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Citations for Thermo Fisher :
2751 - 2800 of 10000+ citations for 6 1 4 Dioxa 8 azaspiro 4.5 dec 8 yl 3 pyridinyl boronic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... 8 x 106 HEK293T cells were plated in 10-cm tissue culture dishes and transfected using Lipofectamine2000 (Invitrogen) with plasmids encoding different CoV spike proteins or VSV-G protein ...
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bioRxiv - Cancer Biology 2020Quote: ES cell-derived haemogenic endothelium cultured on gelatine-coated 8-well Lab-Tek II Chamber Slide System (ThermoFisher) was fixed using 4% PFA for 10 min ...
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bioRxiv - Biochemistry 2022Quote: ... were maintained at 37 °C in a humidified atmosphere of 8% CO2 and cultured in high-glucose Dulbecco’s modified Eagle’s medium (Gibco) supplemented with 10% (v/v ...
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bioRxiv - Cell Biology 2022Quote: ... 8 × 106 cells were seeded in a 15-cm plate with 45 mL of DMEM for SILAC (ThermoFisher) supplemented with foetal calf serum (10% v/v) ...
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bioRxiv - Microbiology 2021Quote: ... were washed twice with 300 μL 1X TBS-T (2.4 g Tris-Base, 8 g NaCl, 0.1 % Tween20) using a magnetic stand (ThermoFisher) before coupling them with 200 μl of B domains in TBS at 0.125 ng/ml ...
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bioRxiv - Cancer Biology 2021Quote: ... the indicated cells were transfected with LentiCRISPR-V2 plasmid carrying sgARID1A (Supplementary Table 8) using Lipofectamine 2000 (Invitrogen), and further selected by 1 μg/mL puromycin (Selleck ...
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bioRxiv - Developmental Biology 2022Quote: ... minced and processed into a single cell suspension in a Collagenase I (8 mg/5ml, 17100017, Life Technologies) /Dispase (1:10 ...
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bioRxiv - Bioengineering 2020Quote: Day 8 blastocysts were labelled and imaged within devices using the ratiometric mitochondrial dye JC-10 (Molecular Probes). Briefly ...
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bioRxiv - Molecular Biology 2021Quote: ... Equal amounts of proteins were resolved on 8% Bis-Tris page gel (Invitrogen, Thermo Scientific, Waltham, MA, USA). Proteins were resolved eletro-trasferred onto polyvinylidene fluoride (PVDF ...
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bioRxiv - Molecular Biology 2021Quote: ... Equal amounts of proteins were resolved on 8% Bis-Tris page gel (Invitrogen, Thermo Scientific, Waltham, MA, USA). Proteins were resolved eletro-trasferred onto polyvinylidene fluoride (PVDF ...
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bioRxiv - Molecular Biology 2021Quote: ... The droplets were injected with a reverse transcription mix constituted of 25 mM Tris-HCl pH 8 (Invitrogen), 8 mM DTT (Invitrogen) ...
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bioRxiv - Bioengineering 2020Quote: ... or P[8] under control of the AOX1 promoter using a commercial vector (pPICZ A, Thermo Fisher Scientific). Modified vectors containing markers for selection on G418 (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2019Quote: ... Mixtures of 20-μl of the sample (8-10 μM final, considering hexamers) and SYPRO Orange (× 100; Invitrogen) in Sol C were subjected to a temperature gradient from 20 to 100 °C with increments of 0.3 °C every 10 sec ...
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bioRxiv - Molecular Biology 2021Quote: ... was added into the 48S IC and 80S IC mixtures in reverse-transcription buffer (0.5 mM dNTPs, 8 mM MgCl2, 0.15 U/μL SuperScript III reverse transcriptase (Invitrogen)) and the reaction was incubated for 45 min at 37 °C ...
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bioRxiv - Immunology 2020Quote: ... for 2.5 s with a blot force of 8 using a Vitrobot Mark IV (Thermo Fisher, Waltham, MA) set to 4°C with 100% humidity ...
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bioRxiv - Cancer Biology 2020Quote: 3D acini were set up as described above in either 8-well coverglass chamber slides (Nunc, LabTek-II) or in 96 well plates (Greiner ...
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bioRxiv - Genetics 2021Quote: ... PCR products were directly run on an 8% PAGE gel and visualized with SYBR Gold (ThermoFisher S-11494), or subject to AMPure XP beads (Beckman Coulter A63881 ...
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bioRxiv - Immunology 2020Quote: ... were coated at room temperature for 8 hours with 1ug/mL PolyRab anti-His antibody (ThermoFisher, PA1-983B), followed by overnight blocking with blocking buffer containing 1x PBS ...
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bioRxiv - Immunology 2020Quote: ... were coated at room temperature for 8 hours with 1ug/mL PolyRab anti-His antibody (ThermoFisher, PA1-983B), followed by overnight blocking with blocking buffer containing 1x PBS ...
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bioRxiv - Immunology 2020Quote: ... were coated at room temperature for 8 hours with 1ug/mL PolyRab anti-His antibody (ThermoFisher, PA1-983B), followed by overnight blocking with blocking buffer containing 1x PBS ...
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bioRxiv - Immunology 2021Quote: ... Virus was titrated by adding serial dilutions of virus supernatant (8 replicates) on VeroE6 cells in DMEM (Gibco) containing 2% FBS ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells at 5000 or 10000/cm2 were cultured on an 8-well chamber slide (Thermo Scientific, cat# 177445) or in a 6-well plate coated with 1 mg/ml Matrigel (Corning ...
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bioRxiv - Immunology 2020Quote: ... were coated at room temperature for 8 hours with 1ug/mL PolyRab anti-His antibody (ThermoFisher, PA1-983B), followed by overnight blocking with blocking buffer containing 1x PBS ...
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bioRxiv - Immunology 2020Quote: ... were coated at room temperature for 8 hours with 1ug/mL PolyRab anti-His antibody (ThermoFisher, PA1-983B), followed by overnight blocking with blocking buffer containing 1x PBS ...
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bioRxiv - Genetics 2019Quote: ... A dye stock was prepared containing 0.3 mM Fluo-8 AM and 3.3% Pluronic F-127 (ThermoFisher Scientific) and mixed thoroughly ...
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bioRxiv - Biochemistry 2022Quote: ... and HCT116 cells were transfected with 8 µg each of appropriate constructs using Lipofectamine 2000 (Thermo Fisher Scientific). Approximately 24 hours post-transfection ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were centrifuged again at 500g for 8 min and resuspended in PBS (Gibco, cat. no. 14190-094) with 0.04% BSA (Sigma-Aldrich ...
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bioRxiv - Genetics 2022Quote: ... On the day of transfection 8 μg of plasmid DNA was diluted in 125 μl Opti-MEM (Thermofisher) and 25 μl of FuGENE Reagent was diluted with 100 μl Opti-MEM ...
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bioRxiv - Immunology 2022Quote: ... RAW:iNos-eGFP cells were plated in a 8-well Lab-Tek II chambered cover-glass system plate (Nunc) at a density of 100,000 cells/mL in 500 µL (50,000 cells/well ...
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bioRxiv - Microbiology 2022Quote: ... a pixel size of 4.31 Å and a defocus of −8 µm using Tomography 5.7 software (Thermo Fisher) (Table S2) ...
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bioRxiv - Plant Biology 2022Quote: ... RLP30 and SOBIR1 coding sequences were first cloned into pCR™8/GW/TOPO™ (Thermo Fisher Scientific) and subsequently fused via Gateway cloning to a C-terminal YFP (pB7YWG230) ...
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bioRxiv - Biochemistry 2022Quote: 8-well chamber slides were coated with 25 μg/ml DQ collagen type I (Invitrogen, catalog no. D12060) containing a 1:11 ratio of DQ-conjugated to non-conjugated bovine collagen I for 2 h at room temperature and seeded with parental RPE-1 or D12 cells followed by culture for 24 hours in serum-free DMEM+F12 culture medium ...
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bioRxiv - Neuroscience 2022Quote: ... iPSCs were maintained on Matrigel-coated plates in Essential 8 medium (E8; Thermo Fisher Scientific Ltd., Vantaa, Finland). For differentiation ...
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bioRxiv - Molecular Biology 2022Quote: ... Approximately 1,000 MLO-Y4 cells were seeded onto collagen-I coated 8-well chambers (NUNC™, Rochester, NY) and cultured as described previously60 ...
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bioRxiv - Cell Biology 2022Quote: H2O2 quantification was performed on triplicates of 8 adult males using Amplex Red Hydrogen Peroxide assay kit (Invitrogen) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... the supernatant was collected in a 50 mL tube containing 8 mL of fetal bovine serum (FBS) (Gibco) on ice ...
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bioRxiv - Immunology 2024Quote: ... the femoral and tibial bones of 8-10-week-old mice were flushed with RPMI media (Thermo Fisher) and the bone marrow suspension was passed through a 70 mm cell strainer ...
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bioRxiv - Molecular Biology 2024Quote: ... After the PCR reaction the amplicons were cloned into the pCR™8/GW/TOPO vector (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... Equal amounts of protein were separated on 8% SDS-PAGE and then transferred onto nitrocellulose membranes (Thermo Fisher). After blocking in 5% milk/TBST at room temperature for 1 h ...
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bioRxiv - Cell Biology 2023Quote: We assembled the patterned rectangular coverslip onto 8-multiwell chambers (Nunc® Lab-Tek® Chamber SlideTM system) after removing pre-mounted slide glasses on their bottom ...
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bioRxiv - Neuroscience 2023Quote: SH-SY5Y (10.000 cells/well) were seeded onto 8 well-chamber microscope slides (Nunc Lab Tech, ThermoFisher Scientific) and infected with the SARS-CoV-2 B.1 strain (MOI 0.1 ...
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bioRxiv - Neuroscience 2023Quote: SH-SY5Y (10.000 cells/well) were seeded onto 8 well-chamber microscope slides (Nunc Lab Tech, ThermoFisher Scientific) and infected with the SARS-CoV-2 B.1 strain (MOI 0.1 ...
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bioRxiv - Cell Biology 2023Quote: ... and propagated under feeder-free conditions using Essential 8 Medium on a Geltrex-coated plate (ThermoFisher, Waltham, MA).
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bioRxiv - Cell Biology 2023Quote: Primary human parenchymal lung fibroblasts from non-COPD and COPD patients (n=8) were grown in DMEM (Gibco) supplemented with 5% FBS and 2% antibiotics at 37°C and 5% CO2 ...
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bioRxiv - Cancer Biology 2023Quote: ... resolved for about 1cm (80 volts; 63 mA; 8 watts) before being stained with Imperial protein stain (ThermoFisher). After de-staining to remove the background ...
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bioRxiv - Genetics 2023Quote: ... and72°C for 8 min for the final extension using a thermal cycler (GeneAmp 9700, Applied Biosystems, USA). The final PCR product was separated on 3% agarose gel containing 0.5μgethidium bromide in 1X TBE buffer and gel images were documented in the gel documentation system (Gene Genius ...
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bioRxiv - Molecular Biology 2023Quote: ... Between 8-15 μg of DNA was incubated with 100μl (∼10 mg) of Dynabeads MyOne Streptavidin C1 (Invitrogen) for 15 min with rotation ...
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bioRxiv - Microbiology 2023Quote: ... were cultured at 37°C with 8% CO2 while shaking at 130 rpm in ExpiCHO expression media (GIBCO). Vero E6-TMPRSS2 cells that stably express human TMPRSS2 (NIBIOHN ...
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bioRxiv - Bioengineering 2023Quote: Cell culture medium Essential 8 (E8) Flex Medium and recombinant human vitronectin (rhVTN-N) were purchased from Thermofisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... supplemented with 8% FBS (HyClone) and 500 µg/ml gentamicin (Quality Biologicals) and 500 µg/ml geneticin (Gibco) at 37°C and 5% CO2 ...