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Citations for Thermo Fisher :
3051 - 3100 of 5129 citations for MRPL54 Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... The peptide digests were frozen at −80°C and dried by vacuum centrifugation (Savant SPD121P, Thermo Fisher Scientific), reconstituted in 0.07% trifluoroacetic acid ...
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bioRxiv - Genomics 2024Quote: A vector containing the Streptavidin-Binding Peptide (SBP)-Tag was prepared in pEF1 (Thermo Fisher, Cat# V 92120), and SERBP1 ORF was cloned in frame with the His-Myc-tag present in the vector to create pSBP-SERBP1 ...
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bioRxiv - Molecular Biology 2024Quote: ... Eluates were combined prior to fractionation with the Pierce High pH Reversed-Phase Peptide Fractionation kit (Thermo Scientific). The dried TMT-labelled phosphopeptide fractions generated were resuspended in 0.1% TFA for LC-MS/MS analysis using a U3000 RSLCnano system (Thermo Scientific ...
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bioRxiv - Microbiology 2024Quote: ... Peptides were trapped on a 2 cm nanoviper Precolumn (i.d. 75 μm, C18 Acclaim PepMapTM 100, Thermo Scientific) at a flow rate of 3.0 µL/min in buffer A (2/98 MeCN/H2O in 0.1% formic acid ...
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bioRxiv - Neuroscience 2024Quote: ... Peptide concentration employed a trapping column (Acclaim C18 PepMap100, 100 μm, 2 cm, Thermo Fisher Scientific, Schwerte, Germany) with 0.1% trifluoroacetic acid (TFA ...
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bioRxiv - Microbiology 2023Quote: ... about 300 μg tryptic peptides were separated using a nanoflow DIONEX Ultimate 3000 RSLC nano System (ThermoFisher Scientific) with an XBridge Peptide BEH C18 column (300 Å ...
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bioRxiv - Cell Biology 2023Quote: Peptides were separated by nanoscale C18 reverse-phase liquid chromatography using an EASY-nLC II 1200 (Thermo Scientific) coupled to an Orbitrap Fusion Lumos mass spectrometer (Thermo Scientific) ...
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bioRxiv - Cell Biology 2023Quote: ... Peptides were trapped on a 2 cm nanoviper Precolumn (i.d. 75 μm, C18 Acclaim PepMapTM 100, Thermo Scientific) at a flow rate of 3.0 µL/min in buffer A (2/98 MeCN/H2O in 0.1% formic acid ...
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bioRxiv - Plant Biology 2024Quote: ... and peptide samples were eluted with 0.1% formic acid and analysed on an Orbitrap Elite instrument (ThermoFisher Scientific) [38] attached to an EASY-nLC 1000 liquid chromatography system (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2024Quote: ... The TMT-set was further purified using High Protein and Peptide Recovery Detergent Removal Spin Column (88305, ThermoFisher) and Pierce peptide desalting spin columns (89852 ...
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bioRxiv - Plant Biology 2023Quote: ... and peptide samples were resuspended in 0.1% FA solution and analysed on an Orbitrap Elite instrument (ThermoFisher Scientific) (Michalski et al. ...
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bioRxiv - Systems Biology 2023Quote: ... Peptides were initially separated using a Dionex UltiMate 3000 UHPLC system on an EasySpray C18 column (Thermo Scientific, OD 360 µm ...
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bioRxiv - Systems Biology 2023Quote: ... peptide digests were analyzed in DDA and DIA modes on a Thermo Scientific Eclipse mass spectrometer (Thermo Fisher) equipped with a nanoelectrospray ion source and coupled to an Easy-nLC 1200 system (Thermo Fisher) ...
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bioRxiv - Systems Biology 2023Quote: ... Proteins were digested and biotinylated peptides were captured (80 μl Pierce™ Streptavidin UltraLink™ Resin, ThermoFisher Scientific), resin was washed ...
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bioRxiv - Biochemistry 2022Quote: ... The peptides were identified by ESI mass spectrometry (MS) performed on an LCQ Fleet Ion Trap (Thermo Scientific) mass spectrometer ...
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bioRxiv - Microbiology 2023Quote: ... Peptides were analysed by on-line nanoflow LC using the Ultimate 3000 nano system (Dionex/Thermo Fisher Scientific). Samples were loaded onto a trap column (Acclaim PepMap 100 ...
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bioRxiv - Microbiology 2022Quote: ... was synthesized as a custom peptide with N-terminal acetylation and C-terminal amidation (Life Technologies Europe Bv).
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bioRxiv - Molecular Biology 2023Quote: ... The peptides mixtures were analyzed by nano-LC-MS/MS using an Ultimate 3000 system (Thermo Fisher Scientific) coupled to an LTQ-Orbitrap Velos mass spectrometer ...
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bioRxiv - Molecular Biology 2023Quote: All peptide samples were separated with an online nanoflow Proxeon EASY-nLC 1200 UHPLC system (Thermo Fisher Scientific) and analyzed on an Orbitrap Exploris 480 mass spectrometer (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2023Quote: ... Peptides were analysed by on-line nanoflow LC using the Ultimate 3000 nano system (Dionex/Thermo Fisher Scientific). Samples were loaded onto a trap column (Acclaim PepMap 100 ...
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bioRxiv - Neuroscience 2023Quote: ... Peptide concentrations in mass spectrometry ready samples were measured using the mBCA assay (Thermo Scientific™ Pierce™). Three stable isotope labeled reference peptides were spiked into the final peptide samples at known concentrations (Vivitide ...
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bioRxiv - Molecular Biology 2023Quote: ... Separation of peptides by reversed-phase chromatography was carried out on an UltiMate 3000 RSLCnano System (Thermo Scientific) which was equipped with a C18 Acclaim Pepmap100 preconcentration column (100µm i.D.x20mm ...
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bioRxiv - Biochemistry 2023Quote: ... Peptides were reconstituted in 10 µl of H2O and reacted with 80 µg of TMT10plex (Thermo Scientific, #90111) label reagent dissolved in 4 µl of acetonitrile for 1 h at room temperature ...
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bioRxiv - Bioengineering 2023Quote: ... The purified peptide solution was dried and quantified using the Micro BCA Protein Assay Kit (Thermo Fisher Scientific). An equal amount of peptide (∼50 μg ...
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bioRxiv - Cancer Biology 2023Quote: ... Labeled peptides were fractionated via highpH reverse-phase high-performance liquid chromatography (Thermo Easy nLC1000; Thermo Fisher Scientific) using a precolumn (made in house ...
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bioRxiv - Molecular Biology 2023Quote: LC-MS/MS analysis 500 ng of peptides was performed on a Orbitrap Exploris 480 (Thermo Fisher Scientific) equipped with a nano-electrospray ion source and FAIMS (CV50 ...
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bioRxiv - Molecular Biology 2023Quote: The resulting peptides were subsequently labelled using TMT-11plex Isobaric Mass Tagging Kit (Thermo Scientific, Rockford, IL, USA) according to the manufacturer’s instructions as indicated in supplementary materials and methods ...
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bioRxiv - Microbiology 2023Quote: ... Equal amounts of desalted peptides were labelled immediately after being quantified with Qubit protein assay (Thermo Fisher Scientific) as per manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: The digested peptides were separated by online reversed phase chromatography on an Dionex UltiMate 3000 (Thermo Fisher Scientific) (column packed with Poroshell 120 EC C18 ...
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bioRxiv - Systems Biology 2023Quote: Peptides were labeled by addition of a third of an aliquot of TMT11-131C label reagent (Thermo Scientific) in a final volume of 20% acetonitrile and incubated for 1 hour at 600 rpm and 25°C ...
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bioRxiv - Microbiology 2023Quote: ... Online detection of peptide ion was by electrospray ionization mass spectrometry using an Orbitrap Elite MS (Thermo Scientific). Peptides were separated on a PepmapTM C18 reversed phase column (3 μm ...
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bioRxiv - Microbiology 2023Quote: ... The combined sample was desalted using a Pierce peptide desalting spin column (Thermo Fisher Scientific; catalog no. 89852) and vacuum-dried in a Centrivap (Labconco ...
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bioRxiv - Microbiology 2023Quote: ... Mass spectrometric analysis of eluting peptides was conducted on an Orbitrap Exploris 480 (Thermo Fisher Scientific; Waltham, US) instrument platform ...
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bioRxiv - Developmental Biology 2023Quote: Raw mass spectrometry data was analyzed for peptide identification and quantification with Proteome Discoverer 2.5 (Thermo Fisher Scientific), using the SequestHT search engine against the Homo sapiens UniProt database (UP000005640 ...
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bioRxiv - Systems Biology 2023Quote: Tryptic peptides were separated by reverse phase chromatography on a Dionex Ultimate 3000 RSLCnano UHPLC system (Thermo Scientific) with an Acclaim C18 PepMap RSLC column using a 3-42% acetonitrile gradient over 60 minutes ...
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bioRxiv - Biochemistry 2023Quote: ... Peptides were loaded and concentrated on PepMap C18 precolumns (length, 5 mm; inner diameter, 0.3 mm; Thermo Scientific) at a flow rate of 30 µl/min and separated using Acclaim PepMap C18 reversed-phase nano-LC columns (length ...
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bioRxiv - Biochemistry 2022Quote: Lyophilized peptide samples were suspended in 0.25X PBS buffer and concentrations were determined using a Nanodrop 2000 (ThermoFisher). CD spectra were collected using a JASCO J-815 CD spectrometer sampling every 0.5 nM between 260 nM and 180 nM ...
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bioRxiv - Biochemistry 2023Quote: ... Peptides were separated on a 50 cm 75 μm inner diameter EasySpray ES903 microcapillary column (Thermo Fisher Scientific). Peptides were separated over a 190 min gradient of 6 - 27% acetonitrile in 1.0% formic acid with a flow rate of 300 nL/min.
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bioRxiv - Cancer Biology 2023Quote: ... of the mRNA solution was mixed with 5μl of 20mM p5RHH peptide solution and 187μl of 1x HBSS (Gibco) to prepare the nanoparticle complex and immediately injected into the mouse through the tail vein21 ...
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bioRxiv - Cell Biology 2023Quote: ... Peptides were collected by centrifugation and labelled using TMT 10-plex isobaric mass tagging reagents (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... four micrograms of desalted peptides were labeled with tandem mass tags (TMT10plex, Thermo Fisher Scientific cat. No 90110) using a 1:20 ratio of peptides to TMT reagent ...
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bioRxiv - Cell Biology 2023Quote: ... Analysis of the eluted tryptic peptides was performed online using a Q Exactive Plus mass spectrometer (Thermo Scientific) possessing a Nanospray Flex Ion source (Thermo Fisher ...
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bioRxiv - Biochemistry 2023Quote: ... The separated peptides were electrosprayed in the positive ion mode into an Orbitrap Eclipse Tribrid mass spectrometer (ThermoFisher) operated in data-dependent acquisition mode (3 s cycle time ...
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bioRxiv - Biochemistry 2023Quote: ... Peptides were diluted to 60 µL with LC/MS-grade water and labeled using TMTpro labels (Thermo Scientific) for 1 h at room temperature ...
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bioRxiv - Immunology 2023Quote: ... Peptides were separated across a 2.1mm X 5cm C18 separating column (1.9 um Hypersil Gold, Thermo Fisher Scientific) with a linear gradient of 4% - 40% CH3CN and 0.3% formic acid ...
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bioRxiv - Neuroscience 2023Quote: ... 20 μg peptide from each sample was labeled with TMT10plex Isobaric Label Reagent Set (ThermoFisher Scientific, cat# A37725) following to the manufacture’ s instruction ...
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bioRxiv - Molecular Biology 2023Quote: ... Peptides were trapped on a μPrecolumn C18 PepMap100 (5μm, 100 Å, 300 μm × 5mm, ThermoFisher Scientific, Reinach, Switzerland) and separated by backflush on a C18 column (5 μm ...
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bioRxiv - Immunology 2023Quote: ... PCSK9 peptides were conjugated to Qß bacteriophage VLPs using succinimidyl 6-((ß-maleimidopropionamido)hexanoate) (SMPH; Thermo Fisher Scientific), as described previously60 ...
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bioRxiv - Cell Biology 2023Quote: ... Peptides were separated on a 50 cm (2 µm particle size) EASY-Spray column (Thermo Fisher Scientific, UK), which was assembled on an EASY-Spray source (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: Peptides were fractionated using high-pH liquid-chromatography on a micro-flow HPLC (Dionex U3000 RSLC, Thermo Scientific). 45µg of pooled and purified TMT labelled peptides resuspended in Solvent A (5mM ammonium-bicarbonate ...