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Citations for Thermo Fisher :
2801 - 2850 of 10000+ citations for 6 TRIFLUOROMETHYL 1 2 3 4 TETRAHYDRO ISOQUINOLINE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... 2 dpf zebrafish larvae were dechorionated and fixed with 4% formaldehyde methanol-free (Pierce™ Thermofisher, #28906) in BT buffer (1.0g sucrose ...
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bioRxiv - Developmental Biology 2023Quote: ... 2021 Total RNA was extracted from 2-4 day old female ovaries using the MirVana kit (Ambion). rRNA was depleted using antisense rRNA oligo hybridization with subsequent RNase H digestion ...
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bioRxiv - Cell Biology 2023Quote: ... followed by 2 hour incubation at 4℃ with 50 µL of magnetic Dynabeads Protein G (Invitrogen, #10004D). Beads were washed with 1x TBS Tween-20 washing buffer (Thermo Scientific ...
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bioRxiv - Microbiology 2024Quote: ... GECs (8 x 104 cells) were seeded in 4-well chamber slides (Fisher Scientific# 12-565-2) and pre-treated with 2 mM Glu-Glu for 1 h prior to infection with wild-type or ΔgdhA strains of P ...
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bioRxiv - Microbiology 2019Quote: ... after which time 1 μL FM 4-64 (Life Technologies, 1 μg/μL in DMSO) was added to the suspension and gently mixed ...
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bioRxiv - Neuroscience 2022Quote: ... probenecid (1:250) and PowerLoad concentrate (1:100) (Fluo-4 Calcium Imaging Kit, Molecular Probes) in BrainPhys basal media for 30 min (37 °C ...
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bioRxiv - Bioengineering 2019Quote: ... denaturing gels were run with 6 μL per well of a 1:1 mixture of sample and Gel Loading Buffer II with formamide (Ambion) in a 10% TBE-Urea ...
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bioRxiv - Physiology 2021Quote: ... we incubated the slices for 50 minutes at RT in the dye-loading solution (6 μM Oregon Green 488 BAPTA-1 AM (OGB-1, Invitrogen), 0.03% Pluronic F-127 (w/v) ...
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bioRxiv - Physiology 2022Quote: ... The prepared slices were stained in the dye-loading solution (6 μM Oregon Green 488 BAPTA-1 AM (OGB-1, Invitrogen), 0.03 % Pluronic F-127 (w/v) ...
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bioRxiv - Microbiology 2021Quote: ... The freeze-dried material was dissolved in 300 μl of 6:1 (v/v) of methyl sulphoxide D6 (99.9% atom D + 1% tetramethylsilane, ACROS Organics):D2O (100% atom D ...
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bioRxiv - Neuroscience 2022Quote: ... embryoid bodies were embedded in 15 µL Matrigel droplets and cultured in 6 well plates containing c-organoid differentiation media consisting of 1:1 DMEM-F12 and Neurobasal media (Gibco), with addition of 0.5% N2 supplement (Life Technologies) ...
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bioRxiv - Cancer Biology 2023Quote: ... per 1×104 SiHa and 1×103 HeLa cells/6-well culture dish were transfected with Lipofectamine RNAi-Max (Invitrogen). Efficient knock down was confirmed by western blot analysis ...
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bioRxiv - Cell Biology 2020Quote: ... 2% BSA and 2 mM EDTA at 1 x 106 cells per 100 μl: anti-ITGA7 APC 1:500 (Invitrogen MA5-23555) and anti-SCA-1 FITC 1:50 (BD Pharmingen 557405) ...
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bioRxiv - Genetics 2020Quote: ... A single gRNA and single strand HDR oligo were co-transfected along with the gRNA (1-2 μg of each plasmid) into 1-2 × 106 WT ESCs using Lipofectamine 2000 (Invitrogen #1168-019) in a single well of a 6 well plate ...
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bioRxiv - Bioengineering 2024Quote: ... and HITI insert were first mixed with a ratio of 2:2:1 with the reagent P3000 (ratio of DNA: P3000 of 1:2) in Opti-MEM™ Reduced Serum Medium (Gibco™), and then added to Opti-MEM™ Reduced Serum Medium (Gibco™ ...
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bioRxiv - Genomics 2023Quote: ... coated dishes in N2 medium (high-glucose DMEM, 1% N-2, 2% B-27 and 1% penicillin/streptomycin, from Thermo Fisher Scientific) at a density of 2×105 cells/cm2 ...
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bioRxiv - Cell Biology 2021Quote: Cross-linking experiments of Sgo11-415 and CPCISB10-280 were performed using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC, Thermo Fisher Scientific) in the presence of N-hydroxysulfosuccinimide (NHS ...
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bioRxiv - Microbiology 2019Quote: ... and then induced to differentiate by culturing for further 3 days in a 3:1 mixture of DMEM and Ham’s F12 media (Invitrogen, Palo Alto, CA) containing FCS (10%) ...
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bioRxiv - Cell Biology 2022Quote: ... The C2C12 cells were transfected with 2 μg plasmids (L-Cas9n-EGFP:R-Cas9n-puro=3:1) by electroporation (1650 v/10 ms/3 pulses) with the Neon Transfection System Kit (Thermo Fisher Scientific). The cells were seeded overnight to 24-well plates containing an antibiotic-free complete mediums ...
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bioRxiv - Developmental Biology 2022Quote: ... Maturation of released oocytes was induced by incubating for 1h at 16C in 3 μM 1-Methyladenine (Fisher Scientific, 5142-22-3). All embryos were raised in 0.22 μm filtered sea water (FSW ...
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bioRxiv - Immunology 2022Quote: ... targeting SAMHD1 (sense RNA 5’-GCAGAUAAGUGAACGAGAUTT-3’, antisense RNA 5’-AUCUCGUUCACUUAUCUGCAG-3’) or the negative control #1 siRNA using RNAiMAX (ThermoFisher, 13778-075). 24 h after transfection ...
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bioRxiv - Microbiology 2024Quote: ... 1 μg of plasmid and 3 μL of Lipofectamine 2000 (m/v = 1:3) were diluted in 0.1 mL of Opti-MEM (Gibco, 31985070, Gaithersburg, MD). After 5 min of incubation ...
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bioRxiv - Microbiology 2024Quote: ... was added at a 1:2000 dilutions for 1 h at 37 °C, followed by adding TMB (3, 3, 5, 5′-tetramethylbenzidine) peroxidase substrate (Thermo Fisher Scientific) for 30 min ...
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bioRxiv - Immunology 2023Quote: ... and activated with a mixture of 400 mM 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride and 100 mM N-hydroxysuccinimide (Thermo Fisher Scientific). The activated chip was lawned with goat anti-human IgG Fc (50 µg/mL ...
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bioRxiv - Immunology 2023Quote: ... and activated with a mixture of 400 mM 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride and 100 mM N-hydroxysuccinimide (Thermo Fisher Scientific). The activated chip was coupled directly to mAbs diluted to 10 µg/mL in 10 mM sodium acetate (pH 4.5 ...
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bioRxiv - Cell Biology 2023Quote: ... 5’-GAGACCCUAUCCGUGAUUAtt-3’ and antisense: 5’- UAAUCACGGAUAGGGUCUCtt-3’ (Silencer Select, rat negative control #1; scrambled siRNA and all siRNAs were from Ambion, Life Technologies). Transfection complexes were prepared in accordance with the instructions provided by the manufacturer and added to 2×105 cells seeded per well in 24-well plates.
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bioRxiv - Microbiology 2023Quote: ... The chip was activated with a freshly prepared solution of 130 mM 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) (Pierce PG82079) and 33 mM N-hydroxysulfosuccinimide (Sulfo-NHS) (ThermoFisher Scientific 24510) in 0.1 M MES pH 5.5 using the SFC ...
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bioRxiv - Microbiology 2023Quote: ... The chip was activated with a freshly prepared solution of 130 mM 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride (EDC) (Pierce PG82079) and 33 mM N-hydroxysulfosuccinimide (Sulfo-NHS) (ThermoFisher Scientific 24510) in 0.1 M MES pH 5.5 using the SFC ...
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bioRxiv - Bioengineering 2021Quote: ... 50 μg of pre-aliquoted Tandem Mass Tag 6-plex (TMT-6, Thermo Scientific) was resuspended in 20 μL anhydrous acetonitrile ...
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bioRxiv - Cancer Biology 2022Quote: ... Plasma IL-6 was measured by immunoassay (ProQuantum Human IL-6 Immunoassay Kit, ThermoFisher) and run on the ABI 7300 (Applied Biosystems ...
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bioRxiv - Bioengineering 2023Quote: FsC[Fe3+] was labelled by 6-carboxyfluorescein (6-FAM) (Thermo Fisher Scientific, Waltham, USA) to indicate the targeting of FsC[Fe3+] to C ...
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bioRxiv - Cell Biology 2020Quote: ... rabbit anti-Claudin-4 (1:200, Thermo Fisher Scientific, 36-4800), rabbit anti-Hopx (1:100 ...
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bioRxiv - Bioengineering 2019Quote: ... overnight at 4°C followed by AlexaFluor-568 (1:500, Invitrogen, A21099 and AlexaFluor-647 antibody (1:500 ...
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bioRxiv - Bioengineering 2021Quote: ... and ethidium homodimer-1 (4 µM, stains dead cell nuclei, Invitrogen) for later timepoints.
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bioRxiv - Neuroscience 2020Quote: Cells were loaded with 1 μM Fluo-4-AM (Thermo Fisher) in culture medium and incubated at 37 °C and 5% CO2 for 30 minutes ...
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bioRxiv - Neuroscience 2020Quote: ... were used at 4°C overnight: anti-GFP (1:250, Invitrogen), anti-DsRed(RFP ...
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bioRxiv - Microbiology 2020Quote: ... Mouse monoclonal anti-Claudin-4 (1:100, Novex, Life Technologies, USA) and rabbit polyclonal anti-claudin-4 (1:1000 ...
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bioRxiv - Neuroscience 2020Quote: ... a 1:4 dilution of trypsin was used (Gibco, Cat# 15400054) to split the cells into thirds.
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bioRxiv - Bioengineering 2022Quote: ... eBCs were stained with polyclonal Synaptotagmin-4 antibody (1:50; Invitrogen) and incubated at 4 °C overnight ...
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bioRxiv - Bioengineering 2020Quote: dLNs were fixed for 1 hour with 4% paraformaldehyde (Thermofisher Scientific), embedded in Tissue-tek OCT (VWR ...
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bioRxiv - Neuroscience 2021Quote: ... GPC-4 (#13048-1-AP, Thermo Scientific, and #MABN528, Sigma-Aldrich).
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bioRxiv - Cancer Biology 2020Quote: ... overnight at 4 °C and alpha-Tubulin (Invitrogen, 11224-1-AP) for 1-2 hours at room temperature ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Aliquots of 4 µL of 1 mM Mitotracker Green FM (ThermoFisher) stock solution were added to the samples and were kept in the dark at 15°C for 15–20 minutes ...
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bioRxiv - Cell Biology 2020Quote: ... 1:200 rabbit anti-FABP-4 (Thermo Fisher Scientific, PA5-30591), 1:200 rabbit anti-Osteocalcin (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2021Quote: ... the aliquots were incubated with 4-pentyn-1-o1 (ThermoFisher Scientific) and ADPRC (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2020Quote: ... KP-4 and PANC-1 cells were maintained in DMEM (Gibco). LOX-IMVI cells were maintained in RPMI 1640 medium (Gibco) ...
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bioRxiv - Cancer Biology 2022Quote: ... ki67 (ThermoFisher, Cat. RM-9106; 1:50 overnight at 4 °C), βIII-tubulin (Biolegend ...
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bioRxiv - Neuroscience 2024Quote: ... and mouse anti-HuC/D (Elavl3/4) (1:500, Invitrogen A21271) in PBST overnight at 4 °C in a humidified chamber ...
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bioRxiv - Microbiology 2024Quote: ... A 1:4 dilution of Intracellular Fixation Buffer (Invitrogen, Waltham, MA) was added to each chamber ...
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bioRxiv - Microbiology 2023Quote: ... plus DAPI (Thermo Fisher Scientific, 1:1000 in 4% BSA PBS) at room temperature ...