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Citations for Thermo Fisher :
3001 - 3050 of 10000+ citations for 6 TRIFLUOROMETHYL 1 2 3 4 TETRAHYDRO ISOQUINOLINE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: Nucleotide sugars were separated using Inertsil ODS-4 250 × 4.6 mm column with a particle size 3 μm (GL Science) and Vanquish Flex HPLC system (Thermo Fisher Scientific), with 100 mM potassium phosphate buffer pH 6.4 containing 8 mM tetrabutylammonium hydrogen sulphate as buffer A and 70 % solution of buffer A in 30 % acetonitrile as buffer B ...
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bioRxiv - Cell Biology 2020Quote: ... A pool of 4 siRNAs targeting mouse SNAP-47 and control Luciferase siRNA (Target Sequence: 5’-CGTACGCGGAATACTTCGA-3’) (Dharmacon, Thermofisher Scientific) were electroporated along with GFP into E15.5 cortical neurons (2 µg GFP + 50 pmol siRNAs) ...
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bioRxiv - Cancer Biology 2021Quote: ... for 10 min at 70°C and separated on 4-12% Bis/Tris-gels or 3-8% Tris/Acetat-Gels (Thermo Fisher). After separation ...
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bioRxiv - Immunology 2021Quote: ... The final wash was followed by the addition of Nitro-blue Tetrazolium Chloride/5-bromo-4-chloro 3 ‘indolyl phosphate p-toludine salt (NBT/BCIP chromagen) substrate solution (Thermo Scientific) for 7 min ...
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bioRxiv - Cell Biology 2022Quote: ... 3.5 - 4 ml aliquots of the resulting 10,000xg supernatants were added up with 3-2.5 ml 1X PBS (Gibco, Carlsbad, CA, USA) to reach a final volume of 6.5 ml and were transferred on top of a 60% - 10% iodixanol gradient (Progen Biotechnik GmbH ...
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bioRxiv - Immunology 2022Quote: ... 700 μg of protein were mixed and rotated with 0.5 μg of STING antibody for 3 h at 4 °C after which 25 μL of Dynabeads Protein G (Invitrogen, 10004D) were added to each sample and rotated for an additional hour ...
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bioRxiv - Microbiology 2022Quote: ... After 3 hours a fraction of the bacteria was fixed by the addition of 4% methanol-free paraformaldehyde (PFA, Thermo Scientific) for 10 min at room temperature and the reaction was quenched by the addition of 0.3 M glycine ...
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bioRxiv - Neuroscience 2022Quote: ... Electrophoretic run of samples containing 30μg of proteins was performed on precast NuPAGE 3-8% or 4-12% Tris-Acetate Protein Gels (Thermo Fisher Scientific), or on home-made acrylamide gels 8-15% ...
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bioRxiv - Neuroscience 2022Quote: ... 3 hours later cultures were washed and fixed with 4% paraformaldehyde (Electron Microscopy Solutions) in phosphate-buffered saline (PBS; Thermo Fisher) for 10 minutes ...
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bioRxiv - Neuroscience 2022Quote: ... Biotinylated glycoproteins were enriched by incubation for 3 hrs at 4°C on a rotating wheel with high affinity streptavidin agarose beads (ThermoFisher Scientific). Extensive washing was performed on a vacuum manifold ...
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bioRxiv - Molecular Biology 2022Quote: ... Then 3 μg of purified recombinant protein was added with 4 μl of RiboLock RNase Inhibitor (Thermo Fisher Scientific, Waltham, MA). The mixture was incubated at room temperature for 1 hour ...
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bioRxiv - Neuroscience 2022Quote: Mammary glands were harvested from lactating GCaMP6f;K5CreERT2 mice diced into 3- to 4-mm3 pieces and loaded with CellTracker Red (1.5 μM, ThermoFisher C34552, ThermoFisher, Waltham, MA) for at least 30 min in DMEM/F12 containing 10% FCS ...
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bioRxiv - Cell Biology 2022Quote: HSC cells atop ECM hydrogels subjected to the different treatments for 3 d were fixed with 4% paraformaldehyde (Thermo Fisher Scientific) at room temperature for 20 min ...
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bioRxiv - Developmental Biology 2022Quote: ... Epithelial sheets of embryos from the same litter were pooled (3-4 embryos per sample) and incubated for 10 min at RT in TrypLE Select Enzyme (Life Technologies/Gibco). Dissociated cells were resuspended in 0.5% BSA ...
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bioRxiv - Neuroscience 2023Quote: ... then incubated for 3 days (room temperature during the day and 4 °C at night) in PAT-NGS containing rabbit anti-GFP (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... BN-PAGE was performed at 4°C on Native PAGE Novex 3-12% Bis-Tris Protein gels (Thermo Fisher Scientific, BN1001) according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: The tadpole was stepwise rehydrated in 1x PBS with 0.1% Triton X-100 (PBST) and blocking was performed for 3 days at 4°C in 10% CAS-Block / 90% PBST (008120, Life Technologies). Staining was performed using Atp1a1 antibody (1:500 ...
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bioRxiv - Cell Biology 2023Quote: ... digitonin-solubilized (4 g/g) mitochondria (10–50 μg) were loaded in NativePAGE Novex 3–12% (vol/vol) gels (Life Technologies). After electrophoresis ...
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bioRxiv - Microbiology 2023Quote: ... the indicated amounts of protein sample were boiled at 95°C for 3 min and separated by 4–12% Bis-Tris SDS-PAGE (Thermo Scientific) either in 1x MES buffer (Thermo Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... maintained at 37° C and 5% CO2 and passaged every 3-4 days using trypsin-EDTA 0.05% (Life Technologies, Paisley, UK) to detach the cells ...
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bioRxiv - Neuroscience 2023Quote: ... cells were fixed with 4% PFA for 20 min at RT and then washed (3×) in DPBS with calcium and magnesium (DPBS +/+, Gibco Invitrogen). For structural proteins ...
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bioRxiv - Immunology 2023Quote: ... Eight days after subcutaneous immunisation of C57BL/6 mice with 50 μg of 4-Hydroxy-3-nitrophenyl acetyl hapten conjugated chicken gamma globulin (NP-CGG) in Imject Alum Adjuvant (ThermoFisher Scientific), 4.3 µg MAR hypoxia detecting probe (Goryo Chemical ...
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bioRxiv - Physiology 2024Quote: ... plus 1% penicillin/streptomycin in a humidified incubator at 37 °C and 5% CO2 and passaged every 3-4 days using 0.05% of Trypsin-EDTA (Thermo Fisher Scientific) to lift the cells from the culture dish ...
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bioRxiv - Molecular Biology 2023Quote: ... and blotted on two sides for 3 sec at 4 °C and 100% humidity before vitrified in liquid ethane using a Vitrobot Mark IV (Thermo Fisher). The grids were subsequently transferred to a Titan Krios (G3i ...
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bioRxiv - Biochemistry 2024Quote: The sample was blotted for 3 s with blot force 20 at 100% relative humidity and 4 °C using a Vitrobot Mark IV (Thermo Scientific). Grids were plunge frozen in liquid ethane cooled by liquid nitrogen ...
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bioRxiv - Immunology 2021Quote: ... The supernatant was then nutated overnight at 4 °C with 1 ml of Protein G Sepharose 4 Fast Flow slurry (Invitrogen) per 25ml of supernatant ...
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bioRxiv - Neuroscience 2020Quote: ... free-floating coronal hippocampal slices of 50 µm thickness were produced and incubated overnight in staining solution (4% normal goat serum, 0.4% Triton-X 100 and 4% BSA) with anti-GFAP antibody (1:1000, ThermoFisher, rat) and anti-Iba1 antibody (1:1000 ...
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bioRxiv - Cell Biology 2021Quote: Handpicked islets (~180) from control (n=4) and diabetic (n=4) mice were placed into 1 mL Trizol (Cat# 15596018, Ambion) and processed for RNA extraction ...
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bioRxiv - Cancer Biology 2022Quote: ... supplemented with 10% (KCL-22, K562, REH, NB-4) or 20% (MOLT-4, OCI-AML3, MOLM-13, Kasumi-1) fetal bovine serum (Gibco) and 1% penicillin–streptomycin (Gibco ...
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bioRxiv - Neuroscience 2024Quote: ... tissues were washed 3x for 1 hr in adult brain wash solution and incubated overnight at 4°C or for 4 hr at room temperature with AlexaFluor488 anti-rat (1:250) (Invitrogen) and DAPI (1:250 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... were labeled in loading buffer (HBSS with 1% FBS, 1 mM CaCl2 and 1 mM MgCl2) containing 1 μM Fluo-4 AM (Thermo Fisher) with 0.02% Pluronic F-127 at 37 °C for 30 min with gentle agitation every 10 min ...
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bioRxiv - Cancer Biology 2021Quote: Relative cell proliferation rates were assayed using an MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay kit (Vybrant™ MTT Cell Proliferation Assay Kit, Invitrogen™ ...
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bioRxiv - Cancer Biology 2022Quote: ... 10 μL of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT, concentration 5 mg/mL, Invitrogen, cat. M6494) in PBS was added to each well containing culture medium and incubated for 2.5 h at 37 °C ...
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bioRxiv - Biochemistry 2019Quote: ... Peptides were desalted (Acclaim PepMap 100, 75µm × 2 cm, nano viper, C18, 3 µm, 100 Å, ThermoFisher, Waltham, MA) and separated (Acclaim ...
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bioRxiv - Cell Biology 2019Quote: ... cells were loaded with 2 µM CellEvent™ Caspase-3/7 Green Detection Reagent (Life Technologies, Carlsbad, CA, USA) according to the manufacturer’s protocol and visualized using FITC 488 nm filter ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... plates were treated with 2 μM of the CellEvent™Caspase-3/7 green detection reagent (Life Technologies, UK) for 60 minutes at 37 °C in the dark ...
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bioRxiv - Bioengineering 2021Quote: ... Medium was changed every 2-3 days and cell passages were carried out using a TrypLE Express solution (Gibco). Human TERT-immortalized gingival fibroblasts (hTERT-HGF ...
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bioRxiv - Neuroscience 2019Quote: ... Purified α-Syn was dialyzed in a 2 kDa Slide-A-Lyzer unit (Thermo Scientific, for max. 3 ml) against HPLC-water (VWR) ...
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bioRxiv - Neuroscience 2020Quote: ... pooled and purified cDNA libraries with mean sizes of 550-600 bp and concentrations of 2-3 ng/µl (measured with Qubit 2.0, Invitrogen) were sequenced on a HiSeq 2500 Illumina system with single-end 50 bp read lengths ...
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bioRxiv - Cell Biology 2021Quote: ... 2 and 3 (100 pmol for each) or control siRNA were transfected into HeLa cells using Lipofectamine 2000 (Invitrogen). 48 h after transfection ...
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bioRxiv - Microbiology 2021Quote: ... After incubation for 2 h at 37°C the medium was aspirated and 3 mL of fresh DMEM (Gibco) supplemented with 5% New Born Calf Serum (NBCS ...
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bioRxiv - Microbiology 2021Quote: ... The other half of the wells of the Caco-2 cells was subsequently incubated anaerobically for 3 h with 0.3% gentamicin (50 μg/ml, Gibco) to eliminate all extracellular L ...
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bioRxiv - Microbiology 2021Quote: ... The other half of the wells of the Caco-2 cells was subsequently incubated anaerobically for 3 h with 0.3% gentamicin (50 µg/ml, Gibco) to eliminate all extracellular L ...
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bioRxiv - Cell Biology 2021Quote: ... under 5% CO2 at 37°C in humidifying conditions and passaged every 2-3 days using 0.25% Trypsin-EDTA (25200056, Gibco). Cells were routinely tested for mycoplasma ...
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bioRxiv - Cancer Biology 2021Quote: Relative cell proliferation rates were determined using an MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay kit (Vybrant™ MTT Cell Proliferation Assay Kit, Invitrogen™ ...
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bioRxiv - Microbiology 2022Quote: ... A total of 3 µg of RNA was DNAse treated using 2 units of Turbo DNase I enzyme (Invitrogen). A total of 1 µg of DNAse-treated RNA was reverse transcribed using Superscript III reverse transcriptase (Invitrogen/Thermo Fisher) ...
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bioRxiv - Molecular Biology 2022Quote: ... Cell viability was determined by the MTT [3-(4,5-di-methylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] (Invitrogen, Waltham, MA) reduction assay ...
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bioRxiv - Neuroscience 2021Quote: ... The culture medium was changed every 2 to 3 days and the cells were split every 5 to 7 days using 0.25% Trypsin-EDTA (Gibco), up to 20 times ...
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bioRxiv - Cancer Biology 2021Quote: ... conditioned media was replaced by a 1.2 mM 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT, Thermo Fisher Scientific) solution ...
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bioRxiv - Immunology 2020Quote: ... 1×106 cells per condition were plated in 96 U-shape well plates (CELLSTAR, Kremsmünster) and rested for 2-3 h in RPMI (Gibco), supplemented with 10% FBS ...