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Citations for Thermo Fisher :
2851 - 2900 of 10000+ citations for Recombinant Human Neuropilin 1 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... cardiomyocytes were incubated with the secondary antibody diluted in a blocking buffer solution: goat anti-Human Alexa Fluor 647 (1:400; A-21445 - Invitrogen) and goat anti-Mouse 594 (1:400 ...
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bioRxiv - Cancer Biology 2020Quote: ... HDL was labeled with Alexa568 by incubating 4.5 mg Human HDL in 90 μl 1 M sodium bicarbonate with Alexa Fluor 568 succinimidyl ester (A20003, Thermo Fisher) for 1 hr at room temperature (8:1 dye:estimated apoA1 mole ratio) ...
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bioRxiv - Immunology 2021Quote: ... Primary antibodies were diluted 1:100 in PBS-T as follows: single labeling: CD14 (mouse anti-human, 14-149-82, Invitrogen), CD56 (mouse anti-human ...
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bioRxiv - Microbiology 2021Quote: ... PMA differentiated THP-1 cells were treated with 10 μg/mL mouse monoclonal anti-human IFNAR2 (ThermoFisher Scientific, MMHAR-2), or human IgG (Invitrogen ...
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bioRxiv - Cell Biology 2021Quote: Total RNA was isolated from human cells (1 x 106 cells) using the TRIZOL reagent according to the manufacturer’s instructions (ThermoFisher Scientific). RNA integrity for each sample was confirmed with by Nano Drop ...
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bioRxiv - Immunology 2020Quote: ... and human fibroblasts (HF) clone EF-1-F (sourced from Doorbar lab, University of Cambridge) were grown in MEM (Gibco) supplemented with 10 % FBS ...
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bioRxiv - Microbiology 2021Quote: ... HEK-293T overexpressing the human ACE2 were kindly provided by Integral Molecular Company and maintained in DMEM with 1 μg/mL of puromycin (Invitrogen). TMPRSS2 human plasmid (Origene ...
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bioRxiv - Microbiology 2021Quote: ... on HEK-293T overexpressing the human ACE2 and maintained in the previously described media containing 1 mg/mL of geneticin (Invitrogen) to obtain TMPRSS2/ACE2 HEK-293T cells ...
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bioRxiv - Cell Biology 2021Quote: ... RWPE-1 (human normal prostate epithelial cells, CRL-11609, ATCC) cells were cultured in Keratinocyte Serum Free Medium (17005042, Gibco) supplemented with 0.05 mg/ml bovine pituitary extract ...
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bioRxiv - Cell Biology 2021Quote: ... hiPSC-CMs were washed 3 times with PBS 1X and incubated with the secondary antibodies diluted in blocking solution: goat anti-Human Alexa Fluor 647 (1:400; A-21445 - Invitrogen) and goat anti-Mouse Alexa Fluor 488 (1:400 ...
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bioRxiv - Biophysics 2020Quote: Expression was conducted according to the manual of 1-Step Human High-Yield Mini in vitro translation (IVT) kit (Product No. 88891X) distributed by ThermoFisher Scientific (Pierce Biotechnology ...
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bioRxiv - Developmental Biology 2022Quote: ... These zona-free eggs were incubated with 200 nM Fc-fusion proteins in Biggers-Whitten-Whittingham (BWW) medium for 1 h and then stained with goat anti-human IgG Fc antibody DyLight 488 (Invitrogen) at a dilution of 1:50 for 1 h at 37 °C ...
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bioRxiv - Molecular Biology 2022Quote: ... Cells were washed 3 times with PBS and incubated for 1 hour with Alexa 488 nm or Alexa 594 nm conjugated secondary antibodies specific for human and rat IgG (Invitrogen) diluted 1:2,000 in 3% BSA in PBS and containing 1 μg/ml DAPI ...
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bioRxiv - Cancer Biology 2022Quote: ... the ST1 cells were transfected with NICD pCMV Neo/intracellular domain of human Notch1 (NIC-1) plasmid using Lipofectamine 200 (Invitrogen), and then were harvested 48 hours after transfection for western blot and in vitro angiogenesis assay.
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bioRxiv - Biochemistry 2021Quote: ... The slide was washed thrice with PBS-T for 1 minute and further incubated with goat anti-human IgG (Fc)-Cy5 (Invitrogen) antibodies at 1:2500 dilution ...
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bioRxiv - Immunology 2019Quote: ... T cells were stimulated for 2 days at a starting density of approximately 1 million cells per mL of media with anti-human CD3/CD28 Dynabeads (ThermoFisher), at a bead to cell ratio of 1:1 ...
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bioRxiv - Bioengineering 2019Quote: ... rinsed and re-suspended in 100 μL of 25 μg mL−1 mouse anti-human CD3-AF700 (56-0037-42, ThermoFisher), CD4-PE-Cy7 (25-0048-42 ...
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bioRxiv - Bioengineering 2019Quote: ... was diluted 1:200 in PBS/5% FCS/0.1% Tween® 20 and primary MMP1 antibody (mouse anti-human; Invitrogen Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2019Quote: ... 1 × 106 human cells in 35 mm dish per sample were washed with 1 × PBS and lysed with 600 μl DNAzol (Invitrogen), followed by precipitation with 300 μl 200 proof ethanol ...
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bioRxiv - Molecular Biology 2021Quote: ... Human globin mRNA was depleted from all samples except from samples #1 and #2 using the GLOBINclear kit (ThermoFisher Scientific). The quality of the RNA was assessed using the Agilent 6000 Pico kit with the Bioanalyzer 2100 (Agilent ...
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bioRxiv - Immunology 2020Quote: ... Digested sera was probed with Rabbit anti-Human IgG-HRP H+L Secondary Antibody (ThermoFisher Scientific Catalog #A18903, 1:10,000), Rabbit anti-Human IgG Fc HRP Secondary Antibody (ThermoFisher Scientific Catalog #31423 ...
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bioRxiv - Evolutionary Biology 2020Quote: ... In order to generate the construct for inducible ZEB2 expression from the AAVS1 site the coding sequence of the human ZEB2 transcript variant 1 (NM_014795.3) was purchased as an ultimate ORF clone in the pENTRY221 (Thermo Scientific, #IOH53645). The ZEB2 ORF was amplified with primers ZEB2_IndOE_F & _R and tagged with GFP-Flag by Gibson assembly into the pT2-CAG-MCS-2A-Puro plasmid linearized by restriction digestion with EcoRI (NEB ...
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bioRxiv - Cancer Biology 2020Quote: ... CPA and CPD cells were cultured in Keratinocyte-SFM supplemented with 2.5 μg prequalified human Epidermal Growth Factor 1-53 (EGF) (Life technologies), 25 mg Bovine Pituitary Extract (BPE ...
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bioRxiv - Immunology 2020Quote: ... Wells were washed three times with 1xDPBS followed by a one hour incubation with horse radish peroxidase-conjugated goat anti-human total Ig at a dilution of 1:6000 (Invitrogen). Wells were then washed four times with 1xDPBS followed by a 5-15 minute incubation with TMB substrate (SeraCare) ...
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bioRxiv - Microbiology 2021Quote: ... on HEK-293T overexpressing the human ACE2 and maintained in the previously described media containing 1 mg/ml of geneticin (Invitrogen) to obtain TMPRSS2/ACE2 HEK-293T cells.
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bioRxiv - Immunology 2021Quote: ... 30,000 purified cells were stimulated in round-bottom 96-well plates with human anti-CD3/anti-CD28 T cell expander beads (1 bead/cell; ThermoFisher) in complete media containing 10 U/mL rhIL-2 with or without 10 or 20 uM 6-(4-Chlorophenyl)imidazo[2,1-b][1,3]thiazole-5-carbaldehyde O-(3,4-dichlorobenzyl)oxime (CITCO ...
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bioRxiv - Cell Biology 2021Quote: ... For spindle length and chromosome alignment analyses the following secondary antibodies were used at 1:500 dilution: Goat anti-Human IgG conjugated to Alexa Fluor 488 (A11013; Invitrogen), Goat anti-Mouse IgG conjugated to Alexa Fluor 647 (A21236 ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were then incubated with corresponding secondary antibodies for 1 hr at room temperature: goat anti-human-Alexa-488 and goat anti-mouse-Alexa-488 (Invitrogen) for 293T internalization studies and goat anti-rabbit-Alexa-488 and goat anti-human-Alexa-555 (Invitrogen ...
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The MuSK-BMP pathway maintains myofiber size in slow muscle through regulation of Akt-mTOR signalingbioRxiv - Cell Biology 2022Quote: ... Serum was diluted 1:1000 in 1/10 block overnight at 4°C and detected using goat anti-human IgG Alexa Fluor 555 (Invitrogen) diluted 1:1000 and counterstained with AlexaFluor488-conjugated α-bungarotoxin (Thermo Fisher ...
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bioRxiv - Immunology 2022Quote: ... freshly isolated or cryopreserved human PBMC or purified CD8+ T-cells (1×106) were cultured in complete culture medium (RPMI 1640 (Gibco) supplemented with 10% FCS ...
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bioRxiv - Immunology 2022Quote: ... then co-cultured at the indicated ratios and activated with Dynabeads Human T-Expander (1 bead:16 CD3+ T cells; Gibco) for 4 days ...
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bioRxiv - Molecular Biology 2024Quote: ... cDNA encoding full-length human ADNP2 (amino acid residues 1–1131) was PCR amplified and cloned into a pFast-Bac-derived vector (Invitrogen) in frame with an N-terminal His6-tag ...
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bioRxiv - Neuroscience 2024Quote: ... or Dynabeads™ Human T-Activator CD3/CD28 for T Cell Expansion and Activation (1:30 bead:T-cell ratio; Gibco) or (2 ...
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bioRxiv - Cell Biology 2024Quote: ... the mucosal layer was removed from an approximately 1 cm3 fresh section of human colonic tissue and washed twice with 1X Dulbecco’s phosphate-buffered saline (DPBS, ThermoFisher, 14040133). The mucosa was transferred to a 5-ml conical tube containing 0.5 ml crypt isolation buffer (2 mM EDTA ...
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bioRxiv - Immunology 2024Quote: ... Bound mAb was detected using goat anti-human (H+L)-HRP (1:1000 dilution) (Thermo Fisher Scientific, Waltham, MA, USA). In the case of His-tag detection ...
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bioRxiv - Microbiology 2024Quote: ... Membranes were blocked for 1h at room temperature with blocking buffer (3% skim milk powder dissolved in tris-buffered saline supplemented with 0.1% Tween-20 [TBS-T]) and then probed with CV3-25 mAb (1ug/mL) followed by HRP-conjugated anti-human IgG (1:3000, Invitrogen). HRP enzyme activity was determined after the addition of a 1:1 mix of Western Lightning oxidizing and luminol reagents (PerkinElmer Life Sciences ...
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bioRxiv - Microbiology 2023Quote: ... β-actin expression was measured using an anti-human β-actin horseradish peroxidase-conjugated mAb (1:25000, Life Technologies, USA) as the loading control ...
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bioRxiv - Microbiology 2023Quote: ... a separate aliquot of each sample during each immunoblot was probed with a monoclonal mouse anti-human GAPDH antibody (1:500 dilution; #43700, Invitrogen) and a peroxidase-conjugated goat anti-mouse secondary antibody (1:5,000 dilution ...
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bioRxiv - Cell Biology 2023Quote: ... The cells were incubated overnight at 4ºC with mouse anti-human αSMA (Cat. 14-9760-82, Invitrogen: 1:500 dilution) and goat anti-human CD144 (Cat ...
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bioRxiv - Bioengineering 2023Quote: ... The membranes were then incubated with a 1:10,000 dilution of goat anti-human Fc HRP secondary antibody (Invitrogen, A18817) in PBST for 1 h ...
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bioRxiv - Biochemistry 2023Quote: ... Plates were slapped dry and washed 4X in TBST followed by addition of 50 μL 1:5000 Goat anti-Human IgG Fc Secondary Antibody with HRP (Invitrogen) for one hour at 37°C ...
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bioRxiv - Biophysics 2023Quote: ... Clathrin Heavy Chain siRNA (Human CLTC, sequence: GGUUGCUCUUGUUACG, ID: s475) and Negative Control#1 siRNA Silencer Select were purchased from ThermoFisher Scientific.
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bioRxiv - Bioengineering 2023Quote: ... TJC4) was detected using a 1:500 dilution of goat anti-human Alexa Fluor 488 or Alexa Fluor 647 (Invitrogen). Secondary antibodies were incubated for 15 min at 4°C in the dark ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were labelled with phycoerythrin (PE)-conjugated anti-human-HLA-ABC (clone W6/32, Invitrogen 12-9983-42; 1:20), biotin-conjugated anti-mouse-H-2Kb (clone AF6-88.5 ...
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bioRxiv - Immunology 2023Quote: ... The wells were washed 5x and incubated with 100 µl/well with an HRP-conjugated anti-human IgG Fc antibody (1:10000 dilution, Invitrogen) for 1 hour ...
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bioRxiv - Microbiology 2023Quote: ... bacteria were incubated with 1:1000 diluted PE-conjugated F(ab’)2-anti-human-kappa-light-chain (Invitrogen, PA1-74408) in PBS/ 0.1% BSA (20 min ...
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bioRxiv - Bioengineering 2023Quote: ... Cells were then stained for 2 hr at room temperature with the following antibodies at the manufacturer-recommended dilutions in 1× TFP Perm/Wash buffer: APC- eFluor780 anti-human CD3 (clone UCHT1, Invitrogen); PerCP-Cy5.5 anti-human CD4 (clone SK3 ...
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bioRxiv - Bioengineering 2023Quote: ... The brain sections were then incubated with goat anti-mouse Alexa Fluor 488 and goat anti-human Alexa Fluor 647 (1:500, Invitrogen). Following secondary staining ...
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bioRxiv - Immunology 2023Quote: ... Purified T cells were activated for 1 to 3 days with Human T-Activator anti-CD3/CD28 dynabeads at a 1:2 bead-to-cell ratio (Gibco, Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2023Quote: Undifferentiated H1 (WA01; XY) human embryonic stem cells (hESCs) were maintained on Geltrex coated plates (1:100 in DMEM/F12; Gibco) in StemFlex Medium (Gibco ...