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Citations for Thermo Fisher :
2851 - 2900 of 6840 citations for H D Glu amc oh since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... goat anti-mouse IgG (H+L) antibody conjugated with Alexa Fluor 488 (A-11001; Thermo Fisher Scientific) (1,000× dilution) ...
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bioRxiv - Systems Biology 2022Quote: ... The samples were centrifuged at 100,000×g for 1 h at 4°C (S55A2 rotor, Thermo fisher). The pellets were resuspended in SDS sample buffer and subjected to western blot analysis.
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bioRxiv - Cell Biology 2020Quote: ... maintained in short-term (<24 h) tissue culture in RPMI 1640 (11879-020, Gibco, Thermo Fisher Scientific) containing 1% penicillin/streptomycin (1214-122 ...
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bioRxiv - Cell Biology 2020Quote: ... maintained in short-term (<24 h) tissue culture in RPMI 1640 (11879-020, Gibco, Thermo Fisher Scientific) containing 1% penicillin/streptomycin (1214-122 ...
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bioRxiv - Molecular Biology 2020Quote: ... One μg of DNase-free RNA was reverse transcribed using Maxima H Minus Reverse Transcriptase (Thermo Scientific). cDNA was then amplified in RT-qPCRs using LightCycler 480 SYBR Green I Master (Roche ...
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bioRxiv - Cell Biology 2020Quote: Cell viability of HCT8 cells treated with MakA for 48 h was quantified by PrestoBlue (Thermo Fisher) or MTS (Promega ...
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bioRxiv - Pathology 2020Quote: ... and Donkey anti-Rabbit IgG (H+L) ReadyProbes™ Secondary Antibody conjugated to Alexa Fluor 594 (ThermoFisher, Cat ...
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bioRxiv - Molecular Biology 2021Quote: ... for 1 h in an environment at room temperature and used ECL reagent (Thermo Fisher Scientific, Inc.) for development ...
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bioRxiv - Microbiology 2020Quote: ... Two µg of RNA was used to synthesize cDNA using Maxima H minus reverse transcriptase (ThermoFisher Scientific) and 1 µM of random hexamers (Promega ...
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bioRxiv - Microbiology 2020Quote: ... cDNA was synthesized using RevertAid H Minus First Strand cDNA synthesis kit (Thermo Fisher Scientific, Waltham, MA) according to the manufacturer’s instructions for highly structured mRNAs using oligo (dT)18 primers and 1μg of total RNA as template in a 20 μl reaction.
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bioRxiv - Microbiology 2021Quote: ... The aquatic fraction was dialyzed for 48 h with Slide-A-Lyzer dialysis cassettes by Thermo Fisher, with a 3,500 molecular weight cut-off ...
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bioRxiv - Biophysics 2021Quote: ... Samples were centrifuged for 3 h at 200,000 g (TST 60.4 swing-out rotor, Thermo Fisher/Sorvall). The gradients were manually fractionated ...
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bioRxiv - Cell Biology 2020Quote: ... The viability assay was performed 72 h later using the Live/Dead Kit (Thermo Fisher Scientific, L3224).
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bioRxiv - Plant Biology 2021Quote: ... First strand synthesis was accomplished using the RevertAid H Minus First Strand cDNA Synthesis Kit (Thermo Scientific) following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2020Quote: ... the cells were incubated for 1 h with secondary antibodies (Alexa Fluor 488, 568, 647-conjugated; ThermoFisher). After three PBS washes ...
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bioRxiv - Microbiology 2020Quote: ... then stained with Alexa Fluor 488-conjugated goat anti-rabbit IgG H+L antibody (Thermo Fisher Scientific).
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bioRxiv - Developmental Biology 2021Quote: Full-length cDNA synthesis from RNA was performed using Maxima H Minus reverse transcriptase (K1651, Thermo Scientific, Fisher Scientific ...
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bioRxiv - Immunology 2021Quote: ... cells were stained with Goat anti-Rabbit IgG (H+L) AF647 (Invitrogen, Cat. A21245 Lot. 223 2862) at a dilution of 1:1000 and 2 ug/ml Hoechst 33342 in 2% milk for 1 hour ...
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bioRxiv - Neuroscience 2020Quote: ... Autophagy was activated by keeping cells in HBSS for 2 h prior to treatment (24020-091, Invitrogen) and blocked by addition of 3-MA (Sigma-Aldrich ...
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bioRxiv - Genomics 2022Quote: ... Secondary antibodies were goat anti-rabbit IgG (H+L) Alexa Fluor 680 (1:10000; A-21109, ThermoFisher), goat anti-mouse IgG ...
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bioRxiv - Pathology 2021Quote: ... Secondary antibodies were goat anti-mouse IgG (H+L) AlexaFluor-488 (1:500, A-11001, ThermoFisher Scientific) and goat anti-rabbit IgG (H+L ...
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bioRxiv - Molecular Biology 2021Quote: ... and horse-radish peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1:5000 (Thermo Scientific, 31460). Secondary antibodies were visualized with ECL plus substrate (Pierce ...
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bioRxiv - Developmental Biology 2020Quote: ... cDNA synthesis was performed using the RevertAid H Minus First Strand cDNA Synthesis Kit (Thermo Fisher Scientific). Next ...
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bioRxiv - Cell Biology 2020Quote: ... all cDNA constructs were transfected (0.5 to 1μg) for 24 h using Lipofectamine 2000 (Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2020Quote: ... Cells were then incubated with anti-mouse IgG (H+L)-Alexa Fluor 660 secondary antibody (ThermoFisher Scientific) for 1 hour ...
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bioRxiv - Immunology 2020Quote: ... 24 h later supernatants were analysed for LDH activity using the Pierce LDH Cytotoxicity Assay Kit (ThermoFisher) and lysate protein content was evaluated by immunoblotting ...
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bioRxiv - Immunology 2021Quote: ... and Alexa Fluor 405 conjugated Goat anti-Mouse IgG (H+L) cross-absorbed secondary antibody (ThermoFisher Scientific). All isotype controls of fluorescence conjugated primary antibodies are from BioLegend ...
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bioRxiv - Immunology 2020Quote: ... 24 h later supernatants were analysed for LDH activity using the Pierce LDH Cytotoxicity Assay Kit (ThermoFisher) and lysates were evaluated by immunoblotting for caspase-1 (Cell Signaling Technology ...
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bioRxiv - Developmental Biology 2021Quote: ... and then incubated with Alexa Fluor 488-conjugated Goat Anti-mouse IgG(H+L) (Invitrogen catalogue #A32723) diluted 1:400 in 1% BSA/PBT for one hour at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... Supernatants were then mixed for 1 h with Protein A magnetic beads (Thermo Fisher Scientific, MA, USA), and lysates were then incubated overnight with primary anti-LRRC23 or Rabbit IgG (#2729 ...
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bioRxiv - Cell Biology 2021Quote: ... goat anti-rabbit IgG (H+L) conjugated to Alexa fluor 594 (Molecular Probes A-11012, 1:400). Purified Nb48::HA::6His raised against TbBILBO1 was used as a probe (10 μg/mL ...
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bioRxiv - Genomics 2021Quote: RNA was reverse-transcribed using the RevertAid H minus First Strand cDNA Synthesis kit (Invitrogen, CA, USA) with random hexamer primers following the manufacturer’s instructions.
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bioRxiv - Genomics 2020Quote: ... Used RNA template was removed by incubating with 0.5ul RNase H(5U/μl) and 0.5μl RNase cocktail (Ambion) at 37°C for 30min ...
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bioRxiv - Neuroscience 2022Quote: ... Alexa Fluor 405-conjugated goat polyclonal anti-rabbit IgG (H+L) (1:500; A31556, Thermo Fisher Scientific); Alexa Fluor 546-conjugated goat polyclonal anti-mouse IgG (H+L ...
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bioRxiv - Neuroscience 2022Quote: ... Alexa Fluor 546-conjugated goat polyclonal anti-rat IgG (H+L) (1:500; A-11081, Molecular Probes); Alexa Fluor 555-conjugated goat polyclonal anti-mouse IgG (H+L ...
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bioRxiv - Biochemistry 2022Quote: ... for 1 h at 30 °C and then stained with Alexa 647 goat anti-mouse IgM (Invitrogen) for 30 min at RT ...
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bioRxiv - Cell Biology 2022Quote: ... the peak corresponding to the [M+H]+ ion at 5ppm was integrated in Tracefinder 4.1 (Thermo Scientific). The enrichment of isotopically labelled glucose (13C6 glucose ...
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bioRxiv - Immunology 2022Quote: ... followed by 1 h of incubation with fluorescent dye-labeled secondary antibodies (Thermo Scientific, A11005 or A11008). After mounting with Cytoseal 60 (Thermo Scientific ...
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bioRxiv - Immunology 2022Quote: ... followed by 1 h of incubation with fluorescent dye-labeled secondary antibodies (Thermo Scientific, A11005 or A11007). After mounting with ProLong Gold antifade reagent with DAPI (Thermo Scientific ...
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bioRxiv - Neuroscience 2022Quote: ... washed and incubated for 2 h with Alexa Fluor 594-conjugated secondary antibody (1:1,000; Life Technologies). After a final washing step ...
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bioRxiv - Microbiology 2022Quote: PBMCs were thawed according to standard procedure and rested 2-3 h in IMDM (Gibco/ThermoFisher Scientific) containing 5% human AB serum (SAB ...
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bioRxiv - Microbiology 2022Quote: PBMCs were thawed according to standard procedure and rested 2-3 h in IMDM (Gibco/ThermoFisher Scientific) containing 5% human AB serum (SAB ...
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bioRxiv - Microbiology 2022Quote: ... Parasite growth was monitored every 24 h over four days following SYBR green I (Invitrogen, CA, USA) staining ...
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bioRxiv - Pathology 2022Quote: ... sections were incubated with biotin-labeled goat anti-rabbit IgG (H+L) (1: 500, #31460, ThermoFisher, USA) for 30 min at 37 °C ...
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bioRxiv - Plant Biology 2022Quote: Poly(dT) cDNA was prepared from 3 μg total RNA with RevertAid H Minus Reverse Transcriptase (ThermoFisher) and analyzed on StepOnePlus Real-Time PCR System (LifeTechnologies ...
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bioRxiv - Neuroscience 2022Quote: ... After 4-5 h bacteria were collected by centrifugation (5000 rpm, SLA 3000 rotor, ThermoFisher Cat#07149) for 15 minutes and resuspended by gentle trituration in ice-cold 50 ml of TBS Buffer (150 mM NaCl ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... elegans RNA in a thermal cycler using RevertAid H minus First Strand cDNA synthesis Kit (Thermo Scientific) according to manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... Secondary goat antibodies against mouse IgG and IgM (H+L) conjugated with Alexa 488 (#A-10680, Invitrogen) were diluted at 1:500 in PBS1X and incubated at 37°C for 30 min.
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bioRxiv - Plant Biology 2023Quote: ... Both the FHS-BBE23 and H-BBE23 codon-optimized fusion genes were synthesized by GeneArt (Life Technologies) and cloned in pPICZαB expression vector (Invitrogen ...
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bioRxiv - Neuroscience 2022Quote: ... Samples were subsequently incubated for ∼30 h with polyclonal anti-GFAP antibody (Thermofisher, catalog no. PA1-10019), monoclonal anti-MBP (BioLegend ...