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Citations for Thermo Fisher :
2951 - 3000 of 10000+ citations for 6 1 4 Dioxa 8 azaspiro 4.5 dec 8 yl 3 pyridinyl boronic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... and WI-38 VA-13 cells were cultivated in 4.5 g L−1 Dulbecco’s modified eagle medium (DMEM) supplemented with 10% fetal bovine serum (Gibco), 2 mM glutamine (Thermo Scientific) ...
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bioRxiv - Cancer Biology 2019Quote: ... All TaqMan probes were 5′-6-carboxyfluorescein (FAM) and 3′-6-carboxy-N,N,N′,N′-tetramethylrhodamine (TAMRA) labeled (Applied Biosystems, US) except TATA-binding protein (TBP ...
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bioRxiv - Biophysics 2024Quote: ... and 1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydro (EDC, ThermoFisher). For BS3 crosslinking ...
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bioRxiv - Bioengineering 2024Quote: ... NK cells and target cells were mixed at an effector to target (E/T) ratio of 4:1 (SK-BR-3 cells) or 2:1 (K562 cells) and Sytox Green (Thermo Fisher Scientific) was added at 100 nM ...
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bioRxiv - Cell Biology 2022Quote: ... DNP (EM 1:6-1:30, #71-3500, Invitrogen), DPP4 (IF/FACS 1:100 ...
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bioRxiv - Immunology 2021Quote: ... Peptide and enzyme solutions were dissolved and diluted in 25 mM 4-(2-hydroxyethyl)-1-piperazine ethanesulfonic acid (HEPES, Fisher Scientific). The peptide (100 μM in a total volume of 100 μL ...
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bioRxiv - Cancer Biology 2021Quote: ... slides were incubated overnight in a humidified chamber at 4 °C in TBS-T with 1% Fatty acid free-BSA (Fisher Scientific) with anti-mouse/human antibodies ...
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bioRxiv - Cancer Biology 2022Quote: ... The stock solutions of the agonists were made in 25 mM 4-(2-hydroxyethyl)-1-piperazine ethanesulfonic acid (HEPES, Fisher Scientific) and the enzyme inhibitors were reconstituted according to manufacturers’ instructions ...
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bioRxiv - Cell Biology 2024Quote: ... The samples were dissolved in 10 µL of reconstitution buffer (96:4 water: acetonitrile, 1% formic acid and analyzed by LC-MS/MS using QExactive (Thermo Fisher) in the proteomics core facility at EMBL Heidelberg (https://www.embl.org/groups/proteomics/).
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bioRxiv - Cell Biology 2024Quote: ... The samples were dissolved in 10 µL of reconstitution buffer (96:4 water: acetonitrile, 1% formic acid and analyzed by LC-MS/MS using QExactive (Thermo Fisher) in the proteomics core facility at EMBL Heidelberg (https://www.embl.org/groups/proteomics/) ...
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bioRxiv - Immunology 2023Quote: ... The WIV was then dialyzed against 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES)-buffered saline (Thermo Fisher Scientific, Bleiswijk, the Netherlands) at 4°C overnight to remove β-propiolactone ...
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bioRxiv - Cell Biology 2020Quote: ... Single cell suspension of these reporter mESCs were plated in Nunc 8-well chambered cover-glass slides (Thermo Fisher) coated with 2.5μg/cm2 human fibronectin at 104 cells per well density ...
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bioRxiv - Developmental Biology 2021Quote: ... was cultured at 2-8×105 cells/ml in Fischer’s medium with 20% horse serum and 2 mM L-glutamine (all Gibco).
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bioRxiv - Developmental Biology 2021Quote: ... Samples on day 8 or 10 were dissociated with enzyme mix composed of 0.25% Trypsin (Thermo Fisher Scientific, 25200056), and 0.05×DNaseI (STEMCELL Technologies ...
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bioRxiv - Neuroscience 2022Quote: C57BL/6J mice (8-14 weeks) were injected with CypHer-Aβ (ipsilateral) and NHS-Fluorescein (contralateral, Thermo Fisher 46410) as previously described60 ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were settled onto 8-well glass chamber slides (Nunc™ Lab-Tek™ II Chamber Slide™ System) and stained according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... Between 8-15 μg of DNA was incubated with 100 μl (~10 mg) of Dynabeads MyOne Streptavidin C1 (Invitrogen) for 15 min with rotation ...
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bioRxiv - Molecular Biology 2021Quote: ... and the C-terminal foldon trimerization motif followed by an 8×His-tag was cloned into the pcDNA3.1(+) expression vector (Invitrogen). Furthermore ...
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bioRxiv - Evolutionary Biology 2020Quote: We mixed 0.8 μl of PCR product with 8.8 μl of formamide and 0.4 μl of marker Liz 500 (Applied Biosystems). We heated the mixture to 95°C for 5 min and let it cool down ...
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bioRxiv - Cell Biology 2019Quote: ... 60 000 cells/ml were seeded in 8-well Nunc™ Lab-Tek™ II Chambered Coverglass (Thermo Fisher) and differentiated for 5-7 days by adding 10 μM all-trans retinoic acid (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2020Quote: Oligonucleotides were supplied PAGE-purified by IDT and resuspended to 100 μM in TE buffer pH 8 (Invitrogen, #AM9849). Equimolar amounts were mixed in 200 μL aliquots at 40μM in TE supplemented with 50 mM NaCl ...
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bioRxiv - Cancer Biology 2019Quote: ... 5-8×104 cells in 100 μL of 0.9x PBS were loaded into EZ Single Cytofunnel (ThermoFisher Scientific A78710003) containing coated Shandon Cytoslides (ThermoFisher Scientific 5991056 ...
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bioRxiv - Genomics 2021Quote: ... we seeded cells onto Geltrex-coated Nunc Lab-Tek 8-well chambered coverglass (Thermo Scientific, cat. 12-565-470) at a density of 4-5×104 cells per well ...
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bioRxiv - Neuroscience 2020Quote: ... primary DP cells from P5-8 Tgfbr2fl/fl molars were grown in alpha-minimum essential medium (alpha-MEM; Gibco) supplemented with 10% heat-inactivated fetal bovine serum ...
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bioRxiv - Bioengineering 2021Quote: A standard endodermal differentiation protocol calls for HUES-8 spheroids to be cultured in MCDB131 medium (Thermo Fisher Scientific) containing 200 ng/mL Nodal or 100 ng/mL Activin A (both obtained from R&D Systems ...
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bioRxiv - Biochemistry 2020Quote: ... and 8 pmol of purified DNA was then used for in vitro transcription with T7 TranscriptAid kits (Thermo Scientific). The resulting RNA was purified with Agencourt RNAClean XP beads supplemented with an additional 12% of PEG-8000 and quantified via NanoDrop ...
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bioRxiv - Bioengineering 2021Quote: The tissue was washed with 40 μl 1X RT buffer containing 8 μl Maxima 5x RT Buffer (EP0751, Thermofisher), 1 μl RNase Inhibitor (30281 ...
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bioRxiv - Microbiology 2020Quote: Human ileocecal adenocarcinoma cells (HCT-8 cells, ATCC CCL-244) were maintained in RPMI 1640 medium (Gibco, ATCC modification) supplemented with 10% fetal bovine serum ...
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bioRxiv - Microbiology 2020Quote: Cells seeded into 8-chamber culture slides (Nunc™ Lab-Tek™ II Chamber Slide™ System, ThermoFisher Scientific) (0.4×106 cells/well ...
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bioRxiv - Cell Biology 2021Quote: WA09 (H9) hESC line was purchased from WiCell (wicell.org) and maintained in Essential 8 medium (A151700, Thermo Fisher Scientific) on hESC-qualified growth factor reduced Geltrex-coated (A1413302 ...
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bioRxiv - Cell Biology 2021Quote: ... cells were passaged (0.05% Trypsin EDTA, #25300–054, Gibco, Waltham, MA and 5mg/mL soybean Trypsin inhibitor, #9035-81-8, Gibco) and seeded at 4,000 cells/cm2 into 1× 60 mm and 1× 35 mm collagen-coated culture dishes ...
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bioRxiv - Microbiology 2022Quote: ... pH 8) at room temperature in PierceTM Slide A Lyzer® Mini Dialysis Units (Thermo Fisher Scientific, Waltham, MA). Turbidity measurements at A340 nm were collected in order to quantify the reaction progress by using NanoDrop 1000 Spectrophotometer (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2022Quote: ... HCT-8 cells were cultured in 25 cm flasks with 5 ml of complete media (RPMI-1640 media [Gibco/Thermo Fisher Scientific ...
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bioRxiv - Biophysics 2022Quote: ... at 37°C and 8% CO2 to a density of 3.5×106 cells/mL in FreeStyle suspension media (Gibco) supplemented with 2% fetal bovine serum (Gibco ...
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bioRxiv - Cell Biology 2022Quote: ... 10 μl of the supernatant was used for SDS-PAGE on 8-12% Tris-Glycine gels (NOVEX™, Invitrogen) with Tris-Glycine SDS Running buffer (NOVEX™ ...
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bioRxiv - Cell Biology 2022Quote: ... livers were perfused in situ with Hank’s balanced salt solution without calcium containing 8 mM HEPES (Thermo Fisher Scientific) via the inferior vena cava after cutting the portal vein to allow outflow of the perfusate ...
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bioRxiv - Neuroscience 2021Quote: ... 8% SDS and 2% β-mercaptoethanol) were loaded onto 12% SDS-polyacrylamide gel and See-Plus 2 (Invitrogen, LC5925) was used as a molecular-weight marker ...
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The cellular basis of protease activated receptor type 2 (PAR2) evoked mechanical and affective painbioRxiv - Neuroscience 2020Quote: DRG cultures were prepared as described with the neurons plated on 8-well Chamber Slides (154534; Nunc Lab-Tek) coated with poly-D-lysine (P0899 ...
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bioRxiv - Neuroscience 2021Quote: ... HeLa digest was fractionated into 8 fractions using a high pH reversed-phase peptide fractionation Kit (ThermoFisher, Cat. #84868). Twenty μg of HeLa digest was loaded to a C18 solid phase spin column and eluted with a serial concentration of ACN (5% to 50%) ...
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bioRxiv - Plant Biology 2021Quote: ... and PpEF1α were cloned into pCR™8/GW/TOPO™ or pCR™-Blunt (Thermo Fisher Scientific, Inc.). To analyze virulence and protein accumulation in N ...
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bioRxiv - Neuroscience 2020Quote: ... The individual colonies were picked to 24-well matrigel-coated plate containing Essential 8 Medium (E8, Thermo Fisher Scientific) between days 17-28 and passaged with 0.5 mM EDTA weekly ...
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bioRxiv - Neuroscience 2020Quote: ... of brain grey matter tissue was homogenized in 500 μL of 8 M urea lysis buffer (8 M urea, 100 mM Na2HPO4, pH 8.5) with HALT protease and phosphatase inhibitor cocktail (ThermoFisher) using a Bullet Blender (NextAdvance) ...
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bioRxiv - Microbiology 2022Quote: Between 7×104 and 1.2×105 HepG2 cells (ATCC) per well were seeded in an 8-well Lab-Tek chamber slide (Nunc), precoated with rat tail collagen type I (Sigma ...
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bioRxiv - Microbiology 2022Quote: ... interferon-β treated (250 U/ml for 8 h) or infected with influenza WSN (MOI 0.02 for 24 h) using TRIzol (Invitrogen). Biologic triplicate sample RNA was sent for library preparation and paired-end RNA-seq by Novogene (BioProject PRJNA667475) ...
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bioRxiv - Neuroscience 2019Quote: Single-molecule FRET measurements were carried out using ~30 pM of labelled tau in phosphate buffer (40 mM potassium phosphate, 50 mM KCl, pH 7.4) in 8-chambered Nunc coverslips (ThermoFisher) passivated with poly(ethylene glycol ...
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bioRxiv - Genetics 2019Quote: ... undifferentiated cells were grown in a feeder-free culture conditions using defined Essential 8 medium combined with vitronectin-(Gibco)-coated surfaces ...
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bioRxiv - Microbiology 2019Quote: ... 115 rpm and 8% CO2 following the Max Titer protocol with an additional feed on day five (Thermo Fisher). Culture supernatants were harvested 13 to 14 days after transfection ...
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bioRxiv - Cell Biology 2020Quote: ... Vector particles were produced from second day supernatants conditioned by 293FT cells transduced using 8 μg/ml polybrene (Invitrogen) (Szulc and Aebischer ...
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bioRxiv - Neuroscience 2021Quote: ... Indels were introduced by transfecting 8×105 cells with 1.5 μg plasmid in 100 μl scale using the Neon Transfection System (ThermoFisher) with one pulse at 1500 millivolts for 30 milliseconds (∼40-70% transfection efficiency) ...
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bioRxiv - Cell Biology 2020Quote: ... Gels were polymerized for 2 h at 37°C and then digested with 8 U/ml Proteinase K (Invitrogen) in 50 mM Tris-HCl pH 8 ...