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Citations for Thermo Fisher :
3151 - 3200 of 10000+ citations for 2 3 5 6 Tetrahydroxy 4 phosphonooxycyclohexyl dihydrogen phosphate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... sterile Phosphate Buffered Saline (PBS) were purchased from Gibco/Life Technologies Corporation (Carlsbad ...
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bioRxiv - Cell Biology 2024Quote: ... cells were lysed in phosphate buffered saline (PBS, Gibco) with 1% Triton X-100 (Roche ...
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bioRxiv - Genomics 2020Quote: ... The cells were passaged every 2-3 days using 0.25% Trypsin-EDTA (1X) (Gibco, Cat# 25200056) as the dissociation buffer.
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bioRxiv - Microbiology 2020Quote: ... Poly (I:C) and 2’-3’ cGAMP were delivered into cells using Lipofectamine 2000 (Thermo Fisher, USA) as described previously.
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bioRxiv - Biochemistry 2021Quote: ... followed by 2 weeks of selection with 3 μg mL−1 of puromycin (Thermo Fisher Scientific). Then ...
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bioRxiv - Genomics 2021Quote: ... Samples were treated with 2–3 drops of Image-iT FX Signal Enhancer (Molecular Probes; 136933) for 15◻min with rotation at room temperature ...
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bioRxiv - Molecular Biology 2021Quote: ... SNLP mouse fibroblasts and passaged upon ∼80% confluency every 2-3 days using TrypLE Express (ThermoFisher) at room temperature.
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bioRxiv - Systems Biology 2022Quote: ... Peptides were trapped on precolumn (PepMap100 C18 3 μm; 75 μm × 2 cm; Thermo Fisher Scientific) and separated on an EASY-Spray column (ES903 ...
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bioRxiv - Developmental Biology 2024Quote: ... Cell passaging was performed every 2–3 days using 0.25% Trypsin-EDTA (1X) (Gibco, Cat# 25200056) as the dissociation buffer.
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bioRxiv - Genetics 2024Quote: Testes from 2–3 day-old flies were dissected and transferred to Schneider’s insect medium (Gibco) with or without 50 ng/µl α-amanitin ...
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bioRxiv - Cell Biology 2023Quote: ... All cells were passaged every 2-3 days using 0.05% Trypsin/EDTA (Gibco, Catalog no. 25300054). All cell lines were incubated at 37°C and maintained at 5% CO2.
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bioRxiv - Molecular Biology 2023Quote: ... fed daily with growth factors and passaged every 2-3 days using TrypLE Express enzyme (GIBCO) to detach cells and trypsin inhibitor (GIBCO ...
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bioRxiv - Immunology 2023Quote: ... The tissue was then cut into 2-3-cm segments and incubated in RPMI media (ThermoFisher) containing 5 mM EDTA ...
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bioRxiv - Neuroscience 2023Quote: ... 11319718) were coated dropwise with 1,1′-Dioctadecyl-3,3,3′,3′-Tetramethylindocarbocyanine Perchlorate (DiI, 2 mg, Invitrogen, D282) dissolved in dichloromethane (400 ul ...
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bioRxiv - Immunology 2023Quote: ... Peptides were trapped on precolumn (PepMap100 C18 3 μm; 75 μm × 2 cm; Thermo Fisher Scientific) and separated on an EASY-Spray column (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2023Quote: ... 3 and 24 hours later the plating medium was exchanged against NeurobasalTM containing 2% BL27 (Gibco) and 0,8 mM L-glutamine (all Gibco) ...
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bioRxiv - Cell Biology 2023Quote: ... Coverslips were prepared by incubating with a 2% solution of (3-aminopropyl)trimethyoxysilane (313255000, Acros Organics) diluted in isopropanol ...
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bioRxiv - Bioengineering 2024Quote: ... and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT, 0.5mg/ml, #V13154, Thermo Fisher) for 1 h ...
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bioRxiv - Cell Biology 2024Quote: Sodium salt of 3-methyl-2-oxobutanoic acid (KIV; cat # AC189720050) was purchased from Thermo Fisher Scientific (Waltham ...
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bioRxiv - Physiology 2024Quote: ... 3-month and 2-year tissue samples were quantified using a BCA Assay (Thermo Scientific VLBL00GD2). Equal amounts of proteins were run on 4%–20% Tris-glycine gels (Invitrogen WXP42012BOX) ...
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bioRxiv - Immunology 2024Quote: ... for 3 hours followed by one-hour treatment of 2 mM ATP (Thermo Fisher Scientific, R0441). To determine how efferocytosis affect caspase-1 cleavage ...
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bioRxiv - Immunology 2024Quote: ... cells were diluted 1:3 in PBS with 2 mM EDTA and 1:1000 DAPI (ThermoFisher). Cells were counted using Forward Scatter (FSC ...
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bioRxiv - Bioengineering 2024Quote: ... and 2 days later cells were detached using 3 ml of TrypLE (Thermo Fisher, CAT: 12604021). Cells were moved to a non-tissue culture treated 6-well plate (VWR ...
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bioRxiv - Cell Biology 2020Quote: The 5’ and 3’ ends of lncRAP2 was determined using the FirstChoice RLM-Race Kit from Ambion following the manufacturers instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... HCT116 cells complemented with chromosomes 3 and 5 were cultured with 400 μg/mL geneticin (G418, Gibco) and 6 ug/mL blasticidine (Invivogen) ...
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bioRxiv - Molecular Biology 2021Quote: ... Staining of cells was performed using 5 μl of DiBAC4(3) (Invitrogen, 0.025 mg/ml in DMSO) followed by incubation for 15 minutes at room temperature in dark ...
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bioRxiv - Cancer Biology 2020Quote: ... 5’-CAGGAAACAGCTATGAC-3’) and was subsequently recombined with the destination vector using LR Clonase (Thermo Fisher Scientific). The plasmids were then transformed in Agrobacterium tumfaciens strain GV3101.
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bioRxiv - Cell Biology 2021Quote: ... Sections were washed 3-5 times with TBSTX buffer and then mounted on Superfrost slides (Fisher Scientific) with Slow fade Diamond mounting solution (Invitrogen ...
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bioRxiv - Cell Biology 2021Quote: ... Mouse ENKD1 shRNA (nucleotides targeting 403-423 bp, 5′-CGCTCACCCAAGTATGACAAT-3′) were cloned into pLKO.1 (Invitrogen).
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bioRxiv - Molecular Biology 2020Quote: ... while the EASY-Spray C18 column (Thermo Scientific, 15 cm×75 μm ID, 3 μm, 5 μm) was used as the analytical column ...
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bioRxiv - Cancer Biology 2020Quote: ... 5’-\56-FAM\ CCT ACC TTA ACC TCC C-3’ with Minor Groove Binder (MGB; ThermoFisher Scientific). PCR was performed using 10X Master Mix to yield a final volume of 25 µl and final working concentrations of 16.6mM (NH4)2SO4 ...
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bioRxiv - Cell Biology 2020Quote: ... Sections were thawed at room temperature and washed 3 times 5 minutes in DPBS (Gibco, 14190-094). Sections were then counterstained for 5 minutes with Hoechst 33342 (1μg/mL ...
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bioRxiv - Neuroscience 2022Quote: ... 5′-CCCAAGACTTCCACCTACATTC -3′ (Tm = 62°C) and subsequently subcloned into PCR II-TOPO-TA cloning vector (Invitrogen). Riboprobes were generated with a BIOT-NTP labeling kit (Roche) ...
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bioRxiv - Microbiology 2022Quote: ... Primers used to amplify the 5’/3’ junction with Phusion high-fidelity DNA polymerase (Thermo Fisher Scientific) are listed in Table S3 ...
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bioRxiv - Microbiology 2022Quote: ... Data analysis was performed with the Quantstudio 3&5 Design and analysis software (version v1.5.1, Applied Biosystems). Cq values were obtained via automated threshold determination ...
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bioRxiv - Immunology 2022Quote: ... resuspended in pre-sort buffer containing 3-5 nM SYTOX Green Nucleic Acid Stain (Thermo Fisher Scientific), and incubated for 20 min at 4°C ...
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bioRxiv - Biophysics 2021Quote: ... trypsinized for 3 mins with 5 ml of an EDTA/Trypsin solution (0.05 %, 59417C, Gibco, Thermo Fisher), and diluted with low glucose DMEM (Dulbecco’s modified Eagle’s medium ...
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bioRxiv - Microbiology 2020Quote: ... flanked by 5’ Nhel/3’Apal sites for subcloning into the pcDNA3.1(+) vector (Thermo Fisher Scientific, USA). HEK293T cells and Vero E6 cells were purchased from ATCC ...
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bioRxiv - Cell Biology 2022Quote: ... 2.5 mM probenecid (Life-Technologies: catalog # P36400) and 5 µM Fluo-3 (Life Technologies; catalog # F-14218). Cells were incubated at 37°C for 30 minutes before being centrifuged and resuspended in modified L-15 media containing 2% FBS and 2.5 mM probenecid ...
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bioRxiv - Immunology 2020Quote: ... containing a 5′-CACC-3′ sequence required for directional TOPO cloning in pENTR/D-TOPO (Invitrogen, USA), and a reverse primer [“Tgd057 R HA stop” 5’-TTACGCGTAGTCCGGGACGTCGTACGGGTACTCGACCTCAATGTTGTATTC-3’] ...
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bioRxiv - Microbiology 2021Quote: ... for 3 hours at 37 °C before transforming 5 μL into TOP 10 chemically-competent bacteria (Invitrogen). Mutations were confirmed in selected clones by DNA sequencing ...
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bioRxiv - Genomics 2020Quote: ... (RV-5′-GCCGTGGGTACTGAGAAAG-3′) and the fragments were bi-directional sequenced using (3130 Genetic Analyser; Applied Biosystems). Further the results were compared with the reference sequence of ARHGEF40 gene using NCBI-BLAST program and the chromatogram was analysed using Chromas lite (2.1 ...
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bioRxiv - Biophysics 2021Quote: ... trypsinized for 3 mins with 5 ml of an EDTA/Trypsin solution (0.05 %, 59417C, Gibco, Thermo Fisher), and diluted with low glucose DMEM (Dulbecco’s modified Eagle’s medium ...
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bioRxiv - Immunology 2022Quote: ... Probe: 5’-FAM-ACC CCG CAT TAC GTT TGG TGG ACC-NFQ-MGB-3’ (Thermo Fisher Scientific).
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bioRxiv - Biophysics 2022Quote: ... Sections were washed 3× for 5 min each in PB and mounted with Prolong Gold (Thermo Fisher) and Deckglaser cover glass (Cat#NC1776158) ...
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bioRxiv - Cell Biology 2022Quote: ... 5′-RACE and 3′-RACE were done using the GeneRacer method according to the manufacturer’s instructions (Invitrogen). Briefly ...
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bioRxiv - Immunology 2022Quote: ... cDNA was amplified using ISPCR primer (5′-AAGCAGTGGTATCAACGCAGAGT-3′) and KAPA HiFi HotStart ReadyMix (Thermo Fisher Scientific), cleaned up using RNAClean XP SPRI beads three times ...
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bioRxiv - Microbiology 2024Quote: ... Membranes were washed 3 x 5 min with TBST and developed using chemiluminescent substrate (ThermoFisher SuperSignal West) and imaged using a ChemiDoc (Bio-Rad) ...
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bioRxiv - Cell Biology 2023Quote: ... At 3 hours post infection (hpi) cells were seeded on 5 mm round glass coverslips (Thermo Scientific) and mounted on a sample holder specially designed for LLSM ...
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bioRxiv - Neuroscience 2023Quote: ... 5’-CAGAGTCGATCAGTCTGCATATCTCCA-3’) with the dilution protocol of the Phusion Human Specimen Direct PCR Kit (Thermo Scientific). The PCR product was sequenced directly with a nested sequencing primer (5’-GGTGCTCTCCCGGGTACACAA-3’) ...