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Citations for Thermo Fisher :
101 - 150 of 10000+ citations for Borrelia burgdorferi C t Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Targeted degradation of PCNA outperforms stoichiometric inhibition to result in programed cell deathbioRxiv - Cell Biology 2022Quote: Human embryonic kidney T-REx 293 cells which express the tetracycline repressor protein were purchased from Thermo Fisher Scientific and cultured in Eagle’s Minimum Essential Medium (MEM ...
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bioRxiv - Neuroscience 2020Quote: Frozen brain samples and peripheral organs were homogenized in T-PER™ Tissue Protein Extraction Reagent (Thermo Fisher), N13 cells were lysed in RIPA buffer (Thermo Scientific) ...
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bioRxiv - Physiology 2022Quote: ... ground using mortar and pestle and lysed in T-PER Tissue Protein Extraction Reagent (Thermo Fisher Scientific, #78510) with Halt Protease and Phosphatase Inhibitor Cocktail (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2022Quote: PVDF membranes containing transferred proteins were incubated in blocking buffer (1 x TBS-T (Thermo Fisher Scientific, 28360), 5% Blotting-Grade Blocker (BioRad ...
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A nepenthesin insert allosterically controls catalysis in the malaria parasite protease plasmepsin VbioRxiv - Microbiology 2022Quote: ... Lysates were then incubated for one hour at 4°C with Dynabeads-Protein A or Dynabeads-Protein G (ThermoFisher) that had been bound to anti-GFP (clone 3E6 ...
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bioRxiv - Neuroscience 2019Quote: ... Myc-tagged proteins were immunoprecipitated using anti-c-Myc monoclonal antibody supernatant (9E10, DSHB) and protein A-agarose (Invitrogen). Flag-tagged proteins were immunoprecipitated using anti-Flag-M2 affinity gel (Sigma) ...
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bioRxiv - Developmental Biology 2020Quote: ... Samples were denatured at 95°C for 10min and protein quantity was measured using Pierce 660nm Protein Assay (ThermoFisher). Protein extracts (10µg ...
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bioRxiv - Cancer Biology 2022Quote: ... Lysates were cleared of insoluble material by centrifuging at 20,000 g for 10 min at 4°C and protein concentration was determined with the BCA Protein Assay (ThermoFisher). For immunoblotting 20-40 μg of protein in equal volumes were heated in LDS-sample buffer (Invitrogen ...
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bioRxiv - Cancer Biology 2023Quote: ... Lysates were clarified by centrifugation at 4°C and the protein concentration was determined by BCA protein assay (Thermofisher). Samples were resolved on a precast 4%–12% gradient gel (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... 6000 rpm 4°C and protein concentration was estimated by BCA protein assay kits (Thermo-Fisher Scientific, Cat #23225).
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bioRxiv - Genetics 2021Quote: ... DSF was performed by heating samples containing 10X SYPRO Orange in 1°C increments from 25°C to 100°C using the StepOne real-time PCR machine and using the Protein Thermal Shift software (ThermoFisher) to fit the data.
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bioRxiv - Cell Biology 2021Quote: ... 100 μL reactions containing 10 μL FLAG-xCENP-C mutants in interphase CENP-C-depleted extract were added to 10 μL Protein A Dynabeads (Invitrogen) coupled to 2.5 μg mouse anti-FLAG antibody ...
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bioRxiv - Cell Biology 2021Quote: ... 100 μL reactions containing 10 μL Myc-xCENP-C truncations in interphase CENP-C-depleted extract were added to 10 μL Protein A Dynabeads (Invitrogen) coupled to 2.5 μg mouse anti-Myc antibody ...
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bioRxiv - Neuroscience 2021Quote: ... Suspension HEK293S GnTI− cells expressing dolphin Prestin were grown at 37 °C and 7.8% CO2 in t FreeStyleTM 293 expression medium (Gibco Fisher Scientific) supplemented with 2% heat-inactivated fetal bovine serum (FBS ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were blocked in PBS-T containing 5% NDS and then incubated overnight at 4°C with rabbit anti-PSD95 (1:1000, Invitrogen/ThermoFisher) and guinea pig anti-vGlut2 (1:10,000 ...
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bioRxiv - Cell Biology 2022Quote: ... HeLa cells (CCL-2, ATCC) were maintained in T-75 flasks (Falcon) at 37 °C and 5% CO2 in DMEM (Gibco) supplemented with 4.5 g/L glucose ...
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bioRxiv - Neuroscience 2019Quote: ... Sections were transferred to well plates containing primary antibodies made in PBS-T (1:1000 rabbit anti-c-Fos [SySy]; 1:5000 chicken anti-GFP [Invitrogen]) and incubated on a shaker at 4°C for 48 hrs ...
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bioRxiv - Immunology 2021Quote: Thymocytes and LN T cells were cultured at 37°C with 5% CO2 in RPMI-1640 (Gibco, Invitrogen Corporation, CA) supplemented with 10% (v/v ...
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bioRxiv - Immunology 2021Quote: Thymocytes and LN T cells were cultured at 37°C with 5% CO2 in RPMI-1640 (Gibco, Invitrogen Corporation, CA) supplemented with 10% (v/v ...
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bioRxiv - Neuroscience 2021Quote: ... and p.Arg288Ter iPSCs (from ST3GAL5 c.862C>T (p.Arg288Ter) patient fibroblasts) was performed using the CytoTune-iPS Sendai Reprogramming Kit (Invitrogen, # A13780) and the CytoTune-iPS 2.0 Sendai Reprogramming Kit (ThermoFisher ...
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bioRxiv - Immunology 2022Quote: ... cells were cultured at 37°C in a temperature- and CO2-controlled incubation chamber in complete T cell medium without phenol red (RPMI-1640 (Gibco), 2 mM L-Glutamine (Bioconcept) ...
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bioRxiv - Microbiology 2023Quote: ... The membrane was then incubated in primary antibody in 5% milk and TBS-T overnight at 4°C at the following dilutions: 1:1000 rabbit αFLAG polyclonal (Invitrogen) and 1:1000 rabbit αHsp70 polyclonal (Abcam) ...
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bioRxiv - Biochemistry 2022Quote: ... diluted in PBS-T overnight at 4°C and detected with 1:2000 HRP-conjugated goat anti-chicken secondary antibody (#A16054, Invitrogen) diluted in PBS-T during 1 hour at room temperature ...
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bioRxiv - Immunology 2023Quote: ... were either cocultured with irradiated (20Gy) BALB/c mice BMDCs (5 × 104) or activated using mouse T-activator CD3/CD28 Dynabeads (Gibco) at a 1-to-1 bead-to-cell ratio ...
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bioRxiv - Biochemistry 2023Quote: U2 OS and HEK293-T cells were grown at 37 °C in 5 % CO2 in DMEM low glucose (Gibco 11885) supplemented with 10 % heat inactivated FBS (F4135 ...
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bioRxiv - Immunology 2023Quote: Thymocytes and LN T cells were cultured at 37°C with 5% CO2 in RPMI-1640 (Gibco, Invitrogen Corporation, CA) supplemented with 10% (v/v ...
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bioRxiv - Immunology 2023Quote: Thymocytes and LN T cells were cultured at 37°C with 5% CO2 in RPMI-1640 (Gibco, Invitrogen Corporation, CA) supplemented with 10% (v/v ...
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bioRxiv - Immunology 2023Quote: ... Cells were grown at 37°C with 5% CO2 in T-175 flasks (CELLSTAR Flasks, USA Scientific, Ocala, FL) in minimal essential medium (MEM; Gibco catalog number 11090073 ...
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bioRxiv - Genetics 2024Quote: ... MDCK cells were transfected with overexpression vectors containing either wild-type or mutant (c.1043C > T) ACTB in Opti-MEM (Gibco) using a NEPA21 electroporator (Nepagene) ...
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bioRxiv - Cancer Biology 2022Quote: ... CD8+ T cells were activated with Dynabeads Mouse T-Activator CD3/CD28 for T-Cell Expansion and Activation (Gibco, 11456D) without exogenous recombinant IL2 supplementation ...
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bioRxiv - Immunology 2022Quote: ... T cells were mixed with Dynabeads Human T-Activator CD3/CD28 (Gibco) at a 2:1 ratio in a 24 well plate in RPMI 1640 medium (Gibco ...
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bioRxiv - Bioengineering 2023Quote: ... T cells were activated with Dynabeads Human T-Activator CD3/CD28 (ThermoFisher) with a T cell:Beads ratio at 1:1 ...
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bioRxiv - Biochemistry 2024Quote: ... CCR6+ T cells were activated with Dynabeads Human T-activator (Gibco, 11132D) at a density of 1 × 106 cell/ml in growth media (RPMI1640 media with 10% serum ...
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bioRxiv - Biochemistry 2022Quote: ... 4°C and total protein determined by the BCA method (Thermo Fisher Scientific). Proteins were separated on a Bolt 4–12% Bis-Tris Plus Gel (Thermo Fisher Scientific) ...
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bioRxiv - Genomics 2022Quote: ... was carried out overnight at 4°C using Protein A Dynabeads (ThermoFisher Scientific). The mononucleosomal fraction (147 bp ...
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bioRxiv - Immunology 2022Quote: ... or mouse anti-Hepatitis C virus Core protein (clone C7-50, Life Technologies) were visualised using IRDye 800CW Donkey anti-Rabbit IgG or Goat anti-Mouse IgG (LiCor) ...
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bioRxiv - Bioengineering 2023Quote: ... protein expression was induced overnight at 16°C with 0.2mM IPTG (Thermofisher, 15529019). The overnight cultures were lysed and sonicated in non-denaturing conditions ...
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bioRxiv - Cell Biology 2023Quote: ... and protein concentrations were quantified using a CBQCA Quantification kit (C-6667, Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... Proteins were purified via CaptureSelect™ C-tag affinity matrix (Thermo Fisher Scientific). A further SEC polishing step was performed on a HiLoad 16/60 Superdex 200 pg column (GE Healthcare ...
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bioRxiv - Biochemistry 2023Quote: ... Proteins were eluted by incubation at 95°C in NuPAGE Loading Buffer (Invitrogen) for 5 minutes ...
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bioRxiv - Biochemistry 2024Quote: ... The proteins were expressed overnight at 18°C in Terrific Broth (Thermo Scientific) and 0.4% v/v glycerol with 0.5 mM isopropyl ß-D-1-thiogalactopyranoside (IPTG ...
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bioRxiv - Microbiology 2024Quote: ... Immunoprecipitations were performed overnight at 4°C with Dynabeads Protein G beads (Invitrogen) conjugated to mouse anti-FLAG M2 (Sigma-Aldrich) ...
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bioRxiv - Developmental Biology 2022Quote: ... (Life Technologies, #T-13320), diluted 1 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: HEK293F/T (Life Technologies), HEK293G (Promega) ...
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bioRxiv - Cell Biology 2021Quote: ... T-Rex-293 (Invitrogen), IMCD3 Flp-In ...
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bioRxiv - Immunology 2023Quote: ... T-bet (4B10),(Invitrogen) CD8β (H35-17.2) ...
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bioRxiv - Cancer Biology 2021Quote: ... After clearing by centrifugation at 13,300□rpm for 10□min at 4°C the protein concentration was determined using Coomassie Plus Protein Assay (ThermoFisher Scientific). Protein concentrations were adjusted to a final concentration of 2mg/ml followed by denaturation upon addition of 4X sample buffer containing 10% Beta-mercaptoethanol and heating to 95oC for 5 minutes ...
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bioRxiv - Molecular Biology 2020Quote: ... The suspension was incubated at 25°C for an additional 2 hr with Protein G SepharoseTM 4 Fast Flow or DynabeadsTM Protein G (Invitrogen), pre-blocked with 1 mg/ml BSA and 0.25 mg/ml Salmon Sperm DNA ...
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bioRxiv - Cancer Biology 2019Quote: ... Lysates were then clarified by spinning at 14,000 rpm at 4°C and protein concentration quantified by BCA Protein Assay (23227, Thermo Scientific). Forty micrograms of proteins were denatured in boiling SDS sample buffer and resolved on 4%-20% gradient gels (5671093 ...
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bioRxiv - Microbiology 2019Quote: ... The samples were heated at 95°C for 10 minutes and centrifuged at 4°C for 10 minutes to remove potential protein aggregates before proteins were separated in gradient polyacrylamide gels (4-12%) (Invitrogen). Proteins were transferred onto nitrocellulose membranes (Whatman ...