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Citations for Thermo Fisher :
3301 - 3350 of 10000+ citations for FabFc ZAP rat Antibody Internalization Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... cells were seeded onto a 96-well microtiter IncuCyte® ImageLock Plate (Essen BioScience) pre-coated with rat tail Collagen type I (Gibco™). When cells were at full confluence ...
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bioRxiv - Immunology 2021Quote: ... anti-CD326/EpCAM [G8.8] (PE-Cy7) anti-CD107a [eBio1D4B] (PE) and Rat IgG2a kappa Isotype control [eBR2a] (PE) were purchased from Thermo Fisher (Waltham, Massachusetts). Anti-KLRG1 [MAFA] (APC) ...
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Development of follicular dendritic cells in lymph nodes depends on retinoic acid mediated signalingbioRxiv - Immunology 2020Quote: ... and unlabeled anti-LTβR mAb and subsequently counterstained with streptavidin-Alexa 488 and goat-anti-rat-Alexa 488 (all Invitrogen, Breda, The Netherlands), respectively ...
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bioRxiv - Bioengineering 2022Quote: ... The rat tail collagen I protein and GFOGER peptide peptide were subsequently blocked using methyl-PEG24-amine (MA-PEG24) (Thermo Fisher Scientific). Post functionalization ...
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bioRxiv - Cell Biology 2022Quote: ... Alexa Fluor 488 donkey anti-mouse IgG and Alexa Fluor 647 donkey-rat IgG (1:1,000; Thermo Fisher Scientific, Rockford, IL, USA). Liver ...
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bioRxiv - Developmental Biology 2022Quote: ... goat anti-rabbit IgG H&L (Alexa Fluor®) 568 & goat anti-rat IgG H&L (Alexa Fluor®) 568 was from ThermoFisher and was used at a 1:400-fold dilution ...
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bioRxiv - Neuroscience 2022Quote: ... rats of either sex and then digested in papain (0.5 mg/mL in Earle’s balanced salt solution) (Thermo Fisher Scientific Inc., MA). Dissociated cells were washed with and triturated in 10% heat-inactivated fetal bovine serum (FBS ...
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bioRxiv - Neuroscience 2023Quote: ... Secondary antibodies (Donkey anti-goat alexa fluor 594, Donkey anti-rabbit alexa fluor 594, Donkey antirabbit alexa fluor 488, Donkey anti-rat alexa fluor 594; Invitrogen, 1:1000) were incubated with PBT-BSA for 1h at room temperature and 24h at 4°C ...
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bioRxiv - Molecular Biology 2024Quote: ... transfected cells were seeded at the density of 2000 cells/well in 96-well microtiter IncuCyte® ImageLock Plate (Essen BioScience) pre-coated with rat tail Collagen type I (Gibco BRL) overnight ...
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bioRxiv - Neuroscience 2024Quote: ... Alexa Fluor goat anti-rabbit 594, A11037; Alexa Fluor goat anti-rabbit 488, A11008; Alexa Fluor goat anti-rat 594, A11007; Invitrogen, Waltham, MA) before mounting with DAPI Fluoromount-G (0100-20 ...
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bioRxiv - Immunology 2024Quote: ... Slides were washed in PBS-Tween (0.5% Tween 20 in 1x PBS) and incubated in goat anti-rat IgG Alexa Fluor® 488 (1:200; A11006; Life Technologies) antibody for 45 min at room temperature ...
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bioRxiv - Molecular Biology 2023Quote: ... Alexa Fluor 568 goat anti-mouse IgG (H+L) and Alexa Fluor 488 goat anti-rat IgG (H+L) (both from Invitrogen Molecular Probes), for 1 h ...
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bioRxiv - Cell Biology 2023Quote: Rat Basophilic Leukemia (RBL-2H3) cells (ATCC, CRL-2256) were maintained in monolayer cultures with MEM growth medium (Life Technologies, Carlsbad, CA) supplemented 20% heat inactivated Fetal Bovine Serum (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2023Quote: ... NRVMs were isolated and cultured as previously described.23 The hearts from D0-3 rat pups were isolated and rinsed in HBSS (ThermoFisher Scientific, 14025092) before placed in trypsin-EDTA 0.05% (ThermoFisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... Astrocytes and microglia/macrophage cells were labeled using rat anti-GFAP (1:300; Cat# 13-0300, Thermo Fisher Scientific, Waltham, MA, U.S.A) and rabbit anti-IBA1 (1:500 ...
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bioRxiv - Biochemistry 2023Quote: ... Detection of bound Af2-IL was performed using a rat anti-human IL-2 mAb Alexa Fluor488 conjugate (Invitrogen, Carlsbad CA, USA). Cell nuclei were counterstained with DAPI (Sigma Aldrich ...
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Plasma Activated Water as a Pre-Treatment Strategy in the Context of Biofilm-Infected Chronic WoundsbioRxiv - Microbiology 2023Quote: ... wells of 96-well flat bottom microtiter plates were pre-coated with 300 µg/mL collagen I from rat tail (Gibco, Life Technologies, USA) for 1 h at 37°C ...
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bioRxiv - Developmental Biology 2023Quote: ... 1% w/v Triton X-100 and incubated with goat anti-rat Alexa Fluor 488 (1:1000, catalog no. A11029, Thermo Fisher Scientific) secondary antibody ...
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bioRxiv - Genetics 2023Quote: ... antibodies for 1.5 h and then with corresponding secondary goat anti-rat Alexa594 and anti-mouse Alexa488 (A-11007 and A-11001, Thermo Fisher Scientific) antibodies (1:300 ...
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A spatially resolved EGFR signaling model predicts the length scale of GAB1-SHP2 complex persistencebioRxiv - Systems Biology 2023Quote: ... EGFR was detected by incubating coverslips for 1 hr at 37°C with anti-rat Alexa Fluor 594 (A-11007, 1:750; Invitrogen, Waltham, MA) and Hoechst nuclear stain (1:2,000) ...
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bioRxiv - Bioengineering 2023Quote: ... at 4°C overnight and then alkaline phosphatase (AP) conjugated anti-mouse IgG adsorbed with rat serum protein) (Thermo Fisher Scientific, USA) for 2 h diluted 1 ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were then washed twice into RPMI medium and seeded in a rat tail collagen-1 (5μg/cm2; Gibco, Carlsbad, CA, USA) coated flask for cell culture ...
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bioRxiv - Cell Biology 2023Quote: ... 5’-GAGACCCUAUCCGUGAUUAtt-3’ and antisense: 5’- UAAUCACGGAUAGGGUCUCtt-3’ (Silencer Select, rat negative control #1; scrambled siRNA and all siRNAs were from Ambion, Life Technologies). Transfection complexes were prepared in accordance with the instructions provided by the manufacturer and added to 2×105 cells seeded per well in 24-well plates.
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bioRxiv - Immunology 2023Quote: ... Intracellular cytokines were stained at 4°C overnight with FITC or PerCP-e710-conjugated rat anti-mouse IL-1β (NJTEN3; Thermo Fisher Scientific) and PE-conjugated Armenian hamster anti-mouse IL-1α (ALF-161 ...
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bioRxiv - Immunology 2024Quote: ... Whole- mounts were then washed 3 times for 5 min at room temperature in PBS followed by incubation with Alexa Fluor 568 anti-rat (Invitrogen, 1:500) for 1 hour at room temperature in PBS with 1% BSA and 0.5% Triton-X-100 ...
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bioRxiv - Immunology 2024Quote: ... rat or rabbit IgG (Invitrogen Clone A10043 Lot 753765; Invitrogen Clone A11055 Lot 2211210; Invitrogen A21447 Lot 872645; Invitrogen A10043 Lot 753765) at 1:500 in PBS+0.3% triton X-100 overnight ...
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bioRxiv - Cell Biology 2024Quote: ... and rat pAbs horseradish peroxidase-conjugated (HRP-Ab) (#111-035-003, #115-035-003, Jackson Immunoresearch Laboratories; #31470, Invitrogen; 1:5000 dilution).
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bioRxiv - Molecular Biology 2024Quote: ... with 100 nM of either ON-TARGETplus Non-targeting Control siRNA Pool (Dharmacon #D-001810-10-05) or On-TARGETplus Smartpool siRNA targeting rat ACLY protein (Dharmacon #L-089773-02-0005) using lipofectamine RNAimax (Thermofisher Scientific #13778075), as per manufacturer’s protocol.
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bioRxiv - Bioengineering 2019Quote: ... they were further incubated with a secondary HRP-conjugated goat anti-rabbit IgG antibody for 2 hours at room temperature and imaged using a supersignal west pico enhanced chemiluminescence kit (Thermo Fisher Scientific, Suwanee, GA,USA). The signals were visualized with UVP software.
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bioRxiv - Cancer Biology 2019Quote: ... FYN antibody was conjugated with Alexa Fluor™ 488 Tyramide SuperBoost™ Kit - Goat Anti-Rabbit IgG (# B40922) following the manufacture instructions (Invitrogen-Thermo Fisher Scientific).
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bioRxiv - Immunology 2021Quote: ... was performed using IAV-NP mAb (IAV-NP HB-65) as primary antibody and Alexa fluor 594 goat anti-mouse antibody as secondary antibody (Invitrogen, catalog no: A11005). SIV infected cells were identified based on the presence of fluorescent foci.
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bioRxiv - Cancer Biology 2020Quote: ... peroxidase-linked species-specific whole antibody (from sheep) secondary antibody (45-000-679, 1:5000-50000 depending on the primary antibody) were both from Fisher Scientific (Waltham, MA). Immunoblots were imaged using Immobilon Western Chemiluminescent HRP Substrate (ECL ...
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bioRxiv - Microbiology 2022Quote: ... the cells were stained overnight at 4 °C with E1 or nsP1 primary rabbit antibodies (both antibodies previously validated in our laboratory) [11] followed by Alexa Fluor 568 goat anti-rabbit secondary antibody (Invitrogen, Carlsbad, CA, USA). Finally ...
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Maize AFP1 confers antifungal activity by inhibiting chitin deacetylases from a broad range of fungibioRxiv - Microbiology 2021Quote: ... One OD600 of cells washed with MES buffer was subjected to immunostaining using the primary anti-HA antibody and AF488-conjugated secondary antibody or wheat germ agglutinin conjugated antibody (WGA-A488; Molecular Probes, Karlsruhe, Germany) to localize CDA proteins or chitin respectively ...
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bioRxiv - Neuroscience 2024Quote: ... or HRP-conjugated secondary antibodies (Chicken anti Mouse secondary antibody HRP, Fisher Scientific # A15981, Sheep anti Rabbit secondary antibody HRP, Fisher Scientific #A16172) at RT for 1 h ...
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bioRxiv - Molecular Biology 2023Quote: ... followed by incubation with corresponding alkaline phosphatase-coupled secondary antibodies (goat anti-rabbit polyclonal antibody, Thermo Scientific, 1:10000; or goat anti-mouse polyclonal antibody, Invitrogen, 1:10000). The presence of the antigens of interest was observed with Enhanced Chemifluorescence substrate (ECF ...
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bioRxiv - Microbiology 2023Quote: ... cells were washed thoroughly with cold PBS to remove unbound primary antibody and a secondary antibody (Alexa 488 goat anti-mouse IgG secondary antibody (Invitrogen, OR Waltham, MA)) at a 1:750 dilution in PBS was applied to the samples for 1 hour at room temperature ...
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bioRxiv - Cancer Biology 2021Quote: ... we used the following primary antibodies: mouse anti-V5 monoclonal antibody (46-0705, Invitrogen), rabbit anti-FOXA1 M2 polyclonal antibody (GTX100308 ...
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bioRxiv - Biophysics 2020Quote: ... Primary antibodies were detected with Alexafluor-594 and Alexafluor-488-conjugated secondary antibodies (Invitrogen). Nuclei were stained with DAPI and cells were imaged using the Zeiss LSM700 system.
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bioRxiv - Cancer Biology 2021Quote: ... The membranes were incubated with primary antibodies (PTPRH antibody, 1:1000, Fisher Scientific, #PIPA531340) overnight at 4°C ...
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bioRxiv - Genetics 2021Quote: ... Antibody detection was performed using AlexaFluor (488 and 568) conjugated secondary antibodies from Invitrogen at 1:800 dilution in blocking solution overnight at 4°C followed by washes with 1% Tween-20 in PBS.
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bioRxiv - Cancer Biology 2021Quote: ... The GFP antibody was paired with Alexa 647-conjugated anti-chicken secondary antibody (Invitrogen) to avoid confounding tissue autofluorescence ...
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bioRxiv - Developmental Biology 2020Quote: ... Secondary antibodies were Alexa Fluor-488 and −555 conjugated antibodies (1:1000, Molecular Probes) and goat anti-Chicken Alexa Fluor-488 (1/800 ...
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bioRxiv - Molecular Biology 2021Quote: Antibodies for immunoblots: primary antibodies included anti-myc monoclonal 9E10 (Thermofisher, diluted 1:10,000) or anti-G6PDH (Sigma ...
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bioRxiv - Microbiology 2019Quote: ... and anti-SAG1 (DG52) primary antibodies and polyclonal Alexa Fluor-conjugated secondary antibodies (Invitrogen). Partial permeabilization of a particular vacuole was determined by the exclusion of the SAG1 antibody.
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bioRxiv - Biophysics 2019Quote: ... (3) antibody solution consisting of 100 μg/ml biotin tubulin antibodies (Thermo Fisher Scientific) in PBS that bind specifically to the F(ab’)2 fragments (incubation time 5 min) ...
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bioRxiv - Neuroscience 2020Quote: ... and secondary antibody: Goat anti-mouse Alexa-488 secondary antibody (Invitrogen, A11034, 1:500) and goat anti-mouse Alexa-647 secondary antibody (Invitrogen ...
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bioRxiv - Developmental Biology 2020Quote: ... Secondary antibodies used: AlexaFluor 405-labeled goat anti-mouse antibody (1:500; ThermoFisher #A31553). Actin was stained by incubation (30-120min at RT ...
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bioRxiv - Cell Biology 2021Quote: ... Secondary Alexa Fluor antibodies and HRP conjugated secondary antibodies were procured from Life technologies.
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bioRxiv - Cancer Biology 2022Quote: ... Secondary antibodies of anti-mouse and anti-rabbit antibodies were ordered from Thermo Fisher Scientific ...