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Citations for Thermo Fisher :
2651 - 2700 of 6840 citations for H D Glu amc oh since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2024Quote: ... cDNA synthesis was performed with RevertAid H Minus First Strand cDNA Synthesis Kit (Thermo Scientific, K1632), with the following components ...
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bioRxiv - Cell Biology 2024Quote: ... 72 h later the cell nuclei were stained with 2 µM Hoechst (62249, Thermo Fisher Scientific) in PBS and the cells were counted using the Celigo image cytometer.
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bioRxiv - Cancer Biology 2024Quote: ... Cells were transfected after 48 h using Lipofectamine RNAimax (Life Technologies, USA, Cat No. 13778-150) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2024Quote: ... was washed by soaking it for 16 h in diethylpyrocarbonate (DEPC)-treated water (Thermo Fisher Scientific) on a Fisherbrand Multiplatform Shaker (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2024Quote: One microgram of RNA was reverse transcribed using Revert Aid H Minus Reverse Transcriptase (Thermo Scientific), and the RT product was used for expression analysis using Maxima SYBR Green/ROX qPCR Master Mix (Thermo Scientific ...
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bioRxiv - Cancer Biology 2024Quote: ... Gels were washed and stained for 1 h with Simply Blue™ Safe Stain (Life Technologies). After washing for 1 h ...
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bioRxiv - Cancer Biology 2024Quote: ... goat anti-rabbit (H+L) secondary HRP conjugate was purchased from Invitrogen (#31460, WB 1:5000), Actinomycin D was purchased from Sigma Aldrich (#A1410-2MG).
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bioRxiv - Developmental Biology 2024Quote: ... Tadpoles were blocked for 1 h at room temperature in 10% CAS-block (Invitrogen #00-8120) in PBT ...
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bioRxiv - Developmental Biology 2024Quote: ... and Goat anti-Mouse IgG (H+L) Secondary Antibody-HRP (Thermo Fisher Scientific, 31430, 1:2000). Protein bands were detected with ECL kit (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2020Quote: ... The resulting PCR products were subcloned into pENTR/d-TOPO vector (Thermo Fisher Scientific). After sequences were confirmed with an ABI3130 sequencer (ABI) ...
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bioRxiv - Plant Biology 2020Quote: ... The sequence was subsequently cloned in the pENTR/D-TOPO vector (Thermo-Fisher Scientific), to generate a pENTR-ANAC017 plasmid ...
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Vegetative nuclear positioning is required for calcium and ROS signaling in Arabidopsis pollen tubesbioRxiv - Plant Biology 2020Quote: ... NLS-YC3.6 and R-GECO1 were cloned into pENTR/D-TOPO vectors (Life Technologies) and then moved to LAT52pro::pH2GW7 via the Gateway® LR reaction (Life Technologies).
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bioRxiv - Plant Biology 2022Quote: ... The sequences were cloned into the Gateway vector pENTR/D-TOPO (Invitrogen, CA, USA) and transferred into recombination sites of the pANDA vector (Miki and Shimamoto ...
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bioRxiv - Cell Biology 2022Quote: Lysosomal proteolytic degradation was evaluated using DQ Red BSA (#D-12051; Thermo Fisher Scientific), a fluorogenic substrate for lysosomal proteases ...
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bioRxiv - Neuroscience 2021Quote: ... Glucose free media consisted of Neurobasal-A without D-glucose and sodium pyruvate (Invitrogen), 1X B27 without antioxidants (Invitrogen) ...
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bioRxiv - Bioengineering 2022Quote: ... where 50 μL of 0.2 mM Fluorescein-Di-β-D-Galactopyranoside (FDG; Life Technologies) is added as the fluorogenic substrate ...
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bioRxiv - Genomics 2020Quote: ... Samples passing quality control were analyzed using the human Clariom D Assay (ThermoFisher Scientific) and analyzed using Transcriptome Analysis Console (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: To make the nutrient supplemented plates for paralysis assays 10mM D(+)-glucose (Fisher Scientific), 0.3mM sodium homogamma linolenate solution (Nu-Chek Prep) ...
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bioRxiv - Bioengineering 2020Quote: ... The PCR product was cloned in the pENTR/D-TOPO vector (Thermo Fisher Scientific), sequenced (Fig ...
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bioRxiv - Cell Biology 2021Quote: ... HEK-293T were grown using DMEM + GlutaMAX + 4.5g/L D-glucose + Pyruvate (Gibco™) medium complemented with 10% FBS and penicillin-streptomycin ...
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bioRxiv - Biochemistry 2020Quote: ... The pellet was resuspended in M1 plating media (67ml D-MEM; 25mM HEPES (Invitrogen), 17.5ml M-199 (Invitrogen) ...
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bioRxiv - Neuroscience 2021Quote: ... and plated on Poly-D-Lysine/Laminin coated plates in Neurobasal medium (Thermo Fisher) containing neurotrophic factors (BDNF ...
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bioRxiv - Genetics 2021Quote: ... The amplified full-length cDNAs were cloned into pENTR/SD/D-TOPO vectors (Invitrogen), and then subcloned between the 35S promoter and YFP coding sequence in the destination plasmid pEarleyGate 101 ...
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bioRxiv - Microbiology 2020Quote: ... and induced with isopropyl b-D thiogalactoside (IPTG) in 1L luria broth (Thermofisher Scientific) containing ampicillin (Sigma-Aldrich) ...
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bioRxiv - Neuroscience 2020Quote: ... Then gBLOCK was cloned into an empty backbone vector pENTR/d-TOPO (ThermoFisher K240020) following manufacture instructions ...
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bioRxiv - Plant Biology 2021Quote: ... Amplified fragments were subcloned into the pENTR™/D-TOPO vector (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... the media was replaced by fresh medium containing 10ug/ml of Actinomycin D (Invitrogen) and was incubated for different time intervals at 37°C ...
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bioRxiv - Cancer Biology 2021Quote: ... adapted to final concentrations of 5 mM D-glucose (Thermo Fisher Scientific, Waltham, USA), 2 mM L-glutamine ...
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bioRxiv - Immunology 2022Quote: ... In house MACS separation buffer consisted of D-PBS with 2mM EDTA (ThermoFisher, 15575020) and 2% HI-FBS.
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bioRxiv - Plant Biology 2021Quote: ... Rx1-CC and Gpa2-CC were cloned initially into pENTR-D topo vector (Invitrogen). Sequence-verified fragments were then cloned into both pDEST-SCYNE(R)GW or pDEST-SCYCE(R)GW vectors by Gateway LR reaction as described (Gehl et al. ...
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bioRxiv - Developmental Biology 2021Quote: uL11 was amplified from w1118 embryonic cDNAs and subcloned into pENTR/D-TOPO (Invitrogen) (17) ...
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bioRxiv - Microbiology 2020Quote: ... The backbone of pENTR plasmid was amplified by PCR using primers pENTR_gibson_backbone_F and pENTR_gibson_backbone_R from the pENTR-D-TOPO cloning kit (Invitrogen). The resulting PCR product was subsequently treated with DpnI to remove methylated template DNA ...
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bioRxiv - Neuroscience 2020Quote: ... Bacteria were treated with 0.1 mM Isopropyl β-D-thiogalactoside (IPTG) (Thermo Fisher, AM9462) at 16°C for 16 hr to induce protein expression ...
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bioRxiv - Bioengineering 2021Quote: ... The transgene in pENTR/D-TOPO vector (pENTR™ Directional TOPO Cloning Kits, Invitrogen) was subcloned into vector pK-pGT43B-GW7 (Ratke et al. ...
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bioRxiv - Cancer Biology 2022Quote: ... Transcriptomic analysis was performed using Clariom™ D GeneChip array hybridization (Thermo Fisher Scientific). Single-strand cDNA preparation ...
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bioRxiv - Cancer Biology 2022Quote: ... the mice were anaesthetised by isoflurane inhalation and intraperitoneally injected with D-luciferine (Invitrogen). Luminescence signals were acquired using an IVIS SpectrumCT In Vivo Imaging System (PerkinElmer ...
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bioRxiv - Neuroscience 2022Quote: ... Smn and caz PCR products were subcloned into pENTR-D/TOPO vector (Life Technologies), fully sequenced ...
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bioRxiv - Microbiology 2020Quote: ... 0.5 μL of Oligo d(T)s (ThermoFisher Scientific, Cat No.: SO131, 100 μM), 1.0 μL of Ribolock RNase Inhibitor (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were seeded on poly-D-lysine coated glass cover slips in DMEM (Invitrogen) with 10% FBS and PenStrep (both Gibco) ...
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bioRxiv - Molecular Biology 2020Quote: ... at 11.5 dpc and a Mouse Clariom D array at 13.5 dpc (Thermo Fisher). 500 µg of total RNA were processed according to the manufacturer ...
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bioRxiv - Pathology 2020Quote: ... Ten independent microarrays were performed using the Clariom D arrays (Affymetrix-Genechip array, #902922) according the manufacturer’s protocol.
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bioRxiv - Synthetic Biology 2020Quote: ... 3.3 nM of 5-chloromethylfluoresecein di-beta-D-galactopyranoside (CMFDG) (Invitrogen, Waltham, MA, USA) was added to the reaction solution ...
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bioRxiv - Plant Biology 2020Quote: ... SRK2E and SRK2I cDNAs were previously cloned into pENTR/D-TOPO vector (Invitrogen, CA) (Umezawa et al. ...
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bioRxiv - Neuroscience 2020Quote: ... cells were seeded on poly-D-lysine and laminin coated co-verslips (Thermo Fisher) in a 24-well plate ...
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bioRxiv - Developmental Biology 2021Quote: The remaining 10% testis tissues were fixed by 1X D-PBS (Thermo Fisher Scientific) containing 4% paraformaldehyde/PFA (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2021Quote: ... Dead cells were excluded either via 7-aminoactinomycin D staining 1:1000 (7AAD, Invitrogen) or Sytox AAD (1:5000 ...
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bioRxiv - Genetics 2021Quote: ... coated with D-MEM/F-12 supplemented with 20% of KnockOut Serum replacement (Gibco) and 10 μg/mL of Basic Fibroblast Growth Factor (bFGF ...
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bioRxiv - Immunology 2020Quote: ... We used an in house MACS separation buffer comprised of D-PBS (Gibco, 59331C) with 2mM EDTA (ThermoFisher ...
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bioRxiv - Developmental Biology 2020Quote: ... primary antibody used was mouse anti-HuC/D (1:500; 16A11, Molecular Probes, USA), which was detected using Alexa Fluor 555 conjugated goat anti-mouse IgG diluted 1/1000 ...
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bioRxiv - Immunology 2020Quote: ... and D-glucose were used at 10mM in DMEM without any carbon (Gibco A1443001). For establishment of macrophage cell line with diminished mitochondrial mass ...