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Citations for Thermo Fisher :
2801 - 2850 of 10000+ citations for IL 1beta Mouse CHO since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... 20 ng ml-1 GM-CSF (Biozym) or X38-Ag8 supernatant (1%) and from day 5 on additionally with 10 ng ml-1 IL-4 (Life technologies). At day 7 ...
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bioRxiv - Cell Biology 2023Quote: ... The secondary antibodies used were: Alexa Fluor Plus 488 (A32723, A32790, A32731, and A32814; Thermo Fisher Scientific Inc, Rockford, IL, USA), and Alexa Fluor 488 (A11055) ...
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bioRxiv - Biochemistry 2023Quote: ... The anti-F or G mAbs were prepared under serum-free conditions using HyClone SFM4MAb (Thermo Fisher Scientific Inc., Rockford, IL) and purified by protein G-Sepharose affinity chromatography.
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bioRxiv - Genetics 2023Quote: ... The whole assay was performed in a Nunc-Immuno MicroWell 96-well clear-flat-bottom plate (Thermo Fisher Scientific, Rockford, IL). The optical density at 570 nm was measured using Cytation 3 Multi-Mode Reader (Bio-Tek Instruments ...
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bioRxiv - Immunology 2023Quote: ... as well as between stratified patient plasma samples (n=7) over three time points using an IL-23 Human ELISA Kit (BMS2023-3, ThermoFisher, Canada), as per manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... The cells were then stained with anti-IL-1β at RT for 1hr and samples were acquired on an Attune NxT (Life Technologies). The data were analyzed with FlowJo software (Tree Star).
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bioRxiv - Cancer Biology 2023Quote: Peptides were resuspended in 200 mM HEPES (pH 8.5) and a 100µg aliquot of peptides was mixed with tandem mass tags (TMT, ThermoFisher Pierce, Rockford, IL) at a label to protein ratio of 2:1 ...
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bioRxiv - Neuroscience 2023Quote: ... made on the basis of DMEM/F12 with penicillin/streptomycin and supplements: 10% horse serum (Thermo Fisher Scientific, Rockford, IL, USA), 2 mM glutamine ...
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bioRxiv - Genomics 2023Quote: Cell lysates were prepared in chilled RIPA buffer (25 mM Tris-HCL pH 7.6, 150 mM NaCl, 1% NP-40, 1% sodium deoxycholate, 0.1% SDS; Thermo Scientific, Rockford, IL) containing protease inhibitor (complete Mini ...
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bioRxiv - Microbiology 2023Quote: ... were used as the secondary antibodies for detection and the color wasa developed using TMB substrate (Thermo Fisher Scientific, Rockford, IL).
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Pathogenic CD8 T cell responses are driven by neutrophil-mediated hypoxia in cutaneous leishmaniasisbioRxiv - Immunology 2023Quote: ... 20 U/mL recombinant human IL-2 at 37°C and 5% CO2 for 24 hours in RPMI 1640 (Gibco, Canada) containing 100 Units of penicillin and 0.1 mg/mL of Streptomycin (Sigma ...
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bioRxiv - Neuroscience 2024Quote: ... RT-qPCR reactions were carried out using Maxima SYBR Green/ROX qPCR MM (Thermo Scientific, product no. K0221, Rockford, IL, USA) in an Applied Biosystems StepOne Plus™ Real-Time PCR System (Foster City ...
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bioRxiv - Immunology 2024Quote: ... was used for sensitizing the cells in the presence of recombinant human IL-4 (Gibco, PeproTech, #200-04; 10 ng/mL) for three days ...
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bioRxiv - Genomics 2024Quote: ... and their concentration and final size distribution were evaluated using a Qubit HS Assay Kit (Thermo Scientific, Hanover Park, IL, USA) and pulse field gel electrophoresis (Pippin pulse ...
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bioRxiv - Genomics 2020Quote: Mouse ESCs (mESCs) were cultured in mouse ESC media that contains DMEM (Dulbecco’s modified Eagle’s medium) (Thermo Fisher Scientific) supplemented with 15% fetal calf serum (FCS ...
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bioRxiv - Immunology 2021Quote: ... mouse serum samples were added to rat anti-mouse IgE mAb-coated 96-well Nunc MaxiSorb plates (Thermo Scientific). An HRP-conjugated goat anti-mouse IgE antibody (SouthernBiotech ...
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Role of autophagy in sepsis-induced skeletal muscle dysfunction, whole-body metabolism, and survivalbioRxiv - Cell Biology 2021Quote: ... A total of 21,981 mouse genes were included in the Affymetrix Mouse Clariom S Assay (Affymetrix, Santa Carla, CA) and all steps were performed at the McGill University and Génome Québec Innovation Centre (Montréal ...
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bioRxiv - Immunology 2020Quote: ... HEK293T cells were transfected with N-terminally 3XFLAG and V5-tagged wild type mouse ZBP1 or Zα1α2-mutant mouse ZBP1 transducing vectors in the pLenti6 backbone (Life Technologies) (Maelfait et al. ...
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bioRxiv - Cancer Biology 2020Quote: ... Mouse anti-human CD20 APC-H7 and mouse anti-human CD73 PE were from e-Bioscience (Thermo Fisher Scientific). Monoclonal mouse anti-human Ki67 was from Dako Agilent Technologies (Les Ulis ...
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bioRxiv - Pathology 2021Quote: ... The supernatant was moved to a clean tube and incubated overnight with 0.5 μg of either goat anti-mouse KIM-1 or mouse anti-MUC1 (B27.29) antibodies and Protein G conjugated to Sepharose 4B (ThermoFisher). Beads were washed with 0.5 ml HEPES-buffered saline (HBS ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... LAMP1 was stained with a mouse primary antibody (DSHB 1D4B) and anti-mouse Alexa Fluor 488 (Thermo Fisher Scientific).
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bioRxiv - Immunology 2020Quote: TNF-α released by mouse peritoneal macrophages was measured by a Mouse TNF-α ELISA Kit (Invitrogen, BMS607-3TEN) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... 012-22384 Anti DYKDDDDK mouse IgG2b) using the Pierce Mouse IgG1 Fab and F(ab’)2 Preparation Kit (ThermoFisher). First ...
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bioRxiv - Genomics 2022Quote: Mouse ESCs (mESCs) were cultured in mouse ESC media that contains DMEM (Dulbecco’s modified Eagle’s medium) (Thermo Fisher Scientific) supplemented with 15% fetal calf serum (FCS ...
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bioRxiv - Pathology 2023Quote: ... and then with the appropriate secondary antibody (Alex Fluor 488 anti-mouse IgG2b, Alex Fluor 555 anti-mouse IgG2a, or Alex Fluor 647 anti-rabbit IgG; 1:1,000, Invitrogen). Slides were observed using an LSM 710 Zeiss confocal laser microscope ...
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bioRxiv - Genetics 2024Quote: ... followed by HRP-conjugated goat-anti mouse secondary antibodies at 1/20,000 dilution (Goat anti-mouse-HRP conjugate, cat# 31430, Thermofisher). The proteins were detected by SuperSignal chemiluminescent substrate (Thermo Fisher) ...
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bioRxiv - Cancer Biology 2021Quote: The concentration of H2O2 in APAM was measured using the Amplex Red Hydrogen Peroxide/Peroxidase Assay Kit (Thermo Scientific, Rockford, IL, USA) according to the manufacturer’s protocols as previously described (17) ...
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bioRxiv - Microbiology 2019Quote: Human IL-18 and TNFα were measured with the human IL-18 ELISA Kit (MBL, Woburn, Massachusetts) and human TNFα ELISA Kit (ThermoFisher, Waltham, Massachusetts) according to the manufacturers’ instructions using cell culture supernatant at a 1:5 dilution ...
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bioRxiv - Molecular Biology 2021Quote: ... and a human IL-6 siRNA pool (Horizon Discovery, LQ-007993-00-0005) were transfected using Lipofectamine™ RNAiMAX Transfection Reagent (Invitrogen, 13778) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... At days 15-21 after PH induction, SRT2104 (InvivoChem, Libertyville, IL) suspended in the warm corn oil (ACROS Organics, Fair Lawn, NJ) (100mg/kg/day ...
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bioRxiv - Neuroscience 2021Quote: ... The chemiluminescent Western blot detection kit (SuperSignal West Femto Chemiluminescent Substrate) and ATP determination kit were from Thermo Scientific (Rockford, IL, USA). The ELISA Kit for rat/mouse insulin ...
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bioRxiv - Microbiology 2021Quote: ... The protein concentration in the cell free extracts was determined with a BCA assay kit (Pierce, Thermo Scientific, Rockford, IL, United States). Enzyme assays were prepared as follows ...
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bioRxiv - Immunology 2020Quote: ... MIN6 cells were either exposed to pro-inflammatory cytokines (17U/mL IL-1β, 167U/mL TNFα and 17U/mL IFNγ; all cytokines were supplied by eBioscience Affymetrix, Paris, France) 254 nm UVB irradiation 10 mJ ...
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bioRxiv - Immunology 2019Quote: ... 11-7139-42 or 85BRD, cat.: 11-7136-42), IL-4-PE (8D4-8, cat.: 12-7049-42) (all from Thermo Fisher Scientific); TNFa-Pacific Blue (MAb11 ...
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bioRxiv - Neuroscience 2020Quote: ... until being separated isocratically by a reversed-phase C18 column with a flow rate of 0.6 mL/ min using a Dionex Ultimate 3000 HPLC system (pump ISO-3100SD, Thermo Scientific, Bannockburn, IL). Electrochemical detection was achieved using a CoulArray model 5600A coupled with a guard cell (model 5020 ...
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bioRxiv - Systems Biology 2020Quote: ... Cells were resuspended in 1.25 mL radioimmunoprecipitation assay buffer consisting of 12.5 μL of protease inhibitor (Halt Protease Cocktail Inhibitor, Thermo Fisher Scientific, Rockford, IL) to prevent enzymatic degradation upon cell lysis ...
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bioRxiv - Molecular Biology 2020Quote: EndoC-βH1 cells were treated with DIMT1 siRNA and lysates were isolated using a proprietary lysis buffer (Pierce, Thermo Scientific, (Rockford, IL). Co-IP was performed at 4°C unless otherwise indicated ...
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bioRxiv - Microbiology 2020Quote: ... The resulted peptides were cleaned up in a HyperSep filter plate with bed volume of 40 μL (Thermo Fisher Scientific, Rockford, IL). Briefly ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were transferred to medium containing CSS and transfected with 35-100 nM siRNA using the Lipofectamine RNAiMAX reagent (Invitrogen, Rockville IL) according to the manufacturer’s directions for a duration of 48-72 hours.
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bioRxiv - Bioengineering 2022Quote: ... CCL5 or IL-2 was performed according to the protocol of eBioscience™ Intracellular Fixation & Permeabilization Buffer Set (Cat.88-8824-00, Thermo Fisher). Briefly ...
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bioRxiv - Microbiology 2022Quote: Primary human CD4+ T cells were incubated with 20U/ml interleukin-2 (IL-2) alone or in combination with α-CD3/CD28 antibody-coated beads (100 μL/30 x 106 cells) (Gibco/Thermo Fisher) for 72 hours prior to infection with 100ng of p24 equivalent of pseudotyped pMorpheus-V5 ...
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bioRxiv - Microbiology 2022Quote: ... purified CD4+ T cells were maintained for two days in complete RPMI 1640 medium containing recombinant interleukin-2 (IL-2) (300 IU/ml) and phytohemagglutinin (1 μg/ml, Thermo Fisher Scientific), followed by 3 days in the presence of IL-2 alone ...
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bioRxiv - Immunology 2022Quote: ... 1% MEM NEAA and 50 Units/mL recombinant human IL-2 (Myltenyi; #130-097-745) with Dynabeads™ Human T-activator CD3/CD28 (Gibco, #11132D) the same day of the purification (day 0 ...
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bioRxiv - Neuroscience 2021Quote: ... at room temperature for 5 min and then the slides were mounted using Fluoromount(tm) (Life Technologies-Thermo scientific, Rockford, IL, USA) as a mounting medium.
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bioRxiv - Neuroscience 2020Quote: ... Protein concentrations in the tissue lysates were measured with the aid of a bicinchoninic acid protein assay kit (Thermo Scientific, Rockford, IL). TNF-α ...
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bioRxiv - Immunology 2021Quote: ... for Tcaim and Ubc or custom designed primer and probes for Il-2 and Ifn-γ (Eurofins Genomics) and TaqMan Universal PCR Master Mix (Thermo Fisher) on a 7500 Real Time PCR System (Applied Biosystems) ...
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bioRxiv - Immunology 2021Quote: ... IL-1-Ra plasma levels were determined of 20 patients positive and 12 patients negative for IL-1-Ra-Abs with a commercially available ELISA kit (Invitrogen/ThermoFisher #BMS2080) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... pH 7.4) with 1 mM phenylmethylsulphonyl fluoride (PMSF, USB Corporation, Cleveland, OH) and Pierce protease inhibitors with EDTA (ThermoFisher Scientific, Rockford, IL). Bacteria were lysed in a French pressure mini-cell by three cycles of 9,000 p.s.i ...
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bioRxiv - Immunology 2022Quote: ... fumigatus strains Af293 and CEA10 were grown at 37℃ for 5 days on Sabouraud’s Dextrose Agar (SBD; Difco, Fisher Scientific, Rockford, IL) or for 3 days on glucose minimal media slants (GMM) ...
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bioRxiv - Immunology 2022Quote: ... washed and added at 103 cells per well to various ratios of effector T cells (CTC or UTC population after overnight incubation with IL-15) in 96 well V-bottomed plates (NUNC, Thermo Fisher Scientific). After 4 hours of co-incubation ...