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Citations for Thermo Fisher :
2451 - 2500 of 4608 citations for 6 HYDROXY CHRYSENE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2021Quote: ... hiPSC-IMR90-1 cells were cultured on a Matrigel-coated 6-well plate with Essential 8 medium (Gibco). The medium was changed daily ...
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bioRxiv - Genetics 2021Quote: ... The relative mtDNA copy number was assessed using QuantStudio 6 Flex Real-Time PCR system (Thermo Fisher Scientific). The mtDNA and the nuclear-encoded housekeeping gene 18S were amplified using primer sequences ...
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bioRxiv - Genetics 2020Quote: ... Cells were passaged 1:2-1:6 every 2-4 days by being rinsed once with DPBS (Gibco) and dissociated using 0.5 mM EDTA (75 µl/cm2 ...
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bioRxiv - Immunology 2021Quote: ... were cocultured at 1:1 ratio for 6 days with anti-CD3/CD28–coated beads (Dynabeads, Life Technologies) at 1 bead ...
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bioRxiv - Physiology 2020Quote: ... DAPI (4’,6-Diamidino-2-Phenylindole Dihydrochloride; 1:2000 diluted in PBTD; Thermo Fisher Scientific, Stockholm, Sweden; 62248) was added for 3 minutes followed by washing in PBTD for 25 minutes and mounting with ProLong gold antifade reagent (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2019Quote: ... the medium in the 6 well plates was replaced with pure OMEM without phenol (#11058-21, ThermoFisher Scientific) by adding 1ml per well ...
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bioRxiv - Cancer Biology 2021Quote: Cells were stimulated for a total of 6 hours with human T-activator anti-CD3/CD28 Dynabeads (ThermoFisher) stimulation with GolgiStop solution being added for a total of 5 hours block intra-cellular protein transport (BD Bioscience) ...
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bioRxiv - Physiology 2021Quote: ... 6-10 microliters of a mixture of green fluorescent particles (Thermo Scientific, 1.05 g/cm3, fluorescence 589 nm) were injected using a 2.5 μm Hamilton glass syringe with a pulled borosilicate capillary tube (Fig.2A) ...
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bioRxiv - Developmental Biology 2021Quote: ... After 30 min incubation with 100 ng/mL 4’,6-diamidino-2-phenylindole (DAPI; Thermo Fisher Scientific D1306), specimens were washed three times with PBS−/− ...
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bioRxiv - Cell Biology 2021Quote: ... supplemented with 1% penicillin–streptomycin in 6-well plates coated with truncated recombinant human vitronectin (Thermo Fisher Scientific). The iPSC line was previously assessed for pluripotency and normal karyotype.33 For differentiation ...
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bioRxiv - Synthetic Biology 2021Quote: ... and cultured for 6 days in maintenance medium comprising Dulbecco’s minimum essential media DMEM/F12 (77.5 % v/v, Gibco), Knockout Serum Replacement (20 % v/v ...
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bioRxiv - Neuroscience 2021Quote: ... slices were counterstained with 4’,6-diamidine-2’-phenylindole 502 dihydrochloride (DAPI, D3571, Molecular Probes; dilution 1:1000) for 5 minutes followed by 2 minutes wash in PBS ...
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bioRxiv - Neuroscience 2021Quote: ... Isolated MEFs were plated in 2 wells of a 6-well plate and cultured overnight in DMEM (Gibco) supplemented with 10% FBS ...
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bioRxiv - Genomics 2021Quote: ... qPCR was performed on Viia 6/7 Real time PCR system using SYBR Green master mix (Life Technologies). The primers used are listed in Supplementary table 1 ...
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bioRxiv - Cell Biology 2021Quote: ... Cell pellets were then resuspended and plated into one well of a 6 well plate (Fisher Scientific 1483211). Cells were left to sit for 5 days at 37°C and 5% CO2 after initial plating and were only fed once after three days by adding an additional volume of FCM ...
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bioRxiv - Cell Biology 2021Quote: ... These experiments were carried out with the QuantStudio 6 and 7 Flex Real-Time PCR Systems (Applied Biosystems). Expression of circRNAs was calculated relative to the housekeeping genes 18S or ACTB ...
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bioRxiv - Biochemistry 2021Quote: ... quantitative PCR was performed using a Quant-Studio 6 Flex Real-Time PCR instrument (Thermo Scientific, Waltham, MA). For sample preparation ...
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bioRxiv - Cell Biology 2020Quote: ... 1.8 mM CaCl2, 6 mM NaHCO3, 5.5 mM D-glucose, 25 mM HEPES, pH 7.4 supplemented with Gibco MEM Amino Acids and MEM Non-Essential Amino Acids solution ...
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bioRxiv - Neuroscience 2021Quote: ... Cells fixed in methanol were incubated with 4’,6-Diamidino-2-pheny-lindoldihydrochloride (DAPI; Thermo Fisher Scientific #D1306) for 10 minutes and subsequently imaged at 6 locations per condition using a Leica DMi8 microscope ...
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bioRxiv - Immunology 2020Quote: ... For poly(I:C) stimulation cells were transfected in 6-well plates with 10 μg polyI:C using Lipofectamine 2000 (Invitrogen) for 2 h before imaging ...
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bioRxiv - Immunology 2020Quote: ... or SYBR Green chemistry and reactions were run on a RT-PCR system (QuantiStudio 6 Flex, Applied Biosystems). Gene expression is displayed as fold increase over antibiotic-pre-treated ...
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bioRxiv - Cell Biology 2021Quote: ... Slides were mounted using ProLong™ Gold Antifade Mountant with 4’,6-diamidino-2-phenylindole (DAPI) (ThermoFisher Scientific). Imaging was performed using a Leica TCS SP8 confocal microscope.
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bioRxiv - Cell Biology 2021Quote: ... and selected antibiotics according to the respective expression constructs: blasticidin S (6 μg mL−1, Thermo Fisher Scientific), puromycin (0.5 μg mL−1 ...
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bioRxiv - Neuroscience 2021Quote: ... incubated for 10 min in PBS/10 ug/mL 4’-6-diamidino-2-phenylindole (DAPI, Thermo Fisher Scientific) to stain nuclei ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were seeded in 6-well plates and transfected the following day using lipofectamine 3000 (Thermo Fisher Scientific). 20 mM oleic acid complexed with 2.5 mM BSA were added to cells 16 h post transfection ...
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bioRxiv - Neuroscience 2022Quote: Immunofluorescence was performed using glial fibrillary acidic protein (GFAP) and 4′,6-diamidino-2-phenylindole (DAPI) solution (ThermoFisher). GFAP primary and secondaries were diluted to a ratio of 1:1000 and 5:1000 ...
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bioRxiv - Cell Biology 2022Quote: ... Nuclei were counterstained using 4′,6-diamidin-2-phenylindol (DAPI, 1:1000, 7 min; D1306, Thermo Fisher Scientific). Washing steps were performed with PBS ...
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bioRxiv - Bioengineering 2022Quote: ... the cell nuclei were stained with 1 μg/mL 4’,6-diamidino-2-phenylindole (DAPI, ThermoFisher Scientific #62248) in DPBS for 15 min at room temperature ...
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bioRxiv - Bioengineering 2022Quote: ... Nuclei and F-actin were counterstained with 4′,6-diamidino-2-phenylindole dihydrochloride (DAPI, 300 nM, D1306, ThermoFisher) and phalloidin-AlexaFluor 647 (1:100 ...
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bioRxiv - Bioengineering 2022Quote: CD19+ NALM-6 cells (B cell acute lymphoblastic leukemia) were stained with Cell Trace Violet (Thermo Fisher Scientific) and primary human CD8+ T cells were stained with either Cell Trace Yellow (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2022Quote: ... rested for 6 hours and stimulated overnight in 1 ml of complete medium (RPMI 1640 with Glutamax, Gibco) containing 10% fetal bovine serum (FBS ...
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bioRxiv - Immunology 2022Quote: ... CRP and IL-6 were measured in UCB plasma using a high-sensitivity ELISA (Life Technologies, Carlsbad CA) per the manufacturer’s instructions.
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bioRxiv - Immunology 2022Quote: ... 70% confluent HEK293T cells in 6 well plates were transfected in Opti-MEM reduced serum medium (Thermo Fisher) with 250 ng of 8 plasmids ...
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bioRxiv - Developmental Biology 2022Quote: ... and pGL4.74-Renilla luciferase plasmids in a ratio of 0.5:1:0.1 (4 μg/6-well dishes) using Lipofectamine LTX PLUS (Invitrogen). The luciferase activity was assayed using the Dual-Luciferase Reporter Assay Kit (Promega) ...
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bioRxiv - Microbiology 2022Quote: ... Membranes were next treated with 1: 1000 dilution of primary antibody (6-His tag mouse anti-tag, Invitrogen) for 1 hr at RT ...
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bioRxiv - Microbiology 2022Quote: ... Each of the three biological replicates were quantified in technical duplicates using Quantstudio 6 Flex (Applied Biosystems #4485694) incubating at 50°C for 10 min ...
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bioRxiv - Immunology 2022Quote: ... The slices were mounted with ProLong Diamond Antifade Mountant with 4’,6-diamino-2-phenylindole (DAPI) (ThermoFisher Scientific). Fluorescent images were analyzed with a Nikon confocal microscope (Nikon A1RMP ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... ΔFZD1-10 or ΔLRP5/6 cells were transiently transfected in suspension using Lipofectamine 2000 (Thermo Fisher Scientific 11668). An approximately 80-90% confluent T-75 flask was re-suspended in 20 ml yielding a suspension with 4-5×105 of cells ml-1 ...
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bioRxiv - Neuroscience 2023Quote: ... Slices were either washed using DAPI (4’,6-Diamidino-2-Phenylindole, Dihydrochloride; 1:10000; Thermo Fisher Scientific, MA) made up in di H2O to stain for nuclei before being mounted or cover-slipped with ProLong Gold mounting medium with DAPI (Fisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... 1.5μg Cas9/gRNA containing plasmid and 1.5μg repair template were co-transfected into 6-well dishes using Lipofectamine 3000 (Invitrogen) with a 3:1 transfection reagent to DNA ratio for 48 hours ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... dishes were mounted with ProLong Diamond mounting medium containing 4’,6-diamidino-2-phenylindole (DAPI) (Invitrogen, Waltham, MA), and imaged using a Zeiss LSM 780 laser confocal microscope equipped with a C-Apochromat 40x/1.2W objective.
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bioRxiv - Cancer Biology 2024Quote: ... cells-were transfected in 6-cm plates 24 hours after second siRNA transfection with Lipofectamine 2000 (ThermoFisher Scientific) and 2 μg of pCMV6M-Pak1-WT (Addgene ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were mixed and dispensed at 120k cells/per well (6 well size) coated with 1x Geltrex (ThermoFisher). Cells were incubated at 37 °C incubator overnight ...
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bioRxiv - Neuroscience 2022Quote: ... hiPS cells were maintained in 6-well plates coated with recombinant human vitronectin (VTN-N, Life Technologies, A14700) in Essential 8 medium (Life technologies ...
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bioRxiv - Neuroscience 2022Quote: ... mice were sacrificed 6-8 days after the last CDSD session and RNA was extracted with TriReagent (Ambion). Sequencing libraries were prepared with ScriptSeq v2 RNA-seq library preparation kit (Epicentre ...
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bioRxiv - Cell Biology 2022Quote: ... were used for qPCR in a QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems, Foster City, CA) according to the manufacturer’s protocol for 40 cycles ...
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bioRxiv - Physiology 2022Quote: ... RT-qPCR was performed using a QuantStudio 6 Flex Real-Time PCR System (Thermo Fisher Scientific, Waltham MA) with a 384-well platform ...
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bioRxiv - Cell Biology 2022Quote: ... For the bulk endocytosis experiments using the 4-[6-[4-(diethylamino)phenyl]-1,3,5-hexatrien-1-yl]-1-[3-(triethylammonio)propyl]-pyridiniumbromide dye (FM 4-64, Invitrogen), the cells were prepared as previously described [41] ...
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bioRxiv - Genomics 2022Quote: ... 10-20×10^6 adherent cells were first removed from the plate using StemPro Accutase (ThermoFisher, A11105-01) or TrypLE Express (ThermoFisher ...
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bioRxiv - Cell Biology 2022Quote: ... NIH/3T3 cells were plated on 12×12 mm coverslips in 6-well plates (ThermoFisher Scientific, cat# 140675). The next day cells were pre-treated with 2 mM Thymidine (Sigma-Aldrich ...