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Citations for Thermo Fisher :
2901 - 2950 of 4608 citations for 6 HYDROXY CHRYSENE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pathology 2023Quote: ... Cells were spun down at 350g for 6 minutes and were re-suspended with 4mL of 0.05% Trypsin (ThermoFisher) and incubated at 37°C for 10 minutes ...
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bioRxiv - Neuroscience 2023Quote: ... The RT-qPCR was performed using the Promega® 2x Master Mix and the QuantStudio™ 6 (Applied Biosystems) for 40 cycles ...
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bioRxiv - Neuroscience 2023Quote: ... The parental hiPS cell line (8858-3) was maintained in 6-well plates using StemFlex medium (Life Technologies, A3349401). Cas9 ...
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bioRxiv - Immunology 2023Quote: ... The paraffin blocks were sliced into 5 to 6 μm sections using a Microm HM 325 microtome (Thermo Scientific), and the sections were stained with Hematoxylin and Eosin (H&E) ...
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bioRxiv - Molecular Biology 2023Quote: Total RNA from mating wild-type and EMA2 KO cells were isolated at 6 hpm using TRIzol reagent (Invitrogen). The RNA was treated with Turbo DNase (Ambion ...
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bioRxiv - Cancer Biology 2023Quote: ... was transduced by lentivirus and successfully transduced cells were selected by 6 μg/ml blasticidin (R21001; Thermo Fisher Scientific) for 1-2 weeks ...
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bioRxiv - Neuroscience 2023Quote: ... Organ of Corti explants were isolated from C57BL/6 mice at postnatal day 4 in cold Leibovitz’s L-15 medium (cat. # 21083027, Gibco) and held in place by two glass fibers glued to the bottom of a Petri dish ...
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bioRxiv - Cell Biology 2023Quote: Interleukin-6 levels in supernatants were determined using commercially available human IL-6 ELISA kits according to the manufacturer’s protocol (Invitrogen). A detailed description of the procedure are reported in Supporting methods ...
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bioRxiv - Molecular Biology 2023Quote: ... pH 6) at 40°C with vigorous shaking at 1400rpm for 30 minutes using a thermal mixer (Thermo Scientific). The eluted DNA and previous input samples were then amplified using the PCR Primer Mix 3 for Illumina and the VAHTS HiFi Amplification Mix 2 (ND607 ...
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bioRxiv - Genetics 2024Quote: Total RNA was extracted from samples containing 6 OD600 units of mid-log phase cells using Trizol (Invitrogen #15596026) and chloroform ...
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bioRxiv - Cell Biology 2024Quote: ... Quantitative PCR (qPCR) was conducted on a QuantStudio™ 6 Flex Real-Time PCR System (Applied Biosystems, CA, USA). Relative mRNA levels were determined using the Comparative Ct (△△CT ...
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bioRxiv - Cell Biology 2024Quote: ... coverslips with the gel attached were transferred to a well of a 6-well plate with 1 ml of digestion solution (0.5% Triton X100, 1 mM EDTA pH 8 (AM9260G, Ambion), 50 mM Tris pH 8 (AM9855G ...
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bioRxiv - Cell Biology 2024Quote: ... An overnight incubation with specific antibodies (P16, IL-6 or phospho-H2AX (S139) and cTNT (Invitrogen, #MA-5-12960)) was followed by 2 hours of incubation with secondary antibody conjugated with Alexa Fluor 488 ...
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bioRxiv - Genomics 2024Quote: ... and the relative expression was determined by the 2-ΔΔCt method on a QuantStudio 6 qPCR machine (Thermo Fisher). The levels of mRNAs were normalized to human ACTB.
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bioRxiv - Neuroscience 2024Quote: Brains were dissected from 2-day old adults in PBS and incubated in 1.6 × 10-6 M acridine orange (ThermoFisher) for 5 min at room temperature ...
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bioRxiv - Plant Biology 2024Quote: ... equal amounts of RNA were prepared in equal volumes (6 µL) using UltraPure DNase/RNase-free distilled water (Invitrogen). RNA samples were denatured at 95 °C for 3 min and placed on ice immediately to prevent the formation of secondary structures ...
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bioRxiv - Biophysics 2024Quote: ... USA) dissolved in ethanol or 1μL 2.5mg/mL N,N-Dimethyl-6-dodecanoyl-2-naphthylamine (LAURDAN; Thermo Fisher, USA) dissolved in dimethyl sulfoxide to label the EVs ...
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bioRxiv - Cancer Biology 2024Quote: ... typically 1.5 μg of plasmid was reverse transfected into 300,000 293T cells in a well of a 6-well plate using OptiMEM (Gibco) and FuGENE 6 (Promega) ...
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bioRxiv - Biophysics 2024Quote: ... at a 6:1:2 ratio for each virus using the Bac-to-Bac baculovirus expression system (Thermo Fisher). Cell cultures were grown in ESF 921 medium (Expression Systems ...
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bioRxiv - Biochemistry 2024Quote: ... followed by a 6× His-tag was cloned into the BamHI and XbaI sites of the pcDNA 3.1 vector (#V79020, Invitrogen) containing the vascular endothelial growth factor receptor 1 (VEGFR ...
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bioRxiv - Microbiology 2024Quote: ... sg.ORF7a) and genomic RNA (NSP3 region; g.NSP3) (90) on a QuantStudio 6 Pro Real-Time PCR System (Applied Biosystems). Relative mRNA expression was normalized to the levels of GAPDH and expressed relative to the values for control cells using the ΔΔCt method.
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bioRxiv - Molecular Biology 2024Quote: WT and TRBP−/− HeLa cells were plated at 4 x 106 cells / dish into a 9-cm dish and transfected with 6 µg/dish poly(I:C) using Lipofectamine 2000 (Invitrogen). At 0 ...
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bioRxiv - Molecular Biology 2024Quote: ... HEPA1-6 were lysed in RIPA buffer and loaded on a 4-15% gradient polyacrylamide gel (Thermo Fisher, NP0335). Protein transfer was carried out using the iBlot system (Thermo Fisher ...
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bioRxiv - Cell Biology 2024Quote: ... a 6-well was coated with 1 mL of a 1:200 solution of either Geltrex (Gibco™ A1413202) or Cultrex (Bio-Techne 3445-005-01 ...
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bioRxiv - Cell Biology 2024Quote: ... The reaction was incubated for 15 minutes at room temperature and then separated on a 6% DNA retardation gel in 0.5X TBE running buffer (ThermoFisher). The gel was imaged with a SapphireTM multimode imager in the Keck Biophysics facility at Northwestern University.
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bioRxiv - Physiology 2024Quote: ... Cells were grown overnight in glass slides and loaded with 10-6 mol.L-1 4,5-diaminofluorescein-diacetate (DAF-2DA; Molecular Probes) for 30 minutes ...
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bioRxiv - Pathology 2024Quote: ... IRB number: VGHUST105-G7-6-1) were cultivated in Dulbecco’s modified Eagle’s medium (Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 10% heat-inactivated fetal bovine serum ...
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bioRxiv - Microbiology 2020Quote: ... Minimum triplicate reactions per sample were performed using a QuantStudio 6 flex qPCR machine (Applied Biosystems, Foster City, CA, USA) according to the following thermal cycling conditions ...
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bioRxiv - Cancer Biology 2021Quote: Cancer cells were transfected at ∼30% confluency in 6-well plates with 5nM control or target siRNA using Lipofectamine RNAiMAX (Invitrogen) according to the instructions of the manufacturer ...
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bioRxiv - Microbiology 2020Quote: ... T175 flasks containing intracellular parasite grown in normal media were treated with auxin for 6 hours before sample collection and addition of Trizol (Invitrogen). RNA was extracted as per manufacturer instruction ...
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bioRxiv - Cell Biology 2020Quote: ... Rat RFL-6 fibroblasts were purchased from the American Type Culture Collection (ATCC) and cultured in Ham’s F-12K medium (Thermo Fisher) supplemented with 20% FBS as previously described (Qiang et al. ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were washed twice with 1X PBS and stained with either DAPI (4’,6-diamidino-2-phenylindole, from Life Technologies), for the visualization of the nucleus ...
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bioRxiv - Cell Biology 2020Quote: We seeded 2×105 cells / well into 6 well dishes and replaced media with 5% KSR (Knockout Serum Replacement) (Gibco) containing media the next day ...
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bioRxiv - Cell Biology 2020Quote: ... collected into a new tube and stained with 4′,6-diamidino-2-phenylindole (DAPI) (∼5 µg/ml, Molecular Probes # D1306). Round coverslips (Ø12 mm ...
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bioRxiv - Cell Biology 2020Quote: ... Quantitative real-time PCR was run on a ViiA 7 or QuantStudio 6 Flex PCR thermal cycler (Thermo Fisher Scientific) using SensiMix™ SYBR® No-ROX Kit (Bioline ...
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bioRxiv - Immunology 2021Quote: ... monocyte-derived DCs that were transfected with hACE2 were seeded at 0.5×106 cells/mL in a 6-well plate and transfection was performed with Lipofectamine LTX and PLUS reagents (Invitrogen) according to the manufacturer’s instructions for primary cells ...
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bioRxiv - Genetics 2021Quote: Live myofibers in the 6-well plate were stained with 2 µL of 5mg/mL of Hoechst (Molecular Probes, H1399) in 2 mL of 1X PBS for 5 minutes in a 37 °C with 5% CO2 incubator ...
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bioRxiv - Developmental Biology 2021Quote: ... For experiments glass-bottomed imaging dishes were exposed to deep UV light for 6 minutes and coated with Poly-D-Lysine (ThermoFisher) for a minimum of 20 minutes ...
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bioRxiv - Microbiology 2020Quote: ... aureus overnight cultures were diluted 1:200 into 3ml of fresh TSB or TSBg per well of a Nunc MicroWell 6-well plate (no. 140675; ThermoFisher) and incubated statically at 37°C for 6 hours ...
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bioRxiv - Molecular Biology 2020Quote: ... Samples were separated by electrophoresis on a 6% bis-Acrylamide (19:1) gel that was stained overnight with 1X SYBR green I nucleic acid gel stain (Invitrogen). The gel was imaged and quantified using an AlphaImagerHP (Alpha Innotech) ...
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bioRxiv - Microbiology 2019Quote: N2a cells were transiently transfected at ≈70% confluence in a 6-well plate by adding per well 10 μL Lipofectamine 2000 (Invitrogen) together with 3 μg of the respective pcDNA3.1 plasmid derivative ...
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bioRxiv - Molecular Biology 2021Quote: ... Transfections were carried out in a 12-well plate or 6-well plate using Lipofectamine 3000 (Invitrogen; Life Technologies Inc.) following the reverse transfection protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... Transfections were carried out in a 12-well plate or 6-well plate using Lipofectamine 3000 (Invitrogen; Life Technologies Inc.) following the reverse transfection protocol ...
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bioRxiv - Microbiology 2021Quote: ... Slides were washed for 3 × 5 mins in PBS after each antibody step and in the penultimate wash 2μg/ml 4’,6-diamidino-2-phenylindole (DAPI) (Molecular Probes) was added ...
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bioRxiv - Developmental Biology 2021Quote: Total RNA was isolated from the 60 hpa tissues (n=6) from each group using TRIzol reagent (Applied Biosystems, USA). One microgram of total RNA was reverse-transcribed to cDNA using a one-step cDNA Synthesis Kit (Applied Biosystems ...
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bioRxiv - Immunology 2022Quote: Splenocytes from C57BL/6 mice were disassociated into a single cell suspension and labeled with 1μM CellTrace Far Red (ThermoFisher Sci). Targets were then divided into two populations ...
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bioRxiv - Cell Biology 2022Quote: ... 1 μg of plasmid was used to transfect 750,000 cells in each well of a 6-well plate using Lipofectamine 2000 (5 μL; Thermo Fisher) in OptiMEM (400 μL ...
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bioRxiv - Bioengineering 2022Quote: hPSC-CMs were maintained in Matrigel pre-coated 6-well plates in RPMI 1640 medium (Gibco REF 11875-093 500ml) with B-27 supplement (Gibco REF 17504-044 10ml) ...
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bioRxiv - Microbiology 2022Quote: ... cells seeded in a 6-well plate were transfected with 1 μg of pPRIME-FLuc plasmid using Lipofectamine 2000 (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: NIH-3T3 cells were seeded on 6-well plates and allowed to grow to 70% confluency at 37°C and 5% CO2 in DMEM (Gibco) supplemented with 10% FBS (Life Technologies) ...