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Citations for Thermo Fisher :
2651 - 2700 of 4608 citations for 6 HYDROXY CHRYSENE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... Finally samples were counter stained for nucleus with 1 μM DAPI (4′, 6-diamidino-2′-phenylindoldihydrochloride; Thermo Fisher Scientific). Zeiss inverted Axio Observer fluorescent microscope (Zeiss ...
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bioRxiv - Cell Biology 2019Quote: ... dsRNA for knocking-down Par-6 or aPKC was synthesized with MEGAscript T7 Transcription Kit (Ambion, Thermo Fisher Scientific) according to the manufacurer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: ... dsRNA for knocking-down Par-6 or aPKC was synthesized with MEGAscript T7 Transcription Kit (Ambion, Thermo Fisher Scientific) according to the manufacurer’s instructions ...
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bioRxiv - Developmental Biology 2019Quote: ... 100×103 cells / well cultured in 6 well plates were transfected with 0.8 μg each plasmid DNA using Lipofectamin2000 following the manufacturer’s protocol (ThermoFisher Scientific) and analysed 48 hours later.
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... extended with a nucleic acid sequence encoding for 6 histidine residues (His-tag) and cloned into the mammalian expression vector pcDNA3.1(+) (Invitrogen). The soluble antigen was produced by transient gene expression in CHO cells as described previously [38] and purified from the cell culture medium by Ni-NTA resin (Roche) ...
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bioRxiv - Biophysics 2020Quote: ... insect cells (cell density at 5 x 105 cells/mL) using 6 μL of CellFectin reagent and 100μL of SFM II media with antibiotics (penstrep, 0.5X) (Gibco™) and allowed to grow for 1 hour at 28°C ...
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bioRxiv - Neuroscience 2020Quote: ... L4-6 ganglia were isolated from adult male Sprague Dawley rats (175-250 g) and dissociated with collagenase (Invitrogen). Cells were washed and then plated with DMEM/F12 (Cellgro ...
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bioRxiv - Cancer Biology 2020Quote: ... Proton transport was measured using ATP-dependent quenching of 9-amino-6-chloro-2-methoxy-acridine (Acridine Orange, ThermoFisher) fluorescence quenching for isolated vacuoles as previously described21
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bioRxiv - Immunology 2021Quote: ... Nuclei were stained with 4′,6-diamidino-2-phenylindole and were embedded with ProLong Gold mounting medium (Life Technologies). ImageJ/Fiji software (National Institutes of Health ...
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bioRxiv - Immunology 2021Quote: ... kidneys were wax-embedded and sliced into 6 μm sections onto SuperFrost Plus™ adhesion slides (Thermo Fisher Scientific). Slides were air-dried O/N at RT and ...
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bioRxiv - Neuroscience 2020Quote: Human sections were counterstained with 4’,6-diamidino-2-phenylindole (DAPI) and coverslips were mounted using Prolong Gold (Invitrogen) for imaging ...
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bioRxiv - Cell Biology 2019Quote: ... hippocampi were dissected from E18 Sprague-Dawley rat embryos or E17 C57BL/6 mice embryos and treated with 0.25% trypsin in HBSS (Invitrogen) for 25 min at 37°C ...
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bioRxiv - Microbiology 2021Quote: Cells were reverse-transfected (transfected while plating) in 6-well plates (3.5 -5 × 105 cells per well) using Lipofectamine RNAimax transfection reagent (Invitrogen), following standard protocols ...
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bioRxiv - Cell Biology 2021Quote: ... the cells were mounted in ProLong Gold antifade reagent supplemented with 4′,6-diamidino-2-phenylindole (DAPI; Molecular Probes). Conventional immunocytochemical staining was done to quantify the activated PAK1 and actin in oAβ and IPA-3 treated cells using phospho-PAK1 (Thr423)/PAK2 (Thr402 ...
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bioRxiv - Biochemistry 2020Quote: ... according to the manufacturer’s instructions and diluted 1:6 with DNAse/RNAse-free water (Thermo Fisher, Waltham, MA, USA). qRT-PCR was performed with SYBR-Green Master Mix (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2020Quote: ... HEK 293 cells were plated onto 6-well plates (2.5-4.5 × 105 cells per well) in 3 ml of MEM (Life Technologies) with 10% FBS (Life Technologies ...
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bioRxiv - Neuroscience 2020Quote: ... Dissociated hippocampal or cortical neurons were plated at 17,000 cells/well (96-well plate) or 1,000,000 cells/well (6-well plate) in neuron media (Neurobasal medium (ThermoFisher 21103049) supplemented with B27 (ThermoFisher 17504044) ...
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bioRxiv - Neuroscience 2021Quote: ... Retinas were then dissected in DPBS and placed on a culture insert (Millicell) in a 6-well plate (Falcon) containing 1.3ml of equilibrated medium: DMEM (supplemented with glutaMAX; Gibco) with 10% FBS (Sigma ...
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bioRxiv - Neuroscience 2021Quote: ... 0.6 µg KV3.1b plasmid and 0.1 µg eGPF were transfected into HEK-293 cells using Lipofectamine 2000 (Invitrogen Corporation), and recordings were performed after 3 days of incubation at 37 °C ...
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bioRxiv - Neuroscience 2021Quote: DRGN and retinal cells were washed with PBS and total RNA (n = 6-9 wells/treatment) was extracted using Trizol reagent according to the manufacturer’s instructions (Invitrogen). Appropriate primers were used to validate each gene of interest using the specific primers (Table 1 ...
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bioRxiv - Neuroscience 2021Quote: ... The nucleofected cells were plated on a well of a 6 well plate coated with Geltrex matrix (Life Technologies) and transitioned to Essential 8 media (Life Technologies)1 ...
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bioRxiv - Molecular Biology 2019Quote: ... After approximately 6 hours the cells were washed with phosphate-buffered saline (PBS) and detached with TrypLE (ThermoFisher Scientific). Then ...
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bioRxiv - Cell Biology 2021Quote: 300,000 cells/well were seeded into duplicate wells of 6-well plates (Thermo Fisher Scientific, Cat# 07-200-83) in 2 mL medium ...
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bioRxiv - Cell Biology 2021Quote: ... embryos at the 3-6 somite stage were embedded oriented laterally in 1% low-melt agarose (Invitrogen, Carlsbad, CA) and imaged under bright field (to determine yolk elongation by taking major and minor axis measurements ...
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bioRxiv - Cancer Biology 2019Quote: ... whose NBD-amino group is placed at carbon 6 and can therefore not be phosphorylated by HKs34 (Thermo Fisher). The cells were then washed with ice-cold phosphate-buffered saline (PBS ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Chromosomes were counterstained for 20 minutes with 1 μg/ml DAPI (4’,6-diamidino-2-phenylindole; ThermoFisher Scientific, D3571) in 2x SSC ...
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bioRxiv - Cell Biology 2021Quote: ... Plasmids were stably transfected into Fischer Rat Thyroid (FRT) cells in 6-well plates using lipofectamine LTX (Thermo Fisher) and OptiMEM (Thermo Fisher ...
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bioRxiv - Systems Biology 2021Quote: The qPCR was conducted in duplicate using 384-well plates on a QuantStudio™ 6 cycler (Thermo Fisher Scientific). The reactions were performed using unlabeled primers and SYBR Green DNA binding dye (Applied Biosystems ...
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bioRxiv - Plant Biology 2020Quote: ... The qPCR was performed by QuantStudio™ 6 Flex Real-Time PCR machine (Applied Biosystems, Foster City, CA, USA). Elongation Factor 1α (CqEF1α)(XM_021888526.1 ...
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bioRxiv - Plant Biology 2021Quote: ... The PCR amplification was performed on QuantStudio 6 Flex Real-Time PCR Systems (Applied Biosystems, Foster City, CA, USA) with the following conditions ...
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bioRxiv - Plant Biology 2021Quote: ... Ribosome was depleted in 6 µg of RNA from each pool using the Dynabeads mRNA purification kit (Ambion, Invitrogen) following the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2021Quote: ... Ribosome was depleted in 6 µg of RNA from each pool using the Dynabeads mRNA purification kit (Ambion, Invitrogen) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... Reactions were incubated at 37°C for 4 hours and run on a 6% TBE-Urea gel (Invitrogen, EC6265BOX). Bands of the appropriate size were excised ...
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bioRxiv - Neuroscience 2020Quote: Mouse midbrains (n = 5-6 per group per age) were collected and RNA was isolated using TRIzol Reagent (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: SARS-CoV-2 (438-516) S-RBD((HiS)6 and biotynilated human ACE2 have been purchased from Fisher Scientific (respective references 16534204 and 16545164) ...
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bioRxiv - Microbiology 2021Quote: ... Samples were then washed twice with PBS and stained with 4′,6-diamidino-2-phenylindole (DAPI, Thermo Scientific #D1306) diluted 1:200 in 0.5% BSA in PBS ...
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bioRxiv - Molecular Biology 2021Quote: Transient expression of DNA plasmids was carried out in 6 well plates by reverse transfection using Lipofectamine LTX (Invitrogen). Briefly ...
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bioRxiv - Microbiology 2020Quote: The lipophilic fluorescent membrane probe 1-(4-Trimethylammoniumphenyl)-6-Phenyl-1,3,5-Hexatriene p-Toluenesulfonate (TMA-DPH) (Themo Fisher Scientific) was used to assess EV associated lipids ...
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bioRxiv - Cancer Biology 2020Quote: ... Then the membrane inserts were placed into 6-well plates containing 1.5 mL maintenance medium consisting of F12/DMEM (Gibco) supplemented with N-2 Supplement (Gibco ...
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bioRxiv - Biochemistry 2020Quote: ... 7.5 μl of 6 μM samples were separated by SDS-PAGE (NuPAGE 4-12% Bis-Tris Gel, Life Technologies) or native gels (NativePAGE 4-16% Bis-Tris Gel ...
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bioRxiv - Cancer Biology 2020Quote: ... and 0.5 μg pMD2.G were transfected into HEK293T cells in 6-well plates by Lipofectamine 2000 Transfection Reagent (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... The volume of siRNA for a final concentration of 15 nM per well was mixed with 6 μL of HiPerfect in 100 μL of Dulbecco’s modified Eagle’s medium (DMEM, Gibco, USA), incubated for 10 min and added to cells in media without antibiotics ...
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bioRxiv - Cell Biology 2020Quote: ... and the lineage-positive cells were depleted using a magnetic particle concentrator according to the manufacturer’s instructions (Dynal MPC-6, Invitrogen). To isolate the LSK/LT-HSC fraction by FACS ...
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bioRxiv - Biophysics 2021Quote: ... 5 mM DTT) and then mixed with 50 mM (final 6 mM) disuccinimidyl dibutyric urea (DSBU) (Thermo Fisher Scientific). Crosslinking reaction was incubated on ice for 2 hours and then was quenched by adding 1M (final 50 mM ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were incubated with lentiviral supernatant and 3 days later selection begun with 6 μg/mL puromycin (Thermo Fisher), which was maintained during all subsequent culturing.
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bioRxiv - Cell Biology 2020Quote: INS-1 832/13 cells were grown in 35-mm dishes and transfected at 50-60% confluency with FRET-based biosensors (2.5 μg plasmid DNA) using Lipofectamine 2000 (6 μl) (Invitrogen). 24 hours post-transfection cells were plated in μ-slide 8 well glass bottom chamber (Ibidi ...
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bioRxiv - Neuroscience 2021Quote: ... specifically designed primers (appropriate miRCURY LNA miRNA PCR assays) and QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems). Relative miRNA expression levels between control and mutant cells were quantified using ΔΔCT Method ...
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bioRxiv - Neuroscience 2022Quote: HEK293T cells (ATCC CRL-3216) were transfected in a 6-well plate with 3μg/well of DNA with Lipofectamine 2000 (ThermoFisher) in accordance with the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: ... 75 µg lysate and 50 µg media samples were proteolysed and labeled with TMT 6-plex according to the manufacturer’s protocol (ThermoFisher). Briefly ...
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bioRxiv - Bioengineering 2022Quote: ... cells were rinsed in DPBS and incubated with 2 μg/ml DAPI (4′,6-diamidino-2-phenylindole, 62248; ThermoFisher) and 1:500 Alexa Fluor Plus 647 conjugated goat anti-rabbit secondary antibodies (A32733 ...