Labshake search
Citations for Thermo Fisher :
3001 - 3050 of 10000+ citations for 1 2 Dihydro 1 2 tetrahydro 2H pyran 2 yl oxy ethyl 5H tetrazole 5 thione d4 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Evolutionary Biology 2022Quote: ... A total of 2 x 106 cells (ratio 1:1) were resuspended in a final volume of 100 uL in DMEM (Invitrogen) and injected into the hypodermis of the mouse skin ...
-
bioRxiv - Microbiology 2022Quote: ... Mice were sacrificed at 1 dpi and lungs were collected in 1 mL DMEM/2% FBS/1X Antibiotic-Antimycotic (Gibco). Samples were homogenized and debris was clarified at maximum speed for 10 minutes at 4°C ...
-
bioRxiv - Microbiology 2023Quote: Sorted cells were cultured in 2 ml RPMI 5.5 mM glucose (1:1 mixture of Cat. No. 11875093 and Cat. No. 11879020 [Thermo-Fisher Scientific]) supplemented with 20% FCS (v/v) ...
-
bioRxiv - Neuroscience 2023Quote: ... Dissociated cells were suspended in culture medium (Neurobasal medium, 2% B27, 1% GlutaMAX™, GIBCO, 1% antibiotic/antimycotic (ThermoFisher Scientific)) supplemented with mouse NGF (50ng/μl ...
-
bioRxiv - Immunology 2024Quote: ... HeLa (CCL-2, ATCC), COS-1 (CRL-1650, ATCC) and L-929 (CCL-1, ATCC) cells were maintained in DMEM (11995073, Gibco) supplemented with 1% Penicillin-Streptomycin (15140122 ...
-
bioRxiv - Developmental Biology 2023Quote: ... The culture medium consisted of a 1:1 mixture of DMEM with 2 mM l-glutamine and F12 (Ham’s Nutrient Mix) (both from Life Technologies) and was supplemented with 10% (vol/vol ...
-
bioRxiv - Immunology 2023Quote: The whole blood samples were 1:1 diluted with 2% fetal bovine serum (FBS) (Hyclone, SH30406.05) in phosphate buffered saline (PBS) (Invitrogen, C10010500BT) and subjected to Ficoll (Cytiva ...
-
bioRxiv - Bioengineering 2023Quote: ... RPMI 1640 containing 2 μM calcein AM and 4 μM EthD-1 along with 1 mg/mL DAPI (ThermoFisher, 62248) was added to the 384-deep well plate (80 μL/well) ...
-
bioRxiv - Neuroscience 2023Quote: ... The base medium was gradually transitioned to N2/B27 medium (Neurobasal medium, 2% B27 supplement, 1% N2 supplement, 1% Glutamax, (ThermoFisher)) in 25% increments ...
-
bioRxiv - Genomics 2022Quote: ... resuspended in 1 mL NSB with 1:20 dilution of 0.25 mg/ml 4’,6-diamidino-2-phenylindole (DAPI, Invitrogen D1306) and FACS sorted ...
-
bioRxiv - Biophysics 2023Quote: ... the samples were added with a DNA-binding dye 4’,6-diamidino-2-phenylindole (DAPI, 1 μg mL-1 in PBS, Invitrogen) along with the secondary antibody solution ...
-
bioRxiv - Neuroscience 2023Quote: ... slices were incubated in PBS for 1 hour at room temperature and incubated 2 hours at room temperature with the appropriated Alexa488 (1/400, Invitrogen), Alexa594 (1/400 ...
-
bioRxiv - Neuroscience 2023Quote: ... and re-plated on Matrigel-coated dishes in NPC Medium (DMEM/F12, 2% B27, 1% N2, 1% Pen-strep; all from Gibco) supplemented with 20 ng/mL bFGF (Peprotech) ...
-
bioRxiv - Cancer Biology 2023Quote: ... Buffy coat was mixed 1:2 with PBS and added onto a Ficoll gradient in a 3:1 ratio (Invitrogen). This was centrifuged at 2100 rpm for 25 minutes at RT (w/o brakes) ...
-
Autoimmune inflammation triggers aberrant astrocytic calcium signaling to impair synaptic plasticitybioRxiv - Neuroscience 2023Quote: ... Sections were washed three times in TBS for 10 min each before incubation for 1-2 h at RT with the following Alexa Fluor secondary antibodies made in goat (1:500; Invitrogen): anti-rabbit Alexa 488 (A11008) ...
-
bioRxiv - Cell Biology 2023Quote: ... Counter-staining cell nuclei with a DNA-binding dye 4′,6-diamidino-2-phenylindole (DAPI, 1 μg ml−1 in PBS) and F-actin with Alexa Fluor dye conjugated phalloidin (Invitrogen) was also done at this step ...
-
bioRxiv - Neuroscience 2022Quote: ... the medium was changed to neural induction media (1:1 mixture of DMEM/F-12 GlutaMAX: Neurobasal media supplemented with 0.5x N-2 (Life Technologies), 0.5x B-27 (Life Technologies) ...
-
bioRxiv - Biophysics 2023Quote: ... the purified DNA in 1 ml NaHCO3 (pH 8.3) was incubated with 1 mM SM(PEG)2 (Thermo Scientific Pierce) for 20 mins at 20-22°C ...
-
bioRxiv - Cancer Biology 2023Quote: BE(2)-C (ATCC, CRL-2268) cells were cultured in a 1:1 mixture of DMEM/F12 (with HEPES, Gibco) and EMEM (ATCC) ...
-
bioRxiv - Cell Biology 2023Quote: ... were grown in high-glucose DMEM with supplements (1% penicillin/streptomycin, 10% FBS, 1% Non-essential Amino Acids [NEAA; Wako], 55 µM 2-Mercaptoethanol [Gibco]). Mouse embryonic stem (ES ...
-
bioRxiv - Neuroscience 2023Quote: ... Media was partially renewed every 3-4 days with neuronal differentiation media 2 (NDM2: 1:1 DMEM/F12:NB (Gibco), glutamax (Gibco) ...
-
bioRxiv - Microbiology 2023Quote: ... SARS-CoV-2 Nucleocapsid antibody (produced in mouse) was procured from (MA5-35943/Invitrogen/Massachusetts, USA/1:1000-1:10,000) used as primary antibody ...
-
bioRxiv - Microbiology 2023Quote: ... buffer (1× PBS containing 2% FBS) followed by incubation with Alexa Flour 633 goat anti-human IgG (1: 2000 dilution, Invitrogen). Finally ...
-
bioRxiv - Cell Biology 2024Quote: ... plasmid (EV or HLA-A2/Kb; 1 µg/µL) complex at 2:1 ratio prepared in Opti-MEM (Gibco, UK) for 18 hours in the presence or absence of 100 ng/mL type I IFNα (PBL Assay Science ...
-
bioRxiv - Neuroscience 2024Quote: ... Cell cultures underwent half-media changes with serum-free media (Neurobasal Medium, 2% B27 supplement, 1% 200 mM L-glutamine, and 1% penicillin/streptomycin; all Gibco) two hours post-plating and maintained every 3-4 days for a total culturing period of 21 days ...
-
bioRxiv - Neuroscience 2024Quote: ... and cells were resuspended in plating media (Neurobasal medium, 2% B27 supplement, 1% 200 mM L-glutamine, 1% penicillin/streptomycin (Gibco), 5% Fetal bovine serum (Cytiva ...
-
bioRxiv - Cell Biology 2024Quote: ... 1 μg total RNA was mixed with 2 μl 1:100 diluted ERCC RNA Spike-In Mix (Thermo Fisher Scientific). Libraries were prepared with the NEBNext Ultra II Directional RNA Kit (NEB) ...
-
bioRxiv - Microbiology 2024Quote: ... We treated them with a 1:1 mixture of conditioned medium and normal culture medium (DMEM with high glucose [Sigma], 2% fetal bovine serum [ThermoFisher Scientific] ...
-
bioRxiv - Microbiology 2024Quote: ... the PBS or virus dilution in PBS was aspirated and 3 ml of overlay consisting of 1:1 2 x DMEM (DMEM high glucose, no sodium bicarbonate buffer powder [Gibco # 12-100-046] in 500 mL of RNase-free water ...
-
bioRxiv - Synthetic Biology 2024Quote: ... DNA labeling was performed using PBS containing 1 µg/ml 4′,6-diamidino-2-phenylindole (DAPI) (1:10000) (Invitrogen, D1306). After 3 additional washes with PBS ...
-
bioRxiv - Microbiology 2024Quote: ... 1% w/v BSA) were precomplexed with anti-human Fc specific antibodies-HRP (A18817, ThermoFisher Scientific, 2:1 ratio lectin:antibody) and pre-incubated with 20 mM EDTA or 40 mM glucose ...
-
bioRxiv - Cell Biology 2024Quote: ... the cells were dissociated to single cells with Accutase supplemented with 10 µM Y-27632 and replated in neural maintenance medium (Neurobasal Plus, supplemented with 2% B27+ supplement, 1% Pen/Strep, 1% GlutaMax (all ThermoFisher) supplemented with 10 ng/mL BDNF and 5 ng/mL GDNF (both R&D Systems) ...
-
bioRxiv - Biochemistry 2024Quote: ... pH 7.4, 10 mM KCl, 2 mM MgCl2, 1 mM EDTA, 1 mM EGTA with protease and phosphatase inhibitors Thermo Scientific, Waltham ...
-
bioRxiv - Biophysics 2021Quote: ... we labeled 5-30 μM protein with 2 mM DTNB (Ellman’s reagent, Thermo Scientific) in 20 mM Tris ...
-
bioRxiv - Cancer Biology 2021Quote: ... RNA (2-5 µg) was treated with DNAse I (Thermo Fisher Scientific, Cat # AM1906) according to the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2020Quote: ... HEK293T cells were incubated with 10 µM 5-ethynyl-2’ deoxyuridine (EdU, Thermo Fisher) for 20 min ...
-
bioRxiv - Cell Biology 2020Quote: ... Slides were then blocked with 2% BSA and 5% donkey serum (ThermoFisher Cat # NC9624464) in PBS for 1 hour ...
-
bioRxiv - Developmental Biology 2021Quote: Click-iT EdU (5-ethynyl-2’-deoxyuridine, a thymidine analog) kit from Life Technologies was used to perform DNA replication assay (Milton et al. ...
-
bioRxiv - Genomics 2020Quote: ... 5 µl of 10X Turbo DNase buffer and 2 µl Turbo DNase (Thermo Fisher Scientific ...
-
bioRxiv - Systems Biology 2020Quote: ... The samples were then reduced with 5 mM Tris(2-carboxyethyl)phosphine (ThermoFisher Scientific) for 30 minutes at 37 °C and then alkylated in the dark for 30 minutes at 25 °C with 40 mM iodoacetamide (Sigma Aldrich) ...
-
bioRxiv - Cancer Biology 2020Quote: ... and 5 μl of purified products were loaded on 2% E-Gel EX (Invitrogen) to verify the reaction performance and amplicon size ...
-
bioRxiv - Neuroscience 2021Quote: ... and two injections of 5-ethynyl-2’-deoxyuridine (EdU: 0.01 mg/kg, Invitrogen #A10044), made as a 10 mM stock solution in DMSO and diluted for injection to 40% strength in 0.9% sterile saline ...
-
bioRxiv - Molecular Biology 2022Quote: ... mixed with 5 μl of 2 x Novex TBE-Urea sample buffer (ThermoFisher Scientific) and denatured for 3 minutes at 70°C ...
-
bioRxiv - Molecular Biology 2022Quote: ... For labeling of proliferating cell populations with 5-ethynyl-2’-deoxyuridine (EdU) (ThermoFisher Scientific), animals received IP injections of EdU (50 mg/kg ...
-
bioRxiv - Biophysics 2022Quote: ... 5 μM Fluo-2 Low Affinity (TefLabs) and 2.5 μM Alexa568 (Invitrogen, Waltham MA) as previously described (36) ...
-
bioRxiv - Neuroscience 2022Quote: ... passed through Xylol (2 x 5 minutes) and immediately mounted with Eukitt® (ThermoFisher).
-
bioRxiv - Developmental Biology 2020Quote: ... viride were incubated in 100 μM 5-ethynyl-2’-deoxyuridine (EdU; Thermo Fisher Scientific) diluted in ASW from 24 to 48 HPA ...
-
bioRxiv - Microbiology 2021Quote: ... All medium were supplemented with 2-5 or 10% fetal bovine serum (FBS; Gibco) according to the experimental setting ...
-
bioRxiv - Neuroscience 2021Quote: ... and 2-(4-Iodophenyl)-3-(4-nitrophenyl)-5-phenyltetrazolium Chloride (INT, #I00671G, Fisher Scientific).
-
bioRxiv - Neuroscience 2020Quote: ... a ClickiT ® EdU (5-ethynl-2’-deoxyuridine) cell proliferation assay (Invitrogen, Paisley, UK) was used ...