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Citations for Thermo Fisher :
2451 - 2500 of 10000+ citations for Recombinant Human Neuropilin 1 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2023Quote: ... RNA extracts were stabilized by adding the RNAseOUT recombinant ribonuclease inhibitor (Thermo Fisher Scientific), before freezing at −80°C until further analysis.
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bioRxiv - Biophysics 2023Quote: Recombinant baculovirus was generated by transfecting Sf9 cells with bacmid using Cellfectin II (ThermoFisher). Baculoviruses were harvested from Sf9 cell media by filtering through a 0.22 µm filter and amplified three times before using for cell transduction ...
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bioRxiv - Biochemistry 2022Quote: ... Recombinant protein was resolved by NuPAGE™ 4-12% Bis-Tris Gel (Invitrogen; NP0322BOX) to determine identity and purity ...
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bioRxiv - Biochemistry 2023Quote: The following recombinant proteins were purchased from commercial sources: alkaline phosphatase (Thermo Scientific #EF0651), Antarctic phosphatase (New England BioLabs ...
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bioRxiv - Cancer Biology 2023Quote: ... the epidermis was separated from the dermis and incubated in TrypLETM recombinant enzyme (ThermoFisher) for 1h and subsequently dissociated mechanically with a pipette tip ...
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bioRxiv - Immunology 2023Quote: ... and recombinant Cas9 protein via electroporation using the Neon transfection system (Thermo Fisher Scientific). The following day ...
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bioRxiv - Immunology 2023Quote: ... The recombinant bacmid DNA was generated using the Bac-to-Bac system (Life Technologies). Baculoviruses were generated by transfecting purified bacmid DNAs into Sf9 cells using FuGENE HD (Promega) ...
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bioRxiv - Plant Biology 2023Quote: ... The recombinant proteins were purified using HisPur™ Ni-NTA resin (Thermo Fisher Scientific) and amylose resin (NEB) ...
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bioRxiv - Developmental Biology 2023Quote: ... Recombinant anti-GFP nanobody was conjugated to epoxy-activated magnetic Dynabeads M270 (Life Technologies) using 10 μg recombinant protein/1 mg Dynabeads ...
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bioRxiv - Microbiology 2023Quote: ... and STRN3 were carried out utilising immobilised recombinant protein A/G resin (Thermo Scientific). Cell pellets were harvested and processed as described in the EGFP co-immunoprecipitation section ...
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bioRxiv - Immunology 2023Quote: ... Recombinant antigen was immobilized on N-hydroxysuccinimide (NHS)–activated agarose resins (Thermo Fisher, 26197) by overnight rotation at 4°C ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were stained with anti-CD64 (CD64 Recombinant Rabbit Monoclonal Antibody, Thermofisher, # MA5-29706) at a dilution of 1:400 in 1% BSA in PBS and incubated at 4°C overnight in the dark overnight ...
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bioRxiv - Immunology 2023Quote: ... recombinant bacmid DNA was generated using the Bac-to-Bac system (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... Recombinant RBD (Arg319 – Phe541) of the same strain was obtained from Invitrogen (# RP 87678) and used as an immunogen without conjugation with KLH and used for screening assays ...
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bioRxiv - Neuroscience 2024Quote: ... An injection of Cholera Toxin Subunit B (recombinant) conjugated to Alexa Fluor 647 (ThermoFisher) was made in the striatum of the same hemisphere (+1.2mm AP ...
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bioRxiv - Microbiology 2024Quote: Recombinant ACE2 protein was coated on 96-well NUNC Maxisorp plates (Thermo Fisher Scientific) at 100 ng per well overnight at 4°C ...
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bioRxiv - Immunology 2024Quote: ... Recombinant bacmid DNA was generated using the Bac-to-Bac system (Thermo Fisher Scientific), following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... Chromatin was cleared with recombinant protein G– conjugated agarose beads (Invitrogen, cat# 15920-010) and subsequently immunoprecipitated with H3K27ac antibody (Active Motif ...
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bioRxiv - Genetics 2021Quote: Human neural stem cells derived from H9 (WA09) human embryonic stem cells were purchased from Thermo Fisher. The cells were cultured in complete StemPro NSC SFM (Thermo Fisher ...
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bioRxiv - Neuroscience 2020Quote: NSChIPS (Human Neural Stem Cells derived from the human induced pluripotent stem (iPS) cell line) (ThermoFisher, #A3890101)
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bioRxiv - Genomics 2019Quote: ... a mixture of 23 normal human tissues (including brain) and First Choice Human Brain reference RNA (Ambion), a pool of normal human brain tissues (labeled B) ...
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bioRxiv - Immunology 2022Quote: ... The human codon-optimized Bcl6-P2A-human codon-optimized Bcl-xL insert was synthesized using GeneArt (ThermoFisher) and cloned into pLZRS-IRES-GFP ...
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bioRxiv - Immunology 2020Quote: ... Fresh primary human T cells were isolated and cultured with anti-human CD3/CD28 beads (Dynabeads, ThermoFisher), 500U/mL IL-2 (Proleukin ...
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bioRxiv - Molecular Biology 2021Quote: Human adipose-derived stem cells (ASCs) and human microvascular endothelial cells (HMVEC) were purchased from Thermo Fisher Scientific (Carlsbad ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: Primary human epidermal melanocytes (MC) and primary human epidermal keratinocytes (KC) were obtained from Invitrogen (NY, USA). MC was cultured in Medium
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bioRxiv - Molecular Biology 2022Quote: ... Human TNF-α Ultrasensitive ELISA Kit (Invitrogen,KHC3014 [96 tests] and Human IFNα ELISA Kit [Invitrogen,BMS216TEN]) were used for cytokine quantification.
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bioRxiv - Neuroscience 2023Quote: ... Human fibroblasts and Human embryonic kidney (HEK) 293T cells were cultured in DMEM (1g/L glucose) (GIBCO) supplemented with 10% Fetal Calf Serum (FCS) ...
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bioRxiv - Neuroscience 2023Quote: ... Human and non-human primate OBs were sectioned following embedding in optimal cutting temperature (O.C.T.) compound (ThermoFisher). One human non-synucleinopathy case was obtained from banner health embedded in paraffin wax (Specimen details see Table 1 and SI Appendix Table S1) ...
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bioRxiv - Immunology 2023Quote: Primary human T cells were pre-activated with DynabeadsTM human T-Activator CD3/CD28 beads (Gibco, #11131D) at a 1:1 ratio of cells:beads with 100 IU/ml IL-2 (Chiron #53905-991-01 ...
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bioRxiv - Molecular Biology 2023Quote: ... T cells were isolated from human PBMCs with an Untouched Human T-cell Isolation Kit (Invitrogen, USA) and activated with Dynabeads Human T Activator CD3/CD28 (Gibco ...
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bioRxiv - Physiology 2024Quote: Human: Total RNA was extracted from human RV tissue using TRIzol (Ambion by Life Technologies, Ref#15596018). cDNA was prepared using qScript Flex cDNA Synthesis Kit from (Quanta Bio ...
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bioRxiv - Physiology 2024Quote: Human: Total RNA was extracted from human RV tissue using TRIzol (Ambion by Life Technologies, Ref#15596018). cDNA was prepared using qScript Flex cDNA Synthesis Kit from (Quanta Bio ...
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bioRxiv - Neuroscience 2024Quote: ELISAs for human Aβ40 and human Aβ42 were carried out using commercial kits (Aβ40: Invitrogen KHB3481; Aβ42: Invitrogen KHB3441) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... Proliferation was triggered by the stimulation with Dynabeads Human T-Activator CD3/CD28 (ratio 1:2, Thermo Fisher Scientific). The arginase inhibitor OAT-1746 (1500 nM) ...
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bioRxiv - Immunology 2020Quote: ... The human monocytic THP-1 cell line was maintained in RPMI-1640 (made inhouse, RPMI 1640 powder (Life Technologies), 23.8mM Sodium Bicarbonate (NaHCO3 ...
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bioRxiv - Immunology 2021Quote: ... NAFs/CAFs ± SI were added to wells of lymphocytes at a concentration of 1×104 cells/100μl (ratio of 1:10 NAFs/CAFs:lymphocytes) of human complete MSC medium (RPMI 1640 medium (ThermoFisher Scientific) supplemented with 10% heat-inactivated FBS ...
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bioRxiv - Biochemistry 2020Quote: Cas9 was produced by IVT using either a continuous exchange 1-Step Human Coupled IVT Kit (Thermo Fisher Scientific) or a PURExpress bacterial IVT kit (New England Biolabs) ...
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bioRxiv - Microbiology 2021Quote: ... Cells were then washed once with PBS and incubated with 1 ug/ml anti-human (Alexa Fluor 647; Invitrogen) secondary Abs and the viability dye AquaVivid (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2020Quote: ... Samples were further diluted 1:100 in standard diluent and Aβ levels were measured with the human Aβ1–42 ELISA kit (Invitrogen) following the manufacturer’s instructions.
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bioRxiv - Immunology 2021Quote: ... The human myelomonocytic leukemia cell line THP-1 was propagated in RPMI 1640 medium (GIBCO® Life Technologies™) supplemented with 10% fetal calf serum (FCS ...
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bioRxiv - Neuroscience 2019Quote: ... for 1 h at room temperature and then exposed to antibodies specific for human LMP7 (ThermoFisher Scientific; MA5-15890) or PRDX6 (Abcam ...
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bioRxiv - Cell Biology 2021Quote: Total RNA (1 μg) preparations were hybridized on the PrimeView Human Gene Expression Array (Affymetrix, Thermo Fisher Scientific, USA) at the core facility Biomedizinisches Forschungszentrum (BMFZ ...
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bioRxiv - Cell Biology 2021Quote: Total RNA (1 μg) preparations were hybridized on the PrimeView Human Gene Expression Array (Affymetrix, Thermo Fisher Scientific, USA) at the core facility Biomedizinisches Forschungszentrum (BMFZ ...
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bioRxiv - Cell Biology 2021Quote: ... The following secondary antibodies were used at 1:200 for IF experiments: Anti-human-Alexa-633 (Life Technologies #A21091), anti-mouse-Alexa-488 (Life Technologies #A11029) ...
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bioRxiv - Molecular Biology 2022Quote: Human Embryonic Kidney (HEK293T) cells were cultured in high glucose DMEM with 10% FBS and 1% Penicillin/Streptomycin (Gibco) in a 37°C incubator with 5% CO2 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: 1 mL of human plasma was incubated with 0.5 mL of Invitrogen total exosome isolation reagent (Thermo Fisher Scientific) overnight at 4°C and the day after was centrifuged at 10,000 x g for 1h at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... Alpha-actinin-1-M-sstFRET containing the sstFRET module inserted between 300aa and 301aa within the first spectrin repeat of human alpha-actinin-1 (P12814.2) was in the neomyocin selectable pcDNA3.1 expression vector (Invitrogen/ThermoFisher). Actinin-C-sstFRET containing the sstFRET module added to the C-terminus of human alpha-actinin-1 (P12814.2 ...
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bioRxiv - Microbiology 2021Quote: ... falciparum NF54 parasites were cultivated at 4% hematocrit in human B+ blood in RPMI-HEPES supplemented with 0.25% Albumax 1 (Invitrogen) and human donor plasma type B+ ...
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bioRxiv - Synthetic Biology 2020Quote: ... and 1 day prior to spinfection cells were activated using Human T-activator CD3/CD28 Dynabeads (Thermo Fisher, 11131D) and cultured in 200U/ml IL-2 ...
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bioRxiv - Bioengineering 2021Quote: ... Another subset of slides was labeled with Alexa 568 conjugated anti-human IgG antibody (1:800, ThermoFisher, A-21090) to detect the Fc portion of the EphA4-Fc chimera peptide.