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Citations for Thermo Fisher :
2901 - 2950 of 10000+ citations for 6 Chloropyrazolo 1 5 a pyridine 2 carboxylic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... gels were stained by incubation in 50 ml ddH20 containing 5 μl SYBR® Gold Nucleic Acid Gel Stain (Invitrogen, S11494) on a rocking platform for 30 min ...
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bioRxiv - Neuroscience 2022Quote: ... slices were transferred into a sealed 1.5 ml tube containing 5 µM Fluo-4AM (Molecular Probes, 50% DMSO, 50% Pluronic Acid) diluted in oxygenated modified artificial cerebrospinal fluid (mACSF ...
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bioRxiv - Cancer Biology 2024Quote: ... Each sample was then resuspended in a volume of sample LC/MS loading solvent [5% formic acid (Optima grade, ThermoFisher Scientific) and 4% acetonitrile (both water and acetonitrile were LC-MS-grade ...
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bioRxiv - Cell Biology 2024Quote: ... protocol and these dry peptides residues were solubilized in 20 µL 5 % acetonitrile with 0.5 % formic acid using the auto-sampler of a nanoflow uHPLC system (Thermo Scientific RSLCnano). Online detection of peptide ions was by electrospray ionisation (ESI ...
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bioRxiv - Synthetic Biology 2023Quote: ... 5 g/L ammonium sulphate (Fisher Chemical™) and 1.7 g/L yeast nitrogen base without amino acids (Thermo Scientific™) and was supplemented with 2% (w/v ...
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bioRxiv - Biochemistry 2023Quote: ... the sample was acidified with formic acid (5% final concentration) and fractionated using high pH reversed-phase peptide fractionation kits (ThermoFisher, 84868) according to Vinogradova and coworkers (Cell ...
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bioRxiv - Cell Biology 2023Quote: For flowcytometric quantification of GFP signal in induced 3D7-DiCre pFIO/pFIO+ strains 2.5 µl iRBCs were washed once with RT 0.9% NaCl solution (B. Braun GmbH) and stained with 5 µM SYTO 61 Red Fluorescent Nucleic Acid stain (Thermo Fisher Scientific) for 1 h at 37°C in 0.9% NaCl solution and washed once again afterwards at RT ...
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bioRxiv - Cancer Biology 2024Quote: ... in the extracellular domain of the CaSR at a concentration of 5 μg/ml and counterstained with SYTO13 fluorescent nucleic acid dye (ThermoFisher, S7575) as previously described 33 ...
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bioRxiv - Immunology 2023Quote: ... A 5-fold excess of pre-warmed RPMI+ medium (RPMI-1640, 100 μM non-essential amino acids, 20 mM HEPES [Thermo Fisher Scientific] ...
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bioRxiv - Cell Biology 2024Quote: ... TMB substrate (Thermo 34028) was added for approximately 5 minutes and reaction was stopped with sulfuric acid Stop solution (Invitrogen, SS04). Absorbance was immediately read at 450 nm ...
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bioRxiv - Molecular Biology 2020Quote: ... The dried peptides were taken up in 0.1% (v/v) formic acid and then loaded onto a PepMap100 C18 LC pre-column (75 μm I.D., 2 cm, Thermo Scientific) and eluted on line onto an analytical NanoViper PepMap 100 C18 LC column (75 μm I.D. ...
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bioRxiv - Molecular Biology 2020Quote: ... at 200 V for 40 min in NuPAGE 2-(N-morpholino)ethanesulfonic acid (MES) running buffer (cat. no. NP0002, Invitrogen), supplemented with NuPAGE antioxidant (cat ...
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bioRxiv - Immunology 2022Quote: ... ileal tissue was collected and stored in stabilization buffer (2 mM ethylenediaminetetraacetic acid [EDTA; Invitrogen AM9260G], 2mM L-glutamine [Gibco 25-030] ...
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bioRxiv - Immunology 2019Quote: ... and eluted using a 500 μl/min flow rate over 10 min from 2 % buffer A to 35 % buffer B (0.1% formic acid in acetonitrile) with an Ultimate 3000 HPLC system (Thermo Scientific). One ml fractions were collected from 2 to 15 min ...
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bioRxiv - Molecular Biology 2019Quote: ... We measured non-esterified fatty acids with HR Series NEFA-HR(2) (Wako Pure Chemicals) and triglycerides with Infinity #TR22421 (ThermoFisher).
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bioRxiv - Microbiology 2019Quote: ... Peptides from each fraction were reconstituted in 0.1% formic acid and loaded on a pre-column (PepMap C18, 100 μm × 2 cm, Thermo Scientific) at a flow rate of 5 μL per min ...
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bioRxiv - Pathology 2019Quote: ... or a custom diet high in fat (60 kcal%), cholesterol (1.25%) and cholic acid (0.5%) (D11061901, Research Diet, Denmark) as well as 2% CDX (Fisher Scientific, France) in drinking water (HFCC-CDX group).
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bioRxiv - Bioengineering 2020Quote: The quantification of BMP-2 initially loaded in the polyelectrolyte film (deposited at the bottom of a 96-well microplate) was done using a micro bicinchoninic acid assay (microBCA) test for low BMP-2 concentration (BMP20) while Nanodrop (Thermofisher) was used for higher concentrations ...
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bioRxiv - Bioengineering 2021Quote: ... T cells were cultured at 2×106 cells/ml in T cell media (R10 supplemented with 1X non-essential amino acids (Gibco) + 1 mM sodium pyruvate (Gibco ...
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bioRxiv - Biochemistry 2022Quote: ... the samples were re-suspended in 2 % formic acid and analysed using an UltiMate™ 3000 RSLCnano System (ThermoFisher Scientific) coupled on-line to an Orbitrap Exploris 480 (ThermoFischer Scientific) ...
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bioRxiv - Genetics 2022Quote: ... The peptides were dissolved in 2% formic acid and analysed on a Q-Exactive HF mass spectroscopy (Thermo Fisher Scientific). The peptides were separated using an EASY-Spray PepMap RSLC C18 column at flow rate of 300 nL/minute on a 60 min linear gradient of the mobile phase (5% acetonitrile (ACN ...
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bioRxiv - Microbiology 2021Quote: Multiplexed peptide fractions from each time point were resuspended in mobile phase A solvent (2% acetonitrile and 0.1% formic acid) to be analyzed on the Exploris 480 mass spectrometer equipped with FAIMS (ThermoFisher Scientific). The mass spectrometer was interfaced to the Easy nanoLC1200 HPLC system (ThermoFisher Scientific) ...
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bioRxiv - Immunology 2019Quote: Samples were diluted in culture medium (RPMI 1640 [Gibco, Massachusetts/USA] supplemented with 2 g of NaHCO3, 10 mL of nonessential amino acids [Gibco] ...
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bioRxiv - Biochemistry 2020Quote: ... The liquid chromatography used a 2-90% MeCN linear gradient in water with 0.1% formic acid on a C18 BioBasic analytical HPLC column (Thermo Scientific) installed on an Agilent 1290 HPLC system ...
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bioRxiv - Biochemistry 2021Quote: We monitored the change in absorbance over time of 5,5’-dithiobis-(2-nitrobenzoic acid) (DTNB, Ellman’s reagent, Thermo Fisher Scientific). Various concentrations of DTNB were added to protein and change in absorbance was measured in either an SX-20 Stopped Flow instrument (Applied Photophysics ...
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bioRxiv - Immunology 2020Quote: ... the desalted samples were first resuspended into 20 μl 0.1% formic acid in water and then loaded onto a trap column (2 cm × 300 μm, PepMap C18, Thermo Scientific) and an analytical column (19 cm × 75 μm ...
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bioRxiv - Molecular Biology 2020Quote: ... peptides were trapped at 3 μl/min on a 10 mm × 75 μm ID Acclaim Pepmap trap at 2% buffer B (buffer A: 0.1% formic acid (Fisher Scientific), buffer B ...
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bioRxiv - Molecular Biology 2022Quote: ... Peptides were then dissolved in 2% ACN and 0.1% formic acid and analyzed on a Thermo Scientific Q Exactive Mass Spectrometer (ThermoFisher Scientific). The MS was operated in data-dependent mode ...
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bioRxiv - Molecular Biology 2022Quote: The reconstituted peptides in solvent A (2% acetonitrile, 0.1% formic acid) were separated on a 50 cm long EASY-spray column (ThermoFisher Scientific) connected to an Ultimate-3000 nano-LC system (ThermoFisher Scientific ...
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bioRxiv - Molecular Biology 2024Quote: ... 0.1% formic acid in acetonitrile) on an EASY-Spray column ES801 (50 µm ID × 15 cm, 2 µm; Thermo Scientific) mounted on a DPV ion source (New Objective ...
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Macropinocytosis mediates resistance to loss of glutamine transport in triple-negative breast cancerbioRxiv - Cancer Biology 2024Quote: ... Cells were trypsinised and resuspended in FACS buffer (1ξ Ca2+ and Mg2+ free PBS, 2 mM ethylenediaminetetraacetic acid (Life Technologies), 2% FBS Bovogen) ...
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bioRxiv - Cancer Biology 2024Quote: Peptides were reconstituted with 2% formic acid and analyzed in triplicates on an Orbitrap Exploris 480 mass spectrometer (Thermo Scientific) coupled to an UltiMate 3000 UHPLC system (Thermo Scientific) ...
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bioRxiv - Physiology 2024Quote: ... fractions were resuspended in 2% acetonitrile and 0.1% formic acid and separated on an Easy nLC 1200 (Thermo Fisher Scientific) and a 35 cm long ...
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bioRxiv - Cancer Biology 2024Quote: ... 100 µM × 250 mm) in Solvent A (98% water, 2%acetonitrile, 0.1% formic acid) via a Nanospray Flex Ion-Source (Thermo Scientific) at a voltage of 1.9 kV with a flow rate of 0.7 µL/min in Solvent A (98% water/2%acetonitrile/0.1% formic acid) ...
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bioRxiv - Systems Biology 2023Quote: ... A 1 mL aliquot of 2:2:1 (v/v) mixture of ≥ 99.9% purity acetonitrile (Fisher Scientific; Cat.A998-4) ≥ 99.8% purity methanol (Fisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... for RoNi/7.1 cells and 1% Non-Essential Amino Acids (NEAA; Thermo Fisher Scientific) for Huh-7 and RoNi/7.1 cells ...
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bioRxiv - Microbiology 2021Quote: ... the chamber was precoated with 1 % poly L-glutamic acid (Fisher Scientific, P4886-25MG) for one hour and washed twice with 70 % EtOH ...
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bioRxiv - Plant Biology 2019Quote: ... in hydrochloric acid 0.01 N (1:3, v:v) (Fisher Scientific; Hampton, New Hampshire, USA).
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bioRxiv - Plant Biology 2020Quote: ... before adding 500 μL of Acid Phenol:Chloroform:IAA (125:24:1) (Cat No.: AM9720, Thermofisher). The tubes were shaken in a SPEX CertiPrep 2010-230 Geno/Grinder for 10 min at 500 rpm ...
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bioRxiv - Microbiology 2019Quote: ... Parasites were stained for nucleic acid content with 1 μM SYTO 61 (Life Technologies) and analyzed in the FL4 signal channel to determine the fraction of parasitized red blood cells (parasitemia).
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bioRxiv - Microbiology 2020Quote: ... cells were incubated with 1 mM SYTOX Orange Nucleic Acid Stain (Molecular Probes; S11368) for 15 min in the dark at room temperature to stain for NETs ...
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bioRxiv - Cell Biology 2021Quote: ... Acid-solubilized bovine collagen 1 (Devro) was salt balanced using 10x DMEM (ThermoFisher Scientific) and pH neutralized using 0.1M NaOH before combining with Matrigel and then the cell suspension on ice ...
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bioRxiv - Cell Biology 2021Quote: ... 100 U/mL P/S and 1 × non-essential amino acids (NEAA, 11140035, Gibco). Transfections were performed using the Neon Transfection System (Invitrogen) ...
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bioRxiv - Cancer Biology 2020Quote: ... and 1% (v/v) MEM Non-essential Amino Acids (Gibco, Grand Island, NY, USA). All cell lines were cultured at 37°C in a humidified incubator with 5% CO2.
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bioRxiv - Cancer Biology 2021Quote: ... 1% (v/v) MEM non-essential amino acid solution and 1mM sodium pyruvate (Thermofisher), hereafter referred as TBM media ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Formic acid (99+%) was purchased in 1 mL ampules from Thermo Scientific (Rockford, IL). Ammonium formate and 2-propanol (both Optima® LC/MS grade) ...
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bioRxiv - Molecular Biology 2019Quote: ... 1% MEM Non-Essential Amino Acids Solution (NEAA 100X, Gibco, Thermo Fisher Scientific Inc.) and Gentamicin (50 µg/mL ...
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bioRxiv - Molecular Biology 2019Quote: ... 1% MEM Non-Essential Amino Acids Solution (NEAA 100X, Gibco, Thermo Fisher Scientific Inc.) and Gentamicin (50 µg/mL ...
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bioRxiv - Cell Biology 2020Quote: ... and 1 µL of GlycoBlue nucleic acid co-precipitant (15 mg/mL; Thermo Fisher) were added ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... and 1 mL ∼30% hydrochloric acid (TraceMetal™ Grade, Thermo Fisher Scientific, Waltham, MA) overnight ...