Labshake search
Citations for Thermo Fisher :
251 - 300 of 4963 citations for Tryptase delta Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2022Quote: ... The peptide concentration was measured using the Pierce™ Quantitative Colorimetric Peptide Assay (Thermo Scientific cat. # 23275). The digested peptides were subjected to simple C18 clean up using a C18 disk plug (3M Empore C18 ...
-
bioRxiv - Neuroscience 2023Quote: ... desalted peptides was separated into 9 fractions with the high pH reversed-phase peptide fractionation kit (ThermoFisher). Fractions with increasing percentages of acetonitrile (ACN ...
-
bioRxiv - Microbiology 2023Quote: ... the concentration of peptides was measured using Pierce Quantitative fluorometric peptide assay (ThermoFisher Scientific, Rockford, IL, USA).The resulting peptides were sequenced on a Q-Exactive Hybrid Quadrupole-Orbitrap Mass Spectrometer (ThermoFisher Scientific ...
-
bioRxiv - Cancer Biology 2023Quote: ... The peptide concentration in the supernatant was quantified using a quantitative colorimetric peptide assay from Thermo Fisher Scientific (Cat ...
-
bioRxiv - Pathology 2024Quote: ... The concentration of the peptides was determined using Quantitative Colorimetric Peptide Assay (Thermo Fisher Scientific, Cat#23275). Tandem mass tag (TMT)-based labeling of peptides was performed using the TMT10plex™ Isobaric Label Reagent Set (Thermo Fisher Scientific ...
-
bioRxiv - Cell Biology 2023Quote: ... and the supernatant was recovered for peptide desalting using Pierce™ Peptide Desalting Spin Column (ThermoFisher: 89851) as described by the manufacturer ...
-
bioRxiv - Microbiology 2024Quote: ... 0.1% TFA and peptide content was estimated with a Pierce™ Quantitative Colorimetric Peptide Assay (Thermo Scientific) according to the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2021Quote: ... were used following biotin blocking of tissues samples using endogenous Biotin-blocking kit (Life Technologies Corporation/Thermo fisher, E21390). For Aza-peptide preparation ...
-
bioRxiv - Molecular Biology 2021Quote: ... were used following biotin blocking of tissues samples using endogenous Biotin-blocking kit (Life Technologies Corporation/Thermo fisher, E21390). For Aza-peptide preparation ...
-
bioRxiv - Cell Biology 2020Quote: ... and 1X PBS) before blocking for one hour with blocking buffer (Bovine Serum Albumin (Fisher Scientific, Fair Lawn, NJ), Normal Donkey Serum (Jackson ImmunoResearch Inc. ...
-
bioRxiv - Physiology 2022Quote: ... Non-specific antigens were blocked for 60 min using a blocking buffer (Sea Block Blocking Buffer; ThermoFisher Scientific, USA). Then ...
-
bioRxiv - Molecular Biology 2021Quote: The peptide concentration was determined by using the Pierce Quantitative Colorimetric Peptide Assay (Thermo Fisher Scientific, Rockford, IL) and 180 µg peptides from each sample were loaded onto MacroSpin column (NestGroup ...
-
bioRxiv - Immunology 2019Quote: ... containing LCMV peptides (1:1000, Peptide 2.0 Inc.) and Protein Transport Inhibitor Cocktail (1:500, Thermo Fisher Scientific). Cells were incubated for 4 hours at 37°C and then surface antigens were stained as described above ...
-
bioRxiv - Microbiology 2021Quote: ... Labeled peptides of all samples were mixed equally and desalted by peptide desalting spin columns (Thermo Fisher, 89,852). A C18 column (Waters BEH C18 ...
-
bioRxiv - Plant Biology 2022Quote: LC-MS/MS analysis of peptides and phosphopeptides – Peptides mixtures were analyzed by nanoflow Easy-nLC (Thermo Scientific) and Orbitrap hybrid mass spectrometer (Q-exactive HF ...
-
bioRxiv - Systems Biology 2022Quote: ... Peptide concentrations were determined using a fluorimetric peptide assay kit following the manufacturer’s instructions (Thermo Scientific, Cat#23290).
-
bioRxiv - Plant Biology 2019Quote: ... followed by peptide fractionation using the Pierce High pH Reverse-Phase Peptide Fractionation Kit (Thermo Scientific, Rockford, IL). Eight fractions of peptides from each sample were subjected to liquid chromatography-tandem mass spectrometry (LC-MS/MS ...
-
bioRxiv - Cell Biology 2021Quote: ... Phospho-peptide enrichment was then performed using the High-SelectTM TiO2 Phospho-peptide Enrichment kit (Thermo Fisher Scientific). Each replicate was divided between two TiO2 columns and prepared according to the manufacturer’s instructions ...
-
bioRxiv - Developmental Biology 2022Quote: ... Peptides were then desalted by reverse-phase chromatography using Pierce peptide desalting spin columns (Thermo Fisher part #89852). Final resuspension was done with Solvent A (0.1% formic acid in Fisher Optima LC/MS grade water ...
-
bioRxiv - Plant Biology 2022Quote: ... Peptides were subsequently fractionated using high pH reversed-phase peptide fractionation kit (Thermo Fisher Scientific, San Jose, USA) and pooled into 8 fractions [43] ...
-
bioRxiv - Systems Biology 2023Quote: Phospho-peptide enrichment was carried out using the High-Select TiO2 Phospho-peptide Enrichment kit (Thermo Fisher, A32993), as per manufacturer’s instructions ...
-
bioRxiv - Biophysics 2023Quote: ... The concentration of peptide was determined using Pierce quantitative fluorometric peptide assay (Cat# 23290, Thermo Scientific, Rockford, IL). 11 – 18 μL of the peptides were analyzed through nano-ESI-LC/MS/MS using the Orbitrap Exploris mass spectrometer coupled to a Dionex nano-LC system (Thermo Scientific ...
-
bioRxiv - Systems Biology 2023Quote: ... The peptide concentration of this pool was determined using a fluorimetric peptide assay kit (Thermo Scientific, Cat#23290). Peptide concentrations per sample were estimated by multiplying the optical density recorded at harvest with the ratio between pool peptide concentration and the median at-harvest optical density.
-
bioRxiv - Molecular Biology 2023Quote: ... The peptide mix was then fractionated using the Pierce High pH Reversed-Phase Peptide Fractionation Kit (ThermoFisher Scientific). The 8 obtained fractions were analyzed by online nanoliquid chromatography coupled to MS/MS (Ultimate 3000 RSLCnano and Q-Exactive HF ...
-
bioRxiv - Cancer Biology 2023Quote: The peptide amount in each sample was estimated using a quantitative colorimetric peptide assay kit (Thermo Fisher Scientific). Within each batch ...
-
bioRxiv - Cell Biology 2024Quote: ... soluble peptide suspension was transferred into fresh Eppendorf tubes and peptide concentration was determined by microBCA (Thermo Scientific). Equal amounts of peptides for each sample (150-200 µg ...
-
bioRxiv - Genetics 2024Quote: ... The concentration of tryptic peptides was measured using the Pierce Quantitative Peptide Assays kit (ThermoFisher Scientific cat# 23275), and 25 µg of peptides were separated on a home-made reverse-phase C18 column in a pipet tip ...
-
bioRxiv - Microbiology 2023Quote: ... of 78 of 15-mer peptides covering DBV GnH formulated at 0.625μg/ml for each peptide in RPMI medium (Gibco) in the 96-well plate ...
-
bioRxiv - Microbiology 2020Quote: ... Blocking was carried out overnight at 4°C in a humidified dark chamber using Blocking Aid Solution (Thermo Fisher Scientific). Primary antibody diluted in Blocking Aid Solution (Anti-PvGAMA 1:1200 ...
-
bioRxiv - Physiology 2019Quote: ... Endogenous peroxidase activity was blocked with 3% hydrogen peroxide in phosphate-buffered saline (PBS) followed by an additional blocking step with 1% Tyramide Signal Amplification (TSA) blocking reagent (TSA kit, T20935, Invitrogen) and Mouse-on-Mouse blocking reagent (Vector Laboratories ...
-
bioRxiv - Neuroscience 2021Quote: ... the FUS variants (WT, Ala sub, PM, and delta 15) were synthesized and ligated into pcDNA3.1(+) Hygro by GeneArt (ThermoFisher Scientific). These constructs were then digested at NheI/HindIII sites upstream of the FUS sequence ...
-
bioRxiv - Biochemistry 2020Quote: Growth experiments of selected strains carrying FDH variants were performed by inoculating M9 medium with the respective strain at a starting OD600 of 0.01 in 96-well microtiter plates (Nunclon Delta Surface, Thermo Scientific) and carried out at 37 °C ...
-
bioRxiv - Physiology 2020Quote: ... coupled to an Isotopic Ratio Mass Spectrometer (IRMS Delta V Plus with a Conflo IV Interface, Thermo scientific, Bremen, Germany). Atom% of samples was derived from isotope ratio data and calculated using the Vienna Pee Dee Belemnite standard (RVPDB = 0.0112372)
-
bioRxiv - Synthetic Biology 2021Quote: ... Cultures were inoculated into the M9 media to an OD600 of 0.01 in a 96-well microtiter plate (Nunclon Delta Surface, Thermo Scientific). Each well contained 150 μL of culture and 50 μL mineral oil (Sigma-Aldrich ...
-
bioRxiv - Bioengineering 2022Quote: ... and hCoV-19/South Korea/KDCA2950/2021 (Delta variant) were propagated in Vero E6 cells grown in DMEM high glucose media (Gibco) supplemented with 10% FBS and 1% antibiotic-antimycotic in 37°C ...
-
bioRxiv - Systems Biology 2022Quote: ... Washed cells were used to inoculate growth experiments to an optical density (OD600) of 0.01 in 96-well microtiter plates (Nunclon Delta Surface, Thermo Scientific) at 37°C ...
-
bioRxiv - Microbiology 2021Quote: ... Cells were then inoculated into the various test media to OD600 of 0.01 and distributed into 96-well microtiter plates (Nunclon Delta Surface, Thermo Scientific). Each well contained 150 μL of culture and 50 μL mineral oil (Sigma-Aldrich ...
-
bioRxiv - Microbiology 2022Quote: ... Stable carbon isotope signatures of CH4 were analyzed by isotope-ratio mass spectrometry (Delta V plus, Thermo Scientific, Dreieich, Germany) coupled to a GC-Isolink / Trace-GC (Thermo Scientific ...
-
bioRxiv - Microbiology 2022Quote: ... albicans using broth microdilution assays carried out in 96-well microtiter plates (Nunclon Delta, Thermo Fisher Scientific, Waltham, MA, USA). The assay was performed with 1×104 cells/mL and 1×106 cells/mL in 0.05×PDB ...
-
bioRxiv - Synthetic Biology 2022Quote: ... Growth experiments were inoculated with the washed cells to an OD600 of 0.01 in 96-well microtiter plates (Nunclon Delta Surface, Thermo Scientific) at 37 °C ...
-
bioRxiv - Neuroscience 2022Quote: Viral fragments were obtained either by RT-PCR on RNA extracted from Vero-E6 cells infected by Delta using the SuperScript IV VILO Master Mix (#11756050, Thermofisher) according to the manufacturer protocol ...
-
bioRxiv - Microbiology 2022Quote: ... The δ13CDIC values in pore water were analyzed by a multiflow-isotope ratio mass spectrometer (Thermofisher, Delta V Advantage, USA), with an analytical error of ±0.2‰ ...
-
bioRxiv - Microbiology 2022Quote: ... and 100 μL were dispensed in each well of a U-shaped bottom 96-well microplate (Nunclon Delta-Treated, U-Shaped-Bottom Microplate, Nunc). Antibiotics ...
-
bioRxiv - Microbiology 2023Quote: ... were transfected with each plasmid expressing SPΔC of SARS-CoV-2 Delta variant (1.2 µg DNA) or human influenza A virus M2 (5 µg DNA) using Lipofectamine 3000 (Invitrogen, USA) to produce 293TN-S target cells ...
-
bioRxiv - Cancer Biology 2023Quote: ... PDO was cultured in Nunclon™ Delta Surface (Thermoscientific) on Geltrex dome (Reduced growth factor basement membrane, hESV qualified, Gibco) suspended in PaTOM growth media (Huang et al. ...
-
bioRxiv - Biochemistry 2023Quote: ... by transient transfection of pLV-EF1a-Prom1-Strep with VSVG and Delta 8.9 plasmids (a generous gift from K. Hochedlinger, Massachusetts General Hospital) using the Lipofectamine 3000 system (Thermo Fisher) and incubated overnight at 37 °C ...
-
bioRxiv - Biochemistry 2024Quote: ... fowleri trophozoites were grown to near 100% confluency in 6-well TC-treated plates (Thermo Scientific Nunclon Delta Surface 140685). HEX (25 µM ...
-
bioRxiv - Cell Biology 2019Quote: ... or HA peptide (Thermo Fisher Scientific, 26184).
-
bioRxiv - Microbiology 2019Quote: ... Peptides were labelled with TMT6plex (ThermoFisher Scientific), desalted with solid-phase extraction on a Waters OASIS HLB µElution Plate (30 µm) ...
-
bioRxiv - Cancer Biology 2020Quote: ... Eluted peptides were analyzed by Thermo Scientific Q Exactive or Q Exactive plus mass spectrometer utilizing a top 15 methodology in which the 15 most intense peptide precursor ions were subjected to fragmentation ...