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Citations for Thermo Fisher :
251 - 300 of 317 citations for Borrelia Garinii Antigen since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... The phage library was incubated with biotinylated CD40-Fc fusion protein or Her2 recombinant protein (Acro biosystems) for 2 hours at RT and the phage-antigen complex was captured by Dynabeads M280 (Life technologies). The bound phages were eluted by Glycin-HCl (pH 2.2 ...
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bioRxiv - Bioengineering 2020Quote: ... and subjected to heat-induced antigen retrieval using sodium citrate buffer(10 mM, pH 6, Fisher Scientific International, Inc., Pittsburgh, MA) at >80°C ...
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bioRxiv - Neuroscience 2021Quote: ... Immunoreactivity to the antigen was visualized using Alexa Fluor 488- and Alexa Fluor 594-conjugated secondary antibodies (Molecular Probes, Eugene, OR). Nuclear staining was performed with Hoechst 33258 (Polysciences ...
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bioRxiv - Immunology 2022Quote: ... B/Colorado/06/2017) were chosen as a historical antigen and were biotinylated using an EZ-Link Micro NHS-PEG4 Biotinylation Kit (Thermo Fisher) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... with the following modifications (1) antigen retrieval was performed using low pH IHC Ag Retrieval Solution (Thermo Fisher, 00-4955-58) and subjected to heat using a steamer for 40 minutes ...
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bioRxiv - Immunology 2022Quote: ... the cells were first stained for live/dead discrimination followed by surface antigen staining and subsequently fixed using Foxp3 fixation/permeabilization buffer (Thermo Fisher) for 1 hour at 4°C ...
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bioRxiv - Immunology 2019Quote: ... mice were inoculated s.c with overnight ES antigens emulsified with an equal volume of Alum-an aluminum salt adjuvant (88) obtained from Thermo Scientific to achieve a vaccine dose of 100 μg ES in 100 μl Alum as a positive control ...
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bioRxiv - Microbiology 2020Quote: ... LAM antigen was diluted to the desired concentration in control human serum in high-recovery glass vials (Thermo Scientific, Waltham, MA) C5000-995 and incubated overnight (18−24 hrs ...
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bioRxiv - Immunology 2021Quote: ... Cytokine concentrations in the day 6 supernatants of antigen-stimulated cultures were assessed by Luminex bead-based assay (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... and fusion constructs of these antigens to the full length mouse invariant chain (CD74) (NCBI RefSeq: NC_000084.6) were obtained from GeneArt (Life technologies, Paisley, UK). The fusion Thyβ4-CD74 and Thyβ10-CD74 constructs were designed as an in-frame fusion of N-terminus of the thymosin cDNA sequences to the C-terminus of the full length murine invariant chain ...
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bioRxiv - Microbiology 2020Quote: ... The antigens were then reacted with an Alexa Fluor 488-conjugated goat anti-mouse IgG H+L antibody (Thermo Fisher Scientific). The antibody titer was defined as the reciprocal of the highest dilution level at which a specific fluorescent signal was detected.
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bioRxiv - Immunology 2021Quote: ... B/Colorado/06/2017) were chosen as a historical antigen and were biotinylated using an EZ-Link Micro NHS-PEG4 Biotinylation Kit (Thermo Fisher) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... Antibodies were purified by antigen affinity chromatography using Thermo Scientific AminoLink Plus Coupling Resin according to the manufacturer’s protocol (Thermo Fisher Scientific). The generated antiserum was shown to recognize exclusively the germ cells in testes and early embryos and was used in Western-blot analysis (dilution 1:1000).
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bioRxiv - Immunology 2020Quote: ... Preformed conjugates for antigen-specific B cell sorting were made as described (46) using streptavidin conjugated to PE or AlexaFluor 647 (Life Technologies). B cell probes were made using clade B JR-CSF gp120 ...
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bioRxiv - Immunology 2021Quote: ... SARS-CoV-2 spike protein (Hexapro antigen from Erica Ollmann Saphire, La Jallo for Immunology) was coupled to fluorescent streptavidin beads (Thermo Fisher) and incubated with sera samples to allow antibody binding to occur ...
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bioRxiv - Microbiology 2022Quote: ... 100 μg of antigen plasmid and 320 μl of ExpiFectamineTM CHO reagent were mixed in 7.7 ml of cold OptiPRO™ medium (Thermo Fisher). After the first feed on day 1 ...
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bioRxiv - Microbiology 2022Quote: IgG binding to SARS-CoV-2 or MeV antigens was measured by ELISA using clear flat-bottom immuno nonsterile 96-well plates (Cat# 442404, ThermoFisher Scientific) coated overnight at 4°C with 100 ng of recombinant SARS-CoV-2 proteins or 1 μg of MeV bulk antigen (Cat# BA102VS ...
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bioRxiv - Genomics 2023Quote: ... From this sample a cell clone was derived from primary cells isolated from bat kidney tissue and immortalized via the transfer and stable production of the Simian virus 40 Large T antigen (SV40LT). Kidneys were cut in small pieces (ca. 8 mm3) and incubated in 0.05% Trypsin-EDTA solution (Gibco # 25300-054) for 15 h at 4 ºC ...
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bioRxiv - Microbiology 2023Quote: ... 100 μg of antigen plasmid and 320 μl of ExpiFectamineTM CHO reagent were mixed in 7.7 ml of cold OptiPRO™ medium (Thermo Fisher). After the first feed on day 1 ...
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bioRxiv - Developmental Biology 2023Quote: ... Antigen unmasking was performed by microwaving the slides for 2 × 10 min in citrate buffer solution (pH 6) (Thermo Fisher Scientific). Sections were then blocked for 1 hour at room temperature with 10% normal donkey serum (Chemicon International Inc. ...
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bioRxiv - Immunology 2023Quote: ... and B2(TN99-419) antigens were expressed in FreeStyle 293-F cells by transient transfection in FreeStyle 293 expression media (Thermo Fisher). Cells were co-transfected at a 4:1 ratio of plasmids encoding human metapneumovirus F and furin ...
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bioRxiv - Immunology 2023Quote: ... The monomer form of the antigens was biotinylated and the subsequent tetramerization of these proteins was done with FITC-labeled avidin (Invitrogen, A821), in brief ...
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bioRxiv - Microbiology 2023Quote: ... The antibody-antigen complexes were incubated overnight at 4°C and eluted following Pierce Co-Immunoprecipitation (Co-IP) Kit (Thermo Fisher). Samples were then separated by gel electrophoresis followed by immunoblotting ...
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bioRxiv - Immunology 2024Quote: ... SARS-CoV-2 spike-specific F(ab’)2 was isolated by affinity chromatography using recombinant antigen (1 mg HexaPro) coupled to 0.05 mg dry NHS-activated agarose resin (Thermo Fisher Scientific) as follows ...
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bioRxiv - Microbiology 2024Quote: ... SARS-CoV-2 RBD antigens expressed in 293T cells were used to coat the 96-well ELISA plates (Cat No. 44240421, Thermofisher, USA). 50µL (2µg/mL ...
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bioRxiv - Neuroscience 2023Quote: Low-passage-number Human embryonic kidney cells transformed with SV40 T-antigen (HEK293T) cells were cultured in DMEM (Thermo Fisher Scientific) with 10% fetal bovine serum at 37°C in a humidified atmosphere with 5% CO2 ...
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bioRxiv - Developmental Biology 2024Quote: ... Slides were processed through de-waxing and antigen retrieval in citrate buffer at pH 6.0 using a heat-induced epitope retrieval PT module (Thermo Fisher Scientific) before proceeding with immunostaining ...
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bioRxiv - Biochemistry 2024Quote: ... Recombinant neuraminidase and recombinant hemagglutinin were biotinylated in a 20:1 molar ratio of biotin to antigen with EZ-Link NHS-Biotin (ThermoFisher, 20217) following the manufacturer’s instructions.
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bioRxiv - Microbiology 2024Quote: ... 25 μg F antigen plasmid and 80 μl of ExpiFectamineTM CHO reagent were mixed in 1.9 ml of cold OptiPRO™ medium (Thermo Fisher). After the first feed on day 1 ...
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bioRxiv - Microbiology 2019Quote: ... in the presence of 10 μg of purified Gn or NP antigens in 96 well V-bottomed culture plates (Nunc, Roskilde, Denmark). For intracellular detection of IFN-γ ...
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bioRxiv - Developmental Biology 2019Quote: ... Immunohistochemical analyses were performed on paraffin sections with traditional antigen retrieval and colorimetric development methodologies with the following primary antibodies: anti-Collagen II (Thermo Scientific, MS235B), anti-Collagen X (Quartett ...
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bioRxiv - Neuroscience 2020Quote: HEK293T cells expressing the SV40 large T antigen (HEK293T, ATCC, USA) were cultured in Dulbecco’s modified Eagle’s medium (DMEM, ThermoFisher Scientific, Scoresby, VIC, Australia), supplemented with 10% heat inactivated foetal bovine serum (FBS ...
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bioRxiv - Microbiology 2020Quote: ... Selection of target-binding nanobodies was performed by phage-display selections against immobilized antigen proteins on Maxisorp plates (NUNC, Thermo Fisher Scientific). The initial screening was performed against CS1and CS2 ...
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bioRxiv - Microbiology 2020Quote: ... Selection of target-binding nanobodies was performed by phage-display selections against immobilized antigen proteins on Maxisorp plates (NUNC, Thermo Fisher Scientific). The initial screening was performed against CS1and CS2 ...
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bioRxiv - Microbiology 2020Quote: ELISA was carried out to estimate the avidity and overall stability of antigen-antibody complexes using the chaotropic agent ammonium thiocyanate (NH4SCN) (Acros Organics Fisher Scientific). The plates were coated with 50 ng of PvRBP2b161-1454 in PBS and incubated overnight ...
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bioRxiv - Immunology 2022Quote: Antigen and adjuvant vaccine components were diluted in DPBS (Cytiva, Marlborough, Massachusetts, USA) and then admixed in glass vials (Thermo Fisher Scientific) prior to administration ...
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bioRxiv - Neuroscience 2021Quote: ... all sections were autoclaved for antigen retrieval and immunohistochemical stained with anti-phosphorylated antibodies AT-8 (pSer202/pThr205, MN1020, Invitrogen, 1:250). Immunolabeling was then examined using a DM4000 microscope (Leica ...
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bioRxiv - Physiology 2019Quote: ... Inclusion bodies of the antigen peptides were harvested from the transformant Escherichia coli BL21 Star (DE3) (Thermo Fisher Scientific, Waltham, MA, USA) (37) ...
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bioRxiv - Cell Biology 2021Quote: ... and anti-TRIP4 antibodies were raised in rabbits against affinity purified N-terminally hexahistidine-tagged full-length proteins and purified with the respective antigens coupled to SulfoLink resin (Thermo Fisher Scientific).
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bioRxiv - Immunology 2021Quote: ... Washed yeast libraries were stained in dark and at 4°C with biotinylated antigens pre-conjugated with SA-PE (Streptavidin, R-Phycoerythrin Conjugate, Thermo Fisher Scientific) or SA-APC (Streptavidin ...
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bioRxiv - Immunology 2021Quote: SARS-CoV-2 antigen sequences were cloned into pcDNA3.4 plasmids and transfected into 293F cells utilizing the Expi293 Expression System (Life Technologies, Cat # A14635) per manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... were coated overnight with either Dengue 2 fixed virus antigen (Microbix, Cat# EL-22-02) or polyvalent total immunoglobulin (Invitrogen, Cat# H17000). Before adding PBMC’s ...
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bioRxiv - Immunology 2020Quote: ... the RBD antigen (diluted to 2 μg/ml in PBS) was first adsorbed to 96-well clear Immulon 4 HBX (Thermo Scientific, #3855) plates in PBS overnight at 4 °C ...
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bioRxiv - Immunology 2022Quote: ... A33 and B5 antigens were coupled via carboxyl chemistry to Magplex® fluorescently bar-coded beads (Luminex Corporation) with sulfo-NHS and EDC (Thermo Fisher) per manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... and masked epitopes were recovered with eBioscience™ IHC Antigen Retrieval Solution - High pH (10X) (Invitrogen, Waltham, MA, USA, #00-4956-58). Slides were blocked for 1 hour at room temperature with blocking solution (1xPBS ...
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bioRxiv - Immunology 2023Quote: ... Cells were subsequently stained for viability and surface antigens and then intracellularly stained using the eBioscience Intracellular Fixation and Permeabilization Kit (Thermo Fisher Scientific) using the antibodies and reagents listed in Supplemental Table 5.
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bioRxiv - Pathology 2021Quote: ... for 1 week and pepsin (1 mg/ml in 0.1N HCl) was used for antigen retrieval for immunostaining with human COMP (Thermofisher; MA1-20221, 1:100), CHOP (Santa Cruz ...
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bioRxiv - Molecular Biology 2020Quote: ... a female human embryonic kidney cell line transformed with the SV40 large T antigen was obtained from ATCC (#CRL-1573) and cultured in complete media consisting of DMEM (#11965092, GIBCO, Life Technologies; Carlsbad, CA) supplemented with 10% FBS (Seradigm Premium Grade HI FBS ...
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bioRxiv - Microbiology 2020Quote: ELISA was carried out to estimate the avidity and overall stability of antigen-antibody complexes using the chaotropic agent ammonium thiocyanate (NH4SCN) (Acros Organics Fisher Scientific). The plates were coated with 50 ng of PvRBP2b161-1454 in PBS and incubated overnight ...
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bioRxiv - Biophysics 2022Quote: ... We revealed the antigens coating with a Donkey anti-Goat IgG (H+L) Cross-Adsorbed Secondary Antibody (Alexa Fluor 647, Invitrogen, ref. A-21447) and analyzed the fluorescence intensity ...