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Citations for Thermo Fisher :
2551 - 2600 of 10000+ citations for 7 Propyl 3 trifluoromethyl 1 2 benzisoxazol 6 ol since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... was aspirated and the fixed cells treated with 2-3 drops of ProLong Gold Antifade Mountant (Invitrogen #P36930) before imaging ...
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bioRxiv - Plant Biology 2020Quote: ... seedlings were washed for 2-3 min in water and transferred to a chambered cover glass (Thermo Scientific), and imaged either as described above or using a Leica DM5500 wide field microscope (GFP filtercube ex ...
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bioRxiv - Molecular Biology 2020Quote: ... RNA was reverse-transcribed by addition of 3 μl of RT mix (2 μl 5x SSIV buffer [Invitrogen] ...
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bioRxiv - Microbiology 2020Quote: ... Myoblat and myotubes viability was determined by 3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide (MTT; Life Technologies) metabolization.
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bioRxiv - Cancer Biology 2020Quote: ... then assayed in a standard MTT assay: 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (ThermoFisher, Waltham, MA) [39] ...
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bioRxiv - Biochemistry 2022Quote: ... equipped with an Acclaim Pepmap C18 trap column (2 cm * 75 µm, particle size: 3 µm; Thermo Fisher) and a C18 analytical column (50 cm * 75 µm ...
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bioRxiv - Genetics 2021Quote: ... Gels were run at 120V for 2-3 hours in Bolt MES SDS Running Buffer (Thermo Fisher Scientific) prior to protein transfer to Amersham Protran nitrocellulose blotting membrane (GE Healthcare ...
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bioRxiv - Neuroscience 2021Quote: ... supplemented with NeuroCult™ SM1 neuronal supplement (STEMCELL), L-glutamine (2 mM, PAA) and 3% horse serum (Invitrogen) at 37°C in 5% CO2 ...
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bioRxiv - Cancer Biology 2022Quote: Tail and ear fragments from C57/B6 or mT/mG mice were dissected and incubated in 2-3 hours at 37 °C in 950 µL of Dulbecco’s modified Eagle’s medium (DMEM, Invitrogen), supplemented with 10% inactivated fetal bovine serum (FBSi ...
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bioRxiv - Bioengineering 2019Quote: ... Lysis was done in 2% SDS for 3 min at 95 °C in a thermomixer (Thermo Fisher Scientific), followed by digestion of DNA with Benzonase at 37 °C for 1.5 h ...
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bioRxiv - Cell Biology 2020Quote: ... and induced to differentiate in myotubes for 2-3 days in differentiation media (DM: IMDM (Gibco, 21980-032) + 2% of horse serum (Gibco ...
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bioRxiv - Cell Biology 2021Quote: ... cells were treated with DMNB-caged cAMP (4,5-Dimethoxy-2-Nitrobenzyl Adenosine 3’,5’-Cyclicmonophosphate, Molecular Probes, D1037) for at least 30 minutes prior to imaging at a final concentration of 1 mM ...
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bioRxiv - Immunology 2020Quote: Cell viability was estimated by a quantitative colorimetric assay described for human granulocytes which were based on metabolic reduction of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT, Invitrogen) into coloured product formazan (Oez ...
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bioRxiv - Developmental Biology 2022Quote: ... They were then immobilized on glass slides using 2% agarose and injected with 1,1’-dioctadecyl-3,3,3′,3′-tetramethylindocarbocyanine perchlorate (DiI, Molecular Probes) or 3,3′-dioctadecyloxacarbocyanine perchlorate (DiO ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were washed twice with PBS and loaded with 3 µM Fura-2 AM (Thermo Fisher, Waltham, MA) diluted in a modified Krebs-Ringer buffer solution [KRBH ...
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bioRxiv - Plant Biology 2022Quote: ... equipped with an Acclaim Pepmap C18 trap column (2 cm · 75 µm, particle size: 3 µm; Thermo Fisher) and a C18 analytical column (50 cm · 75 µm ...
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bioRxiv - Immunology 2022Quote: ... Primary B cells were plated at 2-3×106 cells/ml in primary B cell medium (DMEM (Gibco) containing 10% FBS (Sigma) ...
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bioRxiv - Genomics 2024Quote: ... Fresh medium was added every 2-3 days and cells were passaged using 1X TrypLE Express (Life Technologies).
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bioRxiv - Microbiology 2024Quote: ... The filtrate was acidified to a pH of 2-3 with OmniTrace HCl (ThermoFisher Scientific, Waltham, MA, USA) and extracted via solid phase extraction with styrene-divinylbenzene polymer columns (Bond Elut PPL ...
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bioRxiv - Cell Biology 2024Quote: ... and a lentiviral transfer plasmid (2:3:4 ratio by mass) using Lipofectamine 3000 (Thermo Fisher Scientific L3000015). Viral supernatant was harvested 48h after transfection and filtered through 0.45 µm cellulose acetate filters (Corning 431220) ...
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bioRxiv - Plant Biology 2023Quote: ... 3 µg RNA from each sample was used to mix with 2×RNA loading dye (Thermo Fisher Scientific), denatured at 65℃ for 10 min ...
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bioRxiv - Cancer Biology 2024Quote: ... or using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) reagent (Fisher Scientific, AC158992500, Waltham, MA, USA) at 570 nm ...
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bioRxiv - Pathology 2023Quote: Tail and ear fragments from mT/mG mice were dissected and incubated in 2-3 hours at 37 °C in 950 µL of Dulbecco’s modified Eagle’s medium (DMEM, Invitrogen), supplemented with 10% inactivated fetal bovine serum (FBSi ...
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bioRxiv - Genetics 2023Quote: ... 3-5 million cells or approximately 15 ug of DNA crosslinked with 2% PFA (Fisher Scientific F79-500) were used as input per reaction ...
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bioRxiv - Cell Biology 2023Quote: ... cells were treated with DMNB-caged cAMP (4,5-Dimethoxy-2-Nitrobenzyl Adenosine 3’,5’-Cyclicmonophosphate, Molecular Probes, D1037) for at least 30 min prior to imaging at a final concentration of 1 mM ...
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bioRxiv - Cancer Biology 2023Quote: Cell proliferation was assayed by reduction of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT; Invitrogen, M6494). MTT was freshly dissolved into PBS at a stock concentration of 12 mM and diluted into phenol red-free DMEM with 10% FBS for a final MTT concentration of 2 mM ...
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bioRxiv - Biochemistry 2022Quote: ... 2-3 µL of 5 µM protein solution was introduced directly into Q-Exactive UHMR mass spectrometer (ThermoFisher) through gold coated capillary needles that were prepared in-house30 ...
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bioRxiv - Microbiology 2023Quote: ... Proteins were migrated for 2-3 h at 120 V in NuPAGE MOPS SDS running buffer (#NP0001, Invitrogen) and transferred onto a PVDF membrane (#1704156 ...
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bioRxiv - Biochemistry 2024Quote: ... 2 μl of cDNA were mixed with 3 μl of PowerUp™ SYBR™ Green Master Mix (ThermoFisher) containing 1 μM of forward and reverse primer ...
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Validating Minimally Invasive Laser Doppler Flowmetry for Serial Bone Perfusion Measurements in MicebioRxiv - Bioengineering 2019Quote: Systemic inflammation was examined by quantifying serum concentrations of the proinflammatory marker interleukin 6 (IL-6) with an ELISA (IL-6 Mouse ELISA kit, KMC0061, Invitrogen, Carlsbad, CA). Samples were prepared according to the manufacturer’s instructions and measured using a plate reader (Synergy H1 ...
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bioRxiv - Microbiology 2021Quote: ... HEK293T cells were transfected with (pRMB EDEN15) and BASU plasmids (6:1 ratio) using Lipofectamine 2000 (1:1 ratio, Life Technologies, California, USA). 40 hours post-transfection ...
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bioRxiv - Cancer Biology 2021Quote: ... 6 hours prior to collection organoids were incubated with 1 μM EdU (Thermo fisher, #C10636).
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bioRxiv - Immunology 2019Quote: ... pneumoniae for 6 hours in 1% FCS phenol free alpha MEM (base media, Life Technologies). Cells were washed three times in PBS and gently lifted from the plate using a cell scraper in 300μl of base media supplemented with 1mM EDTA ...
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bioRxiv - Neuroscience 2022Quote: ... using samples diluted to 1:1000 while interleukin (IL)-6 (Thermo Fisher Scientific, Cat. #BMS625) was evaluated using samples diluted 1:2 ...
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bioRxiv - Immunology 2022Quote: ... in 6-well plates and cultured in 1 ml/well RPMI-1640 medium (Thermo Fisher) containing 4% B-27 (Life Technologies) ...
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bioRxiv - Biochemistry 2019Quote: ... Sf9 cells were grown in 1X 10 6 for 1 litre of Sf900 II (Gibco) media in presence of antibiotic and antimycotic (Gibco ...
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bioRxiv - Cell Biology 2022Quote: ... Suspension cells (THP-1) were first seeded for 6 h on poly-D-lysine (Gibco)-coated wells before starting the assay ...
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bioRxiv - Systems Biology 2022Quote: ... with the addition of 6 μl ERCC Spike-In Control Mix 1 (Ambion Thermo Fisher) diluted 1:10,000 ...
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bioRxiv - Systems Biology 2022Quote: ... with the addition of 6 μl ERCC Spike-In Control Mix 1 (Ambion Thermo Fisher) diluted 1:10,000 ...
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bioRxiv - Neuroscience 2022Quote: ... Fresh media was added and passaged 1:6 using the StemPro EZpassage tool (Life Technologies) as per the manufacturer’s instructions.
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bioRxiv - Biochemistry 2023Quote: ... The GLT-1 encoding sequence was subcloned into pcDNA™6 V5 Myc (Thermo Scientific).
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bioRxiv - Systems Biology 2023Quote: Approximately 1*10^6 UWB1.289 were lysed in 650 µl Tri reagent (Thermo Fisher, #AM9738) and bead milled with the following settings ...
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bioRxiv - Molecular Biology 2024Quote: ... arteries were incubated for 6 minutes in collagenase type IV (1 mg/mL, Life Technologies) and porcine pancreatic elastase (1 U/mL ...
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bioRxiv - Microbiology 2023Quote: ... and undigested chromatin were adjusted to 810μl with MCN-digestion buffer with 0.005M EGTA and cross-linked by adding 135 μl of buffered 7 % formaldehyde solution (7 % CH2O, 140 mM Na2HPO4 pH 8.67) using 16 % CH2O methanol-free ampules (Thermo Scientific Cat #28906), to a final concentration of 1 % ...
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bioRxiv - Developmental Biology 2024Quote: ... Embryos were blocked in 2% goat serum + 2% Bovine Serum Albumin for 2 hours at room temperature before primary antibody (GFP, 1:500, Invitrogen #A6455) was added and the embryos were incubated overnight at 4°C with rocking ...
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bioRxiv - Cell Biology 2020Quote: ... rabbit anti-centrin-3 (1:1000; PA5-35865; Thermo Scientific, Rockford, USA), rabbit anti-acetylated α-tubulin (1:800 ...
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bioRxiv - Cell Biology 2020Quote: ... 1% [w/w] P/S [Gibco] and 1 % [w/w] Glutamax [Gibco]) supplemented with 1 μM TO-PRO®-3 (Thermo Fisher Scientific). After 16 h ...
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bioRxiv - Immunology 2021Quote: ... 3 μl of a 1:400,000 dilution of ERCC synthetic mRNAs (ThermoFisher) were added and total RNA was isolated using a Quick-RNA MicroPrep Kit (Zymo Research ...
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bioRxiv - Neuroscience 2022Quote: ... and nuclear dye (TO-PRO-3 Iodide, 1:400, Thermo Fisher T3605) were added to the blocking solution ...
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bioRxiv - Neuroscience 2022Quote: ... and a 1:400 dilution of TO-PRO-3 (Thermo Fisher T3605) for 24 hr at 4°C in the dark ...