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Citations for Thermo Fisher :
3001 - 3050 of 10000+ citations for SARS CoV 2 Spike Glycoprotein S1 Sheep Fc Tag HEK293 Biotin since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... The resulting constructs were transfected into HEK293 cells using the Lipofectamine 3000 Reagent (Invitrogen, Cat#L3000001) according to the manufacturer’s instructions ...
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bioRxiv - Physiology 2022Quote: ... The sample was subsequently labeled with the TAMRA-alkyne dye by using the Click-It™ TAMRA Glycoprotein Detection Kit (Thermo Fisher Scientific, Waltham, MA). The mixture was vortexed for 5 seconds after the addition of each component according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... and transfected with the respective pCDNA3.1+/MERS-CoV S construct using Lipofectamine 2000 (Invitrogen). Transfections were performed according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2020Quote: ... BM endothelial cells were immunopurified from cell suspensions using sheep anti-rat IgG Dynabeads (ThermoFisher Scientific 11035) pre-captured with a CD31 antibody (MEC13.3 ...
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bioRxiv - Molecular Biology 2021Quote: ... at 37°C without aeration or in THY agar medium supplemented with 5% sheep blood (Thermo Scientific) at 37°C in a 5% CO2 atmosphere ...
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bioRxiv - Microbiology 2021Quote: Hemolytic index was measured using 1.5% BHI agar plates supplemented with 5% defibrinated sheep blood (Thermo Scientific). After overnight incubation at 30°C ...
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bioRxiv - Molecular Biology 2019Quote: ... chromatin was incubated overnight with 3 µg of antibody coupled to Dynabeads Sheep Anti-Rabbit IgG (Invitrogen), in FA-buffer (50 mM HEPES/KOH pH7.5 ...
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bioRxiv - Microbiology 2021Quote: ... pneumoniae (one colony per species per plate) were further sub-cultured on Sheep Blood Agar plates (ThermoFisher) and species identity confirmed by matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS ...
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bioRxiv - Molecular Biology 2021Quote: ... the cells were incubated using a secondary antibody donkey anti-sheep Alexa Fluor 647™ (ThermoFisher, #A21448) 1:200 in FACS buffer for 45 min at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... Transformants were selected by plating inside Columbia agar supplemented with 3% defibrinated sheep blood (CBA, Thermo Scientific) containing the appropriate antibiotic and incubated at 37°C in 5% CO2 overnight ...
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bioRxiv - Neuroscience 2019Quote: ... antibody/antigen mixture was incubated with Dynabeads M-280 sheep anti-rabbit IgG (Thermo Fisher Scientific, 11203D) or Dynabeads M-280 sheep anti-mouse IgG (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... Transformants were selected by plating inside Columbia agar supplemented with 3% of defibrinated sheep blood (Thermo Scientific), followed by CRISPR-mediated counter selection ...
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bioRxiv - Cancer Biology 2022Quote: ... Antibody/antigen complexes were recovered with Dynabeads M-280 sheep anti-rabbit IgG (Thermo Fisher Scientific, 11203D). Beads with DNA fragments were washed once with RIPA buffer (50 mM Tris-HCl ...
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bioRxiv - Neuroscience 2022Quote: ... Actual inocula were determined by plating serial dilutions on sheep blood agar plates (Thermo Fisher, Waltham, MA). Age-and sex-matched 20–24-week-old ...
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bioRxiv - Molecular Biology 2024Quote: ... the membranes were incubated with donkey anti-sheep IgG HRP-conjugated (1:15,000 dilution; Thermo Fisher Scientific) for one hour at RT ...
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bioRxiv - Molecular Biology 2024Quote: ... Samples were then incubated with pre-washed sheep anti-mouse antibody (M280)-conjugated Dynabeads (Thermo Fisher, 11201D) for 1 h at room temperature ...
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bioRxiv - Molecular Biology 2023Quote: The chromatin immunoprecipitation experiments were performed using magnetic beads Dynabeads M-280 sheep anti-mouse IgG (Invitrogen), mixed with an anti 9E10 C-myc antibody (Santa Cruz) ...
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bioRxiv - Microbiology 2023Quote: ... Alexa Fluor 488 donkey anti-mouse (A21202) and Alexa Fluor 488 donkey anti-sheep (A11015) from Invitrogen/ThermoFisher Scientific ...
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bioRxiv - Physiology 2023Quote: ... Chromatin complexes were precipitated with 100µl Dynabeads M-280 (sheep anti-rabbit #11203D; Thermo Scientific, Waltham, MA). After washes and elution ...
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bioRxiv - Cell Biology 2023Quote: ... Secondary antibodies used were Alexa Fluor 488 donkey anti-sheep at 1:500 (Invitrogen, Carlsbad, CA, USA), Alexa Fluor 488 goat anti- mouse at 1:500 (Invitrogen ...
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bioRxiv - Neuroscience 2024Quote: ... and 647 (1:500 for all; PIA32773-mouse; A21202-mouse; PIA32731TR-rabbit; PIA32794-rabbit; A21448-sheep, Invitrogen). The sections were examined using an AX-R Confocal Microscope coupled to a fully automated Ti2-E system (Nikon Instruments Inc ...
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bioRxiv - Microbiology 2024Quote: ... was purchased and prepared according to the instructions with 6% (v/v) defibrinated sheep blood (Thermo Scientific) added ...
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bioRxiv - Molecular Biology 2021Quote: ... Resulting Pol IV-RDR2 dsRNAs were then subjected to the indicated amounts of S1 nuclease (Thermo Fisher) digestion for 10 min at 37°C using the manufacturer supplied S1 nuclease digestion buffer ...
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bioRxiv - Developmental Biology 2019Quote: ... the cells were incubated with secondary antibodies (Table S1) and Alexa Fluor 488 Phalloidin (Thermo Fisher Scientific). Then the cells were washed ...
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bioRxiv - Immunology 2022Quote: ... and the primers listed in table S1 on a ViiA 7 Real-Time PCR System (Applied Biosystems) with the following cycling parameters ...
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bioRxiv - Immunology 2021Quote: ... Chromatin was sonicated and immunoprecipitated using antibodies listed in Table S1 and captured on Dynabeads (ThermoFisher Scientific). Immunoprecipitated protein-DNA complexes were reverse cross-linked and chromatin DNA purified as described 24 ...
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bioRxiv - Cell Biology 2021Quote: ... Myoblasts were transfected with Srf and Acta siRNAs (Dharmacon; see Table S1) using lipofectamine RNAiMAX (ThermoFisher Scientific) at day 0 and day 2 of the differentiation process ...
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bioRxiv - Microbiology 2020Quote: ... Gene expression was measured by qRT-PCR using gene-specific primers (Table S1) and PowerSYBR Green (Invitrogen). Samples were run in triplicate and normalized to an S7 reference gene and quantified using the 2−ΔΔCT method as previously 33.
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bioRxiv - Molecular Biology 2022Quote: ... Yeast strains used in Tables S1-S2-S3 were taken from the heterozygote yeast deletion collection (ThermoFisher). For nicotinamide (NAM ...
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bioRxiv - Genomics 2023Quote: ... and the DNA quality was checked using the NanoDrop 2000 Spectrophotometer (ThermoFisher Scientific, Waltham, USA; Text S1). The DNA length and RNA absence were assessed by 2% agarose gel electrophoresis (50 V ...
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bioRxiv - Genomics 2023Quote: ... Whole liver tissue was homogenized using the Polytron PowerGen 500 S1 tissue homogenizer (Fisher Scientific, Hampton, NH) in Radioimmunoprecipitation assay buffer (LifeTechnologies ...
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bioRxiv - Microbiology 2024Quote: ... parviporum strains (Supplemental Table S1) were treated with the TURBO DNA-free™ Kit (Thermo Fisher Scientific) and sent to SEQme s.r.o ...
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bioRxiv - Cell Biology 2019Quote: ... mouse monoclonal antibody to Xpress tag (R910-25) were from ThermoFisher Scientific (Rockford ...
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bioRxiv - Genomics 2020Quote: ... as instructed using antibodies against HA-tag (Thermo Fisher cat#26183), MYC-tag (Abcam ...
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bioRxiv - Molecular Biology 2020Quote: ... and mouse monoclonal anti-HA Tag (ThermoFisher Scientific 26183, 1:10,000), used with either IRDve® 680LT anti-mouse (LI-COR ...
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bioRxiv - Biochemistry 2019Quote: ... MCM3 was cloned with an N-terminal FLAG tag (DYKDDDDK, ThermoFisher). The coding sequence for MCM2 was cloned into QB3 Macrolab vector 4C to generate an N-terminal His6-MBP fusion ...
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bioRxiv - Cancer Biology 2019Quote: ... 1:500 DYKDDDDK Tag Polyclonal Antibody (ThermoFisher Scientific, cat#PA1-984B), 1:1000 Goat anti-Rabbit IgG (H+L ...
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bioRxiv - Biochemistry 2020Quote: ... The membrane was incubated with GST-TAG antibody (1:1000, ThermoFisher) or anti-MC4 (rabbit ...
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bioRxiv - Cell Biology 2021Quote: ... the grids were incubated with anti-HA tag antibody (Invitrogen, 5B1D10), 10X diluted in 1% BSA in PBS for 3 h at room temperature ...
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bioRxiv - Molecular Biology 2020Quote: ... Phosphopeptides were labeled with Tandem Mass Tag (TMT) (Thermo Fisher Scientific), desalted using SDB-XC StageTips ...
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bioRxiv - Microbiology 2019Quote: ... V5 was detected with mouse anti-V5 tag monoclonal antibody (Invitrogen), and c-Myc was detected with rabbit monoclonal anti-c-Myc antibody Y69 (Abcam) ...
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A Bidirectional Switch in the Shank3 Phosphorylation State Biases Synapses toward Up or Down ScalingbioRxiv - Neuroscience 2021Quote: ... Dried Tandem Mass Tag (TMT) 10-plex reagent (Thermo Fisher Scientific) was reconstituted at 20 µg/µl in 100% anhydrous MeCN and added to samples at a 1:1 TMT to peptide mass ratio ...
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bioRxiv - Molecular Biology 2022Quote: ... Tandem Mass Tag (TMT)11plex Isobaric Label Reagent Set (ThermoFisher Scientific) was utilized for this application ...
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bioRxiv - Plant Biology 2022Quote: ... benthamiana was prebound to Strep-tag®II beads (Thermo Scientific) for two hours under rotation at 4 °C ...
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bioRxiv - Microbiology 2021Quote: ... 5x-His mouse anti-tag was used at 1:500 (Invitrogen). Anti-mouse HRP secondary antibody was used at 1:10,000 (Invitrogen) ...
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bioRxiv - Microbiology 2021Quote: ... or HRP V5 tag monoclonal antibody (1:1000, Invitrogen, # R961-25) were diluted in 0.05% PBST and incubated with the blots for 1 h at room temperature or 4 °C overnight ...
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bioRxiv - Molecular Biology 2020Quote: ... The following commercial antibodies were used: TAP tag (ThermoFisher Scientific #PICAB1001), Rpb1 (8WG16 ...
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bioRxiv - Genomics 2019Quote: ... normalized and labeled with tandem mass tags (TMT-10plex, ThermoFisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2019Quote: ... and labeled with 10-plex tandem mass tags (Thermo Fisher Scientific) at ambient temperature with 600 rpm shaking for 2 hr ...
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bioRxiv - Cell Biology 2020Quote: ... Tryptic peptides were then labeled with TMT 10pplex isobaric tags (ThermoFisher REF# 90110 ...