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Citations for Thermo Fisher :
3151 - 3200 of 10000+ citations for 6 TRIFLUOROMETHYL 1 2 3 4 TETRAHYDRO ISOQUINOLINE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... a sequencing reaction was performed with 2 pmol of unmodified RNA and 2 µl of a 2 mM AS primer 1 labeled with Ned (Life Technologies SAS, France). Reverse transcription was performed as for the SHAPE (+ ...
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bioRxiv - Cancer Biology 2024Quote: ... packaging plasmid psPAX2 and pMD2G in a ratio of 2:2:1 using lipofectamine 3000 (ThermoFisher, L3000001) and transduced into NES cells and ONS76 cells ...
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bioRxiv - Developmental Biology 2021Quote: ... cells were cultured for 4 days with 1 µM 4-OHT in Dulbecco’s Modified Eagle Medium (DMEM) supplemented with GlutaMax (Thermo Fisher Scientific), non-essential amino acids (NEAA ...
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bioRxiv - Cell Biology 2021Quote: ... and the pellets were dissolved in 0.1% SDS/ 0.67 M Tris-HCl pH 8 +/- 15 mM 4-acetamido-4’-maleimidylstilbene-2,2’-disulfonic acid (Thermo Fisher Scientific, USA). The samples were incubated at 37 °C for 2 h ...
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bioRxiv - Molecular Biology 2022Quote: ... the mix was cooled down to 4°C and supplemented with 1 μl of a solution containing 4 U Terminal Transferase (Thermo Scientific), 0.1 μl 10X NEB 4 buffer (NEB) ...
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bioRxiv - Cancer Biology 2021Quote: ... Drug dilution series (1:3) were prepared in PDAC organoid culture medium containing 1 μg/ml Hoechst (Invitrogen) and 1 μg/ml PI (Sigma) ...
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bioRxiv - Physiology 2022Quote: ... Plasmid DNA was mixed with PEI (1 mg/ml) at 1:3 in Opti-MEM medium (Gibco, 11058021) and added to cells after short incubation ...
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bioRxiv - Genetics 2022Quote: Calu-3 cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM)/F12 (1:1) GlutaMAX™ (ThermoFisher Scientific) supplemented with 10% FBS ...
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bioRxiv - Cancer Biology 2022Quote: ... were added to the tumoroids in a 1:3 and 1:10 effector:target (E:T) ratio in RPMI 1640 (Gibco) supplemented with 10% FBS ...
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bioRxiv - Biochemistry 2023Quote: ... cells were transfected with 3 μg mRNA at a mass ratio of 1:1 using Lipofectamine MessengerMAX (Invitrogen) according to the manufacturer’s guide ...
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bioRxiv - Neuroscience 2024Quote: ... stained with TO-PRO-3 diluted 1:1000 in DPBS and 1 µM Hoechst 33342 (Thermo Fisher 62249) nuclear counterstain for 30 minutes at room temperature ...
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bioRxiv - Microbiology 2019Quote: ... ATPase was assayed by a continuous spectrophotometric method using a 2-amino-6-mercapto-7-methylpurine ribonucleoside/purine nucleoside phosphorylase reaction to detect the released inorganic phosphate (EnzChek Kit; Life Technologies, California, USA) (53) ...
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bioRxiv - Cell Biology 2020Quote: 2 × 105 cells were transfected in a gelatin-coated 6-well plate in ES DMEM-2i using Lipofectamine 2000 (Fisher Scientific, 11668-027). Two pairs of gRNA were used for each gene (1 µg each ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were subsequently plated on islands of glial cells at a density of 1.25 × 104 cells/cm2 in 6-well plates in Neurobasal-A supplemented with 2% B27 and 0.2% penicillin/streptomycin (Invitrogen/ Thermo Fisher, Waltham, MA). Lentiviral particles encoding synaptoPAC were added 1–4 days after plating ...
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bioRxiv - Developmental Biology 2019Quote: The compound 5-(and-6)-carboxy-2’,7’-dichlorodihydrofluorescein diacetate (carboxy-H2DCFDA, Image-iT LIVE Green Reactive Oxygen Species Detection Kit, Molecular Probes; Invitrogen, I36007) was used to visualise the in vivo production of ROS ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Cells were washed then incubated in 8 μM of the ROS sensitive probe 5-(and-6)-chloromethyl-2′,7′-dichlorodihydrofluorescein diacetate acetyl ester (CM-H2DCFDA) (Molecular Probes, Eugene Oregon), for 45 minutes ...
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bioRxiv - Cancer Biology 2021Quote: ... Germany) and 2 drops of glacial acetic acid (#3738.1; Roth, Karlsruhe, Germany) in 70% EtOH (#200-678-6, Fisher Scientific, Waltham, Massachusetts, USA) for 30 s and subsequently rinsed in aqua bidest ...
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bioRxiv - Molecular Biology 2022Quote: ... HEK293 cells (wild-type or mS37 knock-out) were transfected in 6-well cell culture dish at 70% confluency with 2 μg plasmid using Lipofectamine 2000 (ThermoFisher scientific, Cat#11668027). Cells were collected after 72h ...
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bioRxiv - Neuroscience 2024Quote: Collected rosettes were plated onto a polyhema-treated 6-cm Petri dish in NDMB medium containing NDM and 2% B27 w/o vitamin A (Gibco, Grand Island, NY), supplemented with 10ng/ml IGF-1 ...
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bioRxiv - Immunology 2024Quote: ... Magnetic bead removal and the evaluation of CAR expression on T cells by flow cytometry were performed on day 6 by staining with a goat anti-mouse F(ab’)2 antibody (Invitrogen, Carlsbad, CA, USA). CART cells were harvested and cryopreserved on day 8 for future experiments ...
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bioRxiv - Neuroscience 2020Quote: ... serum-free medium (DMEM:F12 at 2:1) supplemented with N2 (1% v/v; Invitrogen), 1mM L-glutamine ...
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bioRxiv - Biochemistry 2024Quote: ... Solutions were further diluted to 1 µM using 2:1 MeOH:CHCl3 (ThermoFisher Scientific, Australia) with 5 mM ammonium acetate (SigmaAldrich ...
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bioRxiv - Neuroscience 2024Quote: ... supplemented with 2% B27 and 1% L-glutamine and 1% Pen-Strep (Life Technologies). Dissociated wildtype and Ncan KO cortical neurons were initially infected with Control and the two Ncan shRNA AAVs at 7 days in vitro (DIV ...
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bioRxiv - Neuroscience 2023Quote: ... supplemented with 2% B27 and 1% L-glutamine and 1% Pen-Strep (Life Technologies). Dissociated cortical neurons were infected with Control and Ncan shRNA AAVs at 7 days in vitro (DIV ...
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bioRxiv - Neuroscience 2023Quote: ... Alexa647 ICAM-2 at 1:100 and Live/Dead at 1:1000 (L34962, Invitrogen), for sorting of CD45neg ...
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bioRxiv - Biochemistry 2024Quote: ... 1 mM 2-mercaptoethanol and 1 mM PMSF) by sonication (using a Fisher Scientific Sonic Dismembrator with a Qsonica Ultrasonic Sonicator converter ...
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bioRxiv - Cancer Biology 2024Quote: ... at a 2:1:1 ratio using Lipofectamine 3000 transfection reagents (Invitrogen, Cat. L3000015). Viral supernatants were collected 48 hours after transfection and filtered through a 0.45-μm membrane (Corning ...
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ISL2 is an epigenetically silenced tumor suppressor and regulator of metabolism in pancreatic cancerbioRxiv - Molecular Biology 2020Quote: ... cells were stained by BODIPY™ FL C12 (4,4-Difluoro-5,7-Dimethyl-4-Bora-3a,4a-Diaza-s-Indacene-3-Dodecanoic Acid) (Thermo Fisher Scientific #D3922) to determine the amount of lipid droplet in regular conditions ...
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bioRxiv - Microbiology 2020Quote: ... 1.5×107 cells were incubated for 4 hours with 3 μL of AHA diluted in DMSO (Acros Organics via ThermoFisher Scientific, Germany) to final concentrations of 0 ...
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bioRxiv - Microbiology 2020Quote: ... 1.5×107 cells were incubated for 4 hours with 3 μL of AHA diluted in DMSO (Acros Organics via ThermoFisher Scientific, Germany) to final concentrations of 0 ...
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bioRxiv - Plant Biology 2019Quote: To assess GUS expression driven by the maize ubiquitin promoter in transgenic barley transformed with the pBRACT214m-GUS construct we collected different tissues and stained these with 1mg/ml of X-gluc (5-bromo-4-chloro-3-indolyl-B-D-glucuronic acid, Thermo Scientific, USA) in X-gluc buffer (100mM sodium phosphate buffer pH 7.0 ...
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bioRxiv - Immunology 2021Quote: ... Cells were then placed again into the Cytofunnel and spun at 800 RPM for 3 min using a Cytospin™ 4 Cytocentrifuge (Thermo Fisher). For intracellular visualization of IgM ...
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bioRxiv - Cell Biology 2021Quote: ... the catalysis of γ-glutamyl-3-carboxy-4-nitroanilide was measured at light absorbance at 410 nm using spectrophotometer (Thermo Fisher Scientific).
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bioRxiv - Neuroscience 2020Quote: ... and probed overnight at 4°C with the following primary antibodies diluted in 1x TBS-T with 3% BSA (Fisher Scientific, BP1600): α-CD11b (1:1000 ...
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bioRxiv - Microbiology 2020Quote: ... cells were washed 3 times in warm PBS and fixed for 45 min at room temperature in 4% (w/v) formaldehyde (Thermo Scientific; 28908) in PBS containing 0.1% Triton-X100 ...
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bioRxiv - Developmental Biology 2022Quote: ... Cells were passaged as aggregates every 3-4 days until they reach 60-70% confluency with 0.5mM EDTA diluted in PBS (Thermofisher, Canada. Catalog#15575020).
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bioRxiv - Cancer Biology 2022Quote: ... culture medium was replaced with 100 µL full melanoma medium with 4 µM Caspase-3/7 activity dye (CellEvent, Thermo Fisher Scientific). Plates were then imaged on a Celigo Imaging Cytometer (Nexcelom ...
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bioRxiv - Microbiology 2020Quote: ... The grids were blotted for 3-4 s and plunged into an ethane/propane mixture using a vitrification apparatus (Vitrobot, Thermo Fisher Scientific). For Fab 4G2-treated VLP EM sample preparation ...
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bioRxiv - Cancer Biology 2020Quote: ... Mice were immunized by injection of OVA coupled to the hapten 4-hydroxy-3-nitrophenylacetyl (NP-OVA) precipitated in alum (Imject® Alum, Thermo Scientific) into the hind footpads (25ul) ...
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bioRxiv - Cell Biology 2020Quote: ... Equal masses of lysates were separated by SDS-PAGE using either 4-12% Bis Tris gels or 3-8% Tris acetate gels (Thermo Fisher Scientific). All IRS2 immunoblots were separated on 3-8% Tris acetate gels with the exception of those shown in Figures 5A and S4B ...
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A Novel PHOX/CD38/MCOLN1/TFEB Axis Important For Macrophage Activation During Bacterial PhagocytosisbioRxiv - Immunology 2019Quote: ... cells were fixed with 4% paraformaldehyde (PFA) pH 7.4 at room temperature for 10 min and washed 3 times in PBS (Gibco Life Technologies,10010) for 5 min each ...
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bioRxiv - Genetics 2020Quote: ... 4,4-difluoro-5,7-dimethyl-4-bora-3a,4a-diaza-s-indacene 3-dodecanoic acid (BODIPY™ FL C12; Thermo Fisher Scientific, USA) (4 µg/mL ...
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bioRxiv - Neuroscience 2022Quote: ... and cells were passaged every 3-4 days once reaching 70-80% confluency using pre-warmed Versene (Fisher Scientific #15-040-066).
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McIdas localizes at centrioles and controls centriole numbers through PLK4-dependent phosphorylationbioRxiv - Molecular Biology 2022Quote: ... (3) 65%-95% for 5 min and (4) washout for 15 min) using the EASY-nano LC 1200 system (Thermo Fisher Scientific) with a flow rate of 300 nL/min ...
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bioRxiv - Developmental Biology 2022Quote: ... All other lines were passaged at approximately 80% confluency (every 4-5 days on average) as tiny clusters (3-5 cells on average) using 0.5 mM EDTA (15575020, Gibco, concentration 10 mM).
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bioRxiv - Developmental Biology 2022Quote: ... Epithelial sheets of embryos from the same litter were pooled (3-4 embryos per sample) and incubated for 10 min at RT in TrypLE Select Enzyme (Life Technologies/Gibco). Dissociated cells were resuspended in 0.5% BSA ...
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bioRxiv - Cell Biology 2022Quote: HTM cells cultured atop ECM hydrogels in presence of the different treatments for 3 days were fixed with 4% paraformaldehyde (Thermo Fisher Scientific) at room temperature for 20 min ...
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bioRxiv - Microbiology 2024Quote: ... Plates were washed five times with PBS and then twice with distilled water (dH2O) before addition of 5-bromo-4-chloro-3-indolyl-phosphate/NBT (BCIP/NBT) one-step solution (Thermo Fisher Scientific) and incubation at 37°C for approximately 15 minutes ...
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bioRxiv - Molecular Biology 2024Quote: ... We used siDESIGN Center (Horizon Discovery) to design siRNA (siUNG#4) targeting the 3′UTR region of UNG mRNA (Thermo Fisher Scientific).
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bioRxiv - Biochemistry 2024Quote: HEK-293 cells (ATCC, CRL-1573) were subcultured every 3-4 days in Dulbecco’s modified Eagle’s medium (Thermo Fisher Scientific, 41965-039) supplemented with 10% v/v fetal bovine serum (Sigma Aldrich ...