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Citations for Thermo Fisher :
2951 - 3000 of 10000+ citations for 1 2 Dihydro 1 2 tetrahydro 2H pyran 2 yl oxy ethyl 5H tetrazole 5 thione d4 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... anti-claudin-2 (21H12) (Cat. No. 32-5600, 1:1000, Thermo Scientific, Waltham, MA, USA), anti-E-Cadherin (Cat ...
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bioRxiv - Molecular Biology 2024Quote: ... Secondary antibody anti-rabbit Alexa Fluor 488 F(ab’)2 (A11070, Invitrogen, dilution 1:500) in PBS-Tx supplemented with 1% BSA was incubated overnight at room temperature ...
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bioRxiv - Molecular Biology 2024Quote: ... and 4’,6-diamidino-2-phenylindole (DAPI) for nuclear staining (H3570; Invitrogen; dilution 1:200), diluted in phosphate-buffered saline (PBS) ...
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bioRxiv - Cell Biology 2024Quote: ... for 1 hour and 2 μg/mL Hoechst 33342 (H1399, Thermo Fisher Scientific, MA, USA) for 15 minutes ...
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bioRxiv - Cell Biology 2024Quote: ... 2 μL of 20 μM fibrillarin siRNA (Supplementary Table 1) or control siRNA (ThermoFisher, 4390843) diluted in 250 μL Opti-MEM was combined with 6 μL Lipofectamine RNAiMAX diluted in 250 μL Opti-MEM ...
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bioRxiv - Biophysics 2024Quote: ... to visualize actin filaments and with 4’-6-diamidino-2-phenylindole (DAPI, Invitrogen, 1:200) for the nucleus ...
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bioRxiv - Developmental Biology 2024Quote: ... H3: dilute at 1:150 (Activemotif, 39763) along with 2 mM EDTA (Invitrogen, 15575- 038). Samples were incubated overnight at 4 °C on a nutator ...
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bioRxiv - Genomics 2024Quote: 1-2 μg of total RNA per sample were treated with DNase I (Invitrogen; AM2224), of which 200-500 ng were used for RNA-seq ...
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bioRxiv - Microbiology 2024Quote: ... and stained with 2-(4amidinophenyl)-1H-indole-6-carboxamidine (DAPI, 1 μg/mL; Invitrogen, D1306) and washed with Dulbecco’s PBS ...
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bioRxiv - Cell Biology 2024Quote: Lamellae were prepared on an Aquilos 1 or Aquilos 2 cryo-FIB-SEM (Thermo Scientific) as described for the Drosophila samples ...
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bioRxiv - Cancer Biology 2024Quote: ... MIA PaCa-2 cells received an additional supplementation of 1% horse serum (Gibco, #16050-122) in DMEM along with 10% FBS and 1% penicillin/streptomycin ...
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bioRxiv - Developmental Biology 2024Quote: ... and finally coated with 1-2 μg/ml laminin in Neurobasal media (NB media, Gibco) with 2% PSF at 37°C for 4 hours.
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bioRxiv - Microbiology 2024Quote: Luria-Bertani (LB) broth and agar (tryptone 10 g/L, yeast extract 5 g/L, sodium chloride 10 g/L, Fisher Scientific BP1426-2 and BP1425-2) were routinely used for growth of E ...
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bioRxiv - Immunology 2021Quote: The IL-2 ELISA was performed using the IL-2 Human ELISA Kit by ThermoFisher scientific according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2021Quote: ... pCG1-SARS-2-SdC19 or pCG1-SARS-2-SdC19-H2 using Lipofectamine 2000 (ThermoFisher Scientific) according to the manufacturer’s instruction ...
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bioRxiv - Immunology 2022Quote: ... 2-mercaptoethanol (RP-10) and 10 ng/ml recombinant human IL-2 (Thermo Fisher Scientific) for 18-22 hours ...
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bioRxiv - Biochemistry 2022Quote: ... Cells (70–80% confluent) were loaded with 2 μM Fura 2 AM (Molecular Probes, Oregon) in culture medium for 20 min at 37 °C in a humidified atmosphere of 95% O2 and 5% CO2 ...
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bioRxiv - Developmental Biology 2021Quote: ... and 0.1% 2-Mercaptoethnol (2-ME; cat. num. 21985023; Gibco, Thermo Fisher Scientific, Waltham, MA) supplemented with 0.1% L-ascorbic acid (ascorbate ...
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bioRxiv - Developmental Biology 2021Quote: ... and 0.1% 2-Mercaptoethnol (2-ME; cat. num. 21985023; Gibco, Thermo Fisher Scientific, Waltham, MA) supplemented with 0.1% L-ascorbic acid (ascorbate ...
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bioRxiv - Microbiology 2022Quote: ... HEK293T cells were transfected with 2 µg pcDNA3.1 CoV-2 Spike using Lipofectamine2000 (Thermo Fisher). At 24 hours post-transfection ...
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MK2 deficiency decreases mortality during the inflammatory phase after myocardial infarction in micebioRxiv - Physiology 2023Quote: ... supplemented with 10% FBS, 2% antibiotics (streptomycin and penicillin, Hyclone) and 2% amphotericin B (Gibco), plated onto two 35 mm petri dishes ...
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bioRxiv - Bioengineering 2023Quote: ... All samples were then incubated with 4′,6-Diamidino-2-phenylindole (DAPI, 2 µM, Thermofisher) as a nuclear counterstain and Alexafluor 555 phalloidin (1:60 ...
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bioRxiv - Immunology 2024Quote: ... and resuspended in PBS buffer without Ca+2 and Mg+2 (Gibco, Waltham, MA, USA).
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bioRxiv - Genetics 2023Quote: ... neuronal rosetes were passaged as small clusters in either a 1:1 or 1:2 ratio using the StemPro™ EZPassage™ Disposable Stem Cell Passaging Tool (Gibco, #23181010) or via hand-picking ...
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bioRxiv - Bioengineering 2023Quote: ... then incubated at room temperature in the dark for 2 h with secondary antibodies (AlexaFluor 488 goat anti-mouse IgG 1:400 or 1:600) or rhodamine phalloidin (Invitrogen, R415, 1:600) to visualize F-actin ...
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bioRxiv - Immunology 2020Quote: ... and 1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide (Thermo Fisher Scientific). The activated beads were washed three times with 50 mM MES pH 5.0 and added to SARS-CoV-2 S protein which was diluted in 50 mM MES pH 5.0 ...
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bioRxiv - Immunology 2022Quote: ... and 1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide (Thermo Fisher Scientific) and incubated for 30 min on a rotator at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... the sections were sequentially incubated with mouse polyclonal antibody to SARS-CoV-2 N protein (1:500 dilution) and HRP-conjugated goat anti-mouse IgG secondary antibody (1:5000 dilution, Invitrogen). The sections were observed under microscope (Olympus ...
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bioRxiv - Synthetic Biology 2021Quote: ... Regulatory T cells were activated at 1 x 106 cells mL-1 for 2 days in complete XVivo15 supplemented with magnetic Treg Xpander CTS Dynabeads (ThermoFisher) at a 1:1 bead to cell ratio and 500 U mL-1 of IL-2 (UCSF Pharmacy) ...
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bioRxiv - Cancer Biology 2020Quote: ... cells from the previous steps were barcoded by cotransfecting PB-EF1α-Puro-V8.2 library and Super PiggyBac Transposase plasmid (SBI #PB210PA-1) at a 1:10 molar ratio using Lipofectamine 3000 (Thermofisher). Barcoded cells were puromycin-selected for 7 d ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were then incubated for 1 hr with goat anti-rabbit Alexa Fluor 488F(ab’)2 Fragment (1:1000, A11070; Invitrogen) and Alexa Fluor 568 Phallodin (A12380 ...
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bioRxiv - Microbiology 2021Quote: ... the sections were incubated with house-made mouse anti-SARS-CoV-2 nucleocapsid protein serum (1:5000) and HRP465 conjugated goat anti-mouse IgG secondary antibody (1:5000 dilution, Invitrogen). The lung sections from the vector-transduced mouse were used as negative control ...
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bioRxiv - Microbiology 2021Quote: ... in 10% FBS for 1-2 hours followed by incubation with secondary goat anti-rabbit AlexaFluor 594 antibody (1:1000, Invitrogen) in 10% FBS for 30 min ...
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bioRxiv - Neuroscience 2021Quote: ... Medium was replaced at day 2 with N2B27 medium consisting of 1:1 pre-mixed neurobasal medium (#21103-049) and DMEM/F12 (#21331-020, Gibco), 1% B27 supplement without vitamin A (#12587-010 ...
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bioRxiv - Cancer Biology 2021Quote: 2–2.5×106 cells resuspended in a mix of 1:1 PBS and Geltrex Basement Membrane Mix (Thermo Fisher Scientific) were subcutaneously injected in the right flank of 10–12 weeks old NOD/Scid/gamma (NSG ...
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bioRxiv - Neuroscience 2020Quote: ... After 5 days the spheroids were transferred to Neural differentiation medium (NdM) (Neurobasal, 2% B27, 1% GlutaMAX, 1% P/S (all from Gibco) supplemented with 20 ng/mL of EGF and FGF2 (both PrepoTech ...
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bioRxiv - Bioengineering 2022Quote: ... with 50 µL per injection site of vaccine formulations containing 5 µg of SARS-CoV-2 antigen (RBD-SD1, S-2P, S-PCNA1, or 6RBD-np) gently mixed 1:1 (v/v) with AddaVax (Invitrogen) adjuvant ...
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bioRxiv - Cancer Biology 2022Quote: ... using base medium (1:1 ratio of DMEM/F12 Glutamax/Neurobasal medium [Gibco], 2% B27 supplement without vitamin A [Gibco] ...
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bioRxiv - Developmental Biology 2022Quote: ... WIK 24hpf samples were spiked with 2 μl of 1:100 diluted ERCC spike-in mix 1 (4456740, Thermofisher Scientific) while all other samples were spiked with 2 μl of 1:100 diluted ERCC spike-in mix 2 (4456740) ...
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bioRxiv - Neuroscience 2020Quote: ... and equally distributed in wells containing growth medium (DMEM-F12-glutamax [Gibco], 40 μg/mL insulin [Sigma], 1/200 B-27 supplement [Gibco], 1/100 N-2 supplement [Gibco] ...
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bioRxiv - Neuroscience 2021Quote: ... followed by 2-hour incubation with secondary antibody (1:200 Alexa 555 anti-rabbit [Invitrogen]; 1:200 Alexa 488 anti-chicken [Invitrogen]). Following three additional 10-min washes in PBST ...
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bioRxiv - Plant Biology 2022Quote: ... A second round of reverse transcription was initiated with the addition of 4 µL of reverse transcription mix (4:2:1:1 of First Strand Buffer, 0.1 M DTT, RNaseOUT, SuperScript II; ThermoFisher Scientific) to each sample then incubated at 25□ for 10 min ...
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bioRxiv - Cell Biology 2021Quote: ... the media was replaced entirely with fresh retinal differentiation media (RDM) (DMEM:F12 3:1, 2% B27 supplement, MEM NEAA, 1× antibiotic, antimycotic (Thermo Fisher) and 1× GlutaMAX)) ...
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bioRxiv - Neuroscience 2021Quote: ... 6-diamino-2-phenylindole (DAPI, 1:25,000) for 1 min and the sections were mounted in ProLong® Gold antifade reagent (Invitrogen).
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bioRxiv - Cell Biology 2020Quote: ... the media was changed to retinal differentiation medium (RDM;DMEM:F12 3:1, 2% B27 supplement, MEM NEAA, 1× antibiotic, antimycotic (Thermo Fisher) and 1× GlutaMAX) ...
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bioRxiv - Immunology 2021Quote: ... cells were alternatively pre-activated with IL-2 as above and one of: 1:1 anti-CD3/anti-CD28-coated beads (Gibco Dynabeads Human T-Expander or Human Treg Expander) ...
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bioRxiv - Microbiology 2021Quote: ... then covered with 2 mL overlay medium of 2% Avicel CL-611 (DuPont Pharma Solutions) diluted 1:1 with 2x minimum essential media (Gibco). The cells were then incubated for three days ...
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bioRxiv - Cell Biology 2021Quote: ... was added for 2 days (Day 1 and Day 2 of differentiation) followed by a neural induction period in Neural Induction Media (Advanced DMEM/F12 (1:1, Gibco), 1% non-essential amino acids (Gibco ...
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bioRxiv - Cell Biology 2021Quote: ... Beads were captured on the magnet and the supernatant now containing the dissociated cells was transferred to a 15 ml Falcon tube containing 10 ml ice cold FACS buffer (DMEM w/o phenol red + 1% P/S, 2% FBS, 1 mM EDTA pH 8, all from Gibco) and centrifuged for 5 minutes at 300 g and 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... the samples were added with a DNA-binding dye 4′,6-diamidino-2-phenylindole (DAPI, 1 µg mL-1 in PBS, Invitrogen) and phalloidin conjugated Alexa Fluor dye (Invitrogen ...