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Citations for Thermo Fisher :
2601 - 2650 of 10000+ citations for Killer cell immunoglobulin like receptor 2DL3 KIR2DL3 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... A HeLa cell lysate-based Kit (1-Step Human Coupled IVT Kit—DNA, 88881, Life Technologies) enabled in vitro translation of these constructs ...
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bioRxiv - Bioengineering 2024Quote: ... and resuspended in complete human embryonic stem cell (hESC) medium consisting of DMEM/F12 (11320033, ThermoFisher), 20% (v/v ...
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bioRxiv - Biochemistry 2023Quote: Human MCF10A breast epithelial cells (ATCC CRL-10317) were cultured in DMEM/F-12 (Gibco 11330032) with 5% horse serum (Gibco 16050122) ...
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bioRxiv - Neuroscience 2024Quote: Human primary Müller cells (P3) after siRNA treatments were incubated with JC-1 dye (#T3168, Invitrogen) at a concentration of 1.0 μg/mL in DMEM at 37 °C for 30 minutes to measure the mitochondrial membrane potential ...
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bioRxiv - Immunology 2024Quote: Freshly isolated CD8+ cells were activated with Dynabeads™ Human T-Activator CD3/CD28 (Gibco™) at a cell/bead ratio of 1:0.5 for 48 h in conditioned media obtained by combining R10 medium and culture supernatants derived from either leukemic or healthy B cells (1:1 ratio) ...
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bioRxiv - Cell Biology 2024Quote: ... PC3 cells were transfected with cDNA encoding human talin-1 (NM_006289) using Lipofectamine 3000 reagent (Invitrogen) according to the manufacturer’s recommendation ...
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bioRxiv - Cell Biology 2024Quote: ... Human MCF10A breast epithelial cells were cultured in DMEM/F12 (DMEM/Nutrient Mixture F-12, Gibco) supplemented with 5% fetal horse serum (Gibco) ...
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bioRxiv - Biochemistry 2024Quote: ... which were co-transfected into human U251-MG glioblastoma cells (ECACC 09063001) using Lipofectamine 3000 (ThermoFisher). Transfectants were selected for three days at which point selection antibiotic was removed ...
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bioRxiv - Immunology 2024Quote: ... and containing recombinant human stem cell factor (h-SCF; Gibco, PeproTech, #300-07; 100 ng/mL) and human IL-6 (Gibco ...
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bioRxiv - Immunology 2024Quote: The human Burkitt lymphoma cell line (BJAB) was cultured in Dulbecco’s modified Eagle medium (DMEM,GIBCO) supplemented with 10% heat-inactivated fetal calf serum ...
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bioRxiv - Cell Biology 2021Quote: ... anti-human IgG-Alexa647 and anti-human IgG-Alexa568 were obtained from Invitrogen, anti-acetylated tubulin (T7451 ...
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bioRxiv - Cell Biology 2020Quote: ... and human GAPDH and human KIF18A Taqman probes and primers (Thermo Fisher Scientific) were used for reverse transcription and qRT-PCR ...
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bioRxiv - Neuroscience 2020Quote: ... and subjected to human total tau ELISA (human tau: # KHB0042, Thermo Fisher Scientific) according to manufacturer’s instructions.
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bioRxiv - Molecular Biology 2020Quote: ... we introduced the hAsCas12a gene into the human HEK 293 T-REx cell line nuclear genome (Flp-In ™ T-REx ™ −293 Cell Line, Invitrogen) allowing controlled expression by the tet-on system ...
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bioRxiv - Biochemistry 2022Quote: ... Resting CD4+ T cells were purified from bulk PBMCs by using Dynabeads™ Untouched™ Human CD4 T Cells Kit (ThermoFisher Scientific).
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... maintenance culture was carried out for all cell lines including human ES cells using albumin-free Essential 8 Medium (Thermo Fisher Scientific) in six-well feeder-free culture dishes coated with 5 μg/mL vitronectin (VTN-N ...
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bioRxiv - Biochemistry 2021Quote: Resting CD4+ T cells were purified from bulk PBMCs by using Dynabeads™ Untouched™ Human CD4 T Cells Kit (ThermoFisher Scientific). Selected CD4+ T cells were activated by Dynabeads™ Human T-Activator CD3/CD28 kit (ThermoFisher Scientific ...
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bioRxiv - Genetics 2020Quote: Human embryonic stem cells and human induced pluripotent stem cells (hESCs and iPSCs, Table S1) were grown using StemFlex™ Medium (A3349401, Gibco™) supplemented with Normocin™ Antimicrobial Reagent (Invivogen ...
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bioRxiv - Immunology 2021Quote: ... 2 million cells were activated with dynabeads human T activator CD3/CD28 at a 1:1 bead to cell ratio (Thermo Fisher Scientific), Staphylococcal enterotoxin B (SEB ...
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bioRxiv - Molecular Biology 2022Quote: ... human embryonic kidney 293T cells were seeded at a density of 13×106 cells per 15cm plate in DMEM (Thermo Fisher, 11965092) with 10% fetal bovine serum (Gibco ...
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bioRxiv - Synthetic Biology 2023Quote: Mixed CD4+ and CD8+ primary T cells were isolated with the Dynabeads™ Human T-Activator CD3/CD28 for T Cell Expansion and Activation (Thermo Fisher) from healthy donor whole blood obtained from the Stanford Blood Center (Stanford ...
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bioRxiv - Immunology 2024Quote: ... we co-transfected 3×106 3-day activated CD8+T cells with 150 pmol/106 cells of human TSP-4-specific siRNAs (#s14100, Thermo Fisher Scientific) and 1.5 μg/106 of the pMax-TSP-1-GFPSpark construct ...
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bioRxiv - Neuroscience 2020Quote: Short hairpin RNAs against the mu and delta receptors were designed using BLOCK-IT RNAi Designer software (Invitrogen, USA). The sequences of the 21-nt fragments complementary to the target mRNAs were GCTGCCCTTTCAGAGTGTTAA (Oprm1-1) ...
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bioRxiv - Neuroscience 2021Quote: ... The cDNA encoding the short transcriptional variant of human DA D2 receptor with an N-terminal FLAG tag (SF-D2R-S) was inserted into mammalian expression vector pcDNA 3.1(+) (Invitrogen)62 ...
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bioRxiv - Cell Biology 2020Quote: ... the surface receptors were labeled with 0.133 mg/ml of EZ-Link Sulfo-NHS-SS-Biotin (Thermo scientific, 21331) in PBS at 4 °C for 30 min ...
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bioRxiv - Microbiology 2019Quote: ... and baby hamster kidney 21 cells expressing the MHV receptor (BHK-R) (32) were maintained at 37°C in Dulbecco’s Modified Eagle Medium (DMEM, Gibco) supplemented with 10% serum (HyClone FetalClone II ...
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bioRxiv - Immunology 2022Quote: ... This master mix was added to premade 96 well TaqMan Array plates with chemokine/chemokine receptor primers (Thermo Fisher, Mouse Chemokines & Receptors Array plate ...
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bioRxiv - Neuroscience 2019Quote: ... a cDNA encoding this receptor was cloned into the eukaryotic expression vector pcDNA 3.1(+) (Invitrogen; Cat. No. V790-20). To facilitate expression of the cloned receptor ...
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bioRxiv - Immunology 2020Quote: ... pH 7.2) resuspended and incubated for 30 min in FACS buffer supplemented with Fc gamma receptor CD16/CD32 antibodies (ThermoFisher). Cells were then incubated with Alexa fluor 488-conjugated antibodies against F4/80 or rat IgG2a kappa isotype control ...
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bioRxiv - Immunology 2020Quote: ... Reverse sICs were generated from these receptor-specific antibodies using goat-anti-mouse IgG F(ab)2 fragments (Invitrogen) in a 1:1 ratio ...
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bioRxiv - Microbiology 2021Quote: Vero cells that stably express the canine receptor CD150 (Vero-cCD150) were grown in advanced Dulbecco’s modified Eagle medium (DMEM; Gibco) supplemented with 10% (V/V ...
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bioRxiv - Neuroscience 2023Quote: ... The following primary antibodies were used: rabbit polyclonal anti-GPR10 receptor (1:200; PA5-29809 Thermo Scientific, Paisley, UK); rabbit polyclonal anti-NPFF2 receptor (1:200 ...
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bioRxiv - Neuroscience 2024Quote: HEK293 cells were seeded in 6-well plates and transfected with 0.25μg LgBiT-miniG (miniGs or miniGsq45) or LgBiT-β-arrestin240 and 0.25μg SmBiT-tagged receptor plasmids using 3µL Lipofectamine 2000 (Thermo Fisher). 24 hours after transfection ...
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bioRxiv - Cell Biology 2024Quote: ... The mouse monoclonal transferrin receptor antibody (H68.4; 1:200 dilution) and rabbit polyclonal Rab11 antibody (71-5300; 1:50 dilution) were from Invitrogen, while the mouse monoclonal antibody against LAMP-1 (H5G11 ...
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bioRxiv - Bioengineering 2024Quote: ... HUVEC samples were treated with an Fc receptor blocking reagent (1 test) (Thermo Fisher Scientific, Cat. # 14-9161-73) and incubated at 4°C for 15 minutes ...
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bioRxiv - Cell Biology 2020Quote: Plasmids encoding for recombinant His-tagged proteins were transformed into Escherichia coli BL21(DE3) (Invitrogen). Bacterial cultures were grown at 37°C in LB media to OD600 of 0.6-0.8 and induced with 0.5 mM IPTG at 18 °C for 16h (overnight) ...
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bioRxiv - Genomics 2020Quote: For Hi-C experiments knockdown of CLAMP was validated using the Western Breeze kit (Invitrogen). Antibodies used for detection were a previously described custom rabbit antiCLAMP (1:1,000 ...
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bioRxiv - Genomics 2020Quote: The psep-1::his-58::eGFP transgene was generated using three-site Gateway cloning (Invitrogen) in the MosSCI compatible vector pCFJ150 ...
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bioRxiv - Immunology 2021Quote: ... supplemented with 10 % heat-inactivated fetal bovine serum (HI FBS, Thermo Fisher Scientific, Loughborough, UK) and antibiotics (100 U/mL penicillin and 100 µg/mL streptomycin ...
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bioRxiv - Microbiology 2019Quote: ... A 1:150 dilution of anti-His antibody labelled with AF647 (Thermo Fisher, Waltham, MA) was then added to cells ...
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bioRxiv - Cell Biology 2019Quote: ... The His-mCherry-fusion proteins were purified using a Ni-NTA column (Thermo Fisher Scientific). The eluted proteins were dialyzed in 1 L dialyzed buffer (20 mM Tris-HCl pH 7.5 ...
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bioRxiv - Microbiology 2019Quote: ... mouse anti-6x-His epitope tag monoclonal antibody (Thermo Fisher Scientific, Cat. No. MA1-21315); LI-COR IRDye 800CW goat anti-rabbit IgG (LI-COR ...
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bioRxiv - Molecular Biology 2019Quote: ... The Jpx E1-E3 isoform was cloned into pEF1/V5-His vector (Invitrogen Cat# V92020), which contains an EF-1α promoter for mammalian expression and a T7 promoter for in vitro transcription ...
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bioRxiv - Synthetic Biology 2019Quote: ... Im7-6 was purified from IVG reactions using magnetic His-tag Dynabeads (Thermo Fisher Scientific). The IVG reactions were diluted in 90 µl of Buffer 1 (50 mM NaH2PO4 and 300 mM NaCl ...
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Genomic approach for conservation and the sustainable management of endangered species of the AmazonbioRxiv - Genomics 2020Quote: ... One microliter of amplification was mixed with 8.5 μL Hi-Di deionized formamide (Applied Biosystems), and 0.5 μL GeneScan 500 LIZ (Applied Biosystems ...
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bioRxiv - Biochemistry 2021Quote: ... Cleaved 14His-SUMO and His-Ulp1 were removed by affinity to Ni-NTA Agarose (Invitrogen). The flowthrough was diluted 1:5 with MonoQ buffer A and loaded onto a MonoQ 5/50 GL column (GE Healthcare) ...
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bioRxiv - Microbiology 2020Quote: ... under the control of an inducible pBAD promoter (pBAD/His A, Thermofisher, Catalog number 43001) (Supplementary Table 5) ...
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bioRxiv - Microbiology 2020Quote: ... The PCR products were subcloned into the pAc5.1/V5-His A vector (Invitrogen, V4110-20) with a GFP tag sequence at its 3’ end terminus ...
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bioRxiv - Microbiology 2019Quote: ... with 30% L929 supernatant and 20% fetal bovine serum (HI-FBS, Thermo Scientific, Waltham, MA) for 48 hours at 37°C under 5% CO2 ...
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bioRxiv - Biochemistry 2022Quote: His-V5-uL4-CGN mRNAs were obtained using a mMESSAGE mMACHINE T7 kit (Invitrogen #AM1344) using linear DNA templates ...