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Citations for Thermo Fisher :
2401 - 2450 of 10000+ citations for Killer cell immunoglobulin like receptor 2DL3 KIR2DL3 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2019Quote: ... MRC5 human fibroblast cell line and A375 human melanoma cell line are cultured at 37°C under 5% CO2 in DMEM medium (Life Technologies) containing 1% glutamine ...
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bioRxiv - Biochemistry 2019Quote: The human embryonic kidney (HEK) 293FT cells a high transfection efficiency isolate of the HEK293T cells were purchased from ThermoFisher (# R70007), and used without further authentication ...
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bioRxiv - Genetics 2021Quote: The human lung epithelial cell line Calu-3 (UC Berkeley Cell Culture Facility) was maintained RPMI media supplemented with Glutamax (Thermo Fisher), 10% FBS (HyClone) ...
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bioRxiv - Cell Biology 2020Quote: ... The iPSC-O cells were cultured on mouse embryonic fibroblasts (MEFs) and proliferated using a medium for human ES cells [KNOCKOUT DMEM (Gibco,10829), 15% CTS KnockOut SR XenoFree Medium (Gibco,14150) ...
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bioRxiv - Immunology 2019Quote: ... Human T-cells isolated were also used to generate T-cells blasts using the Dynabeads Human T-Activator CD3/CD28 for T-cell expansion and Activation kit (Life Technologies) as per company’s protocol ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Both of the in vitro systems depend on the application of three-dimensional embryoid body cultures derived from either mouse embryonic stem cells (mESC, ES-D3, ATCC, CRL-1934) or human episomal induced pluripotent stem cells (hiPSC, Gibco, A18945). The teratogenicity determination of the mEST is based on the half maximal inhibition of differentiation into beating cardiomyocytes (ID50 ...
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bioRxiv - Immunology 2021Quote: ... T cells were activated with Dynabeads Human T-Activator CD3/CD28 (beads/cell ratio of 1/2; Gibco, Thermo Fisher Scientific) or with 4.5 μg/mL phytohemagglutinin (PHA ...
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bioRxiv - Immunology 2021Quote: ... T cells were activated with Dynabeads Human T-Activator CD3/CD28 (beads/cell ratio of 1/2; Gibco, Thermo Fisher Scientific) or with 4.5 μg/mL phytohemagglutinin (PHA ...
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bioRxiv - Cell Biology 2022Quote: ... DNAs were amplified by PCR from human Caco2 cells or MCF7 breast cancer cells and cloned into Gateway™ expression vectors (Invitrogen) according to the manufacturer instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... and human gastrointestinal stromal tumour cells (GIST882) (National Infrastructure of Cell Line Resource, Beijing, China) were seeded in confocal dish (ThermoFisher Scientific) and grew in DMEM in 5% CO2 in a 37 °C incubator ...
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bioRxiv - Immunology 2024Quote: ... T cells (1 million cells/ml) were activated with Dynabeads Human T-Activator CD3/CD28 for T Cell Expansion and Activation (Thermo Fisher) in culture medium ...
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bioRxiv - Immunology 2024Quote: ... primed NK cells from PWH were cultured with autologous CD4+ T cells also isolated by negative immunomagnetic selection (DynaBeads Human CD4+ T cell isolation kit, Life technologies) in the presence of 50nM Romidepsin (Selleck Chemicals ...
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bioRxiv - Immunology 2024Quote: ... CD4+ T cells were isolated from the CD8-depleted PBMC fraction using the MagniSort human CD4+ T cell enrichment kit (Thermo Fisher). T cell purity was routinely checked by flow cytometry (>80%) ...
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bioRxiv - Microbiology 2023Quote: HeLa epithelial cells and MelJuSo human melanoma cells were cultured in Gibco Iscove’s Modified Dulbecco’s Medium (IMDM; ThermoFisher Scientific, the Netherlands) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Microbiology 2023Quote: Human lung cancer A549 cells and Chinese hamster ovary (CHO) cells were cultured in DMEM medium (GIBCO, Thermo Fisher Scientific, China) and RPMI1640 medium (GIBCO ...
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bioRxiv - Microbiology 2023Quote: Human lung cancer A549 cells and Chinese hamster ovary (CHO) cells were cultured in DMEM medium (GIBCO, Thermo Fisher Scientific, China) and RPMI1640 medium (GIBCO ...
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bioRxiv - Neuroscience 2023Quote: Low-passage-number Human embryonic kidney cells transformed with SV40 T-antigen (HEK293T) cells were cultured in DMEM (Thermo Fisher Scientific) with 10% fetal bovine serum at 37°C in a humidified atmosphere with 5% CO2 ...
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bioRxiv - Microbiology 2023Quote: ... Madin Darby canine kidney (MDCK) cells and human embryonic kidney 293T cells (ATCC) were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Life Technologies Thermo Fisher) supplemented with 10% (v/v ...
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bioRxiv - Molecular Biology 2020Quote: ... Human transferrin-568 (ThermoFisher) was added at 10μg mL−1 concentration in serum free media and incubated at 37°C to allow for internalization ...
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bioRxiv - Immunology 2021Quote: ... then anti-human (Invitrogen) horseradish peroxidase-conjugated antibodies were diluted 1:5,000 and 50 μL added to each well and incubated at 37°C for 1 h ...
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bioRxiv - Cancer Biology 2022Quote: Probes Hs00171064_m1 (human, ThermoFisher), Mm00440280_g1 (mouse ...
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bioRxiv - Bioengineering 2022Quote: ... Primary human hepatocytes (Gibco) were seeded in the top channel at a density of 3.5 x 106 cells/mL using complete hepatocyte seeding media ...
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bioRxiv - Immunology 2020Quote: ... or anti-human (Invitrogen) horseradish peroxidase-conjugated antibodies were diluted 1:5,000 and 25 μL added to each well and incubated at 37°C for 1 h ...
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bioRxiv - Microbiology 2021Quote: ... or human IgG (Invitrogen) as control ...
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bioRxiv - Bioengineering 2022Quote: ... and human fibronectin (Gibco) at 50 μg/mL in PBS ...
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bioRxiv - Cell Biology 2022Quote: ... human (ThermoFisher Scientific, 902927). Analysis was performed with Transcriptome Analysis Console 4.0 software (Applied Biosystems ...
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bioRxiv - Immunology 2022Quote: ... then anti-human (Invitrogen) horseradish peroxidase-conjugated antibodies were diluted 1:5,000 and 50 μL added to each well and incubated at 37°C for 1 h ...
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bioRxiv - Molecular Biology 2024Quote: ... CD36 (Human tissue Thermofisher: PA1-16813 1:500 ...
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bioRxiv - Developmental Biology 2023Quote: ... 1% human IgG (Invitrogen) in PBS] followed by incubation with primary antibodies ...
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bioRxiv - Cell Biology 2023Quote: ... human plasma (ThermoFisher, #33016015); Laminin 111 ...
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bioRxiv - Immunology 2023Quote: ... A Human ProcartaPlexTM (Invitrogen) immunoassay was additionally used to detect 45 human cytokines ...
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bioRxiv - Immunology 2024Quote: ... Anti-Human-HRP (Invitrogen) was diluted 1:5000 in 1% BSA/PBS ...
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bioRxiv - Cell Biology 2024Quote: ... human recombinant insulin (ThermoFisher) was added at 100 nM for 30 minutes and Smoothened agonist (SAG ...
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bioRxiv - Cancer Biology 2024Quote: ... human CD3-PE (Invitrogen), and mouse CD45-erpCP Cy5.5 (BD Biosciences ...
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bioRxiv - Developmental Biology 2021Quote: ... C2cd6s was subcloned into pcDNA3.1/myc-His A vector (Cat. No. V800-20, Invitrogen). The others were TA-cloned to pcDNA3.1/V5-His TOPO TA vectors (Cat ...
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bioRxiv - Molecular Biology 2021Quote: ... using the Ion PI™ Hi-Q™ OT2 200 kit (Invitrogen; Cat #A26434) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... incubated with DPBS containing 10% HI FBS and 1:1000 Hoechst 33342 (Invitrogen, #H3570) for nuclear staining and mounted onto microscope cover slips with Fluoromount G (Southern Biotech ...
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bioRxiv - Microbiology 2020Quote: ... supplemented with 10% heat-inactivated fetal bovine serum [HI-FBS] (Cat#SH30071.03, Thermo Scientific), penicillin [100 IU/ml]-streptomycin [100 ug/ml] (Quality Biological ...
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bioRxiv - Molecular Biology 2019Quote: ... BAC DNA extraction was performed using Hi-Pure Plasmid DNA Extraction Kit (Invitrogen K210017). Nick translation of BAC DNA was performed using the Nick Translation kit (Roche 11 745 808 910) ...
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bioRxiv - Molecular Biology 2019Quote: ... full-length MmEsco2 was cloned into the pcDNA3.1/myc-His vector (Thermo Fisher Scientific). Subsequently ...
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bioRxiv - Microbiology 2019Quote: ... 6×His-Lsr2 was purified by binding to 1 mL Ni-NTA agarose (Invitrogen), after which the resin was collected and the bound protein was washed with binding buffer supplemented with increasing concentrations of imidazole ...
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bioRxiv - Immunology 2021Quote: ... The extracellular domain of NKp46 was cloned into pcDNA Myc-His 3.1a vector (Invitrogen) or pCMV vector (Addgene plasmid #59314 ...
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bioRxiv - Bioengineering 2020Quote: ... rabbit monoclonal anti-6x-His tag at 1:1,000 (Thermo Fisher Scientific, MA5-33032), and mouse monoclonal anti-β-actin at 1:10,000 (R&D Systems ...
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bioRxiv - Molecular Biology 2021Quote: ... The cleared lysate was incubated with 1mL His-Pur nickel-NTA resin (Thermo Fisher) with rotation at 4 °C for 1 hour ...
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bioRxiv - Molecular Biology 2020Quote: ... ZAP-S and ZAP-L were subcloned into pEF1-V5/His (Thermo Fisher Scientific) via the KpnI/XbaI sites to generate pEF1-ZAP-S-V5/His and pEF1-ZAP-L-V5/His ...
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bioRxiv - Neuroscience 2022Quote: ... the pEx-FGD4-His-V5 vector was generated by Gateway cloning technology (Thermofisher, USA). Transfection experiments were performed using promofectin reagent (#PK-CT-2000-50 ...
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bioRxiv - Synthetic Biology 2022Quote: All antigens were His-tagged and purified using HisPur™ Ni-NTA resin (ThermoFisher). Cell supernatants were diluted with 1/3rd volume wash buffer [20 mM imidazole ...
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bioRxiv - Microbiology 2022Quote: ... or membrane probed with mouse monoclonal 6x-His tag antibody (4A12E4) (Thermofisher, Waltham, MA) (Supplementary Figure 2).
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bioRxiv - Immunology 2022Quote: ... unbiotinylated form were incubated with 2μg/mL anti-His biotin (Invitrogen, MA1-21315-BTIN) for 20 min at room temperature before being used to label the streptavidin beads ...
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Assessment of Human Renal Transporter Based Drug-Drug Interactions Using Proximal Tubule Kidney-ChipbioRxiv - Cell Biology 2022Quote: ... Heat inactivated fetal bovine serum (HI FBS) and trypan blue were procured from Gibco Life Technologies (Waltham ...