Labshake search
Citations for Thermo Fisher :
2851 - 2900 of 10000+ citations for IL 1beta Mouse CHO since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Synthetic Biology 2020Quote: 90 μL CFE semi-continuous reactions were performed using 3.5 kDa MWCO 96-well plate dialysis cassettes (Thermo Scientific, Rockford, IL, USA) in 2 mL microcentrifuge tubes with 1.4 mL dialysis buffer solution ...
-
bioRxiv - Immunology 2019Quote: ... a 20X IL-26 human TaqMan® probe was used (Hs00218189_m1) with 2X TaqMan® Fast Advanced Master Mix (both from ThermoFisher Scientific). OSM and HEBGF cDNA quantification was performed with Roche ® hydrolysis probes (OSM ...
-
bioRxiv - Cancer Biology 2020Quote: ... The cells were cultured at 37 °C, 5% CO2 in RPMI medium 1640 (Pierce, Rockford, IL) with 10% dialyzed fetal bovine serum (Invitrogen, Carlsbad, CA) and 1% penicillin/streptomycin for at least five passages to completely label the proteome with stable isotope labeled heavy Arg and Lys for three-state SILAC experiments ...
-
bioRxiv - Cell Biology 2019Quote: ... and then incubated in PBS plus 0.5% Triton X-100 and Alexa Fluor 568-conjugated Phalloidin at 0.005 units/μl (Molecular Probes/ Invitrogen, Rockford, IL) at room temperature for 1 h ...
-
bioRxiv - Immunology 2019Quote: ... purified ILC3 were cultured for 16 hours in the above conditions and the supernatant analysed using the IL-22 ELISA Kit (Thermo Fisher Scientific).
-
bioRxiv - Physiology 2021Quote: ... The total concentration of proteins was measured with the Pierce Rapid Gold BCA Protein assay kit (Thermo Fisher Scientific, Rockford, IL, USA). The E- ...
-
bioRxiv - Neuroscience 2021Quote: ... at room temperature for 5 min and then the slides were mounted using Fluoromount(tm) (Life Technologies-Thermo scientific, Rockford, IL, USA) as a mounting medium.
-
bioRxiv - Developmental Biology 2021Quote: ... and frozen overnight at -80 °C in a NALGENE Cryo 1 °C freezing container (5100-0001, ThermoFisher Scientific, Hanover Park, IL, USA) filled with isopropanol (423830025 ...
-
bioRxiv - Developmental Biology 2021Quote: ... and 75% methanol in 1x PBS and three times in 100% methanol (A412-4, Fisher Chemical, Fisher Scientific, Hanover Park, IL, USA). Samples were stored at -20 °C in 100% methanol until processing.
-
bioRxiv - Microbiology 2020Quote: ... Japan were cultured at 37 °C in Dulbecco’s modified Eagle’s medium (DMEM, Nacalai Tesque, Japan) supplemented with 5% heat-inactivated fetal bovine serum (FBS, Thermo Fisher Scientific, Rockford, IL) and antibiotics (10 units/mL penicillin and 10 μg/mL streptomycin ...
-
bioRxiv - Genetics 2019Quote: ... The ovarian tissues were homogenized with M-PER Mammalian Protein Extraction Reagent (Pierce Biotechnology, Rockford, IL, USA) supplemented with Halt TM Protease Inhibitor Cocktail (Thermo Fisher Scientific). Following centrifugation (16,000 g ...
-
bioRxiv - Developmental Biology 2022Quote: ... They were then washed briefly with PST and incubated in secondary antibodies and Hoechst 33342 (1:100,000; Thermo Fisher, Rockford, IL, USA) for DNA staining overnight at room temperature ...
-
bioRxiv - Immunology 2022Quote: ... with anti-His and anti-IL-15 antibodies and live/dead staining) using the LIVE/DEAD Fixable NearIR Dead cell stain kit (Thermo Fisher Scientific) for all flow cytometry analysis ...
-
bioRxiv - Immunology 2022Quote: ... then assayed in technical duplicate for secreted chemokines and cytokines by ELISA (IL-15, BAFF, CXCL10/IP-10, and CXCL11/I-TAC, all R&D Systems; CXCL9/MIG, Invitrogen) according to the manufacturer’s protocol ...
-
bioRxiv - Microbiology 2022Quote: ... They were then incubated with primary antibody rabbit polyclonal anti-claudin-1 (Catalog# 51-9000; RRID: AB_2533916; final concentration, 1:100; Invitrogen, Rockford, IL, USA) overnight in a humid chamber at 4°C ...
-
bioRxiv - Physiology 2023Quote: ... Total IL-1β protein level was measured by mouse IL-1β/IL-1F2 DuoSet ELISA kit and first normalized to total protein level quantified by Pierce™ BCA Protein Assay Kit (Thermo Scientific), and then normalized to the sham group.
-
bioRxiv - Microbiology 2022Quote: ... and intracellular markers were stained with monoclonal antibodies for 45 minutes at 4°C with FoxP3 (FJK-16S) and IL-10 (JES5-16E3) (All antibodies were from Thermo Fisher Scientific). Cells were resuspended in PBS with 2% NCS and 2 mM EDTA ...
-
bioRxiv - Immunology 2022Quote: ... cell supernatants from DC or DC-T cell co-cultures were collected and measured using a commercially available kit for IL-10 (Thermo Scientific/eBiosciences), IFNβ (PBL Assay Science) ...
-
bioRxiv - Physiology 2022Quote: ... MA) or 1 mM caffeine were used to evoke Ca2+ transients from fluo-4AM or rhod-2AM (Thermo Scientific, Rockford, IL, USA) loaded myocytes ...
-
bioRxiv - Cell Biology 2022Quote: ... Cells were then washed with PBS lacking calcium and magnesium and lysed with IP Lysis buffer (Thermo Fisher Scientific, Rockford, IL, USA) supplemented with protease inhibitor cocktail kit (Thermo Fisher Scientific ...
-
bioRxiv - Molecular Biology 2024Quote: ... were isolated from mice tibias and femurs as described previously29 and cultured for 7 days in Iscove’s Modified Dulbecco’s Medium supplemented with 10% FCS (Thermo Scientific, Rockford, IL, USA), penicillin-streptomycin (100 U/mL and 100 μg/mL ...
-
bioRxiv - Molecular Biology 2024Quote: ... Cat#CL-0190) and cultured in Dulbecco’s Modified Eagle’s Medium supplemented with 10% fetal bovine serum (FBS) (Thermo Scientific, Rockford, IL, USA), 100 U/mL penicillin (Solarbio ...
-
bioRxiv - Cell Biology 2024Quote: Primary or secondary antibodies were purchased from the following vendors: rabbit anti-human ROBO1 (PA5-29917, Thermo Fisher Scientific, Rockford, IL, USA); rabbit anti-human ROBO1 (HPA052968 ...
-
bioRxiv - Immunology 2024Quote: ... SuperSignal™ West Femto Maximum Sensitivity Substrate and Halt™ Protease and Phosphatase Inhibitor Cocktail were purchased from Thermo Scientific (Rockford, IL). Chemical inhibitors ...
-
bioRxiv - Pathology 2023Quote: ... The endotoxin content in the silica particles was <1.0 pg/μg of silica as determined using the LAL Chromogenic Endotoxin Quantitation Kit (Thermo Scientific, Rockford, IL) according to the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2023Quote: Levels of IFN-λ in the culture supernatants were determined with ELISA using the human IL-29 ELISA kit (Invitrogen, cat. BMS2049) following the instructions of the manufacturers ...
-
bioRxiv - Physiology 2023Quote: ... Samples were then washed in PBS (3 x 5 minutes in PBS at room temperature) and mounted in ProLong Gold (Invitrogen, Rockford, IL). For STochastic Optical Reconstruction Microscopy (STORM) ...
-
bioRxiv - Evolutionary Biology 2023Quote: Pellets were thawed on ice and resuspended in 20 mL of HisTrap Binding Buffer (50 mM NaH2PO4, pH 7.4, 300 mM NaCl, 10 mM imidazole) and 2 µL Pierce Universal Nuclease (Thermo Scientific, Rockford, IL). Once resuspended ...
-
bioRxiv - Immunology 2023Quote: ... CTLA-4 or PD-1) were suspended in IL-2 medium at 37°C containing either 65nM LysoTracker® Deep Red (ThermoFisher Scientific) or 25nM MitoTracker™ Red CMXRos for 1h or 30min ...
-
bioRxiv - Bioengineering 2022Quote: ... Dam serum and placental tissue lysates (both prepared as described above) were assayed for IL-6 concentration using an ELISA per manufacturer instructions (Invitrogen, Waltham, MA). Sample absorbance values were compared to a standard curve to calculate IL-6 concentration.
-
bioRxiv - Cell Biology 2023Quote: ... Lysates were clarified by centrifugation at 17,000 xg for 5 min and total protein was quantified in the supernatant using the BCA protein assay (Thermo Scientific, Rockford, IL). SDS loading buffer (final concentrations of 100 mM Tris pH 6.8 ...
-
bioRxiv - Immunology 2023Quote: Ex vivo isolated and rested CD8+ T cells or transduced T cells from splenocytes were incubated with R10 (RPMI 1640 + 10% FBS + 1% penicillin/streptomycin + L-glutamine + 20 U/ml of IL-2; Gibco, Thermo Fisher Scientific) alone or R10 containing PMA/ionomycin for two hours ...
-
bioRxiv - Immunology 2023Quote: ... Quantitative analysis of IL-6 and IFNB expression in THP1 dual reporter cells was performed by StepOne Real-Time PCR Systems (Thermo Fisher Scientific) by using 18S rRNA for normalization ...
-
bioRxiv - Cell Biology 2023Quote: ... and C-X-C motif chemokine 11 (CXCL11) ELISAs were performed using basal supernatants collected 48 hours after cytokine treatments using IL-8 Human ELISA Kit (ThermoFisher, Cat# KHC0081) and Human CXCL11/I-TAC Quantikine ELISA Kit (R&D Systems ...
-
bioRxiv - Molecular Biology 2020Quote: ... INS1 cells labeled with Bodipy C12 were also immunostained with mouse anti-HSP60 antibody (66-41-1-lg, Protein Tech) and Alexa Fluor 488 goat anti-Mouse IgG (A11017, Invitrogen) both at 1:300 ...
-
bioRxiv - Molecular Biology 2020Quote: ... 5’TYE563-labeled locked nucleic acid probes (Exiqon) against mouse major satellite sequences (Supplementary Table 5) were precipitated with mouse Cot-1 DNA (Invitrogen) and Salmon Sperm DNA (Thermo Fisher Scientific) ...
-
bioRxiv - Molecular Biology 2021Quote: ... Blots were developed with a mouse polyclonal anti-SmHDAC8 antibody (1/1000) or mouse polyclonal anti-SmRho1 antibody (1/1000) and peroxidase coupled anti-mouse secondary antibody (1/50 000; Invitrogen). Detection was carried out by chemiluminescence using SuperSignal™ West Dura Extended Duration Substrate (Thermo Scientific ...
-
Neuronal L-Type Calcium Channel Signaling to the Nucleus Requires a Novel CaMKIIα-Shank3 InteractionbioRxiv - Neuroscience 2019Quote: Mouse forebrains fractions or HEK293T cell lysates were incubated with either mouse anti-CaMKIIα (Thermo Fisher Scientific catalog #MA1–048), rabbit anti-Shank3 (Bethyl catalog #A304-178A) ...
-
bioRxiv - Cell Biology 2021Quote: ... mouse monoclonal anti-Mcm3 antibody (M038-3; MBL) and anti-mouse IgG conjugated to Dynabeads M-280 (Thermo Fisher Scientific) were used as the primary and secondary antibodies ...
-
bioRxiv - Cancer Biology 2020Quote: ... Wells were consecutively washed two times for 5 min in washing buffer before incubation with secondary antibodies (Alexa Fluor-488 goat anti-mouse IgG, Invitrogen, A-11029; Alexa Fluor-647 goat anti-mouse IgG, Invitrogen, A-21236 ...
-
bioRxiv - Cell Biology 2021Quote: ... Alexa-Fluor-488-conjugated goat anti-mouse-IgG antibodies and Alexa-Fluor-594-conjugated goat anti-mouse-IgG antibodies (Thermofisher) were used.
-
bioRxiv - Microbiology 2020Quote: ... Samples were separated by SDS-PAGE and Western blotting carried out using a mouse mono- and polyubiquitinated ubiquitin conjugate (Ubiquigent) or mouse Ub-K63 (ThermoFisher) antibody with HRP-conjugated anti-mouse secondary antibody (GE Healthcare or Promega) ...
-
bioRxiv - Microbiology 2020Quote: ... Mouse monoclonal antibodies against binary toxin subunits CDTa and CDTb and anti-Clostridia GDH monoclonal mouse antibody (Clone: J44D, Invitrogen) were obtained from Merck ...
-
bioRxiv - Cell Biology 2021Quote: ... Cells were then incubated for 1 hr at room temperature in the dark with secondary antibodies Alexa488-conjugated goat anti-mouse IgG and Alexa546-conjugated goat anti-mouse IgG (Invitrogen). Nuclei were stained with DAPI (Sigma-Aldrich) ...
-
bioRxiv - Bioengineering 2022Quote: ... Additional labeling of C2C12 with MF-20 hybridoma mouse IgG2B primary antibody and Alexa Fluor 546 goat anti-mouse IgG2B secondary antibody (Invitrogen) was used to evaluate the cell differentiation into myotubes ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: ... the anti-BiP mouse monoclonal antibody and the anti-mouse secondary antibody were obtained from Invitrogen (Grand Island, NE, USA). Dimethyl sulfoxide (DMSO ...
-
bioRxiv - Microbiology 2022Quote: Cytokines in mouse lung homogenates were measured using a custom ProcartaPlexTM Mouse Mix & Match Panels kit (Invitrogen Waltham, MA, USA) on the Bio-Plex 200 (Bio-Rad Laboratories Ltd).
-
bioRxiv - Microbiology 2021Quote: ... incubated with corresponding secondary antibodies (Alexa488- and 594-conjugated anti-rabbit IgG, anti-mouse IgG, or anti-mouse IgG1 (1:500; Invitrogen)) in blocking buffer for 2 hours at room temperature ...
-
bioRxiv - Microbiology 2021Quote: ... incubated with corresponding secondary antibodies (Alexa 488-/ 594-conjugated anti-rabbit IgG, anti-mouse IgG, or anti-mouse IgG1 (1:500; Invitrogen)) in blocking buffer for 2 hours at room temperature ...
-
bioRxiv - Molecular Biology 2021Quote: ... Binding of monomeric VHHs to SARS-CoV-1 or SARS-CoV-2 S was detected with a mouse anti-HisTag antibody (AbD Serotec) and an AF647 conjugated donkey anti-mouse IgG antibody (Invitrogen). Following 3 washes with PBS containing 0.5% BSA ...