 2020Quote: ... The resulting peptides were extracted from gel fragments and analyzed with Orbitrap Fusion Lumos Mass Spectrometer (Thermo Scientific) combined with UltiMate 3000 RSLC nano-flow HPLC system (Thermo Scientific ...
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bioRxiv - Biochemistry 2019Quote: ... Analysis of the peptides was performed via reversed-phase capillary liquid chromatography (Ultimate 3000 nanoLC system (Thermo Scientific)) coupled to an Orbitrap Elite mass spectrometer (Thermo Scientific) ...
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Structure of the Human Signal Peptidase Complex Reveals the Determinants for Signal Peptide CleavagebioRxiv - Biochemistry 2020Quote: ... Peptides were first trapped on the trapping cartridge (PepMap100 C18, 5 μm, 5 mm × 300 μm; Thermo Scientific) prior to separation on an analytical column (Poroshell EC-C18 ...
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bioRxiv - Bioengineering 2020Quote: ... The final concentrations of peptide-patterned DNA origami were measured at UV-Vis A260 on Nanodrop (Thermo Scientific).
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bioRxiv - Molecular Biology 2021Quote: ... Peptides were trapped on a µPrecolumn C18 PepMap100 (5μm, 100 Å, 300 μm×5mm, ThermoFisher Scientific, Reinach, Switzerland) and separated by backflush on a C18 column (5 μm ...
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bioRxiv - Cancer Biology 2020Quote: ... Peptides were reconstituted in 10 μl of H2O and reacted with 80 μg of TMT10plex (Thermo Scientific, #90111) [46] label reagent dissolved in 4 μl of acetonitrile for 1 h at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... Peptide extracts were analysed by nanoLC-ESI-MS/MS using the Ultimate 3000/Orbitrap Fusion instrumentation (Thermo Scientific) with a 60 min LC separation on a 25 cm column.
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bioRxiv - Synthetic Biology 2021Quote: ... Twenty micrograms of each peptide were incubated with 20 µl of pre-washed Dynabeads M-280 Streptavidin (Invitrogen) at RT for 1 hour ...
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bioRxiv - Immunology 2020Quote: ... The digested peptides were analyzed on Orbitrap Fusion Tribrid mass spectrometer interfaced with Easy-nLC-1200 (Thermo Scientific). Each fraction was reconstituted in 0.1% formic acid and loaded onto the trap column (75 um×2cm ...
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bioRxiv - Immunology 2020Quote: The digested peptides were analyzed on Orbitrap Fusion Tribrid mass spectrometer interfaced with Easy-nLC-1200 (Thermo Scientific). Each fraction was reconstituted in 0.1% formic acid and loaded onto the trap column (75 um×2cm ...
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bioRxiv - Molecular Biology 2021Quote: ... labeling peptides were performed according to the manufacturer’s protocol for TMT kit (Thermo Fisher Scientific, Waltham, MA, USA). The tryptic peptides were fractionated by high pH reverse-phase HPLC (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... The mass to charge ratio and retention time of each identified peptide were imported into TraceFinderTM (ThermoFisher®) and the peak intensity for each peptide was obtained manually ...
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bioRxiv - Microbiology 2020Quote: ... Peptide solutions were separated from magnetic beads and further processed with HiPPR detergent removal spin columns (Thermo Scientific) and desalted by reverse phase C18 ziptip (Millipore) ...
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bioRxiv - Molecular Biology 2019Quote: Peptides have then been analyzed by liquid chromatography-mass spectrometry (LCMS) using an UltiMate 3000 LC (Thermo Scientific) coupled to either an Orbitrap Elite or a Q-Exactive mass spectrometer (Thermo Scientific) ...
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bioRxiv - Biochemistry 2021Quote: ... Peptides were injected (2.0 μL) and separated using an Ultimate 3000 RSLC nano liquid chromatography system (Thermo Scientific) coupled to a LTQ Orbitrap Velos mass spectrometer (Thermo Scientific ...
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bioRxiv - Immunology 2021Quote: ... Peptides were further purified from larger protein components by preparative HPLC on an Ultimate 3000 system (Fisher Scientific). Material was separated on a ProSwift RP-1S 4.6 x 50 mm column (Thermo Scientific ...
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bioRxiv - Cancer Biology 2019Quote: ... Tryptic peptides were separated prior to analysis using a Dionex Ultimate 3000 RSL C nanosystem (Thermo Fisher Scientific) with a C18 PepMap RSLC column (75 μm × 50 cm) ...
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bioRxiv - Microbiology 2019Quote: ... The peptides derived from in-solution digests were loaded on an EASY-nLC 1000 system (Thermo Fisher Scientific) equipped with an in-house built 20-cm column (see above) ...
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bioRxiv - Cancer Biology 2019Quote: ... The peptides were separated using a heated (55°C) 50 cm C-18 Easy-column (Thermo Fisher Scientific), and the separation was performed using an acetonitrile/water gradient (buffer A ...
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bioRxiv - Biochemistry 2019Quote: Peptides were eluted directly into the HESI source of a Thermo Orbitrap Fusion (Thermo Fisher Scientific, Waltham, MA) instrument using a low flow rate emitter ...
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bioRxiv - Genomics 2019Quote: ... and equal amounds of peptide samples were labeled with Tandem Mass Tag (TMT) 2-plex reagents (ThermoFisher Scientific) according to the manufacturer’s instructions.
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bioRxiv - Cell Biology 2020Quote: ... Peptides were reconstituted in 10 μl of H2O and reacted with 80 μg of TMT10plex (Thermo Scientific, #90111)(Werner et al. ...
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bioRxiv - Cell Biology 2021Quote: ... tryptic peptides were analyzed by NanoLC-MS/MS analyses using a LTQ Orbitrap Velos mass spectrometer (Thermo Scientific) coupled to the EASY nLC II high performance liquid chromatography system (Proxeon ...
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bioRxiv - Cell Biology 2021Quote: ... Plates were flicked and cells were incubated with 20 μl peptide diluted in HBSS buffer (Invitrogen 14065-049) complemented with 20 mM HEPES (Invitrogen 15630-056 ...
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bioRxiv - Molecular Biology 2020Quote: The tryptic peptides were analyzed in duplicate on a nano-HPLC Dionex Ultimate3000 system (Thermo Fisher Scientific, USA) coupled to a TIMS TOF Pro (Bruker Daltonics ...
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bioRxiv - Plant Biology 2021Quote: ... The eluting peptides were on-line ionized by nano-ESI deploying the Nanospray Flex Ion Source (Thermo Scientific) at 1.5 kV (liquid junction ...
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bioRxiv - Plant Biology 2020Quote: Database searching and PSM (peptide spectrum matches) processing was performed in Proteome Discoverer 2.2 (Thermo Fisher Scientific, UK). Two complementary search engines Sequest™ HT (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2021Quote: ... Peptides were identified by database searching using SEQUEST (Bioworks v3.3.1 and Proteome Discoverer V 2.4, Thermo Fisher Scientific) with a peptide tolerance of 8 ppm and a fragment tolerance of 0.1 AMU.
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bioRxiv - Microbiology 2021Quote: ... The peptides were subsequently on-line eluted into a Fusion Tribrid mass spectrometer (ThermoFisher Scientific, San Jose, CA) from an Acclaim PepMapTM RSLC C18 nano Viper analytical column (2 mm ...
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bioRxiv - Biochemistry 2021Quote: Peptides extracted from the on-bead digested samples were analyzed by the LTQ-Orbitrap mass spectrometer (Thermo Fisher) coupled with nano-LC (Dionex Ultimate 3000 ...
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bioRxiv - Microbiology 2021Quote: ... 600 ng of tryptic peptides were loaded with an UltiMate™ 3000 RSLCnano Liquid Chromatograph (Thermo Fisher Scientific) in loading solvent A (2 % acetonitrile ...