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Citations for Thermo Fisher :
451 - 500 of 4963 citations for Tryptase delta Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... cells were rinsed again thrice with 1×PBS before an overnight blocking stage at 4°C in 1×SEA BLOCK blocking buffer (Thermo Fisher Scientific, Poland).
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bioRxiv - Developmental Biology 2021Quote: Phospho-peptides were fractionated using alkaline reversed phase chromatography (Pierce™ High pH Reversed Phase Peptide Fractionation Kit, Thermo Scientific #84868) into 12 fractions using increasing concentrations of acetonitrile from 10%-80% in basic binding buffer ...
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bioRxiv - Molecular Biology 2022Quote: ... All ten vials of samples were combined and approximately 100 μg peptide digests from this mix were fractionated using Pierce High pH Reversed-Phase Peptide Fractionation Kit (ThermoFisher Scientific) according to instructions from manufacturer ...
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bioRxiv - Biochemistry 2020Quote: ... samples were briefly centrifuged at 13,000 g and peptide-containing supernatant was used for further analysis Peptides were fractionated into eight fractions using High-pH Reversed-Phase Peptide Fractionation Kit (Thermo Fisher). Peptide fractions were dried in SpeedVac (Eppendorf ...
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bioRxiv - Bioengineering 2021Quote: ... then incubated with 50 µL of 1 µM biotinylated peptide in peptide binding buffer (1% sheared salmon sperm DNA (Invitrogen, AM9680), 10 mM HEPES ...
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bioRxiv - Cancer Biology 2021Quote: ... and remaining ~1.1 mg of multiplexed peptides were used for phospho-peptide enrichment by High-Select Fe-NTA Phosphopeptide enrichment kit (Thermo Fisher) after manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... The molecular mass of purified peptide was determined by 18% SDS-PAGE and the peptide concentration was obtained by Bicinchoninic protein assay kit (Thermo Scientific).
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bioRxiv - Cell Biology 2022Quote: ... TMT-labeled peptides were pre-fractionated with the basic pH reversed-phase C18 peptide fractionation kit following manufacturer’s manual (Thermo Fisher, 84868). Typically ...
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bioRxiv - Biochemistry 2019Quote: ... peptide concentration and primary amine reactivity were quantified using the Pierce quantitative fluorometric peptide assay (Thermo Fisher Scientific; cat. no. 23290) and Pierce quantitative colorimetric peptide assay (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2019Quote: ... were raised in rabbits by immunization with keyhole limpet hemocyanin-conjugated peptides and affinity-purified by columns conjugated with the peptides (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2022Quote: The phosphopeptide enrichment step flow through and washes were dried down and approximately 120 µg peptides were fractionated using the TMT fractionation protocol of Pierce high pH basic reversed-phase peptide fractionation kit (Thermo Fisher Scientific™ Cat no 84858 ...
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bioRxiv - Biochemistry 2023Quote: The RC9 peptides with the sequence GGKGMGFGL was prepared using solid-state peptide synthesis with Fluorenylmethyloxycarbonyl (FMOC)-based chemistry by Life Technologies, Inc and purified by reversed-phase high pressure liquid chromatography (HPLC ...
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bioRxiv - Cell Biology 2023Quote: ... 50 mM Tris pH 7.5) followed by elution in 100 µl TBS + 150 ng/µL competitor peptide (Pierce 3x DYKDDDDK Peptide, Thermo Fisher Scientific) at 37 °C for 30 min.
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bioRxiv - Molecular Biology 2023Quote: ... 300 µL (90 µg) of reconstituted mixture of labeled peptides was fractionated using Pierce High pH Reversed-Phase Peptide Fractionation kit (84868, Thermo Fisher) according to the manufacturer’s protocol for TMT-labeled peptides ...
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bioRxiv - Cell Biology 2023Quote: ... Dried peptides were resuspended in DI water and then quantified using Pierce Quantitative Colorimetric Peptide Assay (Thermo Fisher Scientific, Rockford, USA).
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bioRxiv - Plant Biology 2024Quote: ... Labeled peptides were separated into 6 fractions with Pierce™ High pH Reversed-Phase Peptide Fractionation Kit (Thermofisher, Cat. no. 84868) and dried in a Speed Vac at 45 °C for 3.5 hours ...
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bioRxiv - Cell Biology 2024Quote: ... The deuterium exchange levels were determined by subtracting the centroid mass of undeuterated peptide from the centroid mass of deuterated peptide using HDExaminer (Version PD1.4, Thermo-Fisher Scientific, USA).
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bioRxiv - Plant Biology 2019Quote: ... Phospho-peptides were enriched from the remaining sample (900 µg) using High-Select Fe-NTA Phospho-peptide Enrichment Kit (A32992, Thermo Fisher Scientific). The TMT labeled samples were analyzed on a Fusion Lumos mass spectrometer (Thermo Fisher Scientific) ...
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bioRxiv - Systems Biology 2019Quote: ... Lymphocyte samples were prepared as 50 ng/µL total sample peptide concentration with Pierce Peptide Retention Time Calibration Mixture (PRTC, Thermo Fisher Scientific) spiked in at a final concentration of 2 fmol/µL and queued in blocked and randomized order with two technical replicates analyzed per sample ...
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bioRxiv - Biochemistry 2022Quote: ... peptides were fractionated into a final eight fractions using a Pierce High pH reversed-Phase Peptide Fractionation Kit (Thermo Fisher Scientific, 84868), according to the manufacturer’s instructions.
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bioRxiv - Genomics 2022Quote: ... A portion of the peptides from the samples were pooled and fractionated using a Pierce High pH Reversed-Phase (HPRP) Peptide Fractionation Kit (Thermo Fisher Scientific) to generate a spectral library ...
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bioRxiv - Microbiology 2022Quote: Peptide concentrations were measured for fraction two of each sample using the Quantitative Fluorometric Peptide Assay kit (Pierce™ Thermo Fisher Scientific) according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2020Quote: ... An aliquot of the tryptic digests was used to determine the concentration of the peptide mixtures by using Pierce™ Quantitative Colorimetric Peptide Assay (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... The flow-through containing endogenous peptides was collected and peptide concentration was measured using a bicinchoninic acid (BCA) protein assay (Thermo Scientific Pierce) as per manufacturer’s protocol ...
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bioRxiv - Bioengineering 2022Quote: ... The rat tail collagen I protein and GFOGER peptide peptide were subsequently blocked using methyl-PEG24-amine (MA-PEG24) (Thermo Fisher Scientific). Post functionalization ...
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bioRxiv - Neuroscience 2023Quote: ... The combined isobaric-labeled peptide solution was fractionated into eight fractions per manufacturer’s instructions using high pH reversed-phase peptide fractionation columns (ThermoFisher Scientific, Cat# PI84868). All eight peptide solutions were dried in a speed vac ...
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bioRxiv - Biochemistry 2023Quote: ... Mixed peptide samples were prepared by 9ul per sample and separated using the High pH Reversed-Phase Peptide Isolation Kit (Thermo Fisher Scientific) as directed ...
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bioRxiv - Neuroscience 2023Quote: ... The fractions were dissolved with 0.1% formic acid and peptide concentration was determined with Quantitative Colorimetric Peptide Assay (ThermoFisher Scientific, cat# 23275).
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bioRxiv - Cell Biology 2023Quote: ... Those peptides were divided into several fractions using the Pierce High pH Reversed-Phase Peptide Fractionation kit (Thermo Fisher Scientific, Waltham, MA) according to the manufacturer protocol ...
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bioRxiv - Immunology 2023Quote: ... The combined flowthrough and washes were dried down in a speed vac to completion for subsequent bench-top fractionation of non-phospho peptides by alkaline reversed phase chromatography (PierceTM High pH Reversed Phase Peptide Fractionation Kit, Thermo Scientific #84868) with a modified elution scheme as described in81 and MS analysis.
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bioRxiv - Neuroscience 2023Quote: ... The TMT labeled peptide pools were separated into eight fractions with the Pierce™ High pH Reversed-Phase Peptide Fractionation Kit (ThermoFisher Scientific), evaporated ...
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bioRxiv - Molecular Biology 2024Quote: ... Pooled TMT-labelled peptides were cleaned and fractionated using the Pierce High pH Reversed-Phase Peptide Fractionation Kit (Thermo Fisher Scientific, 84868) according to manufacturer’s instruction for TMT experiments ...
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bioRxiv - Neuroscience 2021Quote: The CDS of NeuroD1 and 3’UTRs of Delta and Notch was cloned using sea urchin cDNA into Zeroblunt vector (ThermoFisher Scientific, Waltham, MA) (Table 1) ...
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bioRxiv - Biochemistry 2019Quote: ... 50 μL of the supernatant (see the previous section) from each sample was transferred to a black 96-well Nunclon Delta Surface microplate (Thermo Scientific Nunc, Roskilde, Denmark) and incubated with the catalyst (1× ...
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bioRxiv - Microbiology 2019Quote: ... and the carbon isotope composition was measured via GC-IRMS on a Thermo Finnigan Trace GC connected with a DELTA V Plus IRMS system via GC Isolink (Thermo Scientific, Bremen Germany). The detailed chromatographic and mass spectrometric parameters were described by Kellermann et al ...
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bioRxiv - Microbiology 2019Quote: The δ13C values of methane and CO2 in the headspace as well as of DIC in the slurries were determined using a Thermo Finnigan Trace GC connected to a DELTA Plus XP IRMS (Thermo Scientific, Bremen, Germany) as described previously [35] ...
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bioRxiv - Evolutionary Biology 2021Quote: ... stable isotope ratios were measured in a single run on a Delta V Advantage isotope ratio mass spectrometer coupled to a Flash 1112 Elemental Analyser via a Conflow III interface (Thermo Scientific Milan, Italy). Results were expressed in δ notation 66 and reported in permille units ...
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bioRxiv - Cancer Biology 2020Quote: ... The specimen itself was floated on the water surface inside a standard multi well plate (Thermo Scientific Nunclon Delta Surface 96 well plates). Those are needed to be tape sealed in order to prevent water creep under ultrasound irradiation ...
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bioRxiv - Plant Biology 2020Quote: ... of the ground material was weighed in tin capsules and converted to CO2 and N2 in an elemental analyzer Euro EA (Hekatech GmbH, Wegberg, Germany) connected to an Isotope Ratio Mass Spectrometer (IRMS Delta V Advantage, Thermo Scientific, Bremen, Germany) to determine C and N contents and the isotopic compositions ...
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bioRxiv - Microbiology 2021Quote: ... connected on-line via a GC-Isolink combustion interface to a ConFlo IV and Delta V Advantage isotope ratio mass spectrometer (GC-IRMS, Thermo Scientific, Bremen, Germany). Both concentrations and δ 13C values of lipids were corrected for the introduction of carbon atoms during derivatization.
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bioRxiv - Microbiology 2022Quote: ... and the negative control PCγC-terminal were prepared in 0.05×PDB in a 96-well microtiter plate (Nunclon Delta, Thermo Fisher Scientific, Waltham, MA, USA) at a volume of 100 μL per well ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... 50 µl of cell supernatant from each well was transferred into a 96-microwell white plate (polystyrene, Nunclon Delta, Nunc, Thermo Fisher Scientific) and served as a measure of basal luminescence levels in each well ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... The nLuc-hPAR1/2/4-eYFP-CHO reporter cells were plated either in a 24-well or 96-well cell culture plates (polystyrene, flat-clear bottom, Nunclon Delta, Nunc, Thermo Fisher Scientific) at a cell density of 5 × 104 cells per well in complete F-12 medium and cultured for 48 h ...
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bioRxiv - Cell Biology 2020Quote: The δ13C value and total carbon mass of each sample was determined in triplicate using a Delta V Advantage Isotope Ratio Mass Spectrometer (Thermo Fisher, Bremen, Germany) configured to a Thermo Finnigan 1112 Series Flash Elemental Analyzer at the University of Oslo ...
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Natural abundance isotope ratios to differentiate sources of carbon used during tumor growth in vivobioRxiv - Cancer Biology 2020Quote: The carbon stable isotope value of each sample was determined using a Delta V Advantage Isotope Ratio Mass Spectrometer (Thermo Fisher, Bremen, Germany) configured with a Thermo Fisher EA Isolink Elemental Analyzer at the University of Oslo ...
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bioRxiv - Biochemistry 2022Quote: Reactivity of recombinant S proteins from Wuhan and VOC Omicron as well as of recombinant RBD (Wuhan, Delta and Omicron) to large set (>9000) of human proteins was assessed by using protein microarrays (ProtoArray®, Invitrogen, Thermo Fischer). First microarray chips were blocked by incubation for 1 hour at 4 °C with buffer containing ...
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bioRxiv - Biophysics 2023Quote: ... They are embedded in basement membrane extract hydrogels (Cultrex RGF, Biotechne, Minneapolis) and placed in a pre-warmed 48-well plate (Nunc delta surface, Thermo Fisher Scientific, Waltham). The organoids are cultured in Advanced DMEM/F12 medium (Gibco ...
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bioRxiv - Microbiology 2023Quote: The isotopic fractions of 15N-labelled nitrogen-oxyanions (NO3- +NO2-) were analysed using a Thermo Delta V isotope ratio mass spectrometer (IRMS, Thermo Fisher Scientific, USA) following conversion to N2O via the denitrifier protocol37 ...
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bioRxiv - Neuroscience 2023Quote: ... Day 25 sensory neurons (SNs) were differentiated in the presence of DMSO or genipin in Nunclon Delta surface dishes (ThermoFisher, cat# 150460 vendor). Cells were washed with PBS and fixed with 4% paraformaldehyde for 20 min at room temperature ...
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bioRxiv - Cell Biology 2022Quote: ... for 3 days at a density of 5 × 104 cells per well in a 96-well tissue culture plate (Thermo Fisher, Nunclon delta). The media was then replaced with fresh PMA-free media and the cells were left to rest for 24 h before the infection protocol ...