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Citations for Thermo Fisher :
2951 - 3000 of 10000+ citations for FabFc ZAP rat Antibody Internalization Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... Following application of Alexa 596 anti-rabbit IgG and Alexa 488 anti-rat IgG (Invitrogen, 1:1000 for 1 hour), cells were fixed in 50:50 acetic acid and ethanol for 10 minutes and DNA was denatured in 2M HCl for 30 minutes ...
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bioRxiv - Developmental Biology 2022Quote: The ten raw files were searched against the Swiss-Prot rat database with SEQUEST HT by Proteome Discoverer (version 2.1; Thermo Scientific). A maximum of two missed cleavage sites were allowed in the trypsin digestion ...
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bioRxiv - Cell Biology 2022Quote: ... embryos were incubated for two hours at room temperature with fluorescently-labelled secondary antibodies diluted 1:100 in BBT (Alexa 488-conjugated goat anti-rabbit IgG, Alexa 555-conjugated goat anti-rat IgG, Alexa 647-conjugated goat anti-mouse IgG; Invitrogen). After five final washes in PBT ...
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bioRxiv - Developmental Biology 2022Quote: ... then incubated in 1 mL of the secondary staining solution (goat anti-rabbit-AlexaFluor 488 [Thermofisher] at 1:1000 for Al1 in pupae and 1:3000 for Al1 in embryos, Donkey anti-rat-AlexaFlour 555 [Thermofisher] at 1:1000 for Al2 in Pupae and 1:3000 for Al2 in embryos and Hoechst 33342 at 1:1000 [Thermofisher] in blocking buffer) ...
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bioRxiv - Microbiology 2022Quote: ... then incubated with secondary IgG (goat anti-mouse Alexa546, goat anti-rabbit Alexa633, goat anti-rat, all 1:500, ThermoFisher) in PBS containing 0.5% Triton X-100 for 2 hours and counterstained with Hoechst 33342 (1 μg ml-1 ...
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bioRxiv - Neuroscience 2022Quote: ... Rat embryonic primary hippocampal cell cultures were prepared and transfected using Lipofectamine 2000 (Thermo Fisher Scientific, Invitrogen, catalog no. 11668019) as described previously (Janusz et al. ...
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Kynurenic acid promotes A1 to A2 astrocyte conversion against HIV-associated neurocognitive disorderbioRxiv - Microbiology 2022Quote: ... cortical astrocytes were dissected from SD neonatal rats (1-3 days) with the aseptic operation and digested by 0.25% trypsin (Gibco, USA) at 37°C for 20 min ...
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bioRxiv - Neuroscience 2022Quote: ... Rat: anti-SOX2 (Thermo, 14-9811-82 1:500) Other: Streptavidin-Alexa Fluor 594 conjugate (Thermo Scientific, S11227, 1:500).
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bioRxiv - Bioengineering 2022Quote: ... or transwell inserts precoated using 0.15 mg/mL rat collagen I in a humidified 5% CO2 incubator at 37 ± 0.5°C (Isotemp, Thermo Fisher Scientific). The cells were cultured in complete growth medium composed of endothelial cell basal medium (EBM-2 ...
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bioRxiv - Neuroscience 2024Quote: ... mice and rats were heavily anesthetized with ∼1.0-2.0 ml of urethane (Fisher Scientific, CA#AC325540500, 30% w/v, i.p.) and transcardially perfused with 120 ml of 1% PB followed by 180 ml of 4% granular PFA in PB ...
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bioRxiv - Genetics 2023Quote: The brains of E13.5 rats were lysed and 30 μg of protein combined with 5 μl 4x LDS Sample Buffer (ThermoFisher, #NP0004) and 2 μl 10x Sample Reducing Agent (ThermoFisher ...
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bioRxiv - Neuroscience 2023Quote: ... Cortices from embryonic day 18 Sprague Dawley rat were dissociated enzymatically in trypsin supplemented with 0.25% EDTA (Thermo Fisher Scientific) and physically by trituration ...
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bioRxiv - Neuroscience 2023Quote: ... No: A36511) were thawed and co-cultured with 15% primary rat cortical astrocytes (Cat. No: N7745100) both obtained from ThermoFisher Scientific (US) ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were seeded at the final density of 2×106 cells/ml in the central chamber of the DAX-1 chips embedded in a matrix composed of type I rat tail collagen at the final concentration of 2.3 mg/ml (Thermofisher, #A1048301). Each chip can be loaded with 10µl of gel ...
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bioRxiv - Neuroscience 2023Quote: ... The following siRNAs were used for knockdown of adult rat-derived hippocampal NSCs: Stealth RNAi™ siRNA Derl1-MSS289837 (Invitrogen), Stealth RNAi™ siRNA Stat5b-RSS332572 (Invitrogen) ...
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bioRxiv - Immunology 2023Quote: ... The cells were then blocked at 4℃ for 15 mins in 200 µl CyFACS buffer containing 3 µl rat serum (Invitrogen), 3 µl mouse serum (Invitrogen) ...
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bioRxiv - Molecular Biology 2023Quote: ... The hydrogels were washed several times under sterile conditions and incubated with 3.3% of Rat Tail Collagen 1 (Gibco A10483) solution at 4°C for 2 hours ...
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bioRxiv - Cell Biology 2022Quote: ... The sections were washed three times with PBS + 0.05 % Tween 20 and stained with anti-rat AF564 (1:100, Invitrogen, A11007) and SYTOX™ Green (1:1000 ...
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bioRxiv - Cell Biology 2023Quote: Primary cortical neurons were dissociated from brains of E18 Sprague-Dawley rat embryos or E16 C57Bl/6 mouse embryos and cultured in Neurobasal medium (Gibco) as previously described (Gopal et al. ...
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bioRxiv - Biochemistry 2023Quote: ... the reaction was started by the addition of 13 μL of microsome mix from the liver of Sprague−Dawley rats (Invitrogen; 20 mg protein/mL in 0.1 M phosphate buffer ...
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bioRxiv - Developmental Biology 2023Quote: ... Rat ES cells derived from SD rats’ blastocyst were cultured on feeder cells with N2B27 plus 2il: Mix DMEM/F12 (Gibco) with Neurobasal medium (Gibco ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... the reaction started upon the addition of 13 μL of microsome mix from the liver of Sprague-Dawley rats (Invitrogen; 20 mg protein/mL in 0.1 M phosphate buffer ...
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bioRxiv - Cell Biology 2023Quote: ... followed by additional 15 min incubation with 10 μg/ml Alexa Fluor 647-conjugated goat anti-rat IgG (cat# A-21247, Invitrogen) or goat anti-mouse IgG (cat # A-21235 ...
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bioRxiv - Immunology 2023Quote: ... and next for 30 min at 37 °C in the presence of chicken anti-rat IgG conjugated with Alexa Fluor 647 (Invitrogen) to stain CD14/IgG on the cell surface and allow its subsequent uptake ...
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bioRxiv - Biochemistry 2023Quote: ... the reaction was started by the addition of 13 μL of microsome mix from the liver of Sprague−Dawley rats (Invitrogen; 20 mg protein/mL in 0.1 M phosphate buffer ...
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bioRxiv - Biochemistry 2023Quote: ... the reaction was started by the addition of 13 μL of microsome mix from the liver of Sprague−Dawley rats (Invitrogen; 20 mg protein/mL in 0.1 M phosphate buffer ...
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bioRxiv - Developmental Biology 2023Quote: Protein from rat and human fetal lung explants was isolated by incubation with cell extraction buffer (ThermoFisher Scientific, Waltham, MA) supplemented with protease inhibitors (Sigma Aldrich ...
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bioRxiv - Developmental Biology 2023Quote: ... Embryos were suspended in a solution of DMEM without phenol red containing 10% rat serum and 1% low-melt agarose (Invitrogen) in a glass capillary tube ...
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bioRxiv - Neuroscience 2023Quote: ... Hippocampal neurons were prepared from rat E18 embryos and maintained in Neurobasal Medium supplemented with B-27 and 2 mM L-glutamine (Invitrogen) for 18-21 days in vitro (DIV).
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bioRxiv - Neuroscience 2023Quote: ... a thin layer of collagen matrix was applied on each coverslip (500µL of 3mg/mL collagen I (from rat tail, Thermo Scientific), 20µL of 1mg/mL laminin (Sigma-Aldrich) ...
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bioRxiv - Bioengineering 2024Quote: ... hBECs were grown in tissue culture flasks and plates pre-coated using 0.15 mg/mL rat collagen I in a humidified 5% CO2 incubator at 37 ± 0.5°C (Isotemp, Thermo Fisher Scientific). The cells were cultured in complete growth medium composed of endothelial cell basal medium supplemented with 5% fetal bovine serum ...
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bioRxiv - Neuroscience 2023Quote: ... RRID:AB_2877597) and Goat anti-Rat IgG (H+L) Alexa Fluor 488-conjugated secondary Ab (1:200, Invitrogen, Cat# A11006, RRID:AB_2534074) were used ...
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bioRxiv - Immunology 2024Quote: Total IgG from rat serum was purified on drip columns packed with Pierce Protein G agarose resin (Thermo Fisher Scientific). Pierce protein G IgG binding buffer (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... that was manually coated with 50 ug/mL rat tail collagen I (Ref. no. A10483-01, Gibco, Grand Island, NY) prior to cell plating ...
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bioRxiv - Immunology 2024Quote: ... Cells were then isolated as CD4 or CD8 respectively with immunomagnetic beads (Dynabeads Mouse CD4 +, Invitrogen, catalog: 11445D; or Dynabeads Sheep anti-Rat IgG, Invitrogen, catalog ...
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bioRxiv - Bioengineering 2024Quote: ... the polymerization of EDOT took place on the rat sciatic nerve constructs using ferric chloride (FeCl3) (Thermo Scientific #Cat: 012357.09) oxidant in the presence of ethanol ...
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bioRxiv - Bioengineering 2024Quote: hCMEC/D3 BECs were maintained in tissue culture flasks or multi-well plates pre-coated with 0.15 mg/mL rat collagen I in a humidified 5% CO2 incubator at 37 ± 0.5°C (Isotemp, Thermo Fisher Scientific). The cells were cultured in complete growth medium comprised of endothelial cell basal medium (EBM-2 ...
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bioRxiv - Neuroscience 2024Quote: ... tissues were washed 3x for 1 hr in adult brain wash solution and incubated overnight at 4°C or for 4 hr at room temperature with AlexaFluor488 anti-rat (1:250) (Invitrogen) and DAPI (1:250 ...
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bioRxiv - Biochemistry 2024Quote: ... 10 raw files were imported into Proteome Discoverer software for library construction using Swiss-iRT and Uniprot-Rat databases (version 2.0, Thermo Scientific). In the sucrose group ...
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bioRxiv - Neuroscience 2024Quote: ... The following primary antibodies were diluted in 10% NDS in PBTx and applied overnight: Rabbit anti-Iba1 (1:1,000; Wako; RRID: AB_839504) and Rat anti-GFAP (1:500; Invitrogen; RRID: AB_2532994). The next day ...
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bioRxiv - Neuroscience 2024Quote: ... or anti-mouse IgG (1:300; A21202) or anti-rabbit IgG (1:500; A21206) or anti-rat IgG (1:500; A21208, both Invitrogen), Alexa Fluor 594-labeled donkey anti-goat IgG (1:300 ...
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bioRxiv - Molecular Biology 2019Quote: ... 6-12 µg anti-HA antibodies (i.e., 12CA5, University of Chicago Monoclonal Antibody Facility) or 6 µg anti-V5 antibodies (R960-25, Invitrogen) per reaction were first conjugated to protein A sepharose beads in a 50% slurry that was first washed in IPP150 ...
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bioRxiv - Neuroscience 2021Quote: ... 1:1,000] and secondary antibodies (horseradish peroxidase-conjugated anti-rabbit antibody [Cytiva, 1:2,500], Alexa Fluor 488-conjugated anti-rabbit antibody [Thermofisher, 1:2,500] ...
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bioRxiv - Microbiology 2020Quote: ... Slides were washed with PBS and incubated for four hours with anti β-actin antibody labelled with 594 fluorophore using the Zenon Mouse IgG Labeling Kit (Thermo Fisher Scientific). Dilutions of primary and secondary antibodies were the same as the ones used for the staining of human cells described above ...
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bioRxiv - Microbiology 2019Quote: Pre-inoculated rabbit antisera and anti-HSP40 were coupled to magnetic beads using the DynabeadsTM Antibody Coupling Kit as per the manufacturers protocol (Thermo Fisher Scientific, 14311D). Parasite pellets were harvested from RBCs using 0.1% saponin ...
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bioRxiv - Cancer Biology 2020Quote: ... The membranes were probed with species-specific donkey anti-rabbit or goat anti-mouse HRP-conjugated secondary antibody and detected with enhanced chemiluminescence (ECL) detection kit (Thermo Fisher, Carlsbad, CA).
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bioRxiv - Microbiology 2021Quote: ... Unstained cell samples from each donor and compensation beads from the Invitrogen™ AbC™ Total Antibody Compensation Bead Kit (A10497; Thermo Fisher) were used to set voltages and create single stain controls ...
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bioRxiv - Cell Biology 2021Quote: ... antibody was used for immunoprecipitation of BRCA1 complexes from 2 mg of nuclear extract using Pierce Classic IP Kit (Thermo Fisher Scientific, 26146) according to manufacturer’s instructions.
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bioRxiv - Neuroscience 2022Quote: ... Kit (BMK-2202) and the primary antibody was detected with Alexa-594 conjugated goat anti-mouse IgG (Invitrogen A-11005, 1:1000 dilution). Alternatively ...
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bioRxiv - Neuroscience 2022Quote: ... Membrane was washed with 1X TBST and incubated with respective HRP conjugated secondary antibody for two hours and washed before developing the blots using chemilumiscence kit (Thermo Fisher Scientific, US). Details of all the primary and secondary antibodies used for western blot is provided in Supplementary Table 2.