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Citations for Thermo Fisher :
3251 - 3300 of 10000+ citations for SARS CoV 2 Spike Glycoprotein S1 Sheep Fc Tag HEK293 Biotin since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... Cells transduced with Lenti-Spike were treated with 5 µg/ml Hoechst solution (Molecular Probes®) before being analyzed under a microscope ...
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bioRxiv - Microbiology 2024Quote: ... Spike sequences were cloned into a phCMV backbone (GenBank: AJ318514) using Gateway cloning (Thermo Fisher Scientific) or restriction enzyme digestion followed by ligation with T4 DNA ligase (New England Biolabs) ...
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bioRxiv - Microbiology 2020Quote: ... HEK293 cells were transfected with pCMV-AT1R-IRES-Puro gene using Lipofectamine 3000 reagent (Thermo Fisher Scientifics). Approximately 24 hours posttransfection ...
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bioRxiv - Immunology 2021Quote: HEK293 and RAW cells were transfected using Lipofectamine 3000 or Lipofectamine LTX Transfection Reagent (Life Technologies, # L3000015) according to the manufacturer’s protocol.
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bioRxiv - Neuroscience 2020Quote: Total RNA from HEK293 cells and human brain prefrontal cortex were isolated with the TRIzol reagent (Ambion) according to the manufacturer’s recommendations ...
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bioRxiv - Cancer Biology 2020Quote: ... not commercially obtained but can be provided on request) (42) and HEK293-FT (R70007, Thermo Fisher Scientific) cells were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM ...
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bioRxiv - Physiology 2021Quote: ... The expression vector was co-transfected with pOG44 into Flp-In T-REx HEK293 cells (Life Technologies), in accordance with the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2021Quote: Cells were cultured in DMEM (HEK293, Neuro-2a) or RPMI 1640 (SH-SY5Y) with 10% FBS (Gibco). Cells grew on petri dishes to a density of 1×105/cm2 ...
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bioRxiv - Biochemistry 2020Quote: Immunoprecipitation from HEK293 cell lysates was conducted using Dynabeads protein G magnetic beads (Invitrogen, Thermo Fisher Scientific) according to manufacturer’s protocol with a following modification ...
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bioRxiv - Biochemistry 2020Quote: Immunoprecipitation from HEK293 cell lysates was conducted using Dynabeads protein G magnetic beads (Invitrogen, Thermo Fisher Scientific) according to manufacturer’s protocol with a following modification ...
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bioRxiv - Biochemistry 2022Quote: ... HEK293-PSMB2-HB cells were transfected with 5 μg of the cloned plasmid using Lipofectamine 3000 (Invitrogen). After 48 h incubation ...
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bioRxiv - Cell Biology 2022Quote: ... HEK293 Jump-In and Flp-In TREx cells that allow doxycycline-dependent transgene expression were from Invitrogen. Cell lines were checked for mycoplasma infection by PCR or ELISA ...
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bioRxiv - Biochemistry 2021Quote: ... HEK293-based EGFP reporter and Hela cells were maintained in Dulbecco’s Modified Eagle’s medium (DMEM, ThermoFisher Scientific) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Immunology 2021Quote: ... growth media containing DMEM (HEK293) or DMEM/F12 (CHO) (both high glucose, pyruvate, Thermo Fisher Scientific (TFS)) supplemented with 10% (v/v ...
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bioRxiv - Neuroscience 2021Quote: ... HEK293S GnTI- cells were grown at 37°C with 8% carbon dioxide in Freestyle 293 medium (Gibco) supplemented with 2% (v/v ...
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bioRxiv - Neuroscience 2020Quote: ... Transfected HEK293 cells were solubilized in 0.1-1% (w/v) n-dodecyl-β-D-maltoside (ThermoFisher Scientific), 1 mM phenylmethylsulfonyl fluoride (Sigma-Aldrich) ...
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bioRxiv - Neuroscience 2022Quote: ... and 5% CO2 in DMEM (for HEK293 and HeLa) supplemented with 10% Fetal bovine serum (Invitrogen, Switzerland) and 1% of Penicillin/Streptomycin (Invitrogen ...
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bioRxiv - Biochemistry 2020Quote: HEK293 cells grown to 30-40% confluence in 6-well dishes were transfected with Lipofectamine 2000 (Invitrogen) in 35mm plates with 0.5 μg of pLKO shRNA or pLVX expression plasmids (Takara) ...
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bioRxiv - Cell Biology 2020Quote: Hek293 cells (ATTC® CRL-1573) were grown in DMEM + GlutaMAX™-I (Gibco, Invitrogen, Paisley, UK) with 10% fetal bovine serum (Cornig #35-010 ...
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bioRxiv - Cell Biology 2020Quote: Hek293 cells (ATTC® CRL-1573) were grown in DMEM + GlutaMAX™-I (Gibco, Invitrogen, Paisley, UK) with 10% fetal bovine serum (Cornig #35-010 ...
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bioRxiv - Synthetic Biology 2019Quote: Luc14 and Gal4-EED/luc HEK293 cells were grown in Gibco DMEM high glucose 1× (Life Technologies) with 10% Tet-free Fetal Bovine Serum (FBS ...
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bioRxiv - Neuroscience 2019Quote: ... The inter-molecular tau FRET biosensor was generated by transiently transfecting HEK293 cells using Lipofectamine 3000 (Invitrogen) with tau-GFP and tau-RFP (1:20 DNA plasmid concentration ratio) ...
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bioRxiv - Molecular Biology 2021Quote: ... were transfected into HEK293 T-Rex cells with Lipofectamine CRISPRMAX Cas9 Transfection Reagent (Thermo Fisher Scientific, CMAX00008). After 48 h of incubation ...
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bioRxiv - Molecular Biology 2021Quote: HEK293 stable cells overexpressing Flag-INTS118 and Flag-Ago215 were cultured in DMEM media (Gibco, #11965-084) containing puromycin and supplemented with 10% FBS (Atlas Biologicals ...
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bioRxiv - Cancer Biology 2019Quote: ... Both HEK293 and HEK293T cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) (Gibco/Invitrogen, Inc., USA) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Cancer Biology 2019Quote: ... Both HEK293 and HEK293T cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) (Gibco/Invitrogen, Inc., USA) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Biochemistry 2020Quote: Human Embryonic Kidney 293 cells (HEK293, ATCC CRL-1573) were grown under standard conditions in DMEM (Gibco) supplemented with 10% FBS (BioConcept) ...
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bioRxiv - Molecular Biology 2020Quote: Plasmids encoding RiboTag constructs were used to transiently transfect HEK293 cells with lipofectamine 3000 (Thermo Fisher Scientific) as recommended by the manufacturer ...
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bioRxiv - Cell Biology 2020Quote: ... HEK293 cells were cultured in Dulbecco’s modified Eagle’s medium supplemented with 10% fetal bovine serum (FBS; Invitrogen) and 200 U/ml of penicillin and streptomycin (Invitrogen) ...
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bioRxiv - Cell Biology 2021Quote: HEK293 cells were grown and maintained in DMEM/F12 medium (Gibco/Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 10% FCS (Eurobio ...
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bioRxiv - Cell Biology 2021Quote: Cells including MEFs and STIM1-KO HEK293 cells (Emrich et al., 2019) were maintained in DMEM (Invitrogen) supplemented with 10% FBS and 1% Penicillin/Streptomycin (Invitrogen) ...
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bioRxiv - Cancer Biology 2022Quote: HEK293 FT cells were transfected with dVPR and VSVG packaging plasmids using Lipofectamine 2000 reagent (ThermoFisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... TAP cell lines were generated based on the HEK293 Flp-In T-REx system (Thermo Fisher Scientific). HEK293 cell lines with tetracycline inducible TAP-tagged baits were generated by co-transfection of 0.1 µg of plasmid containing the TAP-tagged bait (hsINTS13ΔC ...
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bioRxiv - Neuroscience 2023Quote: The human embryonic kidney cells (HEK293 and HEK293T) were maintained in Dulbecco’s Modified Eagle Medium (DMEM, Gibco) supplemented with 15% Fetal Bovine Serum (FBS ...
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bioRxiv - Cell Biology 2023Quote: ... HEK293 cells were seeded in 10 cm2 dish with Dulbecco’s Modified Eagle Medium (#11965118, Thermo Fisher Scientific) supplemented with 25 mM HEPES ...
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bioRxiv - Bioengineering 2023Quote: ... HEK293 6E cells were used for transient production of these proteins following standard protocols for 293Fectin (Invitrogen). The cell cultures were harvested at 5 d post-transfection by centrifugation at 6,000 rpm for 15 min ...
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bioRxiv - Microbiology 2023Quote: ... HeLa-FRT UBAP2L KO and HEK293 cell lines were cultured in DMEM GlutaMax media (Thermo Fisher Scientific) at 37C in a humidified incubator with 5%CO2 ...
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bioRxiv - Molecular Biology 2023Quote: ... GFP-APEX2-NIK3 expressing U2OS (U2OS:GFP-APEX2-NIK3) and HEK293 cells were cultured in Dulbecco’s modified eagle’s medium (DMEM, Gibco) with 10% fetal bovine serum (FBS ...
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bioRxiv - Biophysics 2023Quote: Flp-In™ T-REx™ 293 cells (referred to as HEK293 through the manuscript; Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... 1.5ug of each plasmid was transfected independently into HEK293 cells using lipofectamine 3000 (Invitrogen, Carlsbad, CA, USA). Forty-eight hours after transfection ...
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bioRxiv - Cell Biology 2023Quote: ... and HEK293 (NT and FASTKD5 sgRNAs) samples were purified using the Dynabeads mRNA purification kit (ThermoFisher, 61006) according to the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2023Quote: ... We cultured the cells in Dulbecco’s modified Eagle’s medium (DMEM; Invitrogen; RAW 264.7, HEK293, and NIH-3T3) or F-12K medium (Caisson ...
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bioRxiv - Developmental Biology 2023Quote: HEK293 cells were transfected with 3 μg of linearised plasmid using Lipofectamine 3000 Transfection Reagent (Invitrogen, #L3000001) in wells of a 6-well plate ...
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bioRxiv - Molecular Biology 2023Quote: ... 500 μl of 4 × 104 cells/ml HEK293 Flp-In T-REx cells (Thermo Fisher Scientific, R78007) or HEK293 Flp-In T-REx SBP-eIF4A1 (Phe163Leu-Ile199Met ...
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bioRxiv - Molecular Biology 2024Quote: HEK293 LgBit cells (gift from Itzhaki lab) were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM, ThermoFisher, 41966052) supplemented with L-glutamine (Thermo Fisher ...
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bioRxiv - Biochemistry 2024Quote: Human neuroblastoma SH-SY5Y cells (ATCC) or HEK293 (ATCC) cells were cultured in DMEM/F12 media (Gibco) or DMEM High-glucose (Gibco ...
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bioRxiv - Microbiology 2021Quote: ... followed by incubation with anti-SARS-CoV2 nucleocapsid mouse mAb (MA5-29981, Invitrogen; 1:1000) overnight at 4°C ...
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bioRxiv - Systems Biology 2020Quote: ... Fragments were rocked on ice with crypt isolation buffer (10 mM EDTA, Life Technologies, in PBS0 supplemented with 500X dilution of Primocin, Invivogen, 10 mM HEPES, Life Technologies, and 2% FCS, Life Technologies) for 30 min ...
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bioRxiv - Cell Biology 2020Quote: ... Alexa Fluor donkey anti-sheep 488 secondary or Alexa Fluor donkey anti-rabbit 594 secondary (Thermo Fisher Scientific) diluted (1:500 ...
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bioRxiv - Molecular Biology 2021Quote: For each sample and IgG control: 120 μl of Dynabeads M-280 Sheep anti-mouse IgG (Thermo Fisher) were pre-blocked overnight at 4°C on a wheel in 1 ml of PBS supplemented with 0.5% BSA ...