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Citations for Thermo Fisher :
3151 - 3200 of 10000+ citations for Human UFL1 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... transfection of plasmid vectors was carried out using Lipofectamine 2000 (Invitrogen) following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... plasmids at a 49:1 ratio using Lipofectamine 2000 (ThermoFisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... the plasmid was digested with BsmBI (FastDigest Esp3I, Thermo Fisher Scientific) and a synthetic 391 bp double-stranded DNA fragment encoding 5′-(1st gRNA/scaffold/H1 promoter/2nd gRNA)-3′ was inserted using the NEBuilder HiFi assembly system (NEB) ...
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bioRxiv - Molecular Biology 2023Quote: ... All plasmid transfections were carried out using Lipofectamine 2000 (Thermo Fisher) according to manufacturer’s instructions.
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ER mediates spatial regulation of lysosome-endosome interactions via motion switch at junction sitesbioRxiv - Cell Biology 2023Quote: ... Transfection of plasmid DNA was performed using Lipofectamine 3000 (Invitrogen L3000015) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... Purified PCR products were then cloned into the pDONR221 plasmid (Invitrogen). For CpomIR41a1 ...
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bioRxiv - Systems Biology 2023Quote: ... One μg of sgRNA plasmids were transfected with Lipofectamine 3000 (Invitrogen) 1 μg of pPAX2 and 0.1 μg VSVG into HEK293Ts seeded the night before at 2e5 cells per 6 well ...
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bioRxiv - Molecular Biology 2023Quote: ... contaminating genomic or plasmid DNA was removed using ezDNase (Invitrogen 11766050), followed by reverse transcription using SuperScript IV VILO master mix (Invitrogen 11766050) ...
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bioRxiv - Synthetic Biology 2023Quote: Plasmid pJK14-Tn4rev was designed using Vector NTI software (Life Technologies) and synthesized de novo by GENEWIZ Gene Synthesis Services (GENEWIZ ...
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bioRxiv - Microbiology 2023Quote: ... codon-optimized spike expression plasmid using Lipofectamine 2000 (Invitrogen™ 11668019) following manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: All the plasmids and siRNAs were transfected using Lipofectamine 2000 (Invitrogen) and RNAiMAX (Invitrogen ...
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bioRxiv - Microbiology 2023Quote: ... faecalis and the PureLink® Quick Plasmid Miniprep Kit (Invitrogen, USA) to isolate plasmid DNA from E ...
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bioRxiv - Microbiology 2023Quote: ... plasmid DNA and Expifectamine were mixed in Opti-PRO SFM (Gibco) according to the manufacturer’s instructions and added to the cells ...
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bioRxiv - Microbiology 2023Quote: ... pET_AV101_TSP1-4 extracted with GeneJET Plasmid Miniprep Kit (Thermo Scientific™). Vectors carrying the correct tsp insert (pET_AV101_TSP1-4 ...
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bioRxiv - Microbiology 2023Quote: ... the GeneJET Plasmid Miniprep Kit (Thermo Fisher Scientific GmbH, Schwerte, Germany) was used to purify the plasmid DNA after cloning the PCR products using the CloneJET™ PCR Cloning Kit with DH10B Competent Cells (Thermo Fisher Scientific GmbH ...
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bioRxiv - Microbiology 2023Quote: ... This was purified using the GeneJET plasmid miniprep kit (Thermo Fisher), and linearised using NdeI (NEB ...
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bioRxiv - Developmental Biology 2023Quote: ... 4 μg of the plasmid was transfected using Lipofectamine 2000 (Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2023Quote: ... ExpiFectamine reagent and plasmid DNA were diluted into OPTIMEM (Gibco™) according to the amount determined by the manufacturer’s transfection protocol ...
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bioRxiv - Biochemistry 2023Quote: ... plasmids were transfected into cell culture using lipofectamine 2000 (Invitrogen, 11668019) following the manufacturer’s instruction ...
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bioRxiv - Cancer Biology 2023Quote: ... Plasmids were transfected with Lipofectamine™ 3000 Transfection Reagent (Invitrogen; # L3000015), according to the manufacturer’s instructions.
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bioRxiv - Cell Biology 2023Quote: ... Plasmids were purified using a maxiprep kit (Thermo Fisher Scientific, A31217).
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bioRxiv - Neuroscience 2023Quote: Primary neurons were transfected with expression plasmids using Lipofectamine 2000 (Invitrogen). In brief ...
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bioRxiv - Synthetic Biology 2024Quote: ... Colonies were picked and plasmids extracted using miniprep (Thermo Fisher #K0503). After sequencing of ubiquitin barcode and protospacer region ...
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bioRxiv - Genetics 2024Quote: ... The plasmids were transfected into HCT116 cells using Lipofectamine 3000 (Invitrogen). For POLQ KO in HF1 fibroblasts ...
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bioRxiv - Biochemistry 2024Quote: ... plasmids were either bought or generated using the Gateway Technology (Invitrogen). Three 10 cm dishes of HEK293T (4 million cells/dish ...
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bioRxiv - Cancer Biology 2024Quote: ... HOXC8 and HOXC9 plasmids were transfected using Lipofectamine 3000 reagent (ThermoFisher), following the manufacturer’s guidelines ...
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bioRxiv - Cell Biology 2024Quote: ... These plasmids were transfected in C2C7 myoblasts with TurboFect (ThermoFisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2024Quote: ... Cells were transfected with pX330 plasmid (2.5µg) using Lipofectamine 3000 (Invitrogen) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were transfected with plasmids using Lipofectamine 2000 Reagent (Life Technologies) and Opti-MEM according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... Each plasmid was mixed with 9 μL of Lipofectamine2000 (Invitrogen, UA) and incubated for 20 minutes at room temperature before being transferred into the growth medium ...
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bioRxiv - Genomics 2024Quote: ... plasmids using the neon or neon nxt transfection systems (Life Technologies). Forty-eight hours after transfection ...
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bioRxiv - Cell Biology 2024Quote: Cells were transfected with plasmids using Lipofectamine 2000 Reagent (Life Technologies) and Opti-MEM according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... pRL3- and pFFL plasmids (0.25 μg each) using Lipofectamine 2000 (Invitrogen). Cell extracts were prepared at 16 or 24 hpi for luciferase activity measurement and western blot.
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bioRxiv - Biochemistry 2024Quote: Expression plasmids were transformed into chemically competent BL21(DE3) cells (Invitrogen). For Intein Donor constructs with pAzF incorporated via GCE ...
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bioRxiv - Microbiology 2024Quote: ... Recombinant plasmids were transfected into HEK293T cells using Lipofectamine 2000 (Invitrogen) together with lentiviral packaging vectors pMD2.G and psPAX2 (gifts from Didier Trono ...
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Antibodies targeting Crimean-Congo hemorrhagic fever virus GP38 limit vascular leak and viral spreadbioRxiv - Microbiology 2024Quote: ... Both plasmids were transiently transfected into FreeStyle 293-F cells (Invitrogen) using polyethyleneimine ...
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bioRxiv - Cell Biology 2024Quote: ... Plasmids ligated using the Rapid DNA Ligation Kit (Thermo Scientific, K1422) as per manufacturer’s instructions ...
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bioRxiv - Genetics 2024Quote: ... Plasmids were amplified in DH5-alpha competent cells (EC0112, Thermo Fisher), isolated using a kit (M+N Nucleospin Plasmid kit) ...
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bioRxiv - Immunology 2024Quote: ... The lentiviral CRISPR plasmids were digested with BsmBI (Thermo Fisher Scientific) for 30 min at 37°C ...
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bioRxiv - Synthetic Biology 2024Quote: ... 4.5 µg of plasmid quantified by Qubit dsDNA broad range (Invitrogen) was used to transform 107 A ...
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bioRxiv - Cancer Biology 2021Quote: Human melanocytes and melanoma cells were lysed in Pierce RIPA buffer (Thermo Scientific) containing 1× Halt protease inhibitor cocktail (100× ...
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bioRxiv - Cancer Biology 2021Quote: ... Human CRC organoids medium is composed of Ad-DF+++ (Advanced DMEM/F12 (GIBCO) supplemented with 2 mM Ultraglutamine I (Lonza) ...
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bioRxiv - Cell Biology 2020Quote: Primary human umbilical vein endothelial cells (HUVECs) were obtained from Gibco (Cat#: C0035C) and cultured in Medium 200 (Cat# ...
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bioRxiv - Cell Biology 2020Quote: ... HIF1A levels were measured in duplicates applying a HIF1A Human ELISA kit (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... plate was incubated with HRP conjugated anti-human IgA (1:1000, Thermo Scientific) for 1hour in TBST with 1% non-fat dry milk ...
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bioRxiv - Cell Biology 2020Quote: Human induced pluripotent stem cells (hiPSCs) were cultured in StemFlex media (Gibco, #A3349401) and passaged with EDTA (Life Technologies #15575-020) ...
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bioRxiv - Immunology 2021Quote: ... HRP conjugated Rabbit anti-Human IgG(H+L) (Cat. No. A18903, Invitrogen, USA) as added to each well as the detection antibody ...
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bioRxiv - Genetics 2019Quote: ... RPE1 cells and primary human fibroblasts were cultivated in complete DMEM medium (Gibco).
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bioRxiv - Molecular Biology 2021Quote: Human MSCs were maintained in growth media (Dulbecco’s Modified Eagle Medium (DMEM, Gibco) containing D-glucose (1g/L) ...
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bioRxiv - Molecular Biology 2021Quote: ... supplemented with human keratinocyte growth supplement (catalogue #S-001-5, Thermo Fisher Scientific) and 1% penicillin/streptomycin (catalogue #15140-122 ...