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Citations for Thermo Fisher :
2751 - 2800 of 10000+ citations for IL 1beta Mouse CHO since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... RNA polymerase subunit β was used as a loading control and detected using anti-RNAP antibody (Thermo fisher, Rockford, IL, USA). Densitometry was performed using the ImageJ software (71 ...
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bioRxiv - Microbiology 2021Quote: ... Activation of CD4+ T cells was achieved by addition of 25 U/ml of interleukin-2 (IL-2) and CD4+ T-cell activation Dynabeads (Life Technologies). CD4+ T-cells activated for 4-5 days were used for HIV-1 infections ...
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bioRxiv - Microbiology 2020Quote: ... The membranes that were sequentially probed with more than one antibody were stripped in stripping buffer (Thermo Scientific, Rockford, IL, USA) before re-probing.
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bioRxiv - Cancer Biology 2021Quote: ... a drop of prolong gold anti-fade reagent with DAPi was added to each section (Thermo Fisher Scientific, Rockford, IL, USA), and slides were mounted and sealed with nail polish ...
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bioRxiv - Cancer Biology 2021Quote: ... A drop of prolong gold anti-fade reagent with DAPi was added to each section (Thermo Fisher Scientific, Rockford, IL, USA), and slides were mounted and sealed with nail polish ...
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bioRxiv - Biochemistry 2022Quote: ... The soluble supernatant was collected and protein concentration was quantified via bicinchoninic assay (BCA) assay (Pierce Thermo Scientific, Rockford, IL, USA). Normalized soluble supernatant or 20 μL of conditioned media was run on a 4-20% Tris-Gly SDS-PAGE gel (Life Technologies ...
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bioRxiv - Immunology 2022Quote: ... Cells were incubated in RP-10 supplemented with 10 ng/ml recombinant human IL-2 and 2 μg/ml anti CD28 (clone 37.51, Thermo Fisher Scientific) for 18-22 hours ...
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bioRxiv - Microbiology 2022Quote: ... Primary human CD4+ T cells stimulated with IL-2 alone or with α-CD3/CD28 antibody-coated beads (Gibco/Thermo Fisher) were infected for 5 days ...
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bioRxiv - Microbiology 2022Quote: ... Primary human CD4+ T cells stimulated with IL-2 alone or with α-CD3/CD28 antibody-coated beads (Gibco/Thermo Fisher) were infected for 5 days ...
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bioRxiv - Neuroscience 2022Quote: ... the protein concentration in the supernatant was determined with a BCA Protein Assay kit (Pierce, Thermo Fisher Scientific, Rockford, IL, USA). To each protein sample (20 μg) ...
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bioRxiv - Microbiology 2022Quote: ... IP control antibody included IgG purified from normal rabbit serum with Melon Gel IgG Spin Purification Kit (Thermo Scientific, Rockford, IL). The Co-IPs performed in triplicate.
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bioRxiv - Neuroscience 2020Quote: ... These lysates were then heated to 94°C for 10 minutes to denature the proteinase K (Thermo Scientific, Rockford, IL, USA), and centrifuged at 16,100g for 15 minutes ...
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bioRxiv - Physiology 2021Quote: ... protein content was measured from 100 μl of diluted samples with Pierce™ BCA Protein Assay Kit (ThermoFisher, Rockford, IL, USA) and lipid content was analyzed by hydrolysis according to an internal method adapted from NF ISO 6492 by Artemis Laboratory (Janzé ...
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bioRxiv - Physiology 2021Quote: ... The supernatant was collected and protein concentration was quantified via a bicinchoninic assay (BCA) assay (Pierce Thermo Scientific, Rockford, IL, USA). Approximately 30 μg of soluble supernatant was run on a 4-20% Tris-Gly SDS PAGE gel (Life Technologies ...
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bioRxiv - Neuroscience 2021Quote: ... PS2 or PSD fractions were quantified for protein concentration using the Qubit® Protein Assay Kit (Thermo Scientific Rockford, IL, USA).
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bioRxiv - Cancer Biology 2021Quote: ... supplemented with 10% fetal bovine serum (FBS)) and 30IU/mL recombinant human IL-2 (rhIL-2; Thermo Fisher Scientific, Waltham, MA).
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bioRxiv - Biochemistry 2021Quote: ... Two μg of total RNA were subjected to reverse transcription using High-Capacity cDNA Reverse Transcription Kit (Thermo Scientific, Rockford, IL) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... IL-1-Ra plasma levels were determined of 20 patients positive and 12 patients negative for IL-1-Ra-Abs with a commercially available ELISA kit (Invitrogen/ThermoFisher #BMS2080) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: CVL supernatants were recovered by centrifugation for assessment of IL-8 levels by Enzyme-Linked Immunosorbent Assay (ELISA) (Invitrogen, Paisley, UK), following manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... and supplemented with 100 U/mL of penicillin G and 100 μg/mL of streptomycin (SV30010; Thermo Scientific, Rockford, IL, USA) at 37 °C in a humid atmosphere of 5% CO2 ...
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bioRxiv - Immunology 2022Quote: ... and (2) co-cultured with KPC CAFs with or without concurrent treatment with anti-IL-1β neutralizing antibody (Thermofisher, Waltham, MA) and IL-1R1 inhibitor Anakinra (SOBI Pharmaceuticals ...
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bioRxiv - Cancer Biology 2022Quote: ... whole-cell protein extracts from harvested splenocytes were prepared using RIPA buffer (ThermoScientific, Rockford, IL) containing a protease/phosphatase inhibitor cocktail (Thermo Scientific). Protein quantification was performed using the Pierce BCA protein assay kit (ThermoScientific ...
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bioRxiv - Immunology 2019Quote: ... 100 μg/mL streptomycin with either 110 U/mL granulocyte-macrophage colony-stimulating factor (GM-CSF) (Leukine; Sanofi) and 282 U/mL interleukin-4 (IL-4) (Affymetrix, eBioscience) for DCs or 280 U/mL GM-CSF for macrophages ...
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bioRxiv - Biochemistry 2020Quote: Protein concentration in the cell extract was measured using a Pierce BCA protein assay kit (product 23227; Thermo Scientific, Rockford, IL).
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bioRxiv - Synthetic Biology 2020Quote: ... Dialysis was performed using Slide-A-Lyzer™ Dialysis Cassettes with a 3.5 kDa cut-off (Thermo Scientific, Rockford, IL, USA). Extracts were dialyzed three times with 150 mL S30 buffer per mL extract for 45 min at 4 °C ...
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bioRxiv - Microbiology 2019Quote: ... ELISAs were conducted following the manufacturers’ protocols, with the IL-1b and IFN-y (ELISAKIT.com, EK-0033 and EK-0002, respectively) and TNF-a (ThermoFisher Scientific KMC4022). Samples were diluted 1 in 5 and 1 in 10 for IL-1b ...
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bioRxiv - Biochemistry 2021Quote: TE proteins were fluorescently labeled with the amine-reactive dye Alexa Fluor 647 NHS-Ester (Thermo Fisher Scientific, Rockford, IL, USA) as follows ...
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bioRxiv - Cell Biology 2019Quote: ... and then incubated in PBS plus 0.5% Triton X-100 and Alexa Fluor 568-conjugated Phalloidin at 0.005 units/μl (Molecular Probes/ Invitrogen, Rockford, IL) at room temperature for 1 h ...
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bioRxiv - Immunology 2020Quote: ... Murine IFNγ and IL-2 cytokines were measured in supernatants according to the Murine IFNγ and IL-2 ELISA Ready-SET-GO!® ELISA kit (Invitrogen) according to manufacturer’s protocol ...
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bioRxiv - Plant Biology 2021Quote: ... Tissue was immediately placed into lysis bags provided in the P-PER Plant Protein Extraction Kit (Thermo Scientific, #89803, Rockford, IL) and lysed according to provided instructions with the following modification ...
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bioRxiv - Immunology 2020Quote: ... The isolated CD8+ T cells were washed and resuspended at a concentration of 1 × 106 cells per ml in completely reconstituted RPMI supplemented with 50 units/ml IL-2 and 1 × 106 CD3/CD28-coated Human T-Activator Dynabeads (Life Technologies) per ml ...
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bioRxiv - Immunology 2020Quote: ... by negative selection magnetic bead sorting (Miltenyi) and activated in complete RPMI supplemented with 30 U/ml IL-2 with anti-human CD3/CD28 dynabeads (Thermo Fisher) at a 1:1 cell-to-bead ratio.
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bioRxiv - Developmental Biology 2021Quote: ... whole mount duck and quail embryos were stained with Hoechst 33342 Dye (62249, Lot# RF22228410, ThermoFisher Scientific, Hanover Park, IL, USA). Briefly ...
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bioRxiv - Developmental Biology 2021Quote: ... Cells were then stored at -140 °C in liquid nitrogen (CryoPlus Storage Systems model 7402, ThermoFisher Scientific, Hanover Park, IL, USA).
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bioRxiv - Cell Biology 2020Quote: ... The concentration of protein crude extract was determined by means of bicinchoninic acid (BCA) protein assay (Thermofisher Scientific, Rockford, IL, USA).
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bioRxiv - Immunology 2021Quote: Purified CD8+ cells were cultured in AIMV medium supplemented with 10% FBS and 100 U/ml recombinant human IL-2 (ThermoFisher Scientific) and used for sorting of CD8+ T cell subsets and for further experiments three days after isolation.
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bioRxiv - Immunology 2020Quote: ... The MMP Luminex panel (PPX-05/ PROCARTAPLEX MMP1, MMP2, MMP3, MMP9 and MMP13 plex) and IL-6 Elisa (both from Life Technologies) was performed on co-cultures supernatants.
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bioRxiv - Immunology 2021Quote: ... populations were subject to either “rest” (low dose 30U/mL IL-2 only) or stimulation using Dynabeads Human T-Expander CD3/28 (ThermoFisher Scientific) at 1 million cells/mL at a ratio of 2 beads per cell for 48 hours ...
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bioRxiv - Cancer Biology 2022Quote: Peptide samples were analyzed on a Q Exactive HF-X Hybrid Quadrupole-Orbitrap Mass Spectrometer (Thermo Fisher Scientific, Rockford, IL, USA) coupled with a high-performance liquid chromatography system (EASY nLC 1200 ...
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bioRxiv - Immunology 2022Quote: ... and activated for 48h with 30 U/ml human IL-2 and anti-CD3-anti-CD28-beads (Dynabeads™ human T-Activator CD3/CD28, ThermoFisher). Retroviral packaging cells RD114 were seeded to reach a confluency of 60% on the day of transfection and subsequently transfected with plasmids containing the neoTCR-α- and -β-chain-sequences using TransIT®-293 (MirusBio) ...
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bioRxiv - Neuroscience 2022Quote: ... using a ViiATM7 Real Time PCR System or QuantStudio 6 FLEX Real Time PCR System (ThermoFisher Scientific Inc., Rockford, IL USA). Expression changes were calculated using the 2-ΔΔCt method normalized by Hprt as housekeeping gene ...
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bioRxiv - Physiology 2023Quote: ... Each cDNA sample was amplified with messenger RNA (mRNA) specific TaqMan Gene Expression Assays (IL-6, Mm00446190_m1; MCP-1, Mm00441242_m1; GAPDH, Mm99999915_g1; Thermo Fisher Scientific, Waltham, MA) on a CFX-96 real-time polymerase chain reaction (PCR ...
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bioRxiv - Neuroscience 2022Quote: ... Supernatants of cells maintained in normoxic condition or in 1% atmospheric oxygen in the presence of IL-1β (Thermo Fisher Scientific) for 24 hours were collected as described in (Kieran et al. ...
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bioRxiv - Neuroscience 2023Quote: ... NPCs to be terminally differentiated were passaged on three days after IL-6 stimulation (D21) into 50ng/ml poly-l-ornithine (Gibco; A3890401) and 20ng/ml laminin (Sigma ...
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bioRxiv - Genetics 2023Quote: ... they were briefly rinsed with PST and incubated in secondary antibodies and Hoechst 33342 (1:5,000; Thermo Fisher, Rockford, IL, USA) for DNA staining two hours at room temperature.
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bioRxiv - Microbiology 2024Quote: ... The cells were pelleted (400 x g for 10 minutes) and used further for analysis of cytokine mediators IFN-γ and IL-17 (ThermoFisher) for ELISA analysis ...
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bioRxiv - Cancer Biology 2024Quote: ... Glutathione determinations were normalized to the protein content of the insoluble pellet from the SSA extracts 2.5% SDS in 0.1 N bicarbonate using the BCA Protein Assay Kit (Thermo Scientific, Rockford, IL).
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bioRxiv - Genomics 2023Quote: Cell lysates were prepared in chilled RIPA buffer (25 mM Tris-HCL pH 7.6, 150 mM NaCl, 1% NP-40, 1% sodium deoxycholate, 0.1% SDS; Thermo Scientific, Rockford, IL) containing protease inhibitor (complete Mini ...
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bioRxiv - Neuroscience 2023Quote: ... Lateral striatum was dissected from each hemi-section and placed in HABG media (Hibernate A Media, BrainBits, Springfield, IL, HA500; 2% B27 Supplement, ThermoFisher, Cat #17504044; 0.26% Glutamax, ThermoFisher, Cat# 35050061). For each sample (ipsilateral and contralateral) ...
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bioRxiv - Molecular Biology 2022Quote: ... The protein contents of total cell lysates were determined using a BCA protein assay kit (Thermo Scientific Pierce, Rockford, IL, USA). The equal amounts of protein were separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) ...