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Citations for Thermo Fisher :
251 - 300 of 10000+ citations for Borrelia burgdorferi C t Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: HeLa T-REx cells (Invitrogen) were cultured in DMEM supplemented with 10% FBS (Gibco ...
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bioRxiv - Biophysics 2022Quote: ... T-REx-CHO (Thermo Fisher) and HEK293S GnTI-were used for stable expression ...
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bioRxiv - Systems Biology 2019Quote: The T-Rex system (Invitrogen) was used for tetracycline-dependent expression of human Apcdd1 ...
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bioRxiv - Cell Biology 2021Quote: ... TMRM (ThermoFisher Scientific, T-668) was dissolved in DMSO to make a 10 mM stock ...
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bioRxiv - Molecular Biology 2022Quote: T-Rex-293 cells (Invitrogen) were cultured and maintained as previously described63 in DMEM (Gibco 11-965-092 ...
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bioRxiv - Immunology 2023Quote: ... in T Buffer (Life Technologies). Settings were the following ...
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bioRxiv - Bioengineering 2023Quote: ... T-75 flasks (Fisher Scientific) coated with 0.1 % gelatin (PCS-999-027 ...
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Downregulation of Let-7 miRNA promotes Tc17 differentiation and emphysema via de-repression of RORγtbioRxiv - Immunology 2023Quote: ... ROR gamma T PE (Invitrogen), TCF1 AF647 (Cell Signaling Technologies) ...
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bioRxiv - Physiology 2024Quote: ... in PBS-T (ThermoFisher, 28352), and blotted using antibodies against Cx43 (Sigma ...
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bioRxiv - Microbiology 2022Quote: ... supernatants were stored at - 80°C and protein concentrations were further determined using the Pierce™ BCA Protein Assay Kit (23225, ThermoFisher Scientific).
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bioRxiv - Microbiology 2019Quote: ... Samples were centrifuged at 10,000 x g for 30 min at 4 °C before supernatants were collected and protein content was determined using the BCA Protein Assay Kit (Pierce, Thermo Fisher Scientific) following the manufacturer’s instructions.
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bioRxiv - Physiology 2020Quote: ... and MOTS-c in each group was normalized to the total tissue protein concentration as determined by the Bradford protein assay (Thermo Fisher Scientific).
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bioRxiv - Cell Biology 2019Quote: ... The lysate was centrifuged at (21000 g) for 15 min at 4°C and protein concentration determined (Pierce™ 660nm Protein Assay Reagent, Thermo Scientific). 8.5 μg lysate was kept for western blot analysis and 1.6-1.8 mg total protein was added to 250 μl washed high capacity neutravidin beads (Life Technologies ...
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bioRxiv - Cell Biology 2021Quote: ... Lysates were centrifuged at 14000 rpm for 15 minutes at 4°C and protein concentration determined with a Pierce™ BCA Protein Assay Kit (Life Technologies). High capacity neutravidin beads (Life Technologies ...
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bioRxiv - Systems Biology 2020Quote: ... Lysates were clarified by centrifugation at 10,000 rpm for 2 minutes at 4°C and snap frozen in liquid nitrogen with protein concentrations measured using the BCA protein assay (Pierce/Thermo Fisher Scientific). For RAS activation assays ...
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bioRxiv - Neuroscience 2022Quote: ... Proteins were denatured by boiling at 95°C for 10 mins and subjected to a PierceTM BCA protein assay (Life Technologies, 23227) for determination of the protein concentration ...
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bioRxiv - Microbiology 2020Quote: ... Pre-cleared lysates were further incubated at 4°C overnight with the indicated antibodies (1 to 3 μl) and protein A agarose or protein G Dynabeads (Thermo Fisher Scientific). Immunoprecipitates were washed three times with RIPA buffer (LSB ...
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bioRxiv - Microbiology 2020Quote: ... Lysates were clarified by centrifugation (12,000 g for 30 min at 4 °C) and protein concentrations were determined using a BCA protein assay kit (Thermo Fisher Scientific). SOD activity in whole cell extracts was measured using the EnzyChrome superoxide dismutase assay kit (BioAssay Systems ...
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bioRxiv - Cell Biology 2020Quote: ... Cell debris were removed by centrifugation at 1000 × g for 5 min at 4°C and the protein concentration of the resulting supernatant was estimated using the BCA protein assay kit (Thermo Fisher Scientific) with bovine serum albumin as the standard ...
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bioRxiv - Genomics 2022Quote: ... 10 min at 4°C and supernatants quantified for protein content using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific, 23227) according to the manufacturer’s instructions.
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bioRxiv - Neuroscience 2022Quote: ... Samples were cleared by centrifugation at 4°C and 23,000×g for 30 min and protein concentration determined using Pierce(tm) BCA Protein Assay (Thermo Fisher Scientific, USA). 0.2 μg/μl α-casein were added to a protein amount of 1.2 mg ...
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bioRxiv - Developmental Biology 2023Quote: ... Whole cell lysates were centrifuged for 15 min at maximum speed at 4°C and protein concentrations measured from the supernatant with a Pierce BCA Protein Assay Kit (Thermo Scientific, 23227). 15 µg protein per lane were resolved on 8% SDS-PAGE ...
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bioRxiv - Neuroscience 2023Quote: Samples (input (eluted in 2× SDS buffer and thawed 15 minutes at 75°C) and IP proteins) were run in NuPAGE 4%–12% Bis-Tris Mini Protein Gel (10 wells) (ThermoFisher Scientific, NP0321BOX) at the voltage of 100V for 2.5 hours in a NuPAGE MOPS SDS Running buffer (ThermoFisher ...
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bioRxiv - Cell Biology 2023Quote: ... Lysates were cleared by centrifuging at 12.000 rpm for 5 min at 4°C and protein concentration determined using the Pierce™ BCA Protein Assay (Thermo Scientific 23225). Samples were prepared for SDS-Page in 1× NuPAGE™ LDS Sample Buffer containing 50mM DTT ...
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bioRxiv - Biochemistry 2023Quote: ... The lysates were then centrifuged for 15 min at 16,000 × g at 4°C and their protein concentrations were determined using a BCA Protein Assay Kit (#23227, Thermo Fisher Scientific, USA), according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... Lysates were then centrifuged at 10,000 rpm for 10’ at 4°C and the supernatant was collected to determine protein concentration using Pierce BCA Protein Assay Kit (#23225, Thermo Fisher Scientific). Before loading ...
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bioRxiv - Cell Biology 2023Quote: Cells were lysed in 1x RIPA Buffer pre-warmed to 95°C and the total protein concentration was normalised using the Pierce BCA Protein Assay (Cat. # 23227; Thermo Fisher Scientific). Proteins (20 µg ...
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bioRxiv - Plant Biology 2023Quote: ... The lysate was cleared by centrifugation (18,000 x g for 10 min at 25°C) and the protein content of the supernatant was determined using the Pierce BCA protein assay kit (Thermo Fisher Scientific). Reduction ...
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bioRxiv - Cancer Biology 2022Quote: ... T cells were either cultured alone or with T cell activation beads (Dynabeads™ Mouse T-Activator CD3/CD28 for T-Cell Expansion and Activation, Thermo Fisher Scientific, 11456D). After 72 hrs of culture ...
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bioRxiv - Cancer Biology 2022Quote: ... T cells were either cultured alone or with T cell activation beads (Dynabeads™ Mouse T-Activator CD3/CD28 for T-Cell Expansion and Activation, Thermo Fisher Scientific, 11456D). After 72 hrs of culture ...
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bioRxiv - Genomics 2021Quote: ... FACS sorted BST2+ T cells and BST2− T cells were stimulated with Dynabeads™ Human T-Activator CD3/CD28 for T Cell Expansion and Activation (ThermoFisher, cat#11161D, USA). Briefly ...
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bioRxiv - Immunology 2022Quote: MACS enriched CD4+ T cells and CD3+ T cells were stimulated with Dynabeads™ Human T-Activator CD3/CD28 for T Cell Expansion and Activation (ThermoFisher, Cat# 11131D, USA) following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... CD8+ T-cells were then activated with Dynabeads™ Human T-Activator CD3/CD28 for T Cell Expansion and Activation (Thermo Scientific, Cat. #11161D) at a concentration of 25 μL/1 x 106 cells ...
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bioRxiv - Cell Biology 2019Quote: Reducing SDS-PAGE: Nematodes were synchronized in young adult stage and homogenized in a T-PER Tissue Protein Extraction Reagent (Thermo Scientific) with Halt Protease and Phosphatase Inhibitor Cocktail (Thermo Scientific) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... 250 µL of pre-chilled complete protein extraction buffer (pre-chilled T-PER tissue extraction reagent [ThermoFisher Scientific] with 1×Halt protease inhibitor [ThermoFisher Scientific]) was added per sample tube ...
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bioRxiv - Neuroscience 2021Quote: ... hippocampal tissue was homogenized in a lysis buffer T-PER® Tissue Protein Extraction Reagent (Thermo Fisher Scientific, Waltham, MA, USA) containing a protease inhibitor cocktail (Nacalai Tesque ...
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bioRxiv - Molecular Biology 2019Quote: ... membranes were washed three times with TBS-T before detection of proteins of interest with Pierce-ECL western blot substrate (Thermo Scientific) or Luminata Crescendo Western HRP substrate (EMD-Millipore ...
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bioRxiv - Immunology 2019Quote: Intestinal homogenates were prepared as described30 with minor modifications: small intestinal samples were homogenized with 20 mL/g of buffer (T-Per Tissue Protein Extraction Reagent, Thermo Scientific) containing protease inhibitors (Sigma) ...
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bioRxiv - Molecular Biology 2019Quote: ... Protein A magnetic beads were pre-blocked for 1 hour at RT with 70 μg Yeast t-RNA (Thermo Fisher Scientific). Sequencing libraries were prepared using the NEBNext DNA ultra kit (E7370 ...
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bioRxiv - Immunology 2019Quote: ... 30-40 mg of tissue were transferred to a clean tube and added 400 µL T-PER tissue protein extraction reagent (Thermo Scientific) buffer including proteinase inhibitors (Sigma-Aldrich) ...
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bioRxiv - Neuroscience 2021Quote: ... 500 µg of total proteins of mouse brain homogenates and primary cultures and 10 µg of total proteins of activated T lymphocytes were incubated overnight with 50 µL of Dynabeads™ (ThermoFisher) previously conjugated with 4-10-C9 antibody ...
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bioRxiv - Molecular Biology 2021Quote: ... we extracted proteins from the outer cortex of kidney (did not include medulla) using a T-per buffer (ThermoFisher Scientific 78510) including with a protease inhibitor cocktail (Cell Signaling Technology 5871S ...
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bioRxiv - Physiology 2022Quote: ... To isolate proteins, tissues were homogenized using a handheld homogenizer (Omni International, Kennesaw, GA, USA) in T-PER tissue extraction reagent (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2022Quote: Vero cells were infected with each of the indicated viruses at an MOI of 5 for 24 h and lysed with T-PER Tissue Protein Extraction Reagent (Thermo Scientific). The lysates were sonicated and denatured with Glycoprotein Denaturing Buffer (NEB ...
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bioRxiv - Neuroscience 2023Quote: Luciferase assays: Protein was extracted from tissue samples using the lysis buffer T-Per with 1X Halt protease inhibitor (Invitrogen, 78430) while kept ice cold at all times ...
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bioRxiv - Bioengineering 2024Quote: ... Organs were homogenized using a FastPrep tissue homogenizer (MP Bio) in Lysing Matrix D tubes (MP Bio) containing Tissue Protein Extraction buffer (T-PER, Thermo Scientific) supplemented with protease inhibitor tablets (Roche) ...
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bioRxiv - Cell Biology 2023Quote: ... Brain slices were then incubated either 4 hours at room temperature or overnight at 4°C in 1.5% Normal Donkey Serum in PBS-T with donkey anti-rat DyLight 550 (ThermoFisher, SA5-10029, 1:1000), donkey anti-goat AlexaFluor 488 (Jackson ImmunoResearch ...
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bioRxiv - Cancer Biology 2023Quote: ... Equal amounts of RNA were reverse transcribed and amplified using TaqMan™ RNA-to-C T ™ 1-Step Kit kit on the ViiA 7 Real-Time PCR System (Applied Biosystems). TaqMan gene expression assay probes (Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2021Quote: ... We then stimulated T cells with Dynabeads™ Human T-Activator CD3/CD28 from ThermoFisher overnight with a ratio of beads to cells as 1:3 ...
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bioRxiv - Cancer Biology 2022Quote: ... T cells were activated and expanded with Dynabeads Mouse T-Activator CD3/CD28 (Thermo Fisher) per manufacturer’s protocol ...