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Citations for Thermo Fisher :
151 - 200 of 317 citations for Borrelia Garinii Antigen since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... antigens or capture antibodies were coated in PBS overnight onto 384 microwell plates (Maxisorp, Thermo Fisher Scientific), washed 5 times with PBST (PBS ...
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bioRxiv - Molecular Biology 2020Quote: ... the antigen was detected using chemiluminescence Western blotting detection reagents (Pierce ECL Western Blotting Substrate, Thermo Fisher Scientific Inc. ...
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bioRxiv - Pathology 2022Quote: ... Antigen antibody complexes were visualised with anti-mouse Alexa 488- or 594-conjugated antibody (Thermo Fisher Scientific) or Immpress anti-mouse/rabbit coupled with tyramide amplification kit (PerkinElmer ...
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bioRxiv - Plant Biology 2020Quote: ... The antibody-antigen complex was detected by using the SuperSignal West Pico PLUS Chemiluminescent Substrate (Thermo Scientific) and the FujiFilm LAS-3000 or Amersham imager 600 analyzers.
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bioRxiv - Neuroscience 2019Quote: ... antibody/antigen mixture was incubated with Dynabeads M-280 sheep anti-rabbit IgG (Thermo Fisher Scientific, 11203D) or Dynabeads M-280 sheep anti-mouse IgG (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2020Quote: ... the antigen was detected using chemiluminescence Western blotting detection reagents (Pierce ECL Western Blotting Substrate, Thermo Fisher Scientific Inc. ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... followed by binding of antigen antibody complexes to protein A/G magnetic beads (Thermo scientific, Rockford, USA) for 1 h ...
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bioRxiv - Immunology 2020Quote: ... while non-Avi tagged antigens were biotinylated chemically using EZ-Link Sulfo-NHS-Biotin (Thermo Fisher, A39256). VH and VL sequences of SARS-CoV-2 control antibodies (COV2-2196 ...
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bioRxiv - Cancer Biology 2021Quote: ... epithelial membrane antigen (EMA; clone GP1.4, mouse monoclonal, dilution 1:1000, Thermo Fisher Scientific, Fremont, CA, USA), Sry-related HMG-BOX gene 10 (SOX10 ...
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bioRxiv - Cancer Biology 2020Quote: ... Antigen detection was accomplished with goat-ant-rabbit Cy3 secondary and chicken-anti-mouse Cy5 secondary (ThermoFisher, Waltham ...
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bioRxiv - Immunology 2020Quote: Antigen specific antibodies were depleted in a bead-based approach using Ni-NTA Magnetic beads (Thermo Scientific) as described (62) ...
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bioRxiv - Neuroscience 2022Quote: ... All sections were subjected to heat-mediated antigen retrieval in 10 mM sodium citrate (pH6.5; Fisher Scientific) in distilled water for 20 minutes at 80°C prior to washing 3 times in PBS ...
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bioRxiv - Cancer Biology 2022Quote: ... Antibody/antigen complexes were recovered with Dynabeads M-280 sheep anti-rabbit IgG (Thermo Fisher Scientific, 11203D). Beads with DNA fragments were washed once with RIPA buffer (50 mM Tris-HCl ...
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bioRxiv - Microbiology 2023Quote: ... Protein content of the antigen was determined with Coomassie (Bradford) Protein assay kit (Thermo Fisher Scientific Inc.) according to the manufacturer’s instructions.
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bioRxiv - Immunology 2023Quote: ... and SV40 large T antigen (20 ng) suspended in Opti-MEM I Reduced-Serum Medium (ThermoFisher Scientific), mixed with 293fectin (ThermoFisher Scientific) ...
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bioRxiv - Immunology 2023Quote: ... Protein antigens were coupled to magnetic Luminex beads (Luminex Corp) by carbodiimide-NHS ester-coupling (Thermo Fisher). OSP and LPS antigens were modified by 4-(4,6-dimethoxy[1,3,5]triazin-2-yl)-4-methyl-morpholinium and conjugated to Luminex Magplex carboxylated beads ...
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bioRxiv - Synthetic Biology 2023Quote: All RBD antigens were His-tagged and purified using HisPur Ni-NTA resin (Thermo Fisher Scientific, 88222). Cell supernatants were diluted with 1/3 volume of wash buffer (20 mM imidazole ...
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bioRxiv - Cancer Biology 2024Quote: ... Antigen retrieval was performed by the use of Lab Vision PT Module (Thermo Fisher Scientific, Waltham, MA), in 10 mM citrate pH 6 ...
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bioRxiv - Cancer Biology 2024Quote: ... heat-induced (98°C) antigen retrieval was performed with a citrate buffer (pH 6.0) (Thermo Fisher Scientific), and slides were incubated with Triton X-100 0.25% in PBS for 5 minutes ...
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bioRxiv - Immunology 2020Quote: ... that does not express HLA DR and are Class II major histocompatibility (MHC) antigen negative was maintained in Iscove’s Modified Dulbecco’s Medium (IMDM, Invitrogen) supplemented with 20% FBS ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... and any antigen–MS-Hu6 complex was captured by goat anti–human HRP–conjugated IgG (Invitrogen, Catalog # A18805).
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bioRxiv - Genetics 2020Quote: ... following de-paraffinization the slides were incubated in eBioscience IHC Antigen Retrieval Solution (ThermoFisher; Cat# 00-4955-58) and washed in 1x PBS three times for five minutes ...
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bioRxiv - Cell Biology 2021Quote: ... The antibody-antigen complex was captured by incubating with lysis buffer prewashed proteinA/G beads (Thermo Fisher Scientific) for 2 h ...
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bioRxiv - Biochemistry 2020Quote: ... The antigen-antibody complexes were detected using the Pierce ECL chemi-luminescent detection system (Thermo Scientific, Rockford, IL) and visualized on the ChemiDoc XRS+ gel documentation system (Bio-Rad).
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bioRxiv - Neuroscience 2020Quote: ... The antigen/antibody complex was eluted from beads by boiling in Lane Marker Sample Buffer (#39001, Thermo Fisher) containing β-mercaptoethanol for SDS-PAGE and Western blotting.
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bioRxiv - Microbiology 2019Quote: ... SV40 T-antigens were detected using (pAb)108 and species-specific AlexaFluor 488 (Life Technologies, Thermo Fisher Scientific) antibodies ...
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bioRxiv - Microbiology 2020Quote: ... using antigens conjugated to NHS-activated agarose beads (Pierce™ NHS-Activated Agarose Spin Columns, Thermo Fisher Scientific). Antibodies were eluted in 0.1M glycine ...
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bioRxiv - Neuroscience 2020Quote: ... rabbit or sheep antigens were raised in donkey and conjugated to Alexa 488 or Alexa 594 (Molecular Probes, ThermoFisher ...
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bioRxiv - Bioengineering 2021Quote: ... and subjected to heat- induced antigen retrieval using sodium citrate buffer(10 mM, pH 6, ThermoFisher, Waltham, MA) at >80°C ...
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bioRxiv - Microbiology 2022Quote: Purified F antigens were biotinylated using an EZ-link Sulfo-NHS -LC-Biotinylation kit (Thermo Fisher, cat#A39257) with a 1:1.3 molar ratio of biotin to F ...
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bioRxiv - Microbiology 2019Quote: ... SV40 T-antigens were detected using (pAb)108 and species-specific AlexaFluor 488 (Life Technologies, Thermo Fisher Scientific) antibodies ...
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bioRxiv - Molecular Biology 2021Quote: ... The antiserum was affinity-purified using the peptide antigen coupled to UltraLink™ Iodoacetyl gel (Thermo Fisher Scientific).
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bioRxiv - Developmental Biology 2022Quote: ... after dewaxing and antigen retrieval non-specific antibody binding was minimised by incubating sections in CAS Block (Invitrogen). Primary antibodies were diluted in Dako antibody diluent (S0809 ...
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bioRxiv - Plant Biology 2022Quote: ... Chemiluminescence detection of antigen-antibody complexes was carried out with SuperSignal West Femto Western substrate (Thermo Fisher Scientific). As a loading control ...
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bioRxiv - Neuroscience 2023Quote: ... Antibody/antigen mixture was incubated with Dynabeads M-280 sheep anti-rabbit or mouse IgG (Thermo Fisher Scientific) for 6h at 4°C in the following day ...
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bioRxiv - Biophysics 2023Quote: ... 200 μL of the virus dilution containing 1µg-3µg p24 antigen was transferred to a poly-D-lysine coated glass coverslip (#1 chambered coverglass, Nunc™ Lab-Tek™ ...
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bioRxiv - Neuroscience 2023Quote: ... For antigen retrieval cells were incubated for ten minutes with 1 ug/ml proteinase K (Thermo Fisher Scientific) at room temperature ...
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bioRxiv - Immunology 2023Quote: ... and coronavirus antigens were expressed in Expi293F cells by transient transfection in FreeStyle F17 expression media (Thermo Fisher) supplemented to a final concentration of 0.1% Pluronic Acid F-68 and 20% 4 mM L-glutamine using Expifectamine transfection reagent (Thermo Fisher Scientific ...
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bioRxiv - Synthetic Biology 2023Quote: ... the antigens were captured for 15 mins at 4 °C with 50 µl Streptavidin Magnetic beads (Thermo Fisher) which were previously incubated with superblock (Thermo Fisher ...
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bioRxiv - Cell Biology 2023Quote: ... Target antigens were finally detected using SuperSignal™ West Pico PLUS Chemiluminescent Substrate (Thermo Fisher Scientific, Waltham, MA). Images were scanned and analyzed using ImageJ software ...
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bioRxiv - Immunology 2021Quote: ... SARS-CoV-2 antigens were coupled to magnetic Luminex beads (Luminex Corp) by carbodiimide-NHS ester-coupling (Thermo Fisher). Coupled beads were incubated for 2 hours at 37°C with serum samples (1:10 dilution ...
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bioRxiv - Neuroscience 2020Quote: ... The slides were placed in 250 ml freshly made antigen retrieval solution (0.1 M sodium citrate buffer pH 6.0, Fisher Scientific) and pressure cooked (A ...
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bioRxiv - Immunology 2022Quote: ... The antigen binding fragment (Fab) portion of LY-CoV1404 was generated by proteolytic digestion using immobilized papain (ThermoFisher Scientific), followed by removal of un-cleaved protein using standard chromatography techniques ...
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bioRxiv - Systems Biology 2019Quote: Recombinant protein antigens were first labeled with biotin using EZ-Link™ NHS-LC-Biotin reagent (Thermo Fisher, #21366) and then hybridized with the PETAL array ...
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bioRxiv - Neuroscience 2021Quote: ... Immunoreactivity to the antigen was visualized using Alexa Fluor 488-or Alexa Fluor 594-conjugated secondary antibody (Molecular Probes). Nuclear staining was performed with Hoechst 33258 (Polysciences) ...
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bioRxiv - Microbiology 2020Quote: ... the cells were washed in PBS and incubated for 1-h at RT with 1 mL anti-Early Endosomal Antigen 1 (EEA1) monoclonal antibody (F. 43.1; Invitrogen) diluted 1:200 in blocking buffer ...
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bioRxiv - Neuroscience 2020Quote: ... rabbit or sheep antigens were raised in donkey and conjugated to Alexa 488 or Alexa 594 (Molecular Probes, ThermoFisher, RRIDs ...
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bioRxiv - Immunology 2020Quote: ... Anti-mouse monoclonal antibodies targeting cell surface antigens included anti-GL7 (FITC, PE, eFluor660; clone GL 7; Thermo Fisher), anti-B220 (PE-Cy7 ...
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bioRxiv - Immunology 2022Quote: ... SARS-CoV-2 antigens were coupled to magnetic Luminex beads (Luminex Corp) by carbodiimide-NHS ester-coupling (Thermo Fisher). Coupled beads were incubated for 2 hours at 37°C with serum samples (1:10 dilution ...
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bioRxiv - Immunology 2022Quote: Vaccine 5: The above mentioned hCMP-mRBD antigen when expressed from Stable CHO (Thermo Fisher Scientific, Cat no. R75807) cell lines was named as hCMP-mRBD-CHO ...