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Citations for Thermo Fisher :
51 - 100 of 317 citations for Borrelia Garinii Antigen since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... Heat-induced antigen retrieval (citrate buffer pH 6 or 9 – Thermofisher) was performed using the microwave ...
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bioRxiv - Immunology 2020Quote: ... Antigen–antibody complexes were captured with Protein G-coupled Dynabeads (Invitrogen) and re-suspended in gel loading buffer containing 2% SDS ...
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bioRxiv - Immunology 2020Quote: ... Antigen-specific IgGs were isolated on NHS agarose resin (ThermoFisher; 26196) coupled to the relevant protein ...
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bioRxiv - Cancer Biology 2022Quote: ... 10x low pH antigen retrieval solution (Thermo Scientific, #00-4955-58) was diluted in purified water and preheated for 10 minutes in a microwave ...
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bioRxiv - Neuroscience 2023Quote: Purified antigen was immobilized on a 96 well immunosorbent plate (Nunc). All the following steps were done by gentle shaking on an orbital shaker ...
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bioRxiv - Immunology 2023Quote: ... Antigen specific antibodies reacted to UltraComp eBeads (Invitrogen, 01-3333-42) were used as single-color compensation controls and corresponding isotype controls were included for each antigen specific antibody ...
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bioRxiv - Developmental Biology 2023Quote: ... and boiled in IHC antigen retrieval solution (Invitrogen, #00-4955- 58). Sections were then blocked with 5% goat serum (Vector Laboratories ...
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bioRxiv - Biophysics 2020Quote: ... splenic antigen-presenting cells were washed with RPMI 1640 culture medium (Gibco). Subsequently ...
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bioRxiv - Cell Biology 2019Quote: Antigens were detected using Pierce ECL Western Blotting chemiluminescent substrate (Thermo Fisher), according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... Antigens were mixed with an equal volume of Imject alum adjuvant (Thermofisher) and administered three times ...
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bioRxiv - Bioengineering 2019Quote: ... antigen retrieval was performed using pepsin (Digest-All™ 3, ThermoFisher 003009) for 5 minutes at room temperature ...
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bioRxiv - Cell Biology 2019Quote: ... The antigens were visualized using an ECL substrate kit (Thermo Scientific, USA).
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bioRxiv - Immunology 2019Quote: Monoclonal antibodies specific against the following mouse antigens were purchased from Affymetrix/eBioscience ...
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bioRxiv - Microbiology 2021Quote: ... Plates were coated with MR766 antigen in BupH Carbonate-Bicarbonate buffer (ThermoFisher) and incubated O/N at 4°C ...
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bioRxiv - Cancer Biology 2021Quote: ... The following antibodies against the indicated antigen were purchased from Thermo Fisher Scientific and were used at 1 μg/ml unless stated otherwise ...
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bioRxiv - Immunology 2021Quote: ... The whole viral antigen was coated in the 96 well plate (Nunc) using bicarbonate coating buffer (pH=9.5 ...
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bioRxiv - Molecular Biology 2019Quote: ... Antigens were mixed with an equal volume of Imject alum adjuvant (Thermofisher) and administered three times ...
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bioRxiv - Immunology 2021Quote: ... The following antigens were coupled with carboxylated beads (Luminex Corp): BSA (Affymetrix), goat anti-mouse Igκ ...
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bioRxiv - Immunology 2022Quote: ... Biotinylated antigens were coupled to Neutravidin yellow-green fluorescent beads (Thermo Fisher). Antigen-coupled beads were incubated with 1:500 diluted serum samples for two hours at 37°C ...
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bioRxiv - Immunology 2022Quote: ... The following antigens were coupled with carboxylated beads (Luminex Corp): BSA (Affymetrix), goat anti-mouse Igκ ...
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bioRxiv - Immunology 2023Quote: ... Biotinylated antigen was then added to FITC-conjugated neutravidin beads (ThermoFisher, F8776) at an antigen(μg):bead(μL ...
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bioRxiv - Cancer Biology 2023Quote: ... After treating them with IHC Antigen Retrieval Solution (00-4956-58, Invitrogen), the sections were stained with an anti-human CD4 Ab that was pre-labeled with Alexa Fluor 488 using an Ab labeling kit (Molecular Probes ...
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bioRxiv - Physiology 2023Quote: ... and targeted antigen retrieval performed using boiling 1x Targeted Retrieval Solution (Invitrogen) for 20 mins ...
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bioRxiv - Microbiology 2019Quote: ... and incubated with primary antibody (Rabbit Anti-Group D antigen, Thermo Scientific Singapore) or Guinea Pig Anti-EbpC 45 at 1:500 dilution for 30 minutes ...
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bioRxiv - Biochemistry 2021Quote: ... Antigen-antibody complexes were visualized by applying a streptavidin-biotin complex (Histostain, Invitrogen) for 10 min followed by NovaRED substrate (Vector Laboratories) ...
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bioRxiv - Microbiology 2020Quote: ... Excess antigen was removed by washing three times with Super block (Thermo Scientific) and unbound sites blocked using non-diluted Sea Block ...
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bioRxiv - Pathology 2021Quote: ... After antigen retrieval with mild Proteinase K digestion (50ug/ml Proteinase K (Ambion) for 2min) ...
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bioRxiv - Immunology 2022Quote: ... was coated as antigen (carbonate buffer or DNA-coating reagent from Invitrogen respectively), overnight ...
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bioRxiv - Microbiology 2022Quote: ... Antigen-antibody complexes were selectively purified using Pierce Protein A Agarose (ThermoFisher Scientific) according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2022Quote: ... The antigen-antibody reaction was probed by TMB ELISA Substrate Solution (Invitrogen eBioscience) and was terminated with 1M sulfuric acid after 15 minutes ...
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bioRxiv - Immunology 2019Quote: ... Antigen was immobilized on clear polystyrene 96- or 384-well MaxiSorp plates (ThermoFisher) by overnight incubation at 4 C at 2 ng/uL diluted in phosphate-buffered saline (PBS ...
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bioRxiv - Immunology 2021Quote: ... The following antigens and adjuvants were resuspended in endotoxin-free PBS (Life Technologies) and used for vaccination studies as indicated in figure legends ...
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bioRxiv - Microbiology 2021Quote: ... antigen-antibody complexes were recovered using Pierce magnetic IgA/IgG beads (ThermoFisher Scientific), resolved by gel electrophoresis ...
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bioRxiv - Cancer Biology 2021Quote: ... Antigen was visualized with DAB-Peroxidase Substrate (Thermo Fisher Scientific, Waltham, MA, USA). Finally ...
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bioRxiv - Immunology 2020Quote: ... Expi293F cells for recombinant antigen and monoclonal antibody production (Thermo Fisher Scientific, A1452) were maintained at 37°C in 8% CO2 in Expi293F expression medium (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2021Quote: ... Antigens were coated in a 96 wells plates Nunc-immuno Maxisorp (Thermo Fisher) at 1 μg/mL in carbonate buffer at 4°C overnight ...
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bioRxiv - Cancer Biology 2022Quote: ... Antigen retrieval was performed using Citrate Buffer pH 6.0 (005000, Thermo Fisher Scientific) at 110 °C for 15 minutes ...
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bioRxiv - Biochemistry 2024Quote: ... the viral antigen samples were mixed with 4x LDS loading dye (ThermoFisher Scientific) and 1M DTT ...
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bioRxiv - Bioengineering 2020Quote: ... The antigen-antibody complexes were detected by the ECL system (Thermo Fisher Scientific, #32106). A pre-stained molecular weight marker was run in parallel to determine the molecular weight of the proteins (BioRad ...
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bioRxiv - Neuroscience 2020Quote: ... antigen retrieval was performed using the Lab Vision PT module system (Thermo Scientific, UK), where sections were heated to 100°C for 20 min in citrate buffer (TA-250-PM1X ...
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bioRxiv - Neuroscience 2020Quote: ... antigen retrieval was performed using 1x citrate buffer (pH 6.0,Thermo Fisher, Catalog #005000) at 95°C for 20 minutes followed by incubation in primary antibody solution overnight at 4°C ...
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bioRxiv - Synthetic Biology 2022Quote: All antigens were His-tagged and purified using HisPur™ Ni-NTA resin (ThermoFisher). Cell supernatants were diluted with 1/3rd volume wash buffer [20 mM imidazole ...
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bioRxiv - Cancer Biology 2020Quote: ... Antigen retrieval was performed by steaming in citrate buffer (Thermo Fisher Scientific, Cat# 005000) for 40min ...
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bioRxiv - Immunology 2020Quote: ... Biotinylated antigens were incubated with premium−grade SA−PE and SA−APC (Molecular Probes) or SA-BV421 and SA-BB660 (Biolegend and BD Horizon ...
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bioRxiv - Immunology 2020Quote: ... Confocal related reagents included antigen retrieval buffer low pH (cat. 00-4955-58, Invitrogen), donkey serum (cat ...
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bioRxiv - Immunology 2020Quote: ... Mice were subcutaneously vaccinated with 30 μg OVA antigen in 0.1% LPS alum (Thermofisher) one day after the adoptive transfer of T cells ...
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Intrarenal B cells integrate in situ innate and adaptive immunity in human renal allograft rejectionbioRxiv - Immunology 2020Quote: ... and subjected to antigen retrieval with 10 mM citrate buffer pH 6.0 (ThermoFisher Scientific). Tissue sections were blocked with Tris-buffered saline (TBS ...
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bioRxiv - Immunology 2022Quote: ... antigen was conjugated first with AlexaFluor 647 and AlexaFluor 488 NHS esters (Thermo Fisher) and with BHQ-1 quencher as previously described in(Li et al. ...
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bioRxiv - Immunology 2022Quote: ... The antigen-antibody reaction was visualized by enhanced chemiluminescence assay (ECL, Thermo Fisher Scientific).
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bioRxiv - Microbiology 2022Quote: ... Fecal antigen concentration was determined using Pierce BCA Protein Assay Kit (Thermo Fisher Scientific). Immulon IV ELISA (Nunc ...