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Citations for Thermo Fisher :
2851 - 2900 of 10000+ citations for 6 Chloropyrazolo 1 5 a pyridine 2 carboxylic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2023Quote: We performed experimental evolution using SM/5 media [56] (2 g glucose (Fisher Scientific), 2 g BactoPeptone (Oxoid) ...
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bioRxiv - Cell Biology 2023Quote: ... Cy-2- or Cy-5-conjugated goat anti-mouse secondary antibodies (Thermo Fisher Scientific) were used at a 1:750 dilution in 5% HIGS/PBS ...
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bioRxiv - Biophysics 2023Quote: ... 2’(3’)-O-(2,4,6-trinitrophenyl)-adenosine 5’-triphosphate (TNP-ATP) was purchased from Invitrogen as a trisodium salt and stored in the dark at -20 °C at 10 mg/ml_ ...
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bioRxiv - Genetics 2023Quote: ... 2×10^5 individualized iPSCs were resuspended in 10 μL Resuspension Buffer R (Invitrogen) containing CRISPR ribonucleoproteins (Cas9 nuclease +sgRNA ...
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bioRxiv - Neuroscience 2023Quote: ... then they were incubated for 2 h in 5% normal donkey serum (NDS, Gibco), 0.2% Triton-X100 in PBS to block nonspecific binding ...
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bioRxiv - Developmental Biology 2023Quote: Cell proliferation assays were conducted employing 5-Ethynyl-2′-deoxyuridine (EdU; ThermoFisher Cat# C10337), a thymidine analog ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... in an atmosphere of 5% CO2 in an automatised incubator (Cytomat 2, Thermo Fisher). All experimental replicates were performed on a different day ...
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bioRxiv - Immunology 2021Quote: ... EBM-2/EGM-2 medium was supplemented with puromycin (1 mg/ml, Gibco) for 3 d ...
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bioRxiv - Bioengineering 2021Quote: ... and non-essential amino acids (10 mM, glycine, L-alanine, L-asparagine, L-aspartic acid, L-glutamic acid, L-proline, L-serine; Gibco). The pre-incubated viruses were then applied to Vero-E6 cell monolayers ...
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bioRxiv - Systems Biology 2021Quote: Cells were grown in Iscove’s Modified Dulbecco’s Medium (IMDM) medium +10% FBS +1% Non Essential Amino Acids +1% PennStrep (Gibco). All transfections used the Invitrogen Neon electroporation machine using a 100 ul tip ...
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bioRxiv - Neuroscience 2021Quote: ... Values of Rmin were obtained in situ by incubating preparations in Ca2+-free HL6 with 1 mM EGTA and 100 mM BAPTA-AM (1% DMSO, 0.2% Pluronic Acid) for 20min (Cat.No. B6769; Invitrogen). Values of Rmax were obtained in situ by incubating preparations in HL6 containing 10 mM Ca2+ and 100 μM ionomycin (Cat.No ...
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bioRxiv - Cancer Biology 2022Quote: We purchased 6-mercaptopurine monohydrate (6-MP, #AC226520050) from Thermo Fisher.
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bioRxiv - Molecular Biology 2021Quote: ... Nucleic acids were stained using SYBR gold Nucleic Acid Gel Stain (Invitrogen) and visualized using Gel Doc™ XR+ (Bio-Rad) ...
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bioRxiv - Biochemistry 2022Quote: ... formic acid (FA) and trifluoroacetic acid (TFA) were purchased from Thermo Fisher Scientific (Waltham ...
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bioRxiv - Biochemistry 2020Quote: ... The nanoLC system was equipped with an Acclaim™ Pepmap™ 100 C18 nano-trap column (1×2 cm, 5 μm particle size, Thermo Scientific, USA) and an Acclaim™ Pepmap™ 100 C18 analytical column (length 15 cm ...
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bioRxiv - Neuroscience 2022Quote: ... slides were rinsed with 0.025% Triton X-100/1xTBS 2 × 5 min and incubated in goat anti-rabbit AlexaFlour488 secondary antibody (1:1000, Life Technologies, #A27034, Carlsbad, CA) at RT for at least 2 h followed by 4’,6-diamidino-2-phenylindol (DAPI ...
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bioRxiv - Immunology 2023Quote: ... and STX-S_pLenti at a ratio of 5:5:1 using Lipofectamine 3000 (ThermoFisher, L3000008) according to the manufacturer’s instruction ...
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bioRxiv - Neuroscience 2021Quote: ... bicinchoninic acid (Invitrogen 23225) assays were conducted to determine protein concentrations ...
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bioRxiv - Microbiology 2021Quote: ... nonessential amino acids (Gibco), 2 mM L-glutamine (Invitrogen) ...
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bioRxiv - Cell Biology 2021Quote: ... casamino acids (ACROS Organics) and appropriate antibiotics when needed ...
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bioRxiv - Developmental Biology 2022Quote: ... nonessential amino acids (Gibco), L-glutamine (Gibco) ...
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bioRxiv - Molecular Biology 2021Quote: ... formic acid (Fisher Scientific), sequencing grade trypsin (Promega) ...
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bioRxiv - Immunology 2020Quote: ... nonessential amino acids (Gibco), 2-mercaptoethanol (Gibco) ...
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Individual kinetochore-fibers locally dissipate force to maintain robust mammalian spindle structurebioRxiv - Cell Biology 2019Quote: ... nonessential amino acids (Invitrogen), penicillin/streptomycin ...
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bioRxiv - Microbiology 2021Quote: ... nonessential amino acids (Gibco), 2 mM L-glutamine (Invitrogen) ...
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bioRxiv - Genomics 2024Quote: ... acid-phenol-chloroform (ThermoFisher) was used instead of chloroform.
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bioRxiv - Genetics 2023Quote: ... nonessential amino acids (Gibco), L-glutamine (Gibco) ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... nonessential amino acids (Gibco), and 2 mm L-glutamine (Gibco) ...
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bioRxiv - Cell Biology 2023Quote: ... 1mM pyruvic acid (Gibco) and 33mM glucose (Gibco)] for 2-4 hours ...
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bioRxiv - Neuroscience 2023Quote: ... nonessential amino acids (Thermofisher), B27 supplement (Thermofisher) ...
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bioRxiv - Biophysics 2023Quote: ... nonessential amino acids (Gibco), P-mercaptoethanol (Gibco) ...
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bioRxiv - Genomics 2023Quote: ... nonessential amino acids (Gibco) and penicillin/streptomycin (Gibco).
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bioRxiv - Immunology 2023Quote: ... Trifluoracetic acid (Fisher Scientific) at 10% sample volume was added ...
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bioRxiv - Genomics 2023Quote: ... nonessential amino acids (GIBCO), and 1,000 U/mL LIF (Millipore ...
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bioRxiv - Cell Biology 2021Quote: ... cells were pulsed with 10µM 5-Ethynyl-2’deoxyuridine (EdU) for 2 hours following manufacturer’s instructions (Click-iT® EdU Alexa Fluor® Imaging Kit, Life Technologies). Cells were subsequently fixed and stained with Hoechst 33342 ...
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bioRxiv - Physiology 2021Quote: ... HUVECs were washed 2 × with D-PBS and loaded with DAF-FM™ diacetate (4-amino-5-methylamino-2′,7′-difluorofluorescein diacetate; Molecular Probes, Invitrogen) to a final concentration of 1 µM in KRH buffer and incubated at 37°C for 45 minutes protected from light ...
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bioRxiv - Biophysics 2024Quote: ... Cell detachment for cryopreservation or experiments was achieved through 30 minutes of incubation with 5 mM EDTA in PBS without Ca+2 and Mg+2 (PBS-/-) followed by TrypLE Express dissociation agent (Gibco, 12605-010) for five minutes at 37°C ...
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bioRxiv - Cell Biology 2022Quote: ... Samples were loaded onto a 2 cm pre-column (C18, 5 μm, 100 A°, 100 μ, 2 cm Nano-viper column # 164564, Thermo Scientific) at 5 μl/minute flow rate using loading pump for about 5 minutes and then resolved the peptides on a 50 cm analytical column (C18 ...
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bioRxiv - Cell Biology 2023Quote: ... Treatments were incubated 2 hours before addition of the MTS (3-(4,5-Dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium) reagent (Thermo-Fisher Scientific, Waltham, MA) and incubation was continued another 1.5 hours ...
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bioRxiv - Immunology 2021Quote: ... was added as the secondary antibody at a 1:2000 dilution for 1 h at 37C, followed by adding TMB (3, 3, 5, 5’-tetramethylbenzidine) peroxidase substrate (Thermo Scientific) for about 15 min ...
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bioRxiv - Genetics 2019Quote: ... resuspended in 60 ml hypotonic lysis buffer (20 mM HEPES-KOH pH 7.5, 5 mM NaCl, 1 mM MgCl2, 1 mM PMSF and EDTA free protease inhibitor mixture from Roche and Thermo Scientific) and incubated for 15 min on ice ...
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bioRxiv - Genetics 2021Quote: ... The membrane was hybridized with a biotin-conjugated telomere probe (5’-biotin-CACACCCACACCCACACC-3’) and was imaged using a Chemiluminescent Nucleic Acid Detection Module Kit (Thermo Scientific) and a Li-Cor C-DiGit Chemiluminescent Western Blot scanner.
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bioRxiv - Immunology 2019Quote: ... Blood was deposited in tubes that contained 5 μL of 500 mM ethylenediamine tetraacetate acid (EDTA, Fisher Scientific, Fair Lawn, NJ).
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bioRxiv - Cancer Biology 2020Quote: ... Neutrophils were treated for 3h at 37°C and 5% CO2 and then stained with 20uM SYTOX(tm) Green Nucleic Acid Stain for 10min (Thermofisher Scientific). Images and videos were taken using a Zeiss Axio Observer.Z1 coupled with incubation chamber (ZEISS) ...
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bioRxiv - Biochemistry 2021Quote: ... and proteins were separated using a gradient of solvent B (99% acetonitrile with 0.1% formic acid) from 5 to 65% in 90 minutes using an EASY-nLC 1000 system (Thermo Fisher Scientific). Ion fragmentation was conducted using collision-induced dissociation (CID) ...
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bioRxiv - Cell Biology 2021Quote: ... the peptides were resuspended in 3% formic acid (FA)/5% acetonitrile (ACN) and loaded onto a nanoLC system (RSLCnano, Thermo Scientific). First ...
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bioRxiv - Plant Biology 2022Quote: SepPak eluted peptides were topped up to 65% (v/v) acetonitrile (ACN) and 5% (v/v) trifluoroacetic acid (TFA) prior to enrichment using a KingFisher Duo (Thermo Scientific) automated sample preparation device as outlined by Leutert et al ...
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bioRxiv - Biochemistry 2021Quote: ... with 0.05% (v/v) trifluoroacetic acid (5 min 20 µL/min) using split-free UltiMate 3000 RSLCnano HPLCs (Dionex / Thermo Scientific, Germany). Bound peptides were then eluted with an aqueous-organic gradient (eluent A ...
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bioRxiv - Plant Biology 2019Quote: The peptides were dissolved in 5 μL of 0.3% formic acid with 3% acetonitrile and injected into an Easy-nLC 1200 (Thermo Fisher Scientific). Peptides were separated on a 25 cm Easy-Spray column (75 μm ID ...
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bioRxiv - Neuroscience 2022Quote: ... Eluted peptides were dried down overnight before resuspension in 5% formic acid ready for injection onto the Orbitrap Exploris (Thermo Fisher) mass spectrometer using data independent acquisition (DIA) ...