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Citations for Thermo Fisher :
2601 - 2650 of 10000+ citations for Recombinant Human Neuropilin 1 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... Antibodies used for human cells are mouse anti-human CD3 conjugated to APC (Thermo Fisher, 47-0036-42), mouse anti-human CD8 conjugated to FITC (BD Biosciences ...
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bioRxiv - Cell Biology 2022Quote: Human cerebral organoids are generated from human induced pluripotent stem cells (iPSCs) which were purchased from GibcoTM (Gibco by Thermofisher ...
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bioRxiv - Cancer Biology 2020Quote: ... anti-human TNF-α (hTNF-α)-PE and anti-human IFN-ɣ (hIFN-ɣ)-PECy7 were from ThermoFisher. Anti-human CD4 (hCD4)-BV711 was from Biolegend.
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bioRxiv - Immunology 2020Quote: ... using a secondary antibody recognizing all human immunoglobulin isotypes (goat anti-human IgG H+L-HRP, Invitrogen/ThermoFisher) [12,13] ...
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bioRxiv - Immunology 2021Quote: ... which contains the gene expression profile of human microglia using a human gene ST 2.0 Microarray Chip (Affymetrix). The human microglia were polarized to either pro-inflammatory (M1 ...
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bioRxiv - Immunology 2020Quote: ... using a secondary antibody recognizing all human immunoglobulin isotypes (goat anti-human IgG H+L-HRP, Invitrogen/ThermoFisher) [12,13] ...
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bioRxiv - Neuroscience 2024Quote: ... The concentrations of human α-synuclein were measured using a human α-syn-specific ELISA (ThermoFisher Scientific #KHB0061) according to the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2024Quote: ... Human full-coding KATNA1 cDNA was isolated from a human fetal brain Marathon-ready cDNA collection (Clontech, Invitrogen) with the following forward and reverse primers ...
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bioRxiv - Immunology 2023Quote: Total human IgG levels were determined following the manufacturer’s instructions in the Human IgG Total ELISA Kit (Invitrogen).
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bioRxiv - Bioengineering 2023Quote: ... primary human T cells were thawed and activated with anti-CD3/CD28 Human T-Expander Dynabeads (Thermo Fisher) at a 3:1 bead to cell ratio ...
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bioRxiv - Synthetic Biology 2023Quote: ... primary human T cells were thawed and activated with anti-CD3/CD28 Human T-Expander Dynabeads (Thermo Fisher) at a 3:1 bead to cell ratio ...
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bioRxiv - Cell Biology 2020Quote: All cell lines were cultured in DMEM media supplemented with sodium pyruvate and 10% Hi-FBS (Life Technologies). To express protein constructs in living cells ...
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bioRxiv - Molecular Biology 2020Quote: ... 5% CO2 for 24 h before transient transfection with plasmids encoding Affimer-His constructs using Lipofectamine 2000 (ThermoFisher), as per the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... HEL and K562 cells were all from American Type Culture Collection (ATCC) and cultured in RPMI with 10% HI FBS except for K562 (grown in IMDM (Gibco, #21980-032) with 10% HI FBS ...
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bioRxiv - Molecular Biology 2019Quote: Wild-type and indicated mutants of MmEsco1 were cloned into pEF6/Myc-His B vector (Thermo Fisher Scientific) containing C-terminal myc and His tags ...
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Dimeric prion protein ligand activates Adgrg6 but does not rescue myelinopathy of PrP-deficient micebioRxiv - Neuroscience 2020Quote: ... Briefly, sheep-anti mouse IgG paramagnetic beads (Dynal, 11201D) were coupled with anti-His mAb (Invitrogen, 37-2900) in coating buffer (PBS plus 0.1% immunoglobulin-free BSA ...
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bioRxiv - Cell Biology 2021Quote: ... rKLK10 with a 6x C-terminal His tag was affinity purified using HisPur Ni-NTA Resin (Thermo Scientific) per the manufacturer’s instruction (Figure S16 ...
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bioRxiv - Molecular Biology 2020Quote: ... RNA-protein complexes were purified with Pierce(tm) His Protein Interaction Pull-Down Kit (Thermo Fisher Scientific, #21277) with the following modifications ...
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bioRxiv - Biochemistry 2021Quote: ... The resulting cyclic peptide-mRNA:cDNA library was counter-selected 3 times against His Pull-Down Dynabeads (Thermo Fisher), and affinity selected against 200 nM bead-immobilised Retromer for 30 min ...
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bioRxiv - Biophysics 2021Quote: Detergent extracted His-MBP-NavSp1 was applied to a 40 mL POROS MC 20 Ni2+ column (Applied Biosystems) equilibrated with buffer A (200 mM NaCl ...
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bioRxiv - Cell Biology 2022Quote: ... Sequencing runs were conducted on Proton sequencer using the PI™ Hi-Q™ Sequencing 200 Kit (Thermofisher) following the recommendations of the manufacturer ...
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bioRxiv - Biochemistry 2019Quote: ... Each protein was first purified by metal affinity chromatography using 2ml of His purTM cobalt column (Thermo Scientific). The tag was removed by cleavage with TEV protease followed by purification on a second cobalt affinity chromatography ...
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bioRxiv - Molecular Biology 2019Quote: ... and cDNA was immediately synthetized using Hi-capacity RNA-to-DNA™ kit (Applied Biosystems™, ref. 4388950) both according to manufacturers instructions ...
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bioRxiv - Microbiology 2020Quote: ... and sequenced using the Ion PGM Hi-Q View sequencing kit (Life Technologies, Grand Island, NY, United States) with the Ion PGM™ System technology ...
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bioRxiv - Molecular Biology 2022Quote: ... and sub cloned in BamHI and SalI site of pCDNA3.1/myc-His(A) mammalian expression vector (Invitrogen, USA) to generate pCDNA3.1-PAX9-myc-His(A ...
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bioRxiv - Immunology 2020Quote: ... were coated at room temperature for 8 hours with 1ug/mL PolyRab anti-His antibody (ThermoFisher, PA1-983B), followed by overnight blocking with blocking buffer containing 1x PBS ...
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bioRxiv - Immunology 2020Quote: ... were coated at room temperature for 8 hours with 1ug/mL PolyRab anti-His antibody (ThermoFisher, PA1-983B), followed by overnight blocking with blocking buffer containing 1x PBS ...
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bioRxiv - Immunology 2020Quote: ... were coated at room temperature for 8 hours with 1ug/mL PolyRab anti-His antibody (ThermoFisher, PA1-983B), followed by overnight blocking with blocking buffer containing 1x PBS ...
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bioRxiv - Immunology 2020Quote: The PCR products obtained by SGA were cloned into the commercial directional vector pcDNA3.1D/V5-His-TOPO according to the manufacturer’s instructions (Invitrogen). The correct pcDNA3.1-Env plasmid used to produce wild type pdsudoviruses were selected by sequencing ...
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Neutralizing activity of broadly neutralizing anti-HIV-1 antibodies against primary African isolatesbioRxiv - Immunology 2020Quote: ... The cytomegalovirus (CMV) promoter was amplified by PCR from the pcDNA 3.1D/V5-His-TOPO plasmid (Life Technologies) with forward primer 5’-AGTAATCAATTACGGGGTCATTAGTTCAT-3’ and reverse primer 5’ CATAGGAGATGCCTAAGCCGGTGGAGCTCTGCTTATATAGACCTC-3’ ...
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bioRxiv - Immunology 2020Quote: ... were coated at room temperature for 8 hours with 1ug/mL PolyRab anti-His antibody (ThermoFisher, PA1-983B), followed by overnight blocking with blocking buffer containing 1x PBS ...
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bioRxiv - Immunology 2020Quote: ... were coated at room temperature for 8 hours with 1ug/mL PolyRab anti-His antibody (ThermoFisher, PA1-983B), followed by overnight blocking with blocking buffer containing 1x PBS ...
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bioRxiv - Genetics 2020Quote: ... Purified N-HIS-FAM19A5 was then digested overnight at 30°C with AcTEV protease (Thermo Fisher Scientific, USA) to remove the His-tag ...
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bioRxiv - Immunology 2022Quote: ... Wildtype and mutant coding sequences were transferred to the pEF1/V5-His vector (cat. no. V920; Life Technologies) for overexpression ...
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bioRxiv - Microbiology 2024Quote: ... we followed the vendor supplied protocol of the His-Pur Ni-NTA Purification Kit (3 ml, Thermo Fisher). The flow through ...
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bioRxiv - Molecular Biology 2024Quote: ... The washed membrane was then incubated overnight at 5°C with a 6x-His-Tag monoclonal antibody (Invitrogen) diluted 2500-fold in PBS-Tween ...
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bioRxiv - Immunology 2023Quote: HEK293 cells were transfected with pcTBK1-His or pcGSK3B-Flag plasmid using Lipofectamine 2000 (Invitrogen, Waltham, MA, US) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... Reverse transcription for other transcripts was performed using the hi-capacity cDNA reverse transcription kit (Applied Biosystems #4368814) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... We diluted anti-stem Abs (starting at the lowest concentration, which yielded measurable HI) in C1q-DPBS (Gibco) (220 nM) ...
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bioRxiv - Cell Biology 2022Quote: His-CLASP1 protein was expressed in baculovirus-infected Sf9 insect cells using the Bac-to-Bac system (Invitrogen). After the first amplification ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were transfected with a pMT Puro His-PC-SNAP-Elp3 vector using ExpiFectamine™ Sf(A389l5, Gibco) using manufacturer’s instruction ...
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bioRxiv - Cell Biology 2023Quote: ... entry clones containing individual TSWV proteins were recombined with a pIB/V5-His destination vector (Thermo Fisher Scientific) containing either GFP or mRFP in both amino and carboxy terminal fusions when possible ...
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bioRxiv - Microbiology 2023Quote: ... TCID50) were calculated by IFA using HIS α NDV and Alexa FluorTM 488 α-rabbit secondary antibody (Invitrogen) in two independent experiments.
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bioRxiv - Microbiology 2024Quote: The HylB expression vector pAOJ66 was generated by amplifying the ef0818 gene with primers AOJ_651 and AOJ_652 and cloning into NcoI-HF/ApaI digested pBAD/myc his A (Invitrogen). The vector was propagated in E ...
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bioRxiv - Developmental Biology 2024Quote: Drosophila S2 cells were transfected with the indicated plasmids or blank pMT-V5-His vector (Invitrogen cat#V412020) using Effectene transfection reagent (Qiagen cat#301427) ...
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bioRxiv - Genetics 2024Quote: ... and hybridizing the Hi-C libraries with custom probes before enrichment via Dynabeads MyOne Streptavidin Beads T1 (ThermoFisher). After library enrichment ...
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bioRxiv - Plant Biology 2024Quote: Cloning and expression of His-mVenus-SUF4 was based on the Bac-to-Bac Baculovirus Expression system (ThermoFisher). The control pFastBac-mVenus and pFastBac-mVenus-SUF4 isoform 1 plasmids were obtained from Dr ...
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bioRxiv - Cell Biology 2022Quote: ... cells were co-stained with recombinant Cholera toxin subunit B conjugated to Fluor488 (Invitrogen C34775) at 1 μg/mL for less than 1 minute prior to fixing and staining for NINJ1 as described above ...
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bioRxiv - Physiology 2019Quote: Recombinant adenoviruses were generated using the pAd/CMV/V5-DEST Gateway recombination system (Life Technologies) after cloning the full-length cDNA into the pENTR vector ...
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bioRxiv - Biochemistry 2019Quote: ... The resulting antiserum was affinity purified using recombinant CTLMA2 immobilized on Aminolink resin (Thermo Fisher). Western blotting was performed with affinity purified anti-CTLMA2 ...