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Citations for Thermo Fisher :
2951 - 3000 of 10000+ citations for 6 Chloropyrazolo 1 5 a pyridine 2 carboxylic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... 50 IU penicillin/streptomycin mL−1 and non-essential amino acids (Life Technologies, UK). Antibodies specific for HIF-1α were purchased from BD Biosciences (610959) ...
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bioRxiv - Immunology 2021Quote: ... 1x MEM non-essential amino acids solution and 1 mM sodium pyruvate (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2021Quote: ... 50μM B-Mercaptoethanol and 1 × MEM non-essential amino acids (all Gibco, Thermo-Fisher) and 20ng/ml GM-CSF (Biolegend ...
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bioRxiv - Plant Biology 2022Quote: ... supplemented with 10% fetal bovine serum and 1% MEM non-essential amino acids (Gibco) at 37 °C ...
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bioRxiv - Cell Biology 2024Quote: ... dried under vacuum and resuspended to 50 μl with 1% Formic Acid (ThermoFisher, #85178) and quantified using the Pierce Quantitative Fluorometric Peptide Assay (ThermoFisher ...
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bioRxiv - Physiology 2024Quote: ... in 1% aqueous acetic acid and imaged in an iBright CL1500 imaging system (Invitrogen). Membranes were then washed 3 times in Tris-buffered saline containing 0.1% Tween-20 (TBST ...
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bioRxiv - Biochemistry 2023Quote: All reactions were quenched by adding 1 μL of formic acid (Thermo Scientific, 85178) and diluted to a volume of 200 μL prior to desalting ...
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bioRxiv - Cell Biology 2023Quote: Cultured cells were treated with all-trans retinoic acid (RA, 1 µM, Fisher Scientific), high affinity pan-RAR antagonist AGN-193109 (AGN ...
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bioRxiv - Systems Biology 2023Quote: ... 0.1 mM non-essential amino acids and 1 mM sodium pyruvate (all Gibco, USA). The cells were grown in a humidified atmosphere with 5% CO2 at 37°C.
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bioRxiv - Cell Biology 2023Quote: ... Acid-solubilized bovine collagen 1 (Devro) was salt balanced using 10x DMEM (ThermoFisher Scientific) and pH neutralised using 0.1 M NaOH before combining with Matrigel and then the cell suspension on ice ...
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bioRxiv - Biophysics 2022Quote: ... 1 % MEM non-essential amino acid solution (N2 basal medium, all Thermo Fisher Scientific), 10 µmol/L CHIR99021 (Stemgent) ...
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bioRxiv - Zoology 2024Quote: ... Insect cells medium was also supplemented with 1 % non-essential amino acid (Gibco, France). Mosquito cells were grown at 28°C and mammalian cells at 37°C ...
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bioRxiv - Microbiology 2023Quote: ... supplemented with 10% Foetal bovine serum (FBS) and 1% non-essential amino acids (Gibco). Primary human neurons (HN ...
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bioRxiv - Biochemistry 2023Quote: ... Samples were resuspended in 50 µL 0.1% formic acid (FA) (LS118-1, Fisher Scientific) and diluted with 50 µL of 0.1% trifluoroacetic acid (TFA ...
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bioRxiv - Immunology 2024Quote: ... and 1% MEM non-essential amino acid solution (all Thermo Fisher Scientific, Scoresby, Australia). RPMI-7951 cells were maintained in RPMI-1640 medium supplemented with 10% fetal bovine serum (FBS) ...
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bioRxiv - Bioengineering 2020Quote: ... (anti-Claudin-1, anti-Claudin-5, anti-Occludin, anti-ZO-1; Invitrogen), in a 1:100 dilution in 10% donkey serum in PBS with 0.1% Saponin and incubated overnight on the Brain-Chip at 4°C ...
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bioRxiv - Genetics 2019Quote: ... and 5 µl of protein A: protein G 1:1 Dynabeads (Thermofisher). Antibody-bond chromatin was washed four times in low salt buffer (20 mM Tris-HCl pH 8.0 ...
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bioRxiv - Developmental Biology 2023Quote: ... Cludin-5 (1:1000) or -γ-tubulin (1:3000, Thermo Fisher Scientific) in Tris-buffered saline (Tris 50 mM and NaCl 150 mM ...
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bioRxiv - Synthetic Biology 2023Quote: ... 1 μL of T4 DNA ligase (5 U μL−1, ThermoFisher Scientific) and 2 μL of Milli-Q H2O (adjusted as needed to reach the total volume) ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... the cell layers are examined for cell morphology using a phase contrast microscope washed with media and then 10 uM of 2-(N-[7-nitrobenz-2-oxa-1, 3-diazol-4-yl] amino)-2-deoxyglucose (2-NBDG) (Molecular Probes-Invitrogen, CA, USA) was used to assess glucose uptake in the presence or absence of 100 nM insulin as the initiating step and incubated for 20 or 30 minutes ...
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bioRxiv - Immunology 2021Quote: ... were cocultured at 1:1 ratio for 6 days with anti-CD3/CD28–coated beads (Dynabeads, Life Technologies) at 1 bead ...
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bioRxiv - Cell Biology 2022Quote: ... For the bulk endocytosis experiments using the 4-[6-[4-(diethylamino)phenyl]-1,3,5-hexatrien-1-yl]-1-[3-(triethylammonio)propyl]-pyridiniumbromide dye (FM 4-64, Invitrogen), the cells were prepared as previously described [41] ...
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bioRxiv - Cell Biology 2021Quote: ... and a mix of amino-acids (1X MEM Non-Essential Amino Acids and 1X MEM Amino Acids, both manufactured by Gibco R). For strains and plasmid construction cells were grown in LB ...
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bioRxiv - Biophysics 2021Quote: ... 1 mg/mL in 2:1 chloroform:methanol) is from Invitrogen (USA) and naphthopyrene from Sigma-Aldrich (St ...
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bioRxiv - Molecular Biology 2022Quote: ... x 5 mm 5 μm 100 Å and an Acclaim PepMap RSLC 75 μm x 50 cm 2 μm 100 Å (Thermo Scientific). The columns were installed on an Ultimate 3000 RSLCnano system (Dionex) ...
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bioRxiv - Molecular Biology 2020Quote: ... 2 µL of 5 mM aa-dUTP/dNTP mix (5 mM each dATP, dGTP and dCTP, 3 mM dTTP and 2 mM 5-(3-aminoallyl)-dUTP (Thermo Fisher Scientific, AM8439)) and 1.25 µL of 40 µM oligo 1 ...
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bioRxiv - Biochemistry 2024Quote: ... x 5 mm 5 μm 100 Å and an Acclaim PepMap RSLC 75 μm x 50 cm 2 μm 100 Å (Thermo Scientific). The columns were installed on an Ultimate 3000 RSLCnano system (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2024Quote: Total RNA extracted from BoDV-2-infected Vero cells was ligated with either 5’ adaptor oligoRNA (5’-CGACUGGAGCACGAGGACACUGACAUGGACUGAAGGAGUAGAAA (Thermo Fisher Scientific, USA; #L150201)) or 3’ adaptor oligoRNA (5’-GAAGAGAAGGUGGAAAUGGCGUUUUGG ...
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bioRxiv - Immunology 2024Quote: ... resuspended in NK cell medium (RPMI 1640 medium containing GlutaMAX, 10% FBS, 1% P-S, 1% MEM Non-Essential Amino Acids Solution and 1% Sodium Pyruvate, both from Thermo Fisher Scientific), filtered using 70µm cell strainers and counted just before further use in flow cytometry experiments or in vitro experiments.
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bioRxiv - Cell Biology 2022Quote: ... 1 μg of plasmid was used to transfect 750,000 cells in each well of a 6-well plate using Lipofectamine 2000 (5 μL; Thermo Fisher Scientific) in OptiMEM (400 μL ...
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bioRxiv - Molecular Biology 2020Quote: DR-U2OS and -HCT116 cells and EJ-U2OS and -HCT116 reporter cells were plated in 6 well plates and transfected with 5 μL Lipofectamine RNAiMAX transfection reagent (Thermo Fisher) and siRNAs (Invitrogen ...
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bioRxiv - Bioengineering 2022Quote: Primary neonatal cardiomyocytes were isolated from C57BL/6 mouse neonates on postnatal days 3-5 using the Pierce Primary Cardiomyocyte Isolation Kit (Thermo Fisher) as previously described (14) ...
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bioRxiv - Neuroscience 2019Quote: ... The medium was changed every day and the cells were passaged every 5-6 days using PBS without Calcium and Magnesium (Life Technologies).
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bioRxiv - Neuroscience 2020Quote: Spinal cord tissue from stage HH 25-26 (Day 5-6) chick embryos (Venkateshwara hatcheries Ltd.) were dissected out in L-15 medium (Gibco, HiMedia) with added 1x penstrep (Gibco) ...
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bioRxiv - Microbiology 2020Quote: ... HAP1 cells with a low passage number were seeded into 6 well microtiter plates at a density of 5 × 105 cells/well in IMDM medium (#21980032, ThermoFisher Scientific) containing 10 % FBS (Sigma Aldrich ...
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bioRxiv - Neuroscience 2020Quote: ... The medium was changed every day and cells were passaged every 5-6 days using PBS without Calcium and Magnesium (Life Technologies).
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bioRxiv - Biochemistry 2022Quote: ... 5 μl of each binding reaction was loaded directly onto a pre-run 6% acrylamide DNA retardation gel (Thermo Fisher Scientific) and electrophoresed at ≤20 V cm−1 at 4 °C ...
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bioRxiv - Genomics 2022Quote: ... 2.6 μL nuclease-free water and 5 μL cDNA template on the ABI Prism 7500 Fast Sequence Detection System (Life Technologies). Thermo-cycling conditions were as follows ...
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bioRxiv - Developmental Biology 2021Quote: ... transferred to 1.6 mL Eppendorf tubes and spun at 2000 rcf for 5 minutes at 4°C in an angle rotor (Thermo Scientific Fisher Heraeus Biofuge Primo R Centrifuge) ...
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bioRxiv - Neuroscience 2020Quote: ... All 27 samples from 3 groups and 6 GIS were divided and labeled using two sets of 5 mg 11 plex TMT reagents (Thermo Scientific A34808 ...
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bioRxiv - Cell Biology 2019Quote: ... was used to amplify two homology arms spanning exons 5 and 6 of PPP2R1A and cloned into a bacterial artificial chromosome (Life Technologies). The homology arms were cloned into vector pNX (Papi et al. ...
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bioRxiv - Cancer Biology 2021Quote: ... The fluorescent reaction was measured every 40 sec for 8 min and subsequent every 5 min for 6 times using a microplate reader (Thermo Scientific) with enzyme kinetics model ...
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bioRxiv - Molecular Biology 2022Quote: ... were transfected using Lipofectamine 3000 kit (7.5 µl Lipo 3000 and 5 µl P3000 reagents per 6-well; Thermo Fisher Scientific) according to the manufacturer’s instruction ...
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bioRxiv - Biochemistry 2022Quote: ICabs A4 and B7 were labelled with NHS-Rhodamine (5/6-carboxy-tetramethyl-rhodamine succinimidyl ester, ThermoFisher Scientific (catalogue no. 46406)) as described by the manufacturer ...
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bioRxiv - Cell Biology 2022Quote: ... 5×105 cells were seeded in 6 cm plates and transfected with siRNAs using Lipofectamine™ RNAiMAX Transfection Reagent (#13778030, Invitrogen) as per manufacturer protocol ...
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bioRxiv - Genetics 2022Quote: ... Quantitative expression analysis of Nos1ap was performed using mouse-specific TaqMan Gene Expression assay (Mm01290688_m1; mapping to exons 5 and 6) (Applied Biosystems, MA). Real-time qPCR was performed on a 7900HT Fast Real-Time PCR System or QuantStudio 5 Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Molecular Biology 2022Quote: ... The products were analyzed in a denaturing 6% polyacrylamide gel with a 5’-labeled RiboRuler Low Range RNA Ladder (Thermo Scientific) as size markers ...
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bioRxiv - Immunology 2022Quote: ... 100 μg of HDM or OVA were reconstituted in PBS with 0.1 M sodium bicarbonate at 1 mg/ml and mixed with 18 μg Texas Red-succinimidyl ester or 36 μg 5,(6)-TAMRA-succinimidyl ester (Life Technologies), respectively ...
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bioRxiv - Biochemistry 2023Quote: 1.3 million HEK293T cells were seeded in a 6 cm dish in 5 ml of DMEM [high glucose DMEM (Gibco, 12800), 3.7 g/L NaHCO3 ...
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bioRxiv - Cancer Biology 2024Quote: ... Human embryonic stem cell line H9 was cultured on the 6-well plate coated with the Vitronectin (5 μg/ml) and in the Essential 8 Flex medium (both Thermo Fisher). Cells were split every 7 days using 5-minute incubation with 0.5 mM EDTA-PBS (Thermo Fisher ...