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Citations for Thermo Fisher :
2701 - 2750 of 10000+ citations for 7 Propyl 3 trifluoromethyl 1 2 benzisoxazol 6 ol since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... and ran on a QuantStudio 7 Flex (Thermo Fisher). The RNase P TaqMan Copy Number Reference Assay was used for normalization within the same sample ...
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bioRxiv - Molecular Biology 2023Quote: ... and ViiA-7 Real-Time PCR system (Applied Biosystems). Transcript RNA levels were normalized against β-actin reference gene following the 2-ΔCt method ...
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bioRxiv - Molecular Biology 2023Quote: ... and ViiA-7 Real-Time PCR system (Applied Biosystems).
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bioRxiv - Cancer Biology 2023Quote: ... and 7-AAD viability staining solution (Thermo Fisher Scientific), after treatment with a human FcR blocking reagent (Miltenyi Biotec) ...
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bioRxiv - Cell Biology 2023Quote: ... blocked with 7% goat serum (Gibco; Thermo Fisher Scientific), and incubated with primary antibodies followed by incubation with fluorescent secondary antibodies (Table 2) ...
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bioRxiv - Immunology 2023Quote: ... 5 μl of 7-AAD (Invitrogen, 00-6993-50) was included for dead cell exclusion.
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bioRxiv - Genetics 2023Quote: ... A clean glass slide (Fisher Scientific, 12-544-7) was dipped into freshly made PFA solution (1% paraformaldehyde adjusted to pH 9.2 using 1.25 M sodium borate ...
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bioRxiv - Cell Biology 2024Quote: ... Viability was controlled using 7-Aminoactinomycine D marker (Invitrogen). Fluorescence intensity of CTV and antibodies was measured with the SP6800 cytometer (Sony ...
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bioRxiv - Cell Biology 2024Quote: ... qPCRs were run on QuantStudio 7 (Applied Biosystems 4485701) and analyzed using the ΔΔCt method ...
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bioRxiv - Immunology 2024Quote: ... AT270 and HT-7 (Thermo Fisher Scientific, Waltham, MA) were used to capture/detect Tau and pTau181.
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bioRxiv - Bioengineering 2024Quote: ... Huh-7 cells were cultivated in DMEM (ThermoFisher Scientific) supplemented with 10% fetal calf serum (FCS ...
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bioRxiv - Molecular Biology 2024Quote: ... 7 μL of RNase I (Invitrogen, Cat. No. AM2249) was added to the clarified lysates and digestion was carried out for 1 hour at 4 °C ...
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bioRxiv - Microbiology 2022Quote: ... per well were distributed in Ibidi® µ-slide 8-well chambered coverslips and stained with 0.8 μM of the membrane specific fluorescent styryl dye N-(3-triethylammoniumpropyl)-4-(6-(4-(diethylamino) phenyl) hexatrienyl) pyridinium dibromide (FM4-64; Thermo Fisher Scientific, Waltham, MA, USA) in the presence of 0 μM (control) ...
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bioRxiv - Plant Biology 2019Quote: ... pollinated stigma was incubated in FM™ 4-64 Dye (N-3-Triethylammoniumpropyl-4-6-4-Diethylamino Phenyl Hexatrienyl Pyridinium Dibromide, Life Technologies T3166, 8.23 μM) for five minutes and subsequently washed in 1/2 Murashige and Skoog basal medium containing 10 % (w/v ...
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bioRxiv - Cell Biology 2020Quote: ... 2% BSA and 2 mM EDTA at 1 x 106 cells per 100 μl: anti-ITGA7 APC 1:500 (Invitrogen MA5-23555) and anti-SCA-1 FITC 1:50 (BD Pharmingen 557405) ...
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bioRxiv - Genetics 2020Quote: ... A single gRNA and single strand HDR oligo were co-transfected along with the gRNA (1-2 μg of each plasmid) into 1-2 × 106 WT ESCs using Lipofectamine 2000 (Invitrogen #1168-019) in a single well of a 6 well plate ...
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bioRxiv - Bioengineering 2024Quote: ... and HITI insert were first mixed with a ratio of 2:2:1 with the reagent P3000 (ratio of DNA: P3000 of 1:2) in Opti-MEM™ Reduced Serum Medium (Gibco™), and then added to Opti-MEM™ Reduced Serum Medium (Gibco™ ...
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bioRxiv - Genomics 2023Quote: ... coated dishes in N2 medium (high-glucose DMEM, 1% N-2, 2% B-27 and 1% penicillin/streptomycin, from Thermo Fisher Scientific) at a density of 2×105 cells/cm2 ...
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bioRxiv - Synthetic Biology 2021Quote: ... The final plasmids were assembled in a total volume of 20 µl using 100 or 200 ng of linearized ArEc-TDH3 or GR-306MP backbone with molar insert:plasmid ratio of 7:1 (116 or 281 ng of digested PCR product, respectively) and 1 µl of T4 DNA ligase (Thermo Fisher Scientific, catalog no. EL0011). The mixtures were incubated for 3 h at room temperature ...
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bioRxiv - Cell Biology 2021Quote: Cross-linking experiments of Sgo11-415 and CPCISB10-280 were performed using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC, Thermo Fisher Scientific) in the presence of N-hydroxysulfosuccinimide (NHS ...
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bioRxiv - Microbiology 2019Quote: ... and then induced to differentiate by culturing for further 3 days in a 3:1 mixture of DMEM and Ham’s F12 media (Invitrogen, Palo Alto, CA) containing FCS (10%) ...
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bioRxiv - Cell Biology 2022Quote: ... The C2C12 cells were transfected with 2 μg plasmids (L-Cas9n-EGFP:R-Cas9n-puro=3:1) by electroporation (1650 v/10 ms/3 pulses) with the Neon Transfection System Kit (Thermo Fisher Scientific). The cells were seeded overnight to 24-well plates containing an antibiotic-free complete mediums ...
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bioRxiv - Developmental Biology 2022Quote: ... Maturation of released oocytes was induced by incubating for 1h at 16C in 3 μM 1-Methyladenine (Fisher Scientific, 5142-22-3). All embryos were raised in 0.22 μm filtered sea water (FSW ...
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bioRxiv - Immunology 2022Quote: ... targeting SAMHD1 (sense RNA 5’-GCAGAUAAGUGAACGAGAUTT-3’, antisense RNA 5’-AUCUCGUUCACUUAUCUGCAG-3’) or the negative control #1 siRNA using RNAiMAX (ThermoFisher, 13778-075). 24 h after transfection ...
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bioRxiv - Microbiology 2024Quote: ... 1 μg of plasmid and 3 μL of Lipofectamine 2000 (m/v = 1:3) were diluted in 0.1 mL of Opti-MEM (Gibco, 31985070, Gaithersburg, MD). After 5 min of incubation ...
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bioRxiv - Microbiology 2024Quote: ... was added at a 1:2000 dilutions for 1 h at 37 °C, followed by adding TMB (3, 3, 5, 5′-tetramethylbenzidine) peroxidase substrate (Thermo Fisher Scientific) for 30 min ...
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bioRxiv - Immunology 2023Quote: ... and activated with a mixture of 400 mM 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride and 100 mM N-hydroxysuccinimide (Thermo Fisher Scientific). The activated chip was lawned with goat anti-human IgG Fc (50 µg/mL ...
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bioRxiv - Immunology 2023Quote: ... and activated with a mixture of 400 mM 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride and 100 mM N-hydroxysuccinimide (Thermo Fisher Scientific). The activated chip was coupled directly to mAbs diluted to 10 µg/mL in 10 mM sodium acetate (pH 4.5 ...
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bioRxiv - Cell Biology 2023Quote: ... 5’-GAGACCCUAUCCGUGAUUAtt-3’ and antisense: 5’- UAAUCACGGAUAGGGUCUCtt-3’ (Silencer Select, rat negative control #1; scrambled siRNA and all siRNAs were from Ambion, Life Technologies). Transfection complexes were prepared in accordance with the instructions provided by the manufacturer and added to 2×105 cells seeded per well in 24-well plates.
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bioRxiv - Microbiology 2023Quote: ... The chip was activated with a freshly prepared solution of 130 mM 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) (Pierce PG82079) and 33 mM N-hydroxysulfosuccinimide (Sulfo-NHS) (ThermoFisher Scientific 24510) in 0.1 M MES pH 5.5 using the SFC ...
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bioRxiv - Microbiology 2023Quote: ... The chip was activated with a freshly prepared solution of 130 mM 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride (EDC) (Pierce PG82079) and 33 mM N-hydroxysulfosuccinimide (Sulfo-NHS) (ThermoFisher Scientific 24510) in 0.1 M MES pH 5.5 using the SFC ...
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bioRxiv - Bioengineering 2021Quote: ... 50 μg of pre-aliquoted Tandem Mass Tag 6-plex (TMT-6, Thermo Scientific) was resuspended in 20 μL anhydrous acetonitrile ...
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bioRxiv - Cancer Biology 2022Quote: ... Plasma IL-6 was measured by immunoassay (ProQuantum Human IL-6 Immunoassay Kit, ThermoFisher) and run on the ABI 7300 (Applied Biosystems ...
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bioRxiv - Bioengineering 2023Quote: FsC[Fe3+] was labelled by 6-carboxyfluorescein (6-FAM) (Thermo Fisher Scientific, Waltham, USA) to indicate the targeting of FsC[Fe3+] to C ...
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bioRxiv - Immunology 2022Quote: ... The 40 mg/ml 4-HT stock solution was diluted 1:1 in Kolliphor EL (MilliporeSigma) 59.Before injection the solution was further diluted 1:3 in PBS (Gibco) and warmed to 37°C ...
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bioRxiv - Cell Biology 2020Quote: ... minced into 1-2 mm squares and digested with Collagenase Type I (2 mg/ml, Gibco) at 37°C for 1 hour with agitation ...
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bioRxiv - Biochemistry 2020Quote: ... Cells were split at 1:2 every 2 days using Trypsin-EDTA (ThermoFisher Scientific, Waltham, MA).
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bioRxiv - Cell Biology 2019Quote: ... minced into 1-2 mm squares and digested with Collagenase Type I (2 mg/ml, Gibco) at 37°C for 2 h with occasional agitation ...
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bioRxiv - Physiology 2023Quote: ... cardiomyocytes were incubated with 1 µM Fura-2- acetoxymethyl ester (Fura-2 AM, cell permeant, ThermoFisher) and 0.075% Pluronic F-127 (20% in DMSO ...
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bioRxiv - Developmental Biology 2021Quote: ... supplemented with 1% N-2 supplement (Invitrogen cat # 17502048), 1% Chemically Defined Lipid Concentrate (Invitrogen cat # 11905031) ...
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bioRxiv - Developmental Biology 2021Quote: ... and 0.2 mM 1-phenyl-2-thiourea (Acros Organics) for 24 h from 1 to 2 dpf.
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bioRxiv - Cancer Biology 2021Quote: ... 10 μM 2-ME and 1% penicillin/streptomycin (Gibco).
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bioRxiv - Neuroscience 2020Quote: ... or (2) 1:20 AlexaFluor 488 phalloidin (ThermoFisher #A12379) and 1:500 DAPI (Millipore Sigma #D9542 ...
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bioRxiv - Cell Biology 2021Quote: ... 10mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES, Gibco), 1% Penicillin/Streptomycin/L-Glutamate (P/S/G ...
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bioRxiv - Microbiology 2022Quote: ... blood was diluted 1:2 with RPMI-1640 (Gibco), layered on top of Ficoll-Paque Plus (GE Healthcare ...
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bioRxiv - Neuroscience 2022Quote: ... phospho-Synapsin 2 (Ser425) (1:5000, Invitrogen, PA5-64855), Synapsin (1:1000 ...
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bioRxiv - Biochemistry 2022Quote: ... 2 mM L-Glutamine and 1% Antibiotic-Antimycotic (Gibco). Cells were routinely tested for mycoplasma (Minerva Biolabs) ...
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bioRxiv - Cancer Biology 2022Quote: ... Matrix Metalloprotease 2 (MMP2, Thermo Fisher Scientific, 1:1000), Matrix Metalloprotease 9 (MMP9 ...
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bioRxiv - Immunology 2020Quote: ... 2 mM L-glutamine and 1 mM HEPES (Gibco).
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bioRxiv - Developmental Biology 2021Quote: ... 1% CTS N-2 supplement (Gibco; Thermo Fisher Scientific), 0.5% GlutaMAX (Thermo Fisher Scientific) ...