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Citations for Thermo Fisher :
151 - 200 of 10000+ citations for Borrelia burgdorferi C t Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... Cell debris were pelleted by centrifugation (14,000g, 10min, 4°C) and whole cell protein extract concentration was measured using Qubit Protein Assay Kit (Thermo Fisher). Protein extracts were immediately used for EnzyChrom Catalase Assay Kit and EnzyChrom Glutathione Reductase Assay Kit (BioAssay Systems) ...
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bioRxiv - Neuroscience 2020Quote: ... Protein samples were heated to 70°C and loaded on 3-8% Tris-Acetate Protein Gels (ThermoFisher Scientific, Waltham, MA) or NuPAGE 4-12% Bis-Tris Protein Gels (ThermoFisher Scientific ...
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bioRxiv - Cancer Biology 2021Quote: ... Protein-containing supernatants were pre-cleared for 1 h at 4°C with Dynabead® protein G (Thermo Fisher Scientific). Pre-cleared supernatants were incubated overnight at 4°C with 1:1250 dilution of Rabbit (DAIE ...
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bioRxiv - Molecular Biology 2019Quote: ... Antibody-bound chromatin was captured with magnetic beads for 1 h at 4°C (50 μl 1:1 mixture of Dynabeads Protein A and Dynabeads Protein G, Invitrogen). DNA from both input and ChIP samples was then extracted using phenol/chloroform ...
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bioRxiv - Molecular Biology 2019Quote: ... Immuno-precipitation was done overnight at 4°C using Protein A-coupled or Protein G-coupled Dynabeads previously incubated with anti-GFP A11122 antibody (Invitrogen), anti-Flag antibody (M2 Sigma ...
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bioRxiv - Cancer Biology 2023Quote: ... One mg of total protein/sample were incubated for 6 h at 4°C with protein A/G-magnetic beads (Dynabeads, Invitrogen) prebound with 5 μg of anti-mCherry antibody ...
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bioRxiv - Cancer Biology 2023Quote: ... One mg of total protein/sample were incubated for 6 h at 4°C with protein A/G-magnetic beads (Dynabeads, Invitrogen) prebound with 5 μg of the corresponding antibody ...
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bioRxiv - Cancer Biology 2024Quote: ... The insoluble fraction was removed via centrifugation (13,000xg, 10 min, 4 °C) and protein abundance was quantified by BCA Protein Assay (Thermo Scientific). 50 μg of protein was utilized per well in a total volume of 60 μL ...
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bioRxiv - Cell Biology 2023Quote: ... Antibody–protein complexes were recovered during 2 h incubation at 4°C with protein A/G magnetic beads (Thermo Scientific), followed by duplicate washes of 15 min with low-salt wash buffer (0.1% SDS ...
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bioRxiv - Immunology 2022Quote: Cells and mouse tissues were lysed in M-PER or T-PER Tissue Protein Extraction Reagent (Thermo Fisher Scientific) containing additional 1X PhosStop (Sigma ...
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bioRxiv - Cell Biology 2024Quote: ... Western blotting was performed with Tris-buffered saline and 0.2% Tween-20 (TBS-T) using PageRuler Prestained protein ladder (10-180 kDa formulation; ThermoFisher) and the following primary and secondary antibodies ...
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bioRxiv - Immunology 2019Quote: ... Human T-cells isolated were also used to generate T-cells blasts using the Dynabeads Human T-Activator CD3/CD28 for T-cell expansion and Activation kit (Life Technologies) as per company’s protocol ...
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bioRxiv - Neuroscience 2021Quote: ... Suspension HEK293S GnTI− cells expressing dolphin Prestin were grown at 37 °C and 7.8% CO2 in t FreeStyleTM 293 expression medium (Gibco Fisher Scientific) supplemented with 2% heat-inactivated fetal bovine serum (FBS ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were blocked in PBS-T containing 5% NDS and then incubated overnight at 4°C with rabbit anti-PSD95 (1:1000, Invitrogen/ThermoFisher) and guinea pig anti-vGlut2 (1:10,000 ...
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bioRxiv - Microbiology 2021Quote: ... and incubated with primary antibodies (1% BSA/PBS-T, overnight at 4°C) at the following dilutions: mouse anti-SAG1 (1:1000, Thermo Scientific) and rat anti-HA (1:1000 ...
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bioRxiv - Cancer Biology 2021Quote: ... slides were incubated overnight in a humidified chamber at 4 °C in TBS-T with 1% Fatty acid free-BSA (Fisher Scientific) with anti-mouse/human antibodies ...
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bioRxiv - Immunology 2021Quote: ... T cells were isolated from spleens of Balb/c mice as described above and subsequently labeled with CFSE (ThermoFisher, cat. C34554) per the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: HEK293T and Flp-In T-REx 293 cells were maintained in 37°C and 5% CO2 in Dulbecco’s Modified Eagle Medium (Thermo Fisher, 41966029) supplemented with 10% Fetal Bovine Serum (Thermo Fisher ...
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bioRxiv - Genetics 2019Quote: ... 15 minutes each at 4°C) in PBS-T 0.05% and incubated with either an Alexa Fluor 488 goat anti-rat IgG (Thermo Fisher Scientific) or an Alexa Fluor 488 goat anti-rabbit IgG (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2022Quote: ... Brains were extracted from the skull and post-fixed in 4% PFA for 12-36 hours and then they were cryoprotected in 30% sucrose for >24 hours and embedded in optimal cutting temperature mounting medium (Fisher Healthcare Tissue-Plus O. C. T. Compound, Fisher Scientific) for freezing over dry ice ...
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Mitochondrial Apolipoprotein MIC26 is a metabolic rheostat regulating central cellular fuel pathwaysbioRxiv - Cell Biology 2023Quote: ... washed three times with TBS-T and probed at 4°C overnight with the following primary antibodies: MIC26 (Invitrogen, 1:1000), MIC27 (Sigma-Aldrich ...
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bioRxiv - Immunology 2024Quote: ... Efferocytosis was quantified by incubating BMDMs with Jurkat T cells (MOI 2, 45 min) which had been labelled with calcein-acetoxymethyl (2 h, 37 °C, ThermoFisher Scientific) and exposed to UV light (305 nm ...
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bioRxiv - Immunology 2021Quote: ... T cells were then activated by CD3/CD28 T-activator Dynabeads (11131D Gibco) and co-cultured with eosinophils at a 1:1 ratio (2×105 total ...
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bioRxiv - Molecular Biology 2019Quote: ... T Cells were stimulated with Dynabeads™ Human T-Expander CD3/CD28 (Gibco). The cells were cultured in GRex (Wilson Wolfe ...
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bioRxiv - Immunology 2020Quote: ... T cells were stimulated with Dynabeads® Human T expander CD3/CD28 (Invitrogen) at a 1:3 ratio (T cell:bead) ...
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bioRxiv - Biochemistry 2020Quote: Fine chemicals were procured as follows: Thioflavin T (Th T) from Acros Organics, Belgium ...
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Mapping of the autophagosomal degradome identifies IL-7Rα as key cargo in proliferating CD4+ T-cellsbioRxiv - Immunology 2021Quote: ... CD4+ T cells were stimulated with Dynabeads Mouse T-Activator CD3/CD28 (Gibco) and recombinant murine IL-2 (20 ng/ml ...
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bioRxiv - Immunology 2021Quote: ... T-cells were stimulated with Dynabeads Human T-Activator CD3/CD28 (11131D; Gibco) and T-cell proliferation was assessed 4 days later by CellTrace™ Violet dilution by flow cytometry.
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bioRxiv - Microbiology 2022Quote: ... CD4+ T cells were activated using Human T-Activator CD3/CD28 Dynabeads (Invitrogen) according to the manufacturer’s instructions and maintained in RPMI GlutaMax supplemented with 10% FCS and 30 U/ml recombinant IL-2 (Roche).
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bioRxiv - Immunology 2023Quote: ... T cell activation was performed using Human T-Expander CD3/CD28 Dynabeads (Gibco) at a 1.5:1 bead:cell ratio in complete medium for 3 days ...
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bioRxiv - Synthetic Biology 2024Quote: ... T cells were activated using Human T-Activator CD3/CD28 Dynabeads (Thermo Fisher) at a 1:1 cell:bead ratio in T cell growth medium ...
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bioRxiv - Immunology 2023Quote: ... T cells were activated with Human T-Activator CD3/CD28 Dynabeads (Thermo Fisher) at a 1:1 bead:cell ratio in RPMI (Gibco ...
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bioRxiv - Immunology 2023Quote: Purified T cells were activated with Dynabeads Human T-Activator CD3/CD28 (ThermoFisher) at a 1:1 bead:cell ratio in complete RPMI supplemented with 5 ng/mL IL-7 and IL-15 (both PeproTech ...
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bioRxiv - Synthetic Biology 2023Quote: Mixed CD4+ and CD8+ primary T cells were isolated with the Dynabeads™ Human T-Activator CD3/CD28 for T Cell Expansion and Activation (Thermo Fisher) from healthy donor whole blood obtained from the Stanford Blood Center (Stanford ...
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bioRxiv - Molecular Biology 2021Quote: ... antibodies overnight at 4°C and then incubated for an additional 3 hours at 4°C with 50 μL DynabeadsTM protein G (Life Technologies, 10004D). Following three washes with 1% Triton-TBS lysis buffer ...
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bioRxiv - Biochemistry 2021Quote: ... and protein was quantified in the supernatant for temperatures 37°C and 41°C by micro BCA assay (Thermo Fisher Scientific - Pierce). Following quantification ...
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bioRxiv - Cancer Biology 2021Quote: ... and protein was quantified in the supernatant for temperatures 37°C and 41°C by micro-BCA assay (Thermo Fisher Scientific - Pierce). Following quantification ...
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bioRxiv - Cancer Biology 2020Quote: ... The supernatant was stored at −20°C for protein quantification performed using Pierce ™ BCA Protein Assay Kit (Thermo Fisher, 23227).
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bioRxiv - Genomics 2020Quote: ... diluted chromatin was incubated overnight at 4°C with Protein A and Protein G Dynabeads (ThermoFisher Scientific 10002D, 10004D; 25µL each) and indicated antibodies (Key Resources Table) ...
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bioRxiv - Systems Biology 2021Quote: ... Lysates were cleared by centrifugation at 16,000 x g for 10 minutes at 4°C and protein concentration was determined using the BCA protein assay kit (Thermo Scientific). Fifty micrograms of protein was reduced with 5 mM Tris 2-carboxyethylphosphine (TCEP ...
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bioRxiv - Cell Biology 2021Quote: ... Lysates were clarified by centrifugation (20000 g, 10 minutes, 4 °C) and protein concentrations were determined using the BCA protein assay kit (ThermoFisher Scientific). The same amount of protein (1.2 μg ...
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bioRxiv - Cell Biology 2021Quote: ... Lysates were pelleted for 15 min at 13 000 xg at 4 °C and supernatants kept for protein quantification (BCA Protein Assay Kit, Thermo Scientific). Equal amounts of cellular proteins were resolved on 10% or 12% sodium dodecyl sulfate – polyacrylamide gels (SDS-PAGE ...
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bioRxiv - Cancer Biology 2022Quote: ... Cellular debris was pelleted for 15 minutes at 4 °C and protein concentration was quantitated with the Coomassie (Bradford) Protein Assay (ThermoFisher Scientific). A total of 15μg (organoids ...
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bioRxiv - Immunology 2022Quote: Expression plasmids encoding His-tagged SARS-CoV-2 RBD (residues 319 to 541 of SARS-CoV-2 S protein) or S protein (with a C-terminal trimerization domain) were cloned into pCEP4 vector and transfected into Expi293F (ThermoFisher Scientific) and the proteins expressed for 7 days at 37°C (Rouet et al. ...
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bioRxiv - Neuroscience 2022Quote: Cell culture supernatants from cerebral cortex neurons were centrifuged at 10,000 g and 4°C and subjected either to protein precipitation or to immunoprecipitation using Reelin antibody G10 and Dynabeads Protein G (ThermoFisher Scientific).
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bioRxiv - Molecular Biology 2022Quote: ... Samples were heated at 60°C for 15 min and protein concentration was determined by BCA assay (Pierce BCA Protein Assay Kit, Thermo Scientific). The protein mixture (30∼50 µg ...
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bioRxiv - Molecular Biology 2022Quote: ... Cell lysates were cleared by centrifugation at 16,000 × g for 5 min at 4°C and protein concentration was determined using the Pierce BCA protein assay kit (Thermo Scientific). Cell lysates were mixed with Laemmli sample buffer (Alfa Aesar ...
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bioRxiv - Cell Biology 2019Quote: Cells were lysed in 1x Laemmli Buffer pre-warmed to 95°C and the total protein concentration was normalised using the Pierce BCA Protein Assay (Cat. # 23250; ThermoFisher Scientific). Proteins (10µg/sample ...
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bioRxiv - Genetics 2021Quote: ... The lysate was incubated for 2 hours in a rotating wheel at 4°C with Dynabeads Protein A (#10002D Dynabeads Protein A, ThermoFisher Scientific) as a preclearing step ...
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bioRxiv - Immunology 2020Quote: ... Lysates were cleared by centrifugation at 20,000 g for 15 min at 4°C then protein concentrations determined using Coomassie Protein Assay Kit (Thermo Scientific, UK). For each sample ...