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Citations for Thermo Fisher :
2901 - 2950 of 10000+ citations for Human UFL1 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... A549 human lung epithelial cells (ATCC) were cultured in F12K media (Gibco) supplemented with 10% FBS ...
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bioRxiv - Molecular Biology 2023Quote: ... SW13 human adrenocortical carcinoma cells were cultured in high glucose DMEM (Invitrogen) supplemented with 10% fetal calf serum and antibiotics ...
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bioRxiv - Biophysics 2023Quote: ... incubated with horseradish peroxidase-conjugated anti-human IgG (InvitrogenTM, ThermoFisher, Waltham, MA) and anti-mouse IgG (ThermoFisher ...
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bioRxiv - Cancer Biology 2023Quote: ... bovine pituitary extract and human recombinant epidermal growth factor (Gibco, 37000-015)) and primary gingival keratinocyte (Dermal cell basal medium (ATCC ...
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bioRxiv - Cell Biology 2023Quote: ... hiPSCs were cultured on human recombinant vitronectin in StemFlexTM media (Life Technologies) and differentiation was initiated by plating singularised hiPSCs on human embryonic stem cells (hESC)-qualified Matrigel (Corning ...
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bioRxiv - Cell Biology 2023Quote: ... and/or Alexa Fluor 647-conjugated human transferrin (25 μg/ml; Invitrogen) were added to the cells for some experiments.
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bioRxiv - Bioengineering 2023Quote: ... or anti-human CD68 (IgG3, kappa, PG-M1, ThermoFisher Scientific, MA, USA) antibodies were diluted 1:50 in PBS supplemented with 0.1% BSA and applied on the devices overnight at 4 °C ...
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bioRxiv - Immunology 2023Quote: ... Recombinant human IFN-β (1000 U/ml to 1 U/ml, ThermoFisher) was used as positive controls for IRF activation ...
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bioRxiv - Microbiology 2023Quote: ... human MARCH4 and mouse MARCH2 (mMARCH2) into pcDNA3.1/myc-His A (Invitrogen) and pBJ5 vector have been previously described (10) ...
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bioRxiv - Biochemistry 2024Quote: ... CCR6+ T cells were activated with Dynabeads Human T-activator (Gibco, 11132D) at a density of 1 × 106 cell/ml in growth media (RPMI1640 media with 10% serum ...
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bioRxiv - Immunology 2023Quote: Human HEK293T cells were grown in Dulbecco’s modified Eagle’s medium (DMEM, Gibco) supplemented with 10 % fetal bovine serum (FBS ...
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bioRxiv - Microbiology 2023Quote: ... Leptin levels were determined using Human Leptin Instant ELISA kit (Invitrogen BMS2039INST) according to manufacturer’s instructions.
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bioRxiv - Cell Biology 2023Quote: ... and Alexa Fluor594-conjugate human transferrin (T13343) were obtained from Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2024Quote: ... human or murine aortic SMCs were transfected with Lipofectamine 2000 (Life Technologies) containing Scr RNA or siRNA targeting ELN ...
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bioRxiv - Genomics 2024Quote: ... Human GPR108 (NCBI RefSeq NP_001073921) was synthesized as GeneArt Strings by Invitrogen and cloned into pSBbi using Gibson cloning.
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bioRxiv - Microbiology 2024Quote: ... PE-coupled mouse anti-Human CD86 (Invitrogen, 12-0869-42; 1/100), APC-coupled mouse anti-Human CD83 (Miltenyi Biotec ...
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bioRxiv - Neuroscience 2024Quote: Human iPSCs from the WTC11 background were cultured in StemFlex Medium (GIBCO/Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2024Quote: ... 9.7 ng/mL human EGF recombinant protein (Thermo Fisher Scientific, cat. PHG0313), 23.4 µg/mL adenine (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2024Quote: ... or human siCIC (Horizon L-015185-01-0020) using Lipofectamine RNAiMAX (Invitrogen). Differentiation media was then changed two and four days after transfection ...
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bioRxiv - Cancer Biology 2024Quote: ... and mouse anti-human CD45RO PE-Cy7 (Invitrogen Cat 25-0457-42). Flow cytometric data were analyzed using SpectroFlo (Cytek ...
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bioRxiv - Cell Biology 2024Quote: ... the medium was further changed to Human Endothelial SFM (Thermo Fisher Scientific) supplemented with 5% Knockout serum replacement (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2024Quote: ... goat anti-human H+L conjugated Alexa 488 (Thermo Fisher Scientific A11013) as the secondary antibody ...
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bioRxiv - Immunology 2024Quote: ... human iPSCs were induced to undergo definitive endoderm differentiation using RMPI1640 (Gibco) supplemented with Activin A (100 ng/ml ...
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bioRxiv - Molecular Biology 2024Quote: ... incubated with a rabbit anti-human CRIP1 primary antibody (ThermoFisher, #PA5-24643). Cells were then incubated with an Alexa-647 anti-rabbit secondary antibody and washed with flow buffer ...
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bioRxiv - Cell Biology 2024Quote: ... supplemented with 5 ng/mL of human epidermal growth factor (hEGF, ThermoFisher), 20 ng/mL of nerve growth factor (NGF ...
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bioRxiv - Systems Biology 2024Quote: ... mouse anti-human Tau monoclonal antibody-HT7(1:2000; Thermo Fisher # MN1000); mouse anti-S396/S404 phospho-Tau monoclonal antibody-PHF1 (1:500 ...
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bioRxiv - Bioengineering 2020Quote: ... and formed into plasmids with T4 DNA ligase (Thermo Fisher Scientific) following the user guide for self-circularization of linear DNA ...
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bioRxiv - Synthetic Biology 2021Quote: ... Appropriate plasmids were transfected into the cells using Lipofectamine 2000 (Invitrogen) according to the manufacturer’s protocols ...
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bioRxiv - Synthetic Biology 2021Quote: ... Plasmids were transformed into BL21(DE3) competent cells (Thermo Fisher Scientific). Protein expression was conducted in LB medium supplemented with kanamycin ...
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bioRxiv - Cancer Biology 2021Quote: ... Plasmids were transfected into cells using Lipofectamine 3000 (Thermo Fisher, USA) following the manufacturers’ instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... Plasmids for each targeting pair were co-transfected using Turbofectin (Thermofisher). After 48 hours cells were put under selection with 1 ug/mL puromycin ...
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bioRxiv - Cell Biology 2020Quote: ... Transfections with plasmid DNA were performed using Lipofectamine2000 reagent (Thermo Fisher) according to manufacturer’s recommendation.
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bioRxiv - Developmental Biology 2021Quote: ... mESCs were transfected with 5 µg plasmid using Lipofectamine 2000 (Invitrogen) and puromycin selected (1 µg/ml) ...
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bioRxiv - Immunology 2022Quote: ... cloned plasmids (350 μg) were transfected into HEK293F cells (Life Technologies) (1 million cells/mL ...
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bioRxiv - Molecular Biology 2021Quote: ... Plasmid DNA was prepared using Stbl3 competent cells (#C7373-03, Invitrogen) and Qiagen midi prep kits (#12143).
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bioRxiv - Molecular Biology 2020Quote: ... protein-coding sequences were subcloned into the pAc5.1 plasmid (Life Technologies). Different full-sized variants of Pita were fused with 3xFLAG and cloned into an expression vector ...
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bioRxiv - Molecular Biology 2021Quote: ... protein-coding sequences were subcloned into the pAc5.1 plasmid (Life Technologies). Details of the cloning procedures ...
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bioRxiv - Cell Biology 2022Quote: ... and plasmid mixture for packaging (ViraPower HiPerform Lentiviral Expression Systems, Invitrogen) were transfected into 293 package cells with Lipofectamine 2000 (Invitrogen ...
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bioRxiv - Cell Biology 2022Quote: ... neurons were transfected with plasmids using Lipofectamine LTX (Invitrogen, Carlsbad, CA).
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bioRxiv - Cell Biology 2022Quote: MEFs were transfected with appropriate plasmids using Lipofectamine 3000 (Thermo Fisher) by reverse transfection ...
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bioRxiv - Genomics 2020Quote: ... and transfer plasmid (LentiCRISPRv2) were mixed with Lipofectamine® 2000 (ThermoFisher) in OptiMEM (ThermoFisher ...
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bioRxiv - Cancer Biology 2019Quote: ... TALEN plasmids were linearized and transcribed with mMESSAGE mMACHINE kit (Ambion). Zebrafish embryos were injected with 50 pg of mRNA of each TALEN arm ...
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bioRxiv - Cancer Biology 2019Quote: ... Cells were transfected with pSpCas9-sgSTK11 plasmid using LipofectAMINE 2000 (Invitrogen) according to manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2019Quote: ... the selected pool was cloned into a TOPO TA plasmid (Invitrogen) and transformed into DH5α E ...
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bioRxiv - Developmental Biology 2019Quote: ... Plasmid transfections were performed using Lipofectamine 2000 (Thermo Scientific®; 11668019)
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bioRxiv - Cell Biology 2019Quote: ... or mito-PAGFP plasmids using Lipofectamine® 2000 (Life Technologies #11668), as per the manufacturer’s protocol ...
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Parallelized engineering of mutational models using piggyBac transposon delivery of CRISPR librariesbioRxiv - Bioengineering 2020Quote: ... cells were transfected with PB plasmids using Lipofectamine Stem reagent (Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2019Quote: ... Plasmids were transfected using Lipofectamine 2000 DNA transfection reagent (ThermoFisher Scientific). Pooled and clonal cell lines were selected by limiting dilution in neomycin (G418 ...
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bioRxiv - Cell Biology 2019Quote: ... The linearised plasmid was purified (GeneJET Gel Extraction Kit, ThermoFisher Scientific), and co-transfected into RPE1 Cyclin B1-Venus:Ruby-MAD2 (Collin et al. ...
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bioRxiv - Cell Biology 2019Quote: ... PyronicSF was targeted to mitochondria or nucleus using pSHOOTER plasmids (ThermoFisher). PyronicSF plasmids are available from Addgene.