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Citations for Thermo Fisher :
2501 - 2550 of 10000+ citations for 7 Propyl 3 trifluoromethyl 1 2 benzisoxazol 6 ol since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2019Quote: Control and VDR-KD cells were plated at 1.0 × 1010 cells/well in 2 ml of growth medium in 6-well plates (Nunc, Roskilde, Denmark). Both myoblasts and myotubes were maintained and harvested as described previously ...
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bioRxiv - Biochemistry 2020Quote: ... Nuclei were stained with 10.9 μΜ 4’,6-diamidino-2-phenylindole (DAPI) and cover glasses were mounted in ProlongGold (Life Technologies). Cells were incubated 3x for 5 min in blocking solution to remove unbound antibodies ...
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bioRxiv - Immunology 2021Quote: The Fab fragments of Clone 2 and Clone 6 were generated from full length IgGs of Clone 2 and Clone 6 using a commercial PierceTM Fab Preparation Kit (Thermo Fisher). All procedures were performed following the manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... Reporter plasmids (2 μg) were transfected into HEK-293 cells in 6-well plates at ∼60-90% confluency using Lipofectamine 3000 (ThermoFisher Scientific). Cells were harvested after 1 day by aspirating the media and resuspending the cells in 1 mL Trizol reagent (ThermoFisher Scientific) ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were treated with RSL3 for 2 h or IKE for 6 h together with the indicated compounds before BODIPY 581/591 C11 (Thermo Fisher) was added in a final concentration of 2 μM per well and incubated for 30 min at 37°C ...
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bioRxiv - Neuroscience 2022Quote: ... Slices were then dipped in the secondary antibody-containing buffer with the nuclear dye 4′,6-diamidino-2-phenylindole (DAPI, Cat# D1306, Life Technologies-Invitrogen ...
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bioRxiv - Molecular Biology 2023Quote: Phosphor imaging of [γ-32P] radio-labelled DNA sequences was accomplished through 6% denaturing PAGE constituted of 1X TBE (89 mM Tris base, 2 mM EDTA and 89 mM boric acid, Fisher Scientific) (57) ...
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bioRxiv - Neuroscience 2022Quote: ... Nuclear staining of glass-mounted brain sections was performed by 10 min incubation with PBS containing 2.5% DAPI (4’,6-diamidino-2-phenylindole, Thermo Fisher Scientific). Sections were embedded in mounting medium containing 1,4-diazabicyclooctane (DABCO ...
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Pyruvate kinase M2 regulates Japanese encephalitis virus replication by interacting with NS1 proteinbioRxiv - Microbiology 2024Quote: ... Neuro-2a cells were cultured on 6-well plates and then transfected with a total of 2 µg of an expression plasmid using Lipofectamine 2000 (Invitrogen, USA) as per the instructions provided by the manufacturer.
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bioRxiv - Cancer Biology 2024Quote: ... the tissue specimens were stained with 4′,6-diamidino-2-phenylindole (DAPI) for 10 min and mounted in VECTASHIELD Vibrance Antifade Mounting Medium (ThermoFisher Scientific). Akoya Biosciences’ PhenoImager HT (formerly known as Vectra Polaris Automated Quantitative Pathology Imaging System ...
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bioRxiv - Cell Biology 2024Quote: ... and these values were re-calibrated based on measurements of NIST-certified polystyrene beads (2 µm and 6 µm in diameter, Duke Standards, Thermo Scientific).
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bioRxiv - Immunology 2024Quote: ... non-sun-exposed skin as previously described(13) and used at passages 2-6 and grown in Epilife medium (Gibco, #MEPI500CA) with added human keratinocyte growth supplement (10ul/ml medium) ...
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bioRxiv - Immunology 2023Quote: ... Nuclei were detected and cover slips were attached using 4′,6-diamidino-2-phenylindole dihydrochloride (DAPI) containing Prolong Diamond Anti-Fade (Life Technologies). Slides were imaged on a Keyence BX-X810 microscope.
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bioRxiv - Cell Biology 2023Quote: ... A final wash with PBS was performed before mounting the cells in ProLong® Gold Antifade Mounting medium with DAPI (4’,6-diamidine-2-phenylindole)(Invitrogen).
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bioRxiv - Molecular Biology 2023Quote: ... After 3 washes cells were stained with a combination of Alexa Fluor secondary antibodies and 4′,6-Diamidino-2-Phenylindole (DAPI; Molecular Probes) for 1 h at room temperature in the dark ...
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bioRxiv - Cell Biology 2023Quote: ... and ProLong™ Gold antifade reagent with 4′,6-diamidino-2-phenylindole (DAPI) (P36941) or without (P36930) was purchased from Invitrogen.
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bioRxiv - Molecular Biology 2023Quote: ... SVG-A Notch2-HaloTag cells were transfected in 6 well plate format with 2 µg of DNA per well using Lipofectamine 2000 (ThermoFisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: ... Protein pellets were resuspended in urea buffer (6 M urea, 2 M thiourea, 100 mM ammonium bicarbonate, pH 8.0) and quantified using EZQ (Invitrogen/Life Technologies).
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bioRxiv - Cancer Biology 2023Quote: ... a dry transfer was completed at 30V for 6 min with PVDF stacks in an iBlot 2 transfer instrument (Thermo Fisher). After gel transfer ...
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bioRxiv - Bioengineering 2023Quote: HEK293 cells were seeded on a 6-well plate (2×105 cells per well) and were cultured in low glucose Dulbecco’s Modified Eagle Medium (Thermo Fisher Scientific) supplemented with 10% FBS and 1% sodium pyruvate ...
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bioRxiv - Bioengineering 2023Quote: ... the hydrogel is first activated with sulfosuccinimidyl 6-(4′-azido-2′-nitrophenylamino) hexanoate (Sulfo-SANPAH, Pierce, Thermo Fisher Scientific, Waltham, MA). Here the remaining washing buffer around the gel edge is removed to ensure the coating solution can be held on top of the gel ...
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bioRxiv - Developmental Biology 2023Quote: ... Antigen unmasking was performed by microwaving the slides for 2 × 10 min in citrate buffer solution (pH 6) (Thermo Fisher Scientific). Sections were then blocked for 1 hour at room temperature with 10% normal donkey serum (Chemicon International Inc. ...
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bioRxiv - Cell Biology 2023Quote: ... and coverslips were mounted using Prolong Gold Antifade Reagent with 4′,6-diamidino-2-phenylindole (DAPI) (Life Technologies, Carlsbad, CA, USA). Cells were viewed using an Olympus IX81 or Olympus BX53 epifluorescence microscope ...
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bioRxiv - Biochemistry 2023Quote: ... Sf9 cells were seeded at 5·105 cells/ml in a 6-well plate in a total volume of 2 ml of Sf-900-II SFM media (10902088, ThermoFisher Scientific). Bacmids (see above ...
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bioRxiv - Bioengineering 2023Quote: ... sections were counterstained with 1 mg/mL of 4’,6-diamidino-2-phenylindole (DAPI) and 0.1 nmol units/ml of Phalloidin ATTO647N and mounted with Fluoromount-G (Thermo Fisher Scientific). The slides were acquired using a confocal (Leica SP8 ...
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bioRxiv - Neuroscience 2023Quote: ... including Camuiα were transfected into HEK293S GnTI-cells (2 x 106 cells per well in 6-well plates) cultured in FreeStyle 293 (Thermo Fisher), using the TransIT2020 transfection reagent (Mirus Bio) ...
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bioRxiv - Developmental Biology 2024Quote: ... and counterstained with 5 μg/mL of 4′,6-diamidino-2-phenylin-dole (DAPI) or propidium iodide (Molecular Probes, Life Technologies) for 10 min ...
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bioRxiv - Developmental Biology 2024Quote: ... and counterstained with 5 μg/mL of 4′,6-diamidino-2-phenylin-dole (DAPI) or propidium iodide (Molecular Probes, Life Technologies) for 10 min ...
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bioRxiv - Neuroscience 2024Quote: ... Concentration of each sample was adjusted to 2 μg/μl and then mixed with 6 X SDS-containing Laemmli buffer (ThermoFisher Scientific). Subsequently ...
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bioRxiv - Bioengineering 2023Quote: ... Hydrogels were washed extensively with PBS and activated via photoirradiation (365 nm, 0.8 mW for 5 minutes) with sulfosuccinimidyl-6-4’-azido-2’-nitrophenylamino hexanoate (Sulfo-SANPAH; Thermo Scientific Pierce). To do so ...
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bioRxiv - Cancer Biology 2023Quote: ... TRAIL blocking ab was purchased from Santa Cruz TRAIL Antibody (RIK-2): sc-56246, and IFN gamma blocking Monoclonal Antibody (NIB42, #6-7318-81) was purchased from Thermo Fisher. The blocking mAb was added when NK cells were combined with tumor cells.
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bioRxiv - Immunology 2023Quote: ... Invitrogen E-Gel EX Agarose Gels 4%, and DAPI (4’,6-Diamidino-2-Phenylindole, Dihydrochloride, D1306) were purchased from Thermo Fisher Scientific ...
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bioRxiv - Genomics 2024Quote: ... iPSCs were plated at high density (2 million cells/well in a 6 well plate) on hESC-qualified Geltrex (Gibco #A1413302), then cultured in neural induction media at high density with ROCK inhibitor Y27632 (StemCell Tech ...
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bioRxiv - Immunology 2024Quote: ... For T cell panels (Supplemental table 2, 4, 5, 6) we fixed cells with the FOXP3 Fixation/Permeabilization Buffer Kit (Thermo Fisher) and conducted intracellular/nuclear stains using the FOXP3 Permeabilization Buffer (Thermo Fisher ...
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bioRxiv - Cancer Biology 2024Quote: ... RIPA Lysis and Extraction Buffer (CAT NO. 89901), and DAPI (4’,6-Diamidino-2-Phenylindole, Dilactate) (CAT NO. D3571) were purchased from Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2024Quote: ... 3D iHeps (Day 22) were incubated for 2 hours with HCM media containing 1μmol/L of 6-CDF (Thermofisher, #C-369). Samples were then washed with PBS for 15 minutes before staining with Hoescht in HCM.
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bioRxiv - Molecular Biology 2024Quote: ... coverslips were mounted on glass slides using ProLong Gold antifade (4′,6-diamidino-2-phenylindole) DAPI to counterstain the nuclei (Thermofisher, #P36935).
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bioRxiv - Microbiology 2024Quote: ... 2X105 cells were seeded into a 6-well plate and transfected using a total of 2 µg of plasmid using Lipofectamine 3000 (Invitrogen, USA) as per the manufacturer’s instruction ...
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bioRxiv - Immunology 2021Quote: ... biotinylated with 7:1 biotin-to-antibody using EZ-Link Sulfo-NHS-LC-Biotinylation Kit (21435, Thermo Fisher Scientific), was prepared in dilution buffer ...
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bioRxiv - Neuroscience 2019Quote: ... Plasmids pCAG-dsRed and Pak3K297R transfection was performed at 7 d in vitro (DIV) with 1 µg of DNA and 1.5 µl of Lipofectamine (Invitrogen), as described in manufacturer’s manual (Invitrogen) ...
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bioRxiv - Cell Biology 2022Quote: Cell viability after 1 and 7 days was examined using Calcein AM (Thermo Scientific, Breda, the Netherlands, CCID: 390986) for live cells and propidium iodide (PI ...
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bioRxiv - Microbiology 2022Quote: ... using qScript 1-Step SYBR Green qPCR kit (Quantabio) on a ViiA 7 Real-Time PCR System (Applied Biosystems); 16S rRNA was used for normalization ...
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bioRxiv - Molecular Biology 2023Quote: ... Samples were pelleted at 3,000 g, aspirated, resuspended in SYTOX buffer (500 µL 50mM sodium citrate [pH 7], 1 µM SYTOX green (Invitrogen)) ...
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bioRxiv - Genomics 2022Quote: ... mouse pancreatic islets isolated from RipCre-tdTomato mice were trypsinized for 7 minutes with 1 mM of trypsine (Gibco). Dissociated pancreatic cells were directly run into an Influx sorter (Becton Dickinson® ...
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bioRxiv - Cancer Biology 2022Quote: ... 7*103 single cells derived from second-generation spheres were labeled with 1 mM CMFDA (Life Technologies, cat# C7025) for 30 minutes and added on top of the mesothelial monolayer ...
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bioRxiv - Cell Biology 2024Quote: ... The pellet was resuspended in PBS containing 1:1000 (v/v) 7-AAD viability dye (Invitrogen, catalog number A1310). 500 cells per well were sorted on a BD FACSAria III sorter using a 100-micron nozzle in 4-way purity precision mode into an Eppendorf twin.tec 384 LoBind plate containing 1µl of 0.2% DDM ...
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bioRxiv - Molecular Biology 2024Quote: ... supplemented with 7% Fetal Bovine Serum (FBS) (MilliporeSigma, catalog F4135) and 1% Penicillin/Streptomycin (P/S) (Gibco, catalog 15140163) at 37°C with 5% CO2 ...
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bioRxiv - Bioengineering 2019Quote: DSS-treated mice received 3 cycles of 2% (weight/volume) DSS (40,000 MW, Alfa Aesar, Thermo Fisher, France) in drinking water for 5 days ...
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bioRxiv - Cell Biology 2019Quote: ... and induced to differentiate in myotubes for 2-3 days in differentiation media (DM: IMDM (Gibco, 21980-032) + 2% of horse serum (Gibco ...
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bioRxiv - Neuroscience 2021Quote: ... Cultured neurons were incubated with 3 μg/mL of the ratiometric calcium indicator Fura-2 AM (Life Technologies) in external recording solution for 30 min at 37°C and 5% CO2 ...