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Citations for Thermo Fisher :
3001 - 3050 of 10000+ citations for 6 1 4 Dioxa 8 azaspiro 4.5 dec 8 yl 3 pyridinyl boronic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... On the 15th day of reprogramming N2B27 medium was switched to complete Essential 8 medium (E8; Thermo Fisher Scientific), which was then changed every two days ...
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bioRxiv - Molecular Biology 2021Quote: The droplets were re-injected in the first pico-injector device with the shorter re-injection channel (Figure S1C) and coalescence-induced merging with a poly(A) solution consisting of 26.6 mM Tris-HCl pH 8 (Invitrogen), 15.8 mM DTT (Invitrogen) ...
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bioRxiv - Microbiology 2021Quote: ... PCR products (~240-290 bp) were purified by gel extraction on an 8% TBE polyacrylamide gel (Thermo Fisher Scientific) and isopropanol precipitation ...
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bioRxiv - Biophysics 2021Quote: ... P2 viruses (8%) were used to infect tsA201 cells grown in Freestyle 293 Expression Medium in suspension culture (ThermoFisher). Infected cells were incubated for an initial 12 h at 37 °C before 10 mM sodium butyrate was added ...
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bioRxiv - Immunology 2020Quote: ... fraction was separated by centrifugation at 10,000 x g for 30 minutes at 2-8 °C (Sorvall Lynx 6000, ThermoFisher). The IB pellet was washed with 20 mM HEPES (pH 8.0) ...
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bioRxiv - Neuroscience 2019Quote: Induced pluripotent stem cells were maintained in E8™ medium with Essential 8™ supplement (Gibco-Thermo Fisher Scientific) and passaged once every 5-6 days using Dispase (StemCell Technology) ...
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bioRxiv - Neuroscience 2019Quote: Induced pluripotent stem cells were maintained in E8™ medium with Essential 8™ supplement (Gibco-Thermo Fisher Scientific) and passaged once every 5-6 days using Dispase (StemCell Technology) ...
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bioRxiv - Genetics 2020Quote: ... The PCR was performed in MicroAmp Fast Optical 96-Well Reaction Plates or Optical 8-Well Strips (Applied Biosystems) by first heating to 95°C for 5 minutes before running through 40 cycles of 95°C for 15 seconds ...
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bioRxiv - Cell Biology 2020Quote: ... Clinical hESCs were maintained in commercially available Essential 8™ (E8) basal medium (Gibco, Carlsbad, MO, USA; A15169-01) with Essential 8™ Supplement (Gibco ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Fibroblast chemotaxis was measured using 24-well Nunc (8-µm pore size) Transwell inserts (Thermo Fisher, Waltham, MA, USA). Following confluence ...
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bioRxiv - Molecular Biology 2021Quote: ... Lysosomal ExRai AMPKAR was made by inserting PCR-amplified LAMP149 generated using primers 7-8 into HindIII (ThermoFisher FD0504)/BamHI-digested ExRai AMPKAR backbone ...
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bioRxiv - Microbiology 2020Quote: Human ileocecal adenocarcinoma cells (HCT-8 cells; ATCC CCL-244) were cultured in RPMI 1640 medium (Gibco, ATCC modification) supplemented with 10% fetal bovine serum ...
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bioRxiv - Microbiology 2019Quote: Huh-7 and C6/36 cells were grown in 8 well chambers (LAB-TEK®, Nalgene Nunc International, USA) for 24h ...
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bioRxiv - Immunology 2020Quote: ... the implants were washed once with 1XPBS and placed in 8-well chamber slides (Thermo Fisher Scientific, Waltham, MA) (Figure 1) ...
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bioRxiv - Immunology 2021Quote: ... 8 µm sections were cut using a cryostat and sections were mounted on SuperFrost Plus glass slides (ThermoFisher Scientific). Sections were incubated in ice-cold acetone for 10 min prior to blocking in 5% BSA in PBS ...
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bioRxiv - Immunology 2020Quote: ... cells were quickly lysed in 8% Lithium dodecyl sulfate LDS loading buffer preheated at 85°C (Invitrogen Cat#NP0007) and incubated with stirring at 500 rpm for 5 min at 85°C (Eppendorf ...
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bioRxiv - Cell Biology 2019Quote: ... HeLa cells were seeded at 70% confluency in 8 well chambers and transfected with lipofectamine 2000 (ThermoFisher Scientific, 11668030). For RAW macrophages ...
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bioRxiv - Microbiology 2021Quote: ... 115 rpm and 8% CO2 following the Max Titer protocol with an additional feed on day five (Thermo Fisher). Culture supernatants were harvested 13 to 14 days after transfection ...
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bioRxiv - Immunology 2022Quote: ... Ampho cells were washed with OptiMEM and transfected with 8 ug of retroviral vector using Lipofectamine 3000 (16 μL) and PLUS reagent (40 μL) following manufacturer’s instructions (Invitrogen) 113 ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were grown in Essential 8 (made in-house based on (Chen et al., 2011)) on Vitronectin (Life Technologies), and were maintained at 37°C under a humidified atmosphere of 5% CO2 in air ...
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bioRxiv - Cancer Biology 2022Quote: HCI-EC-23 and Ishikawa cells were grown in regular media in 8-well chamber slides (Thermo Scientific, 177402PK). Cells were washed with 1x PBS and then fixed using 4% formaldehyde (Sigma Aldrich ...
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bioRxiv - Biochemistry 2022Quote: ... and were detached from the 8-well chamber slides using 100 μL Trypsin-EDTA (ThermoFisher Scientific, catalog no. 25200056). Cells were then resuspended using 100 μL of DMEM (Corning ...
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bioRxiv - Cell Biology 2022Quote: ... Cells used for microscopy were plated in LabTek 8 well dishes and were transiently transfected using Lipofectamine 3000 (ThermoFisher) using 2.5 mg/ml of CD63-pHluorin plasmid DNA (gift from D.M Pegtel27 ...
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bioRxiv - Genetics 2022Quote: ... 8 μl of the eluted DNA were transformed into 20 μl of electrocompetent cells (MegaX DH10B, Thermo Fisher Scientific) according to the manufacturer’s protocol using the ECM 399 electroporator (BTX) ...
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SMDT1 variants impair EMRE-mediated mitochondrial calcium uptake in patients with muscle involvementbioRxiv - Genetics 2022Quote: Fibroblasts were seeded in an 8-well glass-bottomed chamber (#155411; Nunc Lab-Tek Thermo Scientific, Waltham, MA, USA), three days prior to the experiment ...
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bioRxiv - Neuroscience 2022Quote: ... Brain tissues were lysed in 8 M urea lysis buffer (8 M urea, 10mM Tris, 100 mM NaH2PO4, pH 8.5) with HALT protease and phosphatase inhibitor cocktail (ThermoFisher). Homogenization was performed using a Bullet Blender (Next Advance ...
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bioRxiv - Immunology 2022Quote: ... T cell subsets before injection and recovered at day 8 post-HCT were frozen in Trizol (Thermo Fisher Scientific) and conserved at −80°C until analysis.
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bioRxiv - Systems Biology 2022Quote: ... The protein fractions were quantified using an 8 point Pierce BCA Protein Assay Kit (Thermo Fisher Scientific, Cat.# PI23225), and 20 µg of protein per sample diluted to 35µL using NuPAGE LDS sample buffer (1x final conc. ...
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bioRxiv - Plant Biology 2023Quote: ... Yields (8-21 μg at 350-900 ng/μL) were determined using the Qubit fluorometer (ThermoFisher, Waltham, MA, USA). Fragments of >40 Kbp were collected for library preparation using BluePippin pulsed-field electrophoresis (Sage Science ...
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bioRxiv - Molecular Biology 2022Quote: ... For the RNase T1 ladder 0.2 pmol of labeled sRNA was incubated with 8 μL of 1x sequencing buffer (Ambion) at 95 °C for 1 min followed by the addition of 1 μL RNase T1 (0.1 U/ μl ...
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bioRxiv - Molecular Biology 2022Quote: RNA was isolated from samples collected within 8 days of symptoms onset using either KingFisher Flex System (ThermoFisher Scientific) or Extracta 96 (Loccus ...
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bioRxiv - Physiology 2024Quote: ... maintained at 8°C and coupled to an Orbitrap ELITE™ Hybrid Ion Trap-Orbitrap Mass Spectrometer (ThermoFisher Scientific). 5 µL of extract were injected into an Agilent Poroshell 120 EC-C18 column (2.1 x 100 mm ...
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bioRxiv - Plant Biology 2024Quote: ... in each condition total RNA was extracted from a pool of 8 leaves and extracted using TRIzol reagent (Invitrogen). Three independent biological replicates were analysed ...
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bioRxiv - Bioengineering 2024Quote: ... and treated with purified viral particles resuspended in 0.8 mL DMEM containing 8 µg/mL polybrene (Thermo Fisher Scientific). The cells were spinfected by centrifuging the six-well plates at 1050 x g for 90 min at 30 °C and the returned to the 37 °C incubator for another 48 h ...
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bioRxiv - Neuroscience 2023Quote: Colons and small intestines (without caecum) from female mice (8-20wks) were isolated and transferred into RPMI-1640 (Gibco) medium supplemented with L-glutamine ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were washed 8-10 times in PBS for 5 minutes and incubated with DAPI (0.1 mg/ml in PBS; #62248, Invitrogen) to stain nuclei ...
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bioRxiv - Microbiology 2024Quote: ... HCT-8 cells were cultured in maintenance medium RPMI 1640 with GlutaMAX™ and HEPES supplements (ThermoFisher Scientific # 72400047) plus 5% foetal calf serum (FCS ...
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bioRxiv - Molecular Biology 2024Quote: Circular mtDNA was first linearized by adding 12.5 U/μL MssI enzyme (Pmel, 8-bp endonuclease) (ThermoFisher, MA, USA) for 2 h at 37 °C in accordance with the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... were transfected into HepaCam enriched astrocytes seeded in 8-well chamber slides using the lipofectamine LTX reagent (0.6μl) and plus reagent (0.2μl, Thermo Fisher Scientific, A1262) containing 40μl Opti-MEM following the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2024Quote: ... 50 μL reactions were prepared with 8 nM SWR1C mixed with 0.5 μM phosphate sensor (fluorophore-conjugated phosphate binding protein, ThermoFisher) in remodeling buffer ...
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bioRxiv - Cancer Biology 2024Quote: Frozen samples (n=8) were OCT embedded and sectioned at 10μm on a Cryostar NX70 cryostat (Thermo Fisher Scientific). Capture sections were fixed with methanol ...
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bioRxiv - Cell Biology 2024Quote: ... Nuclei were pelleted at 800g for 10 min at 4°C and washed twice in buffer 1 and once in freezing buffer (1M Tris pH 8, 100% glycerol, 100mM MgAc2, 0.5M EDTA, 1M DTT, 1x PTase inhibitor cocktail Roche, 20U/µl SUPERase In Ambion). Nuclei were resuspended in freezing buffer ...
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bioRxiv - Neuroscience 2023Quote: ... at DIV 8 the astrocytes were transfected with the expression plasmids using Lipofectamine LTX with Plus Reagent (Thermo Scientific) per the manufacturer’s protocol.
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bioRxiv - Cell Biology 2023Quote: ... IMCD3 cells were seeded in 8-well chamber slides (Nunc™ Lab Tek™ II Chamber Slide™ System) followed by siRNA treatment the next day with either the control siRNA or Adcy3 ...
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bioRxiv - Cancer Biology 2023Quote: Corning costar plates (#3422 and #3428) with polycarbonate membrane and 8 micron pore size were purchased from Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: Blood was collected from hearts of freshly sacrificed mice to tubes coated with 0.5 M EDTA pH 8 (Invitrogen) to prevent clotting ...
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bioRxiv - Immunology 2023Quote: ... and finally glued with picodent twinsil extrahart (Picodent) to the bottom of 8- or 16-well LabTek chambers (Nunc), from which the original glass bottom had been removed ...
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bioRxiv - Microbiology 2023Quote: ... and loaded to gels (8 μL/well) for SDS-PAGE and Western blotting (NuPage Novex system, Thermo Fisher Scientific) to 0.45 μm nitrocellulose membranes (BioRad laboratories).
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bioRxiv - Genomics 2023Quote: ... A second reaction mixture (8 μL/reaction) containing the reaction buffer (5µL of First strand Buffer 5X, Invitrogen, France), DTT (Dithiothreitol ...
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bioRxiv - Immunology 2023Quote: ... Biopsies were first transferred in the dissociation medium consisting of 10 ml HBSS (free of calcium and magnesium) containing EDTA (0.5M, pH 8, Invitrogen) and HEPES (1M ...