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Citations for Thermo Fisher :
3101 - 3150 of 10000+ citations for Human UFL1 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... Cells were transfected with appropriate plasmids using Lipofectamine 3000 (Thermo Fisher) by reverse transfection as previously described (30) ...
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bioRxiv - Systems Biology 2023Quote: ... One μg of sgRNA plasmids were transfected with Lipofectamine 3000 (Invitrogen) 1 μg of pPAX2 and 0.1 μg VSVG into HEK293Ts seeded the night before at 2e5 cells per 6 well ...
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bioRxiv - Developmental Biology 2023Quote: ... 4 μg of the plasmid was transfected using Lipofectamine 2000 (Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2023Quote: ... and cloned into the pENT-D-Topo plasmid (Thermofisher, Rochester,U.S.A). The entry vectors were fully sequenced to ascertain the integrity of the cloned sequences and were used to transfer the branching enzyme sequences to the ChampionTM pET300 plasmid (Thermofisher ...
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bioRxiv - Microbiology 2023Quote: ... which were purified with GeneJET Plasmid Midiprep kit (Thermo Scientific, USA) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were transfected with plasmid DNA using Lipofectamine 2000 (ThermoFisher #11668019) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... and transfer plasmid pLenti-NLuc1-9_IRES_EGFP (Figure S5) using Lipofectamine2000 (Invitrogen). DNA ratio of plasmid mix was 2:1:4 ...
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bioRxiv - Cell Biology 2023Quote: ... Plasmids were purified using a maxiprep kit (Thermo Fisher Scientific, A31217).
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bioRxiv - Cell Biology 2023Quote: Plasmid DNA (1-2 μg) was transiently transfected with Optimem (Gibco) and Lipofectamine 2000 (ThermoFisher ...
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bioRxiv - Molecular Biology 2023Quote: ... plasmids at a 49:1 ratio using Lipofectamine 2000 (ThermoFisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: Plasmids were transfected using TurboFect transfection reagent (ThermoFisher Scientific, Basel, Switzerland) and siRNAs using Lipofectamine RNAiMax (ThermoFisher Scientific ...
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bioRxiv - Biophysics 2023Quote: ... modified pFastBac1 plasmids was performed using T4 DNA Ligase (Thermo Scientific). Ligated plasmids were amplified in E ...
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bioRxiv - Neuroscience 2023Quote: ... The plasmid was used to transfect Expi293F cells (Thermo Fisher, USA) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... 1 µg plasmid DNA was diluted in 100 µl OptiMEM (ThermoFisher). The DNA mix was incubated with 100 µl OptiMEM containing 3 µl Lipofectamine2000 ...
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bioRxiv - Microbiology 2023Quote: ... 2 µg plasmid DNA was diluted in 100 µl OptiMEM (ThermoFisher). The DNA mix was incubated with 100 µl OptiMEM containing 6 µl polyethylenimine (PEI ...
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bioRxiv - Cell Biology 2023Quote: ... cells were transfected with the respective plasmids using Lipofectamine 2000 (Invitrogen) following the instructions provided by the manufacturer ...
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bioRxiv - Molecular Biology 2023Quote: ... plasmids at a 49:1 ratio using Lipofectamine 2000 (ThermoFisher Scientific) and read out as described above 24 h later.
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bioRxiv - Neuroscience 2023Quote: ... and the indicated plasmids were co-transfected using Lipofectamine 3000 (Invitrogen) following the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: Plasmids were mainly constructed based on the Gateway technology (Life technologies). Primers used in the plasmid construction are listed in Table S1 ...
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bioRxiv - Cell Biology 2023Quote: Transient transfection with plasmids was done either using Lipofectamine 2000 (Invitrogen) or Xfect (Takara ...
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bioRxiv - Cell Biology 2023Quote: ... followed by addition of plasmid DNA in conjunction with lipofectamine2000 (Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... 500ng of plasmid and 2uL of lipofectamine 2000 (Invitrogen, Cat #11668019) were each diluted in 50uL Opti-MEM (Gibco ...
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bioRxiv - Microbiology 2023Quote: ... or the PureLink HiPure Plasmid Midiprep kit (catalog number K210005; Invitrogen).
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bioRxiv - Cell Biology 2023Quote: ... plasmid (1.5 µg) was overexpressed using Lipofectamine 2000 (Invitrogen, 11668-019). After 24 hours post transfection ...
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bioRxiv - Cell Biology 2023Quote: ... transfection of plasmid vectors was carried out using Lipofectamine 2000 (Invitrogen) following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... the plasmid was digested with BsmBI (FastDigest Esp3I, Thermo Fisher Scientific) and a synthetic 391 bp double-stranded DNA fragment encoding 5′-(1st gRNA/scaffold/H1 promoter/2nd gRNA)-3′ was inserted using the NEBuilder HiFi assembly system (NEB) ...
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bioRxiv - Evolutionary Biology 2022Quote: ... DNA was purified using the GeneJet Plasmid Miniprep Kit (Thermo Scientific) and verified by full-length plasmid sequencing (Plasmidsaurus) ...
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bioRxiv - Biochemistry 2023Quote: ... Concentration of all plasmids was measured by NanoDrop One (Thermo Scientific) and the plasmids were stored at −80°C.
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bioRxiv - Biochemistry 2023Quote: ... 4 μg plasmids were mixed with 8 μL Lipofectamine 2000 (Invitrogen) and the mixture was added to cells followed by incubation at 37 °C for at least 24 h ...
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bioRxiv - Immunology 2023Quote: ... All plasmids were transfected using Lipofectamine 2000 (11668019, Thermo Fisher Scientific) following manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... Plasmids were transiently transfected using Lipofectamine 3000 Transfection Reagent (Thermo Scientific) according to the protocol provided by the manufacturer ...
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bioRxiv - Biochemistry 2022Quote: ... The sen cDNA was ligated into plasmid blunt II Topo (Invitrogen) and then transformed into TOP10 competent Escherichia coli cells ...
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bioRxiv - Cell Biology 2023Quote: ... Plasmid concentration was measured by a NanoDrop Spectrophotometer (Thermo Fisher Scientific).
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bioRxiv - Cancer Biology 2023Quote: ... and 30.4 μg of plasmid DNA diluted in Opti-MEM (Gibco). Media was replaced with fresh culture medium after 7 h and cells were harvested 48 h post-transfection.
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bioRxiv - Cell Biology 2023Quote: ... cells were transfected with the corresponding plasmid using Lipofectamine 2000 (Invitrogen) following the manufacture recommendations and selected with 300 ug/mL of geneticin for 10-14 days ...
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bioRxiv - Cell Biology 2022Quote: ... Plasmid transient transfection was performed using Lipofectamine 3000 (Invitrogen, Carlsbad, CA) and siRNA transfection was conducted using Lipofectamine RNAiMAX (Invitrogen ...
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bioRxiv - Biophysics 2023Quote: ... All transient plasmid DNA transfections were done using Lipofectamine 2000 (Invitrogen) following the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... adenoviral plasmids were transfected into 293A cells using Lipofectamine 2000 (Invitrogen). 293A cells were cultured in DMEM (Sigma 5796) ...
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bioRxiv - Cell Biology 2022Quote: ... The miRNA plasmid was transfected using Lipofectamine LTX (Thermo Fisher Scientific) as described in manufacture’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... Purified ligated plasmids were electroporated into MegaX DH10B (Thermo Fisher Scientific) and plated on LB/Amp plates ...
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bioRxiv - Developmental Biology 2023Quote: ... The entire construct was cloned into a pJet1.2 plasmid backbone (ThermoFisher) using Gibson assembly master mix (NEB ...
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bioRxiv - Biochemistry 2023Quote: ... The plasmid constructs were transfected using Lipofectamine® 2000 Reagent (Invitrogen) as per the manufacturer’s instructions72h after transfection 1.2mg/ml of G-418 (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2023Quote: ... Positive colonies containing pYES2/NT C plasmids (Thermo Fisher, Cat#: V825220), expressing N-terminal His-tagged CSLD2 ...
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bioRxiv - Developmental Biology 2023Quote: ... at a ratio of 1:1.5 plasmids: lipofectamine 3000 (Invitrogen, L3000001) overnight ...
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bioRxiv - Cancer Biology 2023Quote: ... and prepared using the PureLinkTM Quick Plasmid Miniprep Kit (Invitrogen, #K210010). Plasmid identification was verified via Sanger sequencing.
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bioRxiv - Immunology 2023Quote: ... and CAR plasmids were transfected into HEK293T host cells (in Gibco DMEM HG medium containing 10% FBS ...
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ER mediates spatial regulation of lysosome-endosome interactions via motion switch at junction sitesbioRxiv - Cell Biology 2023Quote: ... Transfection of plasmid DNA was performed using Lipofectamine 3000 (Invitrogen L3000015) according to the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2023Quote: Plasmid pJK14-Tn4rev was designed using Vector NTI software (Life Technologies) and synthesized de novo by GENEWIZ Gene Synthesis Services (GENEWIZ ...
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bioRxiv - Molecular Biology 2023Quote: ... All plasmid transfections were carried out using Lipofectamine 2000 (Thermo Fisher) according to manufacturer’s instructions.
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bioRxiv - Plant Biology 2024Quote: ... and transferred into Ti plasmids by multisite Gateway LR reaction (Thermofisher) for simultaneous expression of right and left platinum TALENs fused to the Arabidopsis delta prime subunit mitochondrial presequence ...