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Citations for Thermo Fisher :
3101 - 3150 of 10000+ citations for 6H 1 3 Dioxolo 4 5 g 1 benzopyran 6 one 7 8 dihydro since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... p21) and pAAV target plasmid in a 4:1:1:2 molar ratio by use of Lipofectamine 2000 (ThermoFisher) according to company protocol ...
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bioRxiv - Cell Biology 2023Quote: ... The relative expression of target cDNAs within the sample set was calculated from a serial dilution (1:4–1:256) (standard curve) of a cDNA pool using StepOneTM software V2.0.2 (Applied Biosystems). Real-time PCR data were then normalized to elongation factor-1 alpha (eF1α ...
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bioRxiv - Bioengineering 2024Quote: ... Plasma samples were thawed from -80°C storage to room temperature and diluted 1:4 with 1× PBS (Gibco). Samples were then transferred to a 96-well clear bottom black plate (Thermo Scientific ...
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bioRxiv - Developmental Biology 2023Quote: ... Cells were passaged with 80-90% confluence at a split ratio of 1:2-1:4 using TrypLE (Gibco) for 8 min at 37°C ...
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bioRxiv - Systems Biology 2023Quote: ... A 1 mL aliquot of 2:2:1 (v/v) mixture of ≥ 99.9% purity acetonitrile (Fisher Scientific; Cat.A998-4) ≥ 99.8% purity methanol (Fisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... The following primary antibodies and dilutions were used overnight at 4 °C in PBST with 1% milk: mouse anti- V5 conjugated to horseradish peroxidase (HRP; 1:5,000; Invitrogen), mouse anti-V5 (1:5,000 ...
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bioRxiv - Cell Biology 2023Quote: ... This was followed by overnight incubation at 4°C with 1:200 anti-LAMP-1 (D401sm #15665, Life Technologies). The next day ...
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bioRxiv - Cell Biology 2021Quote: ... Secondary antibodies were HRP-conjugated goat anti-rabbit IgG (G-21234; 1:25000; Life Technologies) and goat anti-mouse IgG (G-21040 ...
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bioRxiv - Microbiology 2019Quote: ... or 1:5,000 HRP-conjugated goat anti-mouse IgG antibody (Thermo Fisher Scientific, G-21040) were used as secondary antibodies ...
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bioRxiv - Molecular Biology 2022Quote: ... the supernatant was incubated with 1/14 volume of free Protein G Dynabeads (ThermoFisher Scientific) rolling for 1 hour at 5°C to remove sticky proteins and RNAs ...
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bioRxiv - Biochemistry 2022Quote: ... followed by secondary AlexaFluor 647 goat anti-mouse immunoglobulin G (1:1,000, Invitrogen A-21235) with Hoechst 33342 dye (1:3,000 ...
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bioRxiv - Microbiology 2020Quote: ... 1 µg of purified antibodies was conjugated to 10 µl Dynabeads Protein G (Thermo Fisher) for 30 min at room temperature ...
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bioRxiv - Biochemistry 2019Quote: ... the supernatant was incubated with 25 μL (1:10 dilution) protein G beads (Invitrogen, 10003D) and 1-2 μg of antibodies for 4 h to overnight ...
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bioRxiv - Cancer Biology 2019Quote: ... Mice were then injected with 1 mg/g D-glucose (Thermo Fisher Scientific A24940-01) and their blood glucose monitored over 180 minutes ...
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bioRxiv - Biochemistry 2020Quote: ... Cells were in standard culture conditions (87.5% D-MEM (containing 1 g glucose/L) (Gibco), 1% Non-Essential Amino Acids (Gibco) ...
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bioRxiv - Genetics 2021Quote: ... embryos were stained with 1 μg/ml DAPI and mounted with Fluoromount-G (ThermoFisher Scientific). Images were taken with ZEISS ZEN Software on a Zeiss LSM710 confocal laser scanning microscope (Carl Zeiss ...
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bioRxiv - Synthetic Biology 2022Quote: ... The cells were then starved in DMEM low glucose 1 g/l (Gibco, 11885-084) serum-free media per plate for 16 hours ...
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bioRxiv - Systems Biology 2022Quote: HEK293T and LX293T cells were cultured in 1 g/L glucose DMEM (Life Technologies 11885076), 1% Antibiotic-Antimycotic (Thermo 15240062) ...
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bioRxiv - Neuroscience 2022Quote: A subcutaneous injection of sulforhodamine 101 (SR 101, Thermo Fisher Scientific, 0.2 μl g-1) was administered in mice to visualize the cerebral vasculature in vivo ...
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bioRxiv - Molecular Biology 2022Quote: ... AEBSF 1 mM) with 2 µg of antibodies and Dynabeads Protein G (Thermo Fisher Scientific). Control immunoprecipitation (IP ...
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bioRxiv - Cell Biology 2022Quote: ... 1 mM EDTA) and loaded onto magnetic beads (dynabeads, protein G, 2.8 μm diameter, Invitrogen), which were previously decorated with sub-saturating quantities of a monoclonal anti-FLAG antibody (M2 ...
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bioRxiv - Molecular Biology 2023Quote: ... 1 mg lysate was pre-cleared with Dynabeads™ protein G (cat# 10004D, Thermo Fisher) for 1 h with rotation at 4°C ...
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bioRxiv - Biochemistry 2023Quote: ... then 1 hour with Pierce™ Protein A/G agarose beads (Thermo Fisher Scientific, 20421). Beads were washed 4 times with 0.1% NP-40 substitute (VWR ...
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bioRxiv - Microbiology 2023Quote: ... powdered skim milk 50 g/L was added to autoclaved 1% non-nutrient agar (Invitrogen) as described (Park et al. ...
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bioRxiv - Molecular Biology 2023Quote: ... Dry cells were mounted with 1 drop of Fluoromount-G™ (#15586276, Invitrogen, Fisher Scientific), covered with cover slide (#4818602 ...
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bioRxiv - Molecular Biology 2023Quote: ... Dry cells were mounted with 1 drop of Fluoromount-G™ (#15586276, Invitrogen, Fisher Scientific), covered with cover slide (#4818602 ...
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bioRxiv - Biophysics 2023Quote: ... 02238131) and 1% penicillin-streptomycin solution (10.000 Units/ml Penicillin, 10.000 g/ml Streptomycin, Gibco). Jurkat cells (gift from Prof ...
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bioRxiv - Bioengineering 2020Quote: ... the ADSC and the HUVEC were added after trypsin detachment (both always used at passages 1-6) and centrifugation 1 minute at 3,500 rpm (Minispin, ThermoFisher Scientific, Whaltam, MA, USA) to get a final cell concentration of 4×106 ADSC/mL and 2×106 HUVEC/mL ...
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bioRxiv - Plant Biology 2021Quote: ... The liquid syringe was washed 6 times with each of 10 µL of 1:1 (v/v) acetone:hexane and HPLC grade methanol (Fisher Scientific Co, Edmonton Alberta) both before and after the liquid injection.
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bioRxiv - Developmental Biology 2022Quote: Total RNA was isolated from 6 pairs of P21 mouse testes (3 pairs of wild type and 3 pairs of Mov10-/-) using TRIzol reagents (Thermo Fisher Scientific). 1 μg of total RNA from each sample was used to generate RNA-seq libraries using TruSeq Stranded mRNA Library Preparation Kit Set A (Cat ...
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bioRxiv - Pathology 2024Quote: Tear samples were thawed at 4°C on ice and the total protein concentration was measured with a NanoDrop One Microvolume UV-Vis Spectrophotometer (Thermo Fisher Scientific, reference ND- ONE-W) by using 2µL of sample ...
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bioRxiv - Immunology 2020Quote: ... 25 μl/well of 60 mM water solution of 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC, Thermo Fisher Scientific) were added ...
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bioRxiv - Neuroscience 2023Quote: ... The carboxylic groups on the carbon fiber surface were electro-activated by incubation in 0.4 M 1-ethyl-3-[3-dimethylaminopropyl] carbodiimide hydrochloride (EDC; Life Technologies) and 0.1 M and N-hydroxysulfosuccinimide (NHSS ...
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bioRxiv - Synthetic Biology 2023Quote: ... 5 g/L ammonium sulphate (Fisher Chemical™) and 1.7 g/L yeast nitrogen base without amino acids (Thermo Scientific™) and was supplemented with 2% (w/v ...
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bioRxiv - Bioengineering 2022Quote: ... 5×5 minute PBST washes were performed followed by staining with Hoechst (1:2000; Thermo Fisher H3570) for 10 minutes at room temperature ...
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bioRxiv - Evolutionary Biology 2023Quote: ... and mixed with 12 μl of 5-CF AM solution (Thermo Fisher Scienfic, 5 mg ml-1). The cells were incubated in dim light at 30 °C for 30 min before harvesting and washing 3 times in fresh medium ...
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bioRxiv - Bioengineering 2021Quote: ... Apoptosis was detected by incubating devices with 5μM of CellEvent™Caspase 3/7 Green detection reagent (Invitrogen) for 30 minutes at 37°C.
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bioRxiv - Cancer Biology 2021Quote: ... After 3 and 7 days of treatment cells were stained with 2% crystal violet (CV) (40583100, Acros Organics, Fisher Scientific ...
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bioRxiv - Immunology 2020Quote: ... splenic NP366-374 TMEM were assessed with CellEvent™ Caspase-3/7 Green Flow Cytometry Assay kit (ThermoFisher). Lung single cells were stained with surface markers then incubated with caspase 3/7 green detection reagent for 30 minutes at 37°C as described in the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... the cells were loaded with 2 µM CellEvent™ Caspase-3/7 Green Detection Reagent (Thermo Fisher Scientific), according to the manufacturer’s instructions for kinetic assays and fluorescence in the live cells ...
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bioRxiv - Cell Biology 2022Quote: Live/Dead™ Cell Imaging Kit and CellEvent™ Caspase-3/7 Green Detection Reagent (Thermo Fisher Scientific) were used for detection of cell apoptosis based on the manufacturer instructions ...
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bioRxiv - Immunology 2023Quote: ... 10% H2/N2 (BOC) for 3 days on blood agar plates containing 7% laked horse blood (Thermo Scientific) and the Campylobacter selective supplement “Skirrow” containing the antibiotics trimethoprim ...
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bioRxiv - Microbiology 2022Quote: ... Proliferating organoids were grown for 4–7 days and dissociated using TrypLE Express Enzyme (Thermo Fisher Scientific) for 10–20 minutes in a 37°C water bath ...
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bioRxiv - Developmental Biology 2021Quote: ... Cell between passage 4 to 7 were transfected with si-SLC38A5 siRNA (Cat# 4392420, Thermo Fisher Scientific) or negative control siRNA (si-Ctrl ...
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bioRxiv - Microbiology 2023Quote: ... Parasites (CDC1132 or G3 strain) were labelled with Cell Tracker Blue CMAC (7-amino-4-chloromethylcoumarin) (Invitrogen), added to confluent BPH-1 cells (1:3 parasite ...
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bioRxiv - Neuroscience 2019Quote: ... Solubilized material was centrifuged at 20,000 g for 10 minutes at 4°C and the supernatant was precleared with protein A/G sepharose beads (Thermo Fisher Scientific) for 30 minutes ...
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bioRxiv - Genomics 2023Quote: ... The EDNRB2 gene SNP mutation (g.4:12583552 G>A) was genotyped by Sanger sequencing on an ABI 3730xl DNA Analyzer (Applied Biosystems, USA) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... Extracted fungal masses were plunged into a solid medium (7.8 g/L potato dextrose agar [BD], 6 g/L agar [Difco], 100 mg/L Penicillin/Streptomycin [Gibco], and 100 mg/L Kanamycin [Alfa Asear]) and maintained at 28°C for 15 days to screen for possible contaminants and indications of sample viability ...
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bioRxiv - Cell Biology 2020Quote: ... followed by a 1.5 hrs protein-chromatin pull-down with a 1:1 mix of protein A and protein G Dynabeads (Thermo Fisher; 10001D and 10003D). ChIP samples were washed extensively ...
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bioRxiv - Cancer Biology 2020Quote: ... to 4 parts Dulbeco Modified Eagle Medium (DMEM) low glucose (1 g/mL) + sodium pyruvate 110 mg/mL + 1% Penicillin-Streptomycin (10,000 U/mL; Thermo Fisher Scientific, Waltham, MA), supplemented with 5% fetal bovine serum (Atlanta Biologicals ...