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Citations for Thermo Fisher :
2751 - 2800 of 4235 citations for Influenza Virus Hemagglutinin HA H7N9 Fc tag since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... RNA was extracted from 50 μl of virus inoculum suspension using the PureLink Viral RNA/DNA Mini kit (Themo Fisher Scientific), as per manufacturer’s directions.
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bioRxiv - Microbiology 2022Quote: ... and resuspended in thawed Geltrex lactose dehydrogenase elevating virus free (LDEV-free) reduced growth factor basement membrane matrix (Thermo Fisher Scientific). Three 10-μl drops of the matrix-cell suspension were pipetted into wells of a Nunclon Delta 24-well plate (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2022Quote: ... 7 µl total RNA was amplified in a mix containing 5 µl of TaqMan Fast Virus 1-step master mix (Life Technologies), 0.4 µl primer-probe mix for IAV 40 and 9.6 µl distilled water ...
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bioRxiv - Microbiology 2022Quote: Copies of the SARS-CoV-2 N gene (genomic) were measured by qRT-PCR TaqMan Fast Virus 1-step assay (Applied Biosystems). SARS-CoV-2 specific primers and probes from the 2019-nCoV RUO Assay kit (Integrated DNA Technologies ...
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bioRxiv - Microbiology 2022Quote: Copies of SARS-CoV-2 E gene (subgenomic) were measured by qRT-PCR with TaqMan Fast Virus 1-step assay (Thermo Fisher). SARS-CoV-2 specific primers and probes sgLeadSARSCoV2-F 5’-CGATCTCTTGTAGATCTGTTCTC-3’ ...
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bioRxiv - Microbiology 2022Quote: ... The virus-containing samples (50 μl) were serially diluted in 450 μl aliquots of serum-free Opti-Pro medium (Gibco, UK), after which 200 μl from each dilution were added in duplicates to the cells ...
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bioRxiv - Microbiology 2022Quote: ... 5 µl total RNA was amplified in a mix containing 5 µl of TaqMan Fast Virus 1-step master mix (Life Technologies), 1 µl primer-probe mix for β-actin ...
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bioRxiv - Neuroscience 2022Quote: ... HT-1080 cells (ATCC CCL-121; RRID:CVCL_0317, male) used for virus titration were grown and maintained in MEM (Gibco Cat#11095-080) supplemented with 10% FBS ...
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bioRxiv - Microbiology 2023Quote: ... The samples were used for quantitating HuNoV RNA GEs by means of reverse transcription-quantitative PCR (RT-qPCR) analysis using a TaqMan Fast Virus 1-Step Master Mix (Thermo Fisher) and GII specific primer/probe sets ...
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bioRxiv - Neuroscience 2024Quote: ... HEK cells below passage 10 are plated into a 15 cm dish/virus (Fisher 08-772-24) in DMEM-H (DMEM-h (invitrogen 10569010) + NEAA (Invitrogen 11140050) + 10% FBS (invitrogen 16140071 ...
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bioRxiv - Microbiology 2024Quote: ... Virus stocks were grown in M2-10B4 cells (ATCC # CRL-1972) with RPMI 1640 Medium special formulation (Thermo Fisher cat # A1049101) supplemented with 10% fetal bovine serum (Thermo Fisher cat # 12483020 ...
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bioRxiv - Cancer Biology 2024Quote: ... Subsequent qPCR was performed using the TaqMan Fast Virus 1-Step Master Mix and StepOnePlus Real-Time PCR System (Thermo Fisher). The primers purchased from TaqMan were ...
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bioRxiv - Genetics 2024Quote: The derivation of iPSCs was carried out by the NHLBI iPSCs core facility using the Cytotune 2.0 Sendai virus kit (ThermoFisher Scientific, A16517) with a few modifications for high throughput reprogramming essentially as described earlier (19) ...
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bioRxiv - Microbiology 2023Quote: ... Anesthetized mice were inoculated intranasally with 30 μL of virus diluted in chilled 1X PBS (MHV) or administered undiluted in 2% FBS (Gibco, MA)/1X non-essential amino acids (Gibco ...
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bioRxiv - Immunology 2023Quote: ... Both assays were performed using the Taqman Fast Virus 1-Step Master Mix RT-qPCR kit (Applied Biosystems Inc., Carlsbad, CA) on an LC96 instrument (Roche ...
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bioRxiv - Biochemistry 2023Quote: ... the elutes of GlnR147 (his-TEV GlnR C60SL147C) from Ni-NTA column were cleaved with recombinant tobacco-etch virus protease (Life Technologies) overnight at 4 °C ...
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bioRxiv - Biochemistry 2023Quote: ... Sf9 cells were infected (multiplicity of infection of 6 for each virus) with separate baculoviruses (prepared using the Bac-to-Bac Baculovirus Expression System, Invitrogen) containing the CXCL12LRHQ gene as well as the ACKR3 gene (residues 2-362 ...
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bioRxiv - Microbiology 2023Quote: We diluted freshly purified virus samples in DPBS and combined three volumes of the virus with one volume of NuPAGE LDS Sample Buffer (4×; Thermo Fisher) containing 100 mM dithiothreitol ...
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bioRxiv - Immunology 2023Quote: ... 2μg liver RNA per sample was used for qRT-PCR analysis for Plasmodium 18S rRNA using TaqMan Fast Virus 1-Step Master Mix (Applied Biosystems). Data were normalized for input to the GAPDH control for each sample and are presented as ratios of Plasmodium 18s rRNA to GAPDH RNA signals ...
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bioRxiv - Microbiology 2023Quote: ... For intravenous inoculation mice were restrained and injected via tail vein injection with 100 to 200 μl of virus or control inoculum using a 29G insulin syringe (Fisher Scientific). For natural transmission ...
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bioRxiv - Bioengineering 2023Quote: ... The levels of RNA corresponding to the N protein-encoding gene of SARS-CoV-2 were measured using the TaqMan Fast Virus 1-step Master Mix (Thermo Scientific). Each 20-μL reaction mixture contained 5.0 μL of 4× TaqMan Fast Virus 1-Step Master Mix ...
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bioRxiv - Cell Biology 2023Quote: The adeno-associated virus MyoAAV.2A was produced using the AAV-MAX Helper-Free AAV Production System Kit (Thermo Fisher Scientific). At 72 h post-transfection ...
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bioRxiv - Microbiology 2023Quote: ... Viral genome levels were quantified by qPCR using virus specific primer probe sets (described above) and normalised to GAPDH using an Applied Biosystems FAM/MGB probe mix (Thermofisher, 4333764F).
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bioRxiv - Genomics 2023Quote: ... the fibroblasts were thawed and plated at a density of 250K cells/well of 6-well plate and infected with the Cytotune Sendai virus (Life Technologies) per manufacturer’s protocol to initiate reprogramming ...
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bioRxiv - Pathology 2023Quote: ... was reverse transcribed at 37°C for 50 min in a 20 µl reaction mix containing 200U Moloney murine leukemia virus (MMLV) reverse transcriptase (Life Technologies), 100 ng random primers ...
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bioRxiv - Biochemistry 2023Quote: ... Baculoviruses were amplified twice and freshly generated P3 virus was used to infect 1.8-2 L of High Five cells (Thermo Fisher Scientific) at a density of 3-3.5 x106 cells/mL by adding 6 mL virus per 600 mL of cells ...
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bioRxiv - Neuroscience 2024Quote: ... The MS19-ES-H line was reprogrammed from PBMCs from a healthy female donor with a Cytotune Sendai Virus kit (Thermo Fisher).
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bioRxiv - Microbiology 2024Quote: ... isolated RNA was run using a TaqMan Fast Virus One-Step Master Mix (applied Biosystems) on a QuantStudio 3 Flex Real-Time PCR system (Applied Biosystems). RNA standards with known copy numbers were used to generate a standard curve and calculate sample copy numbers ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Patients consent was retrieved and the iPSCs were generated from peripheral blood mononuclear cells (PBMCs) using Sendai virus (CytoTuneTM-iPS 2.0 Sendai Reprogramming Kit, Thermo Fisher Scientific) as previously described ...
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bioRxiv - Neuroscience 2024Quote: ... The fluorescent protein expressed by the virus (either GFP or mCherry) was visualized using an EVOS FL tabletop fluorescent microscope (ThermoFisher Scientific). Images were taken for future reference ...
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bioRxiv - Biophysics 2020Quote: MDA-MB-231 breast cancer cells were cultured in modified Eagle’s medium (MEM-F10, c.c.pro) supplemented with 10% fetal calf serum (FCS, Invitrogen) and 2.5 mM L-glutamin (c.c.pro ...
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bioRxiv - Biophysics 2020Quote: ... cell medium was replaced by 1 ml Leibovitz’s L-15 Medium (c.c.pro) containing 10% FCS and 25 nM Hoechst 33342 nucleic acid stain (Invitrogen) and incubated for 1 h at 37 °C before imaging.
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bioRxiv - Cancer Biology 2021Quote: ... After washing three times with MACS buffer consisting of PBS supplemented with 5 % FCS (Thermo Fisher Scientic) and 2 mM EDTA to remove unbounded cells ...
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bioRxiv - Immunology 2021Quote: ... and 10% fetal calf serum (FCS, Biowest) and minced into pieces of 1 mm3 in PBS (Gibco). The biopsies were enzymatically digested with 1 mg/mL collagenase IV (Sigma ...
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bioRxiv - Molecular Biology 2020Quote: ... USA) supplemented with 10% fetal calf serum (FCS) and 1% penicillin-streptomycin solution (Invitrogen, Carlsbad, CA, USA) and were incubated at 37□ in a humidified atmosphere containing 5% CO2 ...
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bioRxiv - Molecular Biology 2020Quote: ... Cells were pre-incubated with anti-Fc receptor antibody (Thermal Fisher Scientific, Cat. No. 14-9161-73) at 1:20 ratio to block Fc receptor before staining ...
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bioRxiv - Immunology 2021Quote: ... and the absorbance (OD492nm) was measured using a Multiskan-FC microplate reader (Thermo Fisher Scientific, Helsinki, Finland). Ligation of the recombinant proteins was compared to the SUMO control.
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bioRxiv - Immunology 2019Quote: ... resuspended in William’s E medium containing 10% FCS (PAA) and 1% Penicillin-Streptomycin-Glutamine (Thermo Fisher Scientific) and plated ...
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bioRxiv - Microbiology 2019Quote: ... in Dulbecco’s Modified Eagle medium (DME) supplemented with 1% v/v fetal calf serum (FCS) (Invitrogen, Australia) and 1% v/v Glutamax (Invitrogen ...
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bioRxiv - Developmental Biology 2020Quote: ... 1 % non-essential amino acids and sodium pyruvate and 30 % fetal calf serum (FCS) (Gibco, 10270-106). When cells reached confluency ...
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bioRxiv - Neuroscience 2020Quote: ... The absorbance at 450 nm wavelength was read on a microplate reader (Multiskan FC, Thermo Fisher Scientific). Mean values were calculated for triplicate sets of standards and samples ...
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bioRxiv - Biophysics 2020Quote: ... the substrate was washed with cell culture medium (DMEM w/glutamax, 10% FCS, 1% P/S; Gibco). 3T3 fibroblasts (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2021Quote: ... Recombinant Fc linked DR5 variants were biotinylated using EZ-Link Sulfo-NHS-SS-Biotin (Thermo Scientific 21331) following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2020Quote: ... Absorbance was then measured at 595 nm using a Multiskan™ FC microplate photometer (Thermo Fisher Scientific).
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bioRxiv - Microbiology 2020Quote: ... supplemented with 10% FCS and antibiotics (100 units/mL penicillin, 100 mg/mL streptomycin, GIBCO, Life Technologies). Mammalian cells were incubated at 35°C in a humidified 5% CO2 incubator and insect cells at 28°C ...
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bioRxiv - Microbiology 2020Quote: ... supplemented with 10% FCS and antibiotics (100 units/mL penicillin, 100 mg/mL streptomycin, GIBCO, Life Technologies). Mammalian cells were incubated at 35°C in a humidified 5% CO2 incubator and insect cells at 28°C ...
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bioRxiv - Microbiology 2020Quote: ... the absorbance at 492 nm was measured with a microplate photometer (Multiskan FC, Thermo Fisher Scientific, USA).
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bioRxiv - Cell Biology 2021Quote: ... supplemented with 10% heat-inactivated fetal calf serum (FCS) (Cytiva) and 2mM L-glutamine (Thermo Fisher Scientific) at 37°C in humidified 5% CO2 ...
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bioRxiv - Immunology 2022Quote: ... the enzymatic dissociation of the lamina propria was quenched by addition of 1ml of 100% FCS (ThermoFisher) and 80 μL of 0.5M EDTA and placed on ice for five minutes ...
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bioRxiv - Immunology 2022Quote: ... After another round of Fc blocking on ice for 15 min with sera from mouse (Thermo Fisher) and rat (Thermo Fisher) ...