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Citations for Thermo Fisher :
3051 - 3100 of 10000+ citations for FabFc ZAP rat Antibody Internalization Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... Primary antibodies were detected using Alexa Fluor-labelled secondary antibodies (Life Technologies) while DAPI (Sigma ...
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bioRxiv - Biophysics 2024Quote: ... The following secondary antibodies were used: goat anti-mouse antibody (31160, Invitrogen) and goat anti-rabbit antibody (31210 ...
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bioRxiv - Cell Biology 2020Quote: ... 1:500 (all from the Developmental Studies Hybridoma Bank and anti-mouse and anti-rat IgG Alexa Fluors 568 and 647, from Molecular Probes); some secondary antibodies were preabsorbed with fixed y w embryos ...
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bioRxiv - Developmental Biology 2021Quote: ... anti-rabbit IgG Alexa 647 (A21244) goat anti-rat IgG Alexa 488 (A11006) and Alexa 488 conjugated phalloidin (A12379) were purchased from Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... the sections were incubated with 4% paraformaldehyde in 0.1 M phosphate buffer for 30 min at 37 °C and then incubated with rat anti-PROM1 (Thermo Fisher Scientific) overnight at 4 °C ...
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bioRxiv - Microbiology 2020Quote: ... 2×104 cells were resuspended in serum-free medium and applied to the top of a membrane coated with collagen I (rat-tail) (Gibco- A1048301). Transmigration assays were carried out using a Corning Transwell Costar apparatus (6.5 mm diameter and 8 µm pore size ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were washed with PBS and incubated with secondary anti-rabbit and/or anti-mouse or anti-rat IgG conjugated with Alexa-Flour 488 or Alexa-Flour 568 (Molecular Probes) for 1 h at room temperature ...
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bioRxiv - Developmental Biology 2021Quote: ... 2×103 sorted naked mole-rat cells were added to 3ml of HSC005 supplemented with 1% Penicillin/Streptomycin and 1X GlutaMAX (both Thermo Fisher), grown for 14d at 32°C ...
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bioRxiv - Cell Biology 2021Quote: ... followed by washes in PBS + 0.05% Tween-20 and 30 minutes incubation in goat anti-mouse Alexa fluor 633 and goat anti-rat Alexa fluor 488 (1:25, A21050 and A11006, Molecular Probes). CombiCoverslips were mounted with ProLong Diamond Antifade reagent (P36965 ...
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bioRxiv - Molecular Biology 2021Quote: ... media with 100 nM siRNA (rat siGabarapl1; siGENOME SMARTpool, Dharmacon; Figure S4a) vs non-targeting scrambled siRNA with Lipofectamine RNAiMAX (Life Technologies) for 16 hours ...
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bioRxiv - Microbiology 2021Quote: ... wells of 96 wells plate were coated with 100 µL of 40 µg.mL-1 rat tail type I collagen in sterile PBS (Life Technologies, NY) at 4°C for 18 hours ...
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bioRxiv - Molecular Biology 2021Quote: Deep-sequencing of total RNA isolated from neonatal rat cardiomyocyte cultures was performed 72 hr after transfection of mimics (Life Technologies) of hsa-miR-106b-5p ...
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Naked Mole-Rat Hematopoietic Stem and Progenitors are Highly Quiescent with an Inherent Myeloid BiasbioRxiv - Cell Biology 2021Quote: ... Either 1x104 whole marrow or 1x103 sorted naked mole-rat cells were added to 3ml of HSC005 supplemented with 1% Penicillin/Streptomycin and 1X GlutaMAX (both Thermo Fisher), equally divided into two 35mm dishes ...
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bioRxiv - Cell Biology 2021Quote: ... Collagen matrix was prepared on ice by adding 10x PBS (to make 1x PBS) and 1N NaOH (to make 25mM NaOH) to Collagen I (Rat tail, Thermo Fisher). Organoids were treated with ERW medium ...
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bioRxiv - Microbiology 2022Quote: ... Cells were then incubated for 30 min with goat anti-rabbit Cy5 (Immunoresearch 111 175 144) or goat anti-rat FITC (Invitrogen A11006) secondary antibodies and DAPI (10 μg/mL) ...
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bioRxiv - Neuroscience 2022Quote: ... sections were washed four times for 5 min with PBS and incubated for 2 h in the dark with Alexa Fluor 568-conjugated donkey anti-rat IgG (Life Technologies) at a dilution of 1:300 ...
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bioRxiv - Cell Biology 2022Quote: ... the cerebral cortices of 4 to 6 P0 rats were dissociated and chopped in Ca++/Mg++-free Hank’s Balanced Salt Solution (HBSS; Invitrogen, Carlsbad, CA). The tissue pieces were dissociated in phosphate-buffered saline (PBS ...
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bioRxiv - Neuroscience 2022Quote: ... The secondary antibodies were Alexa Fluor 488 goat anti-mouse (green) and Alexa Fluor 568 goat anti-rat (red) (used at 1:250 dilution in blocking solution, Life Technologies).
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bioRxiv - Neuroscience 2022Quote: We extracted total RNA from PFC and NAc tissues dissected from 6 rat brains using Trizol Reagent (Invitrogen, Cat, num. 15596018) and Zirconium Beads RNase Free (Next Advance ...
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bioRxiv - Bioengineering 2022Quote: hCMEC/D3 brain endothelial cell line was maintained in tissue culture flasks pre-coated with 0.15 mg/mL rat collagen I in a humidified incubator at 37 ºC and 5% CO2 (Isotemp, Thermo Fisher Scientific). hCMEC/D3 cells were maintained in complete growth medium comprising of endothelial cell basal medium (EBM-2 ...
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bioRxiv - Neuroscience 2021Quote: ... 20μm sections were incubated with fluorescently conjugated antibodies directed either against mouse IgG, or the IgG of control species (rat, goat or rabbit) (all raised in donkey, Thermo Fisher) in PBS for 2h ...
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bioRxiv - Neuroscience 2021Quote: ... RTN3 was knocked down in DRGN/retinal cultures using an siRNA to rat RTN3 (siRTN3; Assay ID 251374, cat no. AM16708, ThermoFisher Scientific). Protrudin (siProtrudin ...
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bioRxiv - Cell Biology 2021Quote: ... were isolated from 3-4 days old euthanized rats and were plated in α-MEM medium supplemented with 10% fetal bovine serum (Gibco) and 1% Penicillin-Streptomycin solution (Hyclone ...
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bioRxiv - Neuroscience 2020Quote: ... The sections were then incubated with the following secondary antibodies for 3 h at room temperature: anti-rabbit IgG-Alexa488 and anti-rat IgG-Alexa488 (1:1000 for both; Invitrogen Co). Sections were mounted on microscope slides and embedded with Vectashield that includes DAPI for nuclear staining (Vector Laboratories ...
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bioRxiv - Microbiology 2022Quote: ... PostF APC/DyLight755 and PE/Dylight650 conjugated tetramers were added at a final concentration of 25 nM in the presence of 2% rat and mouse serum (Thermo Fisher) and incubated at room temperature for 10 min ...
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bioRxiv - Cell Biology 2019Quote: ... Endothelial cells were then purified by a 10-minute incubation in suspension with magnetic sheep anti-rat Dynabeads (Invitrogen, Carlsbad, CA) coated with rat anti-mouse PECAM-1 (rat ...
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bioRxiv - Cancer Biology 2020Quote: ... 2×104 cells were seeded on top of 1.7mg/mL collagen (type I, rat tail) with or without Matrigel matrix (both from Corning, via Thermo Fisher Scientific) supplemented with 10% minimum essential medium alpha medium (MEMalpha ...
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bioRxiv - Neuroscience 2021Quote: ... Appropriate secondary fluorescent antibodies were used (Alexa Fluor 488 Goat Anti-rat, #32731; 1:1000; Invitrogen, US; and Alexa Fluor 568 Goat Anti-rabbit, #11011; 1:1000; Invitrogen, US). For Cell nuclei labeling ...
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bioRxiv - Developmental Biology 2022Quote: ... tissues were incubated with 1:200 anti-rat IgG 647 for 2 h at room temperature with 1:500 phalloidin TRITC (Fisher Scientific). Then ...
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bioRxiv - Immunology 2020Quote: ... PostF APC/DyLight755 conjugated tetramers were added at a final concentration of 25 nM in the presence of 2% rat and mouse serum (Thermo Fisher) and incubated at room temperature for 10 min ...
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bioRxiv - Molecular Biology 2021Quote: ... and HEK293T cells derived from human embryonic kidney and transformed with the SV40 large T-antigen were cultured in Dulbecco’s Modified Eagle Medium (Nissui). ARL cells (rat liver epithelial cells; Japan Health Sci. Found., JCRB0248) were cultured in Williams’ Medium E (Gibco BRL). Media were supplemented with 5% fetal calf serum (Gibco BRL) ...
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bioRxiv - Immunology 2021Quote: ... Bacteria were then washed and bound IgA was detected with a PE-conjugated rat anti-mouse IgA (mA-6E1, 4 μg/ml; Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2021Quote: ... HiPerFect transfection reagent was used to transfect cells with small interfering (si) RNA oligoribonucleotides targeted to rat ATF6 (assay ID# RSS315363, Stealth siRNA, Thermo Fisher). A non-targeting sequence (cat# 12935300 ...
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bioRxiv - Neuroscience 2020Quote: ... of 0.1M PBS (pH 7.4) followed by 180 ml (rats) or 20 ml (mice) of fresh 4% paraformaldehyde (PFA, pH 7.4) (Fisher Scientific, Hampton NH). Brains were rapidly removed ...
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bioRxiv - Genetics 2019Quote: ... 15 minutes each at 4°C) in PBS-T 0.05% and incubated with either an Alexa Fluor 488 goat anti-rat IgG (Thermo Fisher Scientific) or an Alexa Fluor 488 goat anti-rabbit IgG (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2022Quote: ... The FACS sorted cells were then seeded at a density of 2e4 cells per well in rat tail collagen (Invitrogen, A1048301) coated 96-well CellCarrier plates (PerkinElmer ...
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bioRxiv - Genetics 2022Quote: ... primary rat astrocytes were thawed into T75 flasks and fed once every 2-3 days with glial media {(MEM (Thermofisher 11095080) supplemented with 5% FBS (Gemini 900-108) ...
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bioRxiv - Physiology 2022Quote: ... EP3R immunoreactivity was visualized by reaction with FITC-conjugated tyramide as described above (for rats injected with Alexa647-conjugated CTb) or with Alexa647-conjugated tyramide (1:50–1:100; B40958, Thermo Fisher) in PBS containing 0.0002– 0.0015% of H2O2 for 5 min (for rats injected with Alexa488-conjugated CTb) ...
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bioRxiv - Molecular Biology 2022Quote: Total RNA of was isolated from cardiac fibroblasts as described above and subjected to GeneChip analysis (GeneChip Arrays platform with Clariom™ S Assay, Rat; Affymetrix). Two independent biological replicates were prepared for cardiac fibroblasts ...
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bioRxiv - Neuroscience 2022Quote: ... Rat pheochromocytoma cell line PC12 (ATCC Cat# CRL-1721; RRID:CVCL_0481, male) was grown in RPMI 1640 media (Gibco Cat#11875-101) containing 10% horse serum (HS ...
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bioRxiv - Neuroscience 2023Quote: ... Spinal cords isolated from embryonic day 14 Sprague Dawley rat were dissociated enzymatically in trypsin supplemented with 0.25% EDTA (Thermo Fisher Scientific) and physically by trituration ...
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bioRxiv - Neuroscience 2022Quote: ... 25 ng of plasmids containing sgRNAs for respective genes were transfected into rat primary neurons on DIV 4 using Lipofectamine 2000 (ThermoFisher, #11668027) along with 50 ng of TDP43(WT)-EGFP or mApple then survival was measured over the course of 10 days ...
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bioRxiv - Cell Biology 2022Quote: ... HRP-conjugated goat anti-rat IgG (catalog #62-9520) and Zysorbin-G (catalog #10-1051-1) were from Invitrogen (Carlsbad, CA). The mouse IgG1 (catalog #401407) ...
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bioRxiv - Plant Biology 2024Quote: ... Immunolabelling experiments were performed with LM19 and LM20 (1:10 dilution in TTBS-milk, 2 h incubation) followed by anti-rat IgG-Alexa488 (Invitrogen®) or anti-rat IgG alkaline phosphatase conjugate (Sigma-Aldrich A8438 ...
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bioRxiv - Cell Biology 2024Quote: The hippocampus was dissected from TP18-day Sprague Dawley rat fetuses of either sex using cold dissection medium L-15 (Gibco, 21083027). Subsequently ...
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bioRxiv - Cell Biology 2023Quote: ... The surface of the gel was then rendered permissive to cell adhesion by coating Rat Tail collagen I (Gibco, Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... Barcoded samples were then combined and blocked for 15 min at 4°C with 100 μl of mouse and rat serum (both from Thermo Fisher) diluted at a 1:33 (v/v ...
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bioRxiv - Microbiology 2024Quote: ... Tissue sections were incubated with rat anti-mouse primary monoclonal mast cell protease-1 (MCPT-1) Ab (1:100, clone RF6.1; Thermo Fisher Scientific), followed by secondary staining with biotinylated rabbit anti-rat IgG (Abcam) ...
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bioRxiv - Immunology 2023Quote: ... the appropriate secondary antibodies were added diluted in 1% BSA in PBS for 1 hour at a 1:1000 dilution (Goat anti Rat 488 Life Technologies a11006 ...
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bioRxiv - Neuroscience 2023Quote: ... sections were washed 3 times with PBS and incubated in secondary Alexa Fluor 555 conjugated goat anti-rat (Invitrogen, A-21434) and secondary Alexa Fluor 488 conjugated goat anti-rabbit (Invitrogen ...